5274770

2,6-diamino-4-phenyl-4H-thiopyran-3,5-dicarbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2086
Screen concentration 176.6 μM
Source Chembridge (Fragment library)
PubChem CID 689278
SMILES C1=CC=C(C=C1)C2C(=C(SC(=C2C#N)N)N)C#N
Standardized SMILES NC1=C(C#N)C(C(=C(N)S1)C#N)c2ccccc2
Molecular weight 254.3103
ALogP 1.46
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.56
% growth inhibition (Hom. pool) 4.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 689278
Download HIP data (tab-delimited text)  (excel)
Gene:ERB1(YMR049C)|FD-Score:4.52|P-value:3.02E-6|Clearance:0.16||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:FOL3(YMR113W)|FD-Score:4.81|P-value:7.49E-7|Clearance:0.15||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:GPI18(YBR004C)|FD-Score:5.37|P-value:3.93E-8|Clearance:0.56||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:HSH49(YOR319W)|FD-Score:5.77|P-value:3.89E-9|Clearance:0.56||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSM4(YER112W)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.07||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAS2(YHR024C)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.06||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NIC96(YFR002W)|FD-Score:4.66|P-value:1.60E-6|Clearance:0.04||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NMD3(YHR170W)|FD-Score:4.37|P-value:6.34E-6|Clearance:0.25||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE3(YDR288W)|FD-Score:-3.1|P-value:9.74E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PRE5(YMR314W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP24(YMR268C)|FD-Score:5.51|P-value:1.75E-8|Clearance:0.56||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP9(YDL030W)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RET2(YFR051C)|FD-Score:-4.48|P-value:3.67E-6|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPP1(YHR062C)|FD-Score:5.95|P-value:1.32E-9|Clearance:0.56||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.18||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC9(YGR009C)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.16||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SUI2(YJR007W)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.24||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFB3(YDR460W)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TID3(YIL144W)|FD-Score:4.62|P-value:1.95E-6|Clearance:0.09||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YDL152W(YDL152W_d)|FD-Score:4.1|P-value:2.07E-5|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YIF1(YNL263C)|FD-Score:-3.16|P-value:7.90E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:YLR379W(YLR379W_d)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C Gene:ERB1(YMR049C)|FD-Score:4.52|P-value:3.02E-6|Clearance:0.16||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:FOL3(YMR113W)|FD-Score:4.81|P-value:7.49E-7|Clearance:0.15||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:GPI18(YBR004C)|FD-Score:5.37|P-value:3.93E-8|Clearance:0.56||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:HSH49(YOR319W)|FD-Score:5.77|P-value:3.89E-9|Clearance:0.56||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSM4(YER112W)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.07||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAS2(YHR024C)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.06||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NIC96(YFR002W)|FD-Score:4.66|P-value:1.60E-6|Clearance:0.04||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NMD3(YHR170W)|FD-Score:4.37|P-value:6.34E-6|Clearance:0.25||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE3(YDR288W)|FD-Score:-3.1|P-value:9.74E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PRE5(YMR314W)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP24(YMR268C)|FD-Score:5.51|P-value:1.75E-8|Clearance:0.56||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP9(YDL030W)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RET2(YFR051C)|FD-Score:-4.48|P-value:3.67E-6|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPP1(YHR062C)|FD-Score:5.95|P-value:1.32E-9|Clearance:0.56||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.18||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC9(YGR009C)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.16||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SUI2(YJR007W)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.24||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFB3(YDR460W)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TID3(YIL144W)|FD-Score:4.62|P-value:1.95E-6|Clearance:0.09||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YDL152W(YDL152W_d)|FD-Score:4.1|P-value:2.07E-5|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YIF1(YNL263C)|FD-Score:-3.16|P-value:7.90E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:YLR379W(YLR379W_d)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 689278
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADE8(YDR408C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AGX1(YFL030W)|FD-Score:6.19|P-value:3.04E-10||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:ARO1(YDR127W)|FD-Score:7.94|P-value:1.03E-15||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATP19(YOL077W-A)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:BEM3(YPL115C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BRR1(YPR057W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BTS1(YPL069C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC26(YFR036W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COQ5(YML110C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTF8(YHR191C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DON1(YDR273W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:EEB1(YPL095C)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EXO5(YBR163W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FAU1(YER183C)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FUS2(YMR232W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GPD1(YDL022W)|FD-Score:6.82|P-value:4.52E-12||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GTT1(YIR038C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:HSE1(YHL002W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IES1(YFL013C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:ISA1(YLL027W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:JNM1(YMR294W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAR5(YMR065W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:LDB17(YDL146W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LEA1(YPL213W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LRO1(YNR008W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MAM33(YIL070C)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MGA1(YGR249W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MKK1(YOR231W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MRPL4(YLR439W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSM1(YGR171C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:OCA1(YNL099C)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:ORM2(YLR350W)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OSW1(YOR255W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:OXA1(YER154W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PBY1(YBR094W)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress