5212009

2,6-diamino-4-(3-fluorophenyl)-4H-thiopyran-3,5-dicarbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2075
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 780898
SMILES C1=CC(=CC(=C1)F)C2C(=C(SC(=C2C#N)N)N)C#N
Standardized SMILES NC1=C(C#N)C(C(=C(N)S1)C#N)c2cccc(F)c2
Molecular weight 272.3008
ALogP 1.67
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.88
% growth inhibition (Hom. pool) 3.22


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 780898
Download HIP data (tab-delimited text)  (excel)
Gene:CDC5(YMR001C)|FD-Score:3.75|P-value:8.72E-5|Clearance:0.14||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:CDC60(YPL160W)|FD-Score:4.31|P-value:8.28E-6|Clearance:0.41||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:FRQ1(YDR373W)|FD-Score:-4.86|P-value:5.87E-7|Clearance:0||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:IPL1(YPL209C)|FD-Score:4.3|P-value:8.38E-6|Clearance:0.41||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:LSG1(YGL099W)|FD-Score:4.7|P-value:1.28E-6|Clearance:0.41||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM10(YIL150C)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MED4(YOR174W)|FD-Score:3.76|P-value:8.47E-5|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:PAP1(YKR002W)|FD-Score:-3.14|P-value:8.39E-4|Clearance:0||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PRE10(YOR362C)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RER2(YBR002C)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.29||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPC10(YHR143W-A)|FD-Score:-4.46|P-value:4.05E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPT6(YGL048C)|FD-Score:5.39|P-value:3.44E-8|Clearance:0.41||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:SEC17(YBL050W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.15||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SSS1(YDR086C)|FD-Score:5.02|P-value:2.63E-7|Clearance:0.41||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:UBA1(YKL210W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.11||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YEF3(YLR249W)|FD-Score:4.17|P-value:1.50E-5|Clearance:0.41||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:CDC5(YMR001C)|FD-Score:3.75|P-value:8.72E-5|Clearance:0.14||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:CDC60(YPL160W)|FD-Score:4.31|P-value:8.28E-6|Clearance:0.41||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:FRQ1(YDR373W)|FD-Score:-4.86|P-value:5.87E-7|Clearance:0||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:IPL1(YPL209C)|FD-Score:4.3|P-value:8.38E-6|Clearance:0.41||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:LSG1(YGL099W)|FD-Score:4.7|P-value:1.28E-6|Clearance:0.41||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM10(YIL150C)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MED4(YOR174W)|FD-Score:3.76|P-value:8.47E-5|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:PAP1(YKR002W)|FD-Score:-3.14|P-value:8.39E-4|Clearance:0||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PRE10(YOR362C)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RER2(YBR002C)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.29||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPC10(YHR143W-A)|FD-Score:-4.46|P-value:4.05E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPT6(YGL048C)|FD-Score:5.39|P-value:3.44E-8|Clearance:0.41||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:SEC17(YBL050W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.15||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SSS1(YDR086C)|FD-Score:5.02|P-value:2.63E-7|Clearance:0.41||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:UBA1(YKL210W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.11||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YEF3(YLR249W)|FD-Score:4.17|P-value:1.50E-5|Clearance:0.41||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 780898
Download HOP data (tab-delimited text)  (excel)
Gene:ADY2(YCR010C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AIM45(YPR004C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ASN1(YPR145W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BEM3(YPL115C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:CBF1(YJR060W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CSM4(YPL200W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DIF1(YLR437C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DUF1(YOL087C)|FD-Score:-3.78|P-value:7.75E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EPT1(YHR123W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:ERG28(YER044C)|FD-Score:-3.73|P-value:9.69E-5||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FAB1(YFR019W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FAR11(YNL127W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FRT1(YOR324C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:HHY1(YEL059W_d)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:INP51(YIL002C)|FD-Score:5.3|P-value:5.86E-8||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC15(YPL017C)|FD-Score:-5.21|P-value:9.27E-8||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ISY1(YJR050W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:IXR1(YKL032C)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LSM1(YJL124C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MDY2(YOL111C)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MKK1(YOR231W)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MST1(YKL194C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC2(YKL098W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NIF3(YGL221C