5212005

2,6-diamino-4-(3-chlorophenyl)-4H-thiopyran-3,5-dicarbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2073
Screen concentration 126.0 μM
Source Chembridge (Fragment library)
PubChem CID 691789
SMILES C1=CC(=CC(=C1)Cl)C2C(=C(SC(=C2C#N)N)N)C#N
Standardized SMILES NC1=C(C#N)C(C(=C(N)S1)C#N)c2cccc(Cl)c2
Molecular weight 288.7554
ALogP 2.13
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.89
% growth inhibition (Hom. pool) 9.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 691789
Download HIP data (tab-delimited text)  (excel)
Gene:DML1(YMR211W)|FD-Score:3.18|P-value:7.40E-4|Clearance:0.05||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG20(YJL167W)|FD-Score:3.83|P-value:6.45E-5|Clearance:0.01||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:ESP1(YGR098C)|FD-Score:-3.98|P-value:3.48E-5|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FRQ1(YDR373W)|FD-Score:4.66|P-value:1.59E-6|Clearance:0.83||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:GLC7(YER133W)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.4|P-value:3.34E-4|Clearance:0.04||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:NUP159(YIL115C)|FD-Score:-3.74|P-value:9.22E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP49(YGL172W)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.02||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP85(YJR042W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:REB1(YBR049C)|FD-Score:-3.43|P-value:3.06E-4|Clearance:0||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RET1(YOR207C)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.01||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPN5(YDL147W)|FD-Score:3.47|P-value:2.63E-4|Clearance:0.03||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPO26(YPR187W)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPT5(YOR117W)|FD-Score:5.52|P-value:1.72E-8|Clearance:0.86||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RPT6(YGL048C)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.03||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRP7(YCL031C)|FD-Score:-3.77|P-value:8.31E-5|Clearance:0||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SAM35(YHR083W)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SEC27(YGL137W)|FD-Score:3.13|P-value:8.81E-4|Clearance:0.08||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SSY1(YDR160W)|FD-Score:3.81|P-value:6.83E-5|Clearance:0||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TIF35(YDR429C)|FD-Score:-4.27|P-value:9.96E-6|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOA1(YOR194C)|FD-Score:3.36|P-value:3.91E-4|Clearance:0.18||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:YDL152W(YDL152W_d)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YJR141W(YJR141W_p)|FD-Score:3.81|P-value:6.91E-5|Clearance:0.23||SGD DESC:Essential protein of unknown function Gene:DML1(YMR211W)|FD-Score:3.18|P-value:7.40E-4|Clearance:0.05||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG20(YJL167W)|FD-Score:3.83|P-value:6.45E-5|Clearance:0.01||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:ESP1(YGR098C)|FD-Score:-3.98|P-value:3.48E-5|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FRQ1(YDR373W)|FD-Score:4.66|P-value:1.59E-6|Clearance:0.83||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:GLC7(YER133W)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.4|P-value:3.34E-4|Clearance:0.04||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:NUP159(YIL115C)|FD-Score:-3.74|P-value:9.22E-5|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP49(YGL172W)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.02||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP85(YJR042W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:REB1(YBR049C)|FD-Score:-3.43|P-value:3.06E-4|Clearance:0||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RET1(YOR207C)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.01||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPN5(YDL147W)|FD-Score:3.47|P-value:2.63E-4|Clearance:0.03||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPO26(YPR187W)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPT5(YOR117W)|FD-Score:5.52|P-value:1.72E-8|Clearance:0.86||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RPT6(YGL048C)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.03||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRP7(YCL031C)|FD-Score:-3.77|P-value:8.31E-5|Clearance:0||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SAM35(YHR083W)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SEC27(YGL137W)|FD-Score:3.13|P-value:8.81E-4|Clearance:0.08||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SSY1(YDR160W)|FD-Score:3.81|P-value:6.83E-5|Clearance:0||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:TIF35(YDR429C)|FD-Score:-4.27|P-value:9.96E-6|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOA1(YOR194C)|FD-Score:3.36|P-value:3.91E-4|Clearance:0.18||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:YDL152W(YDL152W_d)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YJR141W(YJR141W_p)|FD-Score:3.81|P-value:6.91E-5|Clearance:0.23||SGD DESC:Essential protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 691789
