5212012

2,6-diamino-4-pyridin-3-yl-4H-thiopyran-3,5-dicarbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2076
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 604802
SMILES C1=CC(=CN=C1)C2C(=C(SC(=C2C#N)N)N)C#N
Standardized SMILES NC1=C(C#N)C(C(=C(N)S1)C#N)c2cccnc2
Molecular weight 255.2984
ALogP 0.31
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.27
% growth inhibition (Hom. pool) 1.13


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 604802
Download HIP data (tab-delimited text)  (excel)
Gene:CDC23(YHR166C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.37||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:POL5(YEL055C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.37||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:QRI1(YDL103C)|FD-Score:3.8|P-value:7.22E-5|Clearance:0.37||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RIB5(YBR256C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.37||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPC10(YHR143W-A)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:SEC23(YPR181C)|FD-Score:-3.35|P-value:3.97E-4|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC7(YDR170C)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:YPR177C(YPR177C_d)|FD-Score:-3.35|P-value:4.03E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:CDC23(YHR166C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.37||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:POL5(YEL055C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.37||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:QRI1(YDL103C)|FD-Score:3.8|P-value:7.22E-5|Clearance:0.37||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RIB5(YBR256C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.37||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPC10(YHR143W-A)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:SEC23(YPR181C)|FD-Score:-3.35|P-value:3.97E-4|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC7(YDR170C)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:YPR177C(YPR177C_d)|FD-Score:-3.35|P-value:4.03E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 604802
Download HOP data (tab-delimited text)  (excel)
Gene:BOP3(YNL042W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:CHK1(YBR274W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase Gene:CYC2(YOR037W)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) Gene:DBP1(YPL119C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:ECM14(YHR132C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ERV25(YML012W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:GCN4(YEL009C)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HNT3(YOR258W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:MDY2(YOL111C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MLP1(YKR095W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MOT3(YMR070W)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:NQM1(YGR043C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:RAX2(YLR084C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:REG1(YDR028C)|FD-Score:4.43|P-value:4.68E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:SLX9(YGR081C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SPO23(YBR250W)|FD-Score:-3.81|P-value:6.87E-5||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:THP2(YHR167W)|FD-Score:5.2|P-value:9.84E-8||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TRM12(YML005W)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:UBP1(YDL122W)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VEL1(YGL258W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS64(YDR200C)|FD-Score:-3.95|P-value:3.90E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL001W-B(YCL001W-B_p)|FD-Score:-4.37|P-value:6.14E-6||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YDL183C(YDL183C)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR271C(YDR271C_d)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL163C(YJL163C_p)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Putative protein of unknown function Gene:YJR087W(YJR087W_d)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Gene:YKL169C(YKL169C_d)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YML034C-A(YML034C-A_d)|FD-Score:4|P-value:3.22E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR122C(YMR122C_d)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR052C(YOR052C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR199W(YOR199W_d)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPS1(YLR120C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:BOP3(YNL042W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:CHK1(YBR274W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase Gene:CYC2(YOR037W)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) Gene:DBP1(YPL119C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:ECM14(YHR132C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ERV25(YML012W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:GCN4(YEL009C)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HNT3(YOR258W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:MDY2(YOL111C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MLP1(YKR095W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MOT3(YMR070W)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:NQM1(YGR043C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:RAX2(YLR084C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:REG1(YDR028C)|FD-Score:4.43|P-value:4.68E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:SLX9(YGR081C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SPO23(YBR250W)|FD-Score:-3.81|P-value:6.87E-5||SGD DESC:Protein of unknown function; associates with meiosis-specific protein Spo1p