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OAF1(YAL051W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OMA1(YKR087C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PET117(YER058W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PET191(YJR034W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PIM1(YBL022C)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:RPB9(YGL070C)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL6B(YLR448W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS29B(YDL061C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RVS161(YCR009C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SEC22(YLR268W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SSZ1(YHR064C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SUR1(YPL057C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:TCB3(YML072C)|FD-Score:-7.13|P-value:5.01E-13||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRS33(YOR115C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TUL1(YKL034W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBX2(YML013W)|FD-Score:-4.7|P-value:1.31E-6||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:URA10(YMR271C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VMA11(YPL234C)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS63(YLR261C_d)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS75(YNL246W)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YBR178W(YBR178W_d)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YER187W(YER187W_p)|FD-Score:4.51|P-value:3.20E-6||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL159W(YGL159W_p)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGL177W(YGL177W_d)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL169C(YKL169C_d)|FD-Score:-4|P-value:3.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR194C(YLR194C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function Gene:YML090W(YML090W_d)|FD-Score:3.73|P-value:9.65E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR315W(YMR315W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YMR316C-A(YMR316C-A_p)|FD-Score:5.04|P-value:2.33E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNR029C(YNR029C_p)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR024W(YOR024W_d)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR196W(YPR196W_p)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:ADY2(YCR010C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AIM45(YPR004C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ASN1(YPR145W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BEM3(YPL115C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:CBF1(YJR060W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CSM4(YPL200W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DIF1(YLR437C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DUF1(YOL087C)|FD-Score:-3.78|P-value:7.75E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EPT1(YHR123W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:ERG28(YER044C)|FD-Score:-3.73|P-value:9.69E-5||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FAB1(YFR019W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FAR11(YNL127W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FRT1(YOR324C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:HHY1(YEL059W_d)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:INP51(YIL002C)|FD-Score:5.3|P-value:5.86E-8||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC15(YPL017C)|FD-Score:-5.21|P-value:9.27E-8||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:ISY1(YJR050W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:IXR1(YKL032C)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LSM1(YJL124C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MDY2(YOL111C)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MKK1(YOR231W)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MST1(YKL194C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC2(YKL098W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NIF3(YGL221C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OAF1(YAL051W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OMA1(YKR087C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PET117(YER058W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PET191(YJR034W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PIM1(YBL022C)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:RPB9(YGL070C)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL6B(YLR448W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS29B(YDL061C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RVS161(YCR009C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SEC22(YLR268W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SSZ1(YHR064C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SUR1(YPL057C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:TCB3(YML072C)|FD-Score:-7.13|P-value:5.01E-13||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRS33(YOR115C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TUL1(YKL034W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBX2(YML013W)|FD-Score:-4.7|P-value:1.31E-6||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:URA10(YMR271C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VMA11(YPL234C)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS63(YLR261C_d)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS75(YNL246W)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YBR178W(YBR178W_d)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YER187W(YER187W_p)|FD-Score:4.51|P-value:3.20E-6||SGD DESC:Putative protein of unknown function; induced in respiratory-deficient cells Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL159W(YGL159W_p)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGL177W(YGL177W_d)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL169C(YKL169C_d)|FD-Score:-4|P-value:3.