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:BIK1(YCL029C)|FD-Score:-3.78|P-value:7.97E-5||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:CRG1(YHR209W)|FD-Score:4.94|P-value:3.97E-7||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DBF2(YGR092W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DSE4(YNR067C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:DSK2(YMR276W)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:FUR4(YBR021W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GEA2(YEL022W)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GIC1(YHR061C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:GRX7(YBR014C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAP4(YKL109W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HMO1(YDR174W)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IMP2(YMR035W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:KIN82(YCR091W)|FD-Score:-3.75|P-value:8.97E-5||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:KRE28(YDR532C)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:KRE6(YPR159W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LOC1(YFR001W)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAG1(YER142C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MFG1(YDL233W_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MRP8(YKL142W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MSC7(YHR039C)|FD-Score:-5.12|P-value:1.55E-7||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NHP10(YDL002C)|FD-Score:-3.24|P-value:5.96E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:OCA6(YDR067C_p)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PEX6(YNL329C)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:POL32(YJR043C)|FD-Score:4.8|P-value:8.08E-7||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:RGT1(YKL038W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RHO4(YKR055W)|FD-Score:-3.88|P-value:5.18E-5||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPN4(YDL020C)|FD-Score:4.87|P-value:5.63E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS27B(YHR021C)|FD-Score:-3.87|P-value:5.54E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:SDS3(YIL084C)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SIR1(YKR101W)|FD-Score:-4.57|P-value:2.43E-6||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SLG1(YOR008C)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SSZ1(YHR064C)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SUR2(YDR297W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUV3(YPL029W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TCB3(YML072C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TGL2(YDR058C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TSC3(YBR058C-A)|FD-Score:6.35|P-value:1.11E-10||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA9(YCL005W-A)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YIL096C(YIL096C_p)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function Gene:YMR153C-A(YMR153C-A_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YNL058C(YNL058C_p)|FD-Score:4.34|P-value:7.17E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOL037C(YOL037C_d)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOL106W(YOL106W_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR316C-A(YOR316C-A_p)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR196W(YPR196W_p)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YRM1(YOR172W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes Gene:ADK1(YDR226W)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:BIK1(YCL029C)|FD-Score:-3.78|P-value:7.97E-5||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:CRG1(YHR209W)|FD-Score:4.94|P-value:3.97E-7||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DBF2(YGR092W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DSE4(YNR067C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:DSK2(YMR276W)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:FUR4(YBR021W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GEA2(YEL022W)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GIC1(YHR061C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:GRX7(YBR014C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAP4(YKL109W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HMO1(YDR174W)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IMP2(YMR035W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:KIN82(YCR091W)|FD-Score:-3.75|P-value:8.97E-5||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:KRE28(YDR532C)|FD-Score:-4.62|P-value:1.96E-6||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:KRE6(YPR159W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LOC1(YFR001W)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAG1(YER142C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MFG1(YDL233W_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MRP8(YKL142W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MSC7(YHR039C)|FD-Score:-5.12|P-value:1.55E-7||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NHP10(YDL002C)|FD-Score:-3.24|P-value:5.96E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:OCA6(YDR067C_p)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PEX6(YNL329C)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:POL32(YJR043C)|FD-Score:4.8|P-value:8.08E-7||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:RGT1(YKL038W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RHO4(YKR055W)|FD-Score:-3.88|P-value:5.18E-5||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPN4(YDL020C)|FD-Score:4.87|P-value:5.63E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS27B(YHR021C)|FD-Score:-3.87|P-value:5.54E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:SDS3(YIL084C)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SIR1(YKR101W)|FD-Score:-4.57|P-value:2.43E-6||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SLG1(YOR008C)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SSZ1(YHR064C)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SUR2(YDR297W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUV3(YPL029W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TCB3(YML072C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TGL2(YDR058C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TSC3(YBR058C-A)|FD-Score:6.35|P-value:1.11E-10||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA9(YCL005W-A)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YIL096C(YIL096C_p)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function