Gene:THP2(YHR167W)|FD-Score:5.2|P-value:9.84E-8||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TRM12(YML005W)|FD-Score:-3.29|P-value:5.04E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:UBP1(YDL122W)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VEL1(YGL258W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS64(YDR200C)|FD-Score:-3.95|P-value:3.90E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL001W-B(YCL001W-B_p)|FD-Score:-4.37|P-value:6.14E-6||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YDL183C(YDL183C)|FD-Score:-3.14|P-value:8.52E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR271C(YDR271C_d)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL163C(YJL163C_p)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Putative protein of unknown function Gene:YJR087W(YJR087W_d)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Gene:YKL169C(YKL169C_d)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YML034C-A(YML034C-A_d)|FD-Score:4|P-value:3.22E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR122C(YMR122C_d)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR052C(YOR052C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR199W(YOR199W_d)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPS1(YLR120C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL103C3.807.22E-50.37QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YHR166C3.532.09E-40.37CDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YBR256C3.442.89E-40.37RIB5Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
YEL055C3.413.23E-40.37POL5DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
YNL103W3.040.001180.02MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YKL022C3.030.001240.09CDC16Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
YDR299W2.930.001680.01BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YDR118W2.930.001710.01APC4Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
YGL155W2.920.001750.20CDC43Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
YBL004W2.720.003220.01UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YML126C2.720.003280.07ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YGR082W2.650.004050.06TOM20Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
YGL061C2.580.004880.05DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YDR013W2.540.005590.07PSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YMR290C2.470.006740.04HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR167W5.209.84E-8THP2Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
YDR028C4.434.68E-6REG1Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
YML012W4.298.99E-6ERV25Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport
YML034C-A_d4.003.22E-5YML034C-A_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL122W3.993.36E-5UBP1Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
YPL119C3.904.90E-5DBP1Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress
YOR037W3.739.51E-5CYC2Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
YGR043C3.661.25E-4NQM1Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift
YEL009C3.591.62E-4GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YGL258W3.522.15E-4VEL1Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C
YDR271C_d3.522.16E-4YDR271C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
YMR122C_d3.492.40E-4YMR122C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHR132C3.462.66E-4ECM14Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly
YMR070W3.383.68E-4MOT3Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
YHR139C-A_d3.344.13E-4YHR139C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2076
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0764.66E-9SGTC_33139133968 57.0 μMChembridge (Drug-like library)14946430.0779221
0.0756.71E-9SGTC_5623772-2733 112.0 μMChemDiv (Drug-like library)28768740.0704225ERG2
0.0759.25E-9SGTC_2723salbutamol 83.6 μMNIH Clinical Collection98842330.0483871
0.0654.99E-7SGTC_1994063-0024 41.6 μMChemDiv (Drug-like library)3062150.0483871
0.0631.27E-6SGTC_32849108171 64.9 μMChembridge (Drug-like library)86875050.0519481
0.0621.75E-6SGTC_20685226020 147.0 μMChembridge (Fragment library)18105090.0545455
0.0622.07E-6SGTC_1793st048256 72.2 μMTimTec (Natural product derivative library)6720220.04
0.0603.55E-6SGTC_7814335-2806 94.4 μMChemDiv (Drug-like library)8452540.0909091
0.0603.82E-6SGTC_20645220758 200.0 μMChembridge (Fragment library)7885180.078125
0.0595.44E-6SGTC_22127123476 200.0 μMChembridge (Fragment library)10857150.0909091
0.0561.66E-5SGTC_22847945075 39.2 μMChembridge (Fragment library)12454020.0923077mitochondrial processes
0.0552.16E-5SGTC_20615245884 81.0 μMChembridge (Fragment library)5738630.107143tubulin folding & SWR complex
0.0552.32E-5SGTC_33379144342 33.5 μMChembridge (Drug-like library)176829490.169231
0.0552.47E-5SGTC_18825615643 20.0 μMMiscellaneous22530750.0649351TRP & mitochondrial translation
0.0542.86E-5SGTC_15998-chlro-1-tetrahydronorharmanone 90.6 μMTimTec (Pure natural product library)41748400.0645161

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20865274770176.62 μM0.657143689278Chembridge (Fragment library)254.31031.46325
SGTC_20735212005126 μM0.560976691789Chembridge (Fragment library)288.755362.12725
SGTC_20755212009200 μM0.560976780898Chembridge (Fragment library)272.3007631.66826
SGTC_20785212016200 μM0.5384621899708Chembridge (Fragment library)288.755362.1272560S ribosome export
SGTC_20885278280127.39 μM0.522727791502Chembridge (Fragment library)284.336281.44726
SGTC_21115486399200 μM0.5689674Chembridge (Fragment library)282.363462.40525
SGTC_2071523095139.92 μM0.476191799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4
SGTC_2074521200775.4 μM0.47619758611Chembridge (Fragment library)272.3007631.66826
SGTC_20775212015200 μM0.47619780900Chembridge (Fragment library)288.755362.12725
SGTC_2084530607860.64 μM0.431818679340Chembridge (Fragment library)258.2990.79326