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR194C(YLR194C)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function Gene:YML090W(YML090W_d)|FD-Score:3.73|P-value:9.65E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR315W(YMR315W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YMR316C-A(YMR316C-A_p)|FD-Score:5.04|P-value:2.33E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNR029C(YNR029C_p)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR024W(YOR024W_d)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR196W(YPR196W_p)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Putative maltose-responsive transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL048C5.393.44E-80.41RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YDR086C5.022.63E-70.41SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YGL099W4.701.28E-60.41LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YPL160W4.318.28E-60.41CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YPL209C4.308.38E-60.41IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
YLR249W4.171.50E-50.41YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YOR174W3.768.47E-50.01MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YMR001C3.758.72E-50.14CDC5Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate
YBR002C3.611.52E-40.29RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YBL050W3.324.45E-40.15SEC17Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP
YKL210W3.187.46E-40.12UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YPL237W3.060.001100.03SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YPL011C3.030.001230.04TAF3TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
YOR254C2.990.001410.12SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YBL077W_d2.870.002060.03YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL002C5.305.86E-8INP51Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
YPL115C5.191.06E-7BEM3Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
YMR316C-A_p5.042.33E-7YMR316C-A_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W
YER187W_p4.513.20E-6YER187W_pPutative protein of unknown function; induced in respiratory-deficient cells
YKL032C4.289.51E-6IXR1Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b
YDL061C4.261.00E-5RPS29BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
YKL194C4.052.59E-5MST1Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro
YMR271C3.993.30E-5URA10Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
YOL111C3.768.37E-5MDY2Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes
YHR139C-A_d3.758.68E-5YHR139C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR024W_d3.758.92E-5YOR024W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YML090W_d3.739.65E-5YML090W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source
YOR115C3.681.16E-4TRS33One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YOR324C3.621.50E-4FRT1Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication
YLR264C-A_p3.601.61E-4YLR264C-A_pPutative protein of unknown function

GO enrichment analysis for SGTC_2075
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1343.79E-25SGTC_20745212007 75.4 μMChembridge (Fragment library)7586110.55
0.0984.77E-14SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.1206960S ribosome export
0.0805.93E-10SGTC_2716st080858 38.6 μMTimTec (Natural product derivative library)52960500.12560S ribosome export
0.0781.93E-9SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.068493260S ribosome export
0.0757.71E-9SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.071428660S ribosome export
0.0757.81E-9SGTC_13933470-5649 58.2 μMChemDiv (Drug-like library)41083950.17460360S ribosome export
0.0741.01E-8SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.062560S ribosome export
0.0722.79E-8SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.060606160S ribosome export
0.0681.87E-7SGTC_30349090421 49.5 μMChembridge (Drug-like library)171737610.069444460S ribosome export
0.0682.05E-7SGTC_1675st018485 24.5 μMTimTec (Natural product derivative library)33230240.029411860S ribosome export
0.0672.23E-7SGTC_5794491-1009 110.0 μMChemDiv (Drug-like library)12710410.081967260S ribosome export
0.0672.56E-7SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.062560S ribosome export
0.0663.58E-7SGTC_32999121982 43.1 μMChembridge (Drug-like library)175946040.0434783
0.0664.00E-7SGTC_20295175081 200.0 μMChembridge (Fragment library)13808760.0925926
0.0655.27E-7SGTC_5700987-0065 62.6 μMChemDiv (Drug-like library)31044280.061538560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20735212005126 μM0.684211691789Chembridge (Fragment library)288.755362.12725
SGTC_20865274770176.62 μM0.657143689278Chembridge (Fragment library)254.31031.46325
SGTC_20885278280127.39 μM0.634146791502Chembridge (Fragment library)284.336281.44726
SGTC_20765212012200 μM0.560976604802Chembridge (Fragment library)255.298360.31226
SGTC_2074521200775.4 μM0.55758611Chembridge (Fragment library)272.3007631.66826
SGTC_20785212016200 μM0.5384621899708Chembridge (Fragment library)288.755362.1272560S ribosome export
SGTC_21115486399200 μM0.5689674Chembridge (Fragment library)282.363462.40525
SGTC_2071523095139.92 μM0.476191799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4
SGTC_20775212015200 μM0.47619780900Chembridge (Fragment library)288.755362.12725
SGTC_20435101342149 μM0.454545678451Chembridge (Fragment library)282.363462.43525SWF1 & branched chain AA biosynthesis