Gene:YMR153C-A(YMR153C-A_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YNL058C(YNL058C_p)|FD-Score:4.34|P-value:7.17E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOL037C(YOL037C_d)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOL106W(YOL106W_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR316C-A(YOR316C-A_p)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR196W(YPR196W_p)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YRM1(YOR172W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR117W5.521.72E-80.86RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YDR373W4.661.59E-60.83FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YJL167W3.836.45E-50.01ERG20Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
YDR160W3.816.83E-50.00SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YJR141W_p3.816.91E-50.23YJR141W_pEssential protein of unknown function
YPR187W3.581.74E-40.08RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YDL152W_d3.502.36E-40.01YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YGL172W3.482.47E-40.02NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YDL147W3.472.63E-40.03RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YGL048C3.442.91E-40.03RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YOR207C3.413.22E-40.01RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDR047W3.403.34E-40.04HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YOR194C3.363.91E-40.18TOA1TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
YMR211W3.187.40E-40.05DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YGL137W3.138.81E-40.08SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A6.351.11E-10TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YOR316C-A_p5.101.66E-7YOR316C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YHR209W4.943.97E-7CRG1S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
YDL020C4.875.63E-7RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YJR043C4.808.08E-7POL32Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
YNL058C_p4.347.17E-6YNL058C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene
YMR153C-A_d4.231.17E-5YMR153C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53
YIL134C-A_p3.865.70E-5YIL134C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YEL022W3.846.05E-5GEA2Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
YMR001C-A_p3.502.29E-4YMR001C-A_pPutative protein of unknown function
YPL029W3.492.39E-4SUV3ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron
YDR297W3.482.51E-4SUR2Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
YMR035W3.393.44E-4IMP2Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
YDL233W_p3.334.28E-4MFG1_pRegulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene
YOL106W_d3.314.74E-4YOL106W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2073
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1911.53E-49SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.126761TSC3-RPN4
0.1779.27E-43SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0684932
0.1758.06E-42SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0625TSC3-RPN4
0.1739.19E-41SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.340426TSC3-RPN4
0.1721.48E-40SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0833333TSC3-RPN4
0.1693.49E-39SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.08
0.1696.57E-39SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.0972222
0.1632.47E-36SGTC_1634st006576 41.0 μMTimTec (Natural product derivative library)39153560.186667TSC3-RPN4
0.1611.01E-35SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0727273
0.1582.41E-34SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.150943TSC3-RPN4
0.1561.68E-33SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0895522TSC3-RPN4
0.1529.84E-32SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0701754
0.1505.36E-31SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.140351TSC3-RPN4
0.1447.83E-29SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0327869TSC3-RPN4
0.1441.13E-28SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0847458

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_20755212009200 μM0.684211780898Chembridge (Fragment library)272.3007631.66826
SGTC_20785212016200 μM0.6666671899708Chembridge (Fragment library)288.755362.1272560S ribosome export
SGTC_20865274770176.62 μM0.657143689278Chembridge (Fragment library)254.31031.46325
SGTC_20885278280127.39 μM0.634146791502Chembridge (Fragment library)284.336281.44726
SGTC_20765212012200 μM0.560976604802Chembridge (Fragment library)255.298360.31226
SGTC_20775212015200 μM0.55780900Chembridge (Fragment library)288.755362.12725
SGTC_21115486399200 μM0.5689674Chembridge (Fragment library)282.363462.40525
SGTC_2071523095139.92 μM0.476191799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4
SGTC_2074521200775.4 μM0.47619758611Chembridge (Fragment library)272.3007631.66826
SGTC_20435101342149 μM0.454545678451Chembridge (Fragment library)282.363462.43525SWF1 & branched chain AA biosynthesis