Gene:PCP1(YGR101W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PEP3(YLR148W)|FD-Score:7.13|P-value:5.12E-13||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET112(YBL080C)|FD-Score:-5.4|P-value:3.34E-8||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX32(YBR168W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFK1(YGR240C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PPT2(YPL148C)|FD-Score:4.49|P-value:3.55E-6||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRE9(YGR135W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PYC2(YBR218C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAD59(YDL059C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RAM1(YDL090C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RIF2(YLR453C)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL23B(YER117W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS19A(YOL121C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SWF1(YDR126W)|FD-Score:5.69|P-value:6.26E-9||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TRP3(YKL211C)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP5(YGL026C)|FD-Score:5.01|P-value:2.68E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.16|P-value:2.70E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP6(YFR010W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UFO1(YML088W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VBA3(YCL069W)|FD-Score:4.51|P-value:3.30E-6||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VHC1(YBR235W_p)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VPS17(YOR132W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YCL049C(YCL049C_p)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR006C(YCR006C_d)|FD-Score:4.31|P-value:8.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR051W(YCR051W_p)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR514C(YDR514C)|FD-Score:4.89|P-value:5.10E-7||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YGR164W(YGR164W_d)|FD-Score:-4.63|P-value:1.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR033W(YHR033W_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication Gene:YHR210C(YHR210C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL060W(YIL060W_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL161W(YIL161W_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YMR010W(YMR010W_p)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR306C-A(YMR306C-A_d)|FD-Score:-3.8|P-value:7.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR139C(YOR139C_d)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL261C(YPL261C_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR123C(YPR123C_d)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YVH1(YIR026C)|FD-Score:-5.1|P-value:1.70E-7||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ACO1(YLR304C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADE8(YDR408C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AGX1(YFL030W)|FD-Score:6.19|P-value:3.04E-10||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:ARO1(YDR127W)|FD-Score:7.94|P-value:1.03E-15||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATP19(YOL077W-A)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:BEM3(YPL115C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BRR1(YPR057W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BTS1(YPL069C)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC26(YFR036W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COQ5(YML110C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTF8(YHR191C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DON1(YDR273W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:EEB1(YPL095C)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EXO5(YBR163W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FAU1(YER183C)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FUS2(YMR232W)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GPD1(YDL022W)|FD-Score:6.82|P-value:4.52E-12||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GTT1(YIR038C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:HSE1(YHL002W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IES1(YFL013C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:ISA1(YLL027W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:JNM1(YMR294W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAR5(YMR065W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:LDB17(YDL146W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LEA1(YPL213W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LRO1(YNR008W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MAM33(YIL070C)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MGA1(YGR249W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MKK1(YOR231W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MRPL4(YLR439W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSM1(YGR171C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:OCA1(YNL099C)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:ORM2(YLR350W)|FD-Score:-3.77|P-value:8.31E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OSW1(YOR255W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:OXA1(YER154W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PBY1(YBR094W)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress Gene:PCP1(YGR101W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PEP3(YLR148W)|FD-Score:7.13|P-value:5.12E-13||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET112(YBL080C)|FD-Score:-5.4|P-value:3.34E-8||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PEX32(YBR168W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFK1(YGR240C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PPT2(YPL148C)|FD-Score:4.49|P-value:3.55E-6||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRE9(YGR135W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PYC2(YBR218C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAD59(YDL059C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RAM1(YDL090C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RIF2(YLR453C)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL23B(YER117W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS19A(YOL121C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SWF1(YDR126W)|FD-Score:5.69|P-value:6.26E-9||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TRP3(YKL211C)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP5(YGL026C)|FD-Score:5.01|P-value:2.68E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:9.16|P-value:2.70E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP6(YFR010W)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UFO1(YML088W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VBA3(YCL069W)|FD-Score:4.51|P-value:3.30E-6||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VHC1(YBR235W_p)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VPS17(YOR132W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YCL049C(YCL049C_p)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR006C(YCR006C_d)|FD-Score:4.31|P-value:8.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR051W(YCR051W_p)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR514C(YDR514C)|FD-Score:4.89|P-value:5.10E-7||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YGR164W(YGR164W_d)|FD-Score:-4.63|P-value:1.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR033W(YHR033W_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication Gene:YHR210C(YHR210C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL060W(YIL060W_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL161W(YIL161W_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YMR010W(YMR010W_p)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR306C-A(YMR306C-A_d)|FD-Score:-3.8|P-value:7.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR139C(YOR139C_d)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL261C(YPL261C_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR123C(YPR123C_d)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YVH1(YIR026C)|FD-Score:-5.1|P-value:1.70E-7||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C5.951.32E-90.56RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR319W5.773.89E-90.56HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YMR268C5.511.75E-80.56PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YBR004C5.373.93E-80.56GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YMR113W4.817.49E-70.15FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YFR002W4.661.60E-60.04NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YIL144W4.621.95E-60.09TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YMR049C4.523.02E-60.16ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YHR170W4.376.34E-60.25NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDR460W4.111.96E-50.01TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YDL152W_d4.102.07E-50.27YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YGR009C3.836.36E-50.16SEC9t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog
YDL030W3.671.20E-40.01PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YHR069C3.661.24E-40.18RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YLR379W_d3.482.49E-40.06YLR379W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A9.162.70E-20TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR127W7.941.03E-15ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YLR148W7.135.12E-13PEP3Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
YDL022W6.824.52E-12GPD1NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
YFL030W6.193.04E-10AGX1Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases
YDR126W5.696.26E-9SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YGR171C5.111.59E-7MSM1Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p
YBR094W5.091.77E-7PBY1Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress
YGL026C5.012.68E-7TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YDR514C4.895.10E-7YDR514CProtein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication
YKL211C4.562.58E-6TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YCL069W4.513.30E-6VBA3Permease of basic amino acids in the vacuolar membrane
YFR036W4.503.44E-6CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YPL148C4.493.55E-6PPT2Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YCR006C_d4.318.10E-6YCR006C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2086
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2571.19E-89SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0925926TSC3-RPN4
0.2531.13E-86SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0535714TSC3-RPN4
0.2351.06E-74SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.390244TSC3-RPN4
0.2273.78E-70SGTC_1726st035556 14.6 μMTimTec (Natural product derivative library)59194390.0961538
0.2221.32E-66SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.046875
0.2161.55E-63SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0588235
0.2131.60E-61SGTC_8600443-0016 25.1 μMChemDiv (Drug-like library)53379420.0961538
0.2093.05E-59SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.0952381TSC3-RPN4
0.1996.65E-54SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0508475TSC3-RPN4
0.1964.58E-52SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.405405TSC3-RPN4
0.1951.53E-51SGTC_21325317594 122.7 μMChembridge (Fragment library)7945770.192308SWF1 & branched chain AA biosynthesis
0.1951.65E-51SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0909091TSC3-RPN4
0.1905.05E-49SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.5TSC3-RPN4
0.1822.81E-45SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0375
0.1824.27E-45SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.170213TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_20785212016200 μM0.68751899708Chembridge (Fragment library)288.755362.1272560S ribosome export
SGTC_20735212005126 μM0.657143691789Chembridge (Fragment library)288.755362.12725
SGTC_20755212009200 μM0.657143780898Chembridge (Fragment library)272.3007631.66826
SGTC_20765212012200 μM0.657143604802Chembridge (Fragment library)255.298360.31226
SGTC_21115486399200 μM0.628571689674Chembridge (Fragment library)282.363462.40525
SGTC_20885278280127.39 μM0.605263791502Chembridge (Fragment library)284.336281.44726
SGTC_2074521200775.4 μM0.6758611Chembridge (Fragment library)272.3007631.66826
SGTC_20775212015200 μM0.6780900Chembridge (Fragment library)288.755362.12725
SGTC_21135491891107.03 μM0.571429789979Chembridge (Fragment library)268.336881.91925TSC3-RPN4
SGTC_2071523095139.92 μM0.5555561799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4