5276107

1-[(3-chloroanilino)methyl]pyrrolidine-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2087
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 144405
SMILES C1CC(=O)N(C1=O)CNC2=CC(=CC=C2)Cl
Standardized SMILES Clc1cccc(NCN2C(=O)CCC2=O)c1
Molecular weight 238.6702
ALogP 1.52
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.7
% growth inhibition (Hom. pool) 4.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 144405
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.15||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CEP3(YMR168C)|FD-Score:4.51|P-value:3.22E-6|Clearance:0.27||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CLF1(YLR117C)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:CRM1(YGR218W)|FD-Score:-3.25|P-value:5.78E-4|Clearance:0||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:ERG13(YML126C)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.07||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:EXO84(YBR102C)|FD-Score:-3.47|P-value:2.56E-4|Clearance:0||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:INO80(YGL150C)|FD-Score:3.14|P-value:8.58E-4|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:KAP95(YLR347C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.2||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:NAB3(YPL190C)|FD-Score:3.79|P-value:7.39E-5|Clearance:0.19||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:RHO1(YPR165W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.12||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RRP43(YCR035C)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.27||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:3.98|P-value:3.48E-5|Clearance:0.18||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:YBR124W(YBR124W_d)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.21||SGD DESC:Putative protein of unknown function Gene:YDL016C(YDL016C_d)|FD-Score:4.03|P-value:2.73E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YKL036C(YKL036C_d)|FD-Score:4.88|P-value:5.24E-7|Clearance:0.27||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:ATM1(YMR301C)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.15||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CEP3(YMR168C)|FD-Score:4.51|P-value:3.22E-6|Clearance:0.27||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CLF1(YLR117C)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:CRM1(YGR218W)|FD-Score:-3.25|P-value:5.78E-4|Clearance:0||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:ERG13(YML126C)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.07||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:EXO84(YBR102C)|FD-Score:-3.47|P-value:2.56E-4|Clearance:0||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:INO80(YGL150C)|FD-Score:3.14|P-value:8.58E-4|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:KAP95(YLR347C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.2||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:NAB3(YPL190C)|FD-Score:3.79|P-value:7.39E-5|Clearance:0.19||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:RHO1(YPR165W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.12||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RRP43(YCR035C)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.27||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:3.98|P-value:3.48E-5|Clearance:0.18||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:YBR124W(YBR124W_d)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.21||SGD DESC:Putative protein of unknown function Gene:YDL016C(YDL016C_d)|FD-Score:4.03|P-value:2.73E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YKL036C(YKL036C_d)|FD-Score:4.88|P-value:5.24E-7|Clearance:0.27||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 144405
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ARO80(YDR421W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:BUD26(YDR241W_d)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBS1(YDL069C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CLB1(YGR108W)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG1(YGL223C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRP1(YHR146W)|FD-Score:4.57|P-value:2.40E-6||SGD DESC:Protein that binds to cruciform DNA structures Gene:DAL2(YIR029W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DYN2(YDR424C)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:FLO8(YER109C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FPR1(YNL135C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GAP1(YKR039W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GLG1(YKR058W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:6.04|P-value:7.89E-10||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HVG1(YER039C)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:HXT3(YDR345C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:IGO2(YHR132W-A)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:KAR4(YCL055W)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KTI11(YBL071W-A)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MET10(YFR030W)|FD-Score:5.31|P-value:5.34E-8||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET17(YLR303W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET28(YIR017C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MHR1(YDR296W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRPL32(YCR003W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NCA2(YPR155C)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p Gene:NTH2(YBR001C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PER1(YCR044C)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX19(YDL065C)|FD-Score:4.84|P-value:6.49E-7||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PRR2(YDL214C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PRS1(YKL181W)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PUN1(YLR414C)|FD-Score:-3.96|P-value:3.79E-5||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:PUS7(YOR243C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD28(YDR030C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RAD57(YDR004W)|FD-Score:4.32|P-value:7.77E-6||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RNP1(YLL046C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL14A(YKL006W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL16A(YIL133C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL35A(YDL191W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS7A(YOR096W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SCJ1(YMR214W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SFA1(YDL168W)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SGM1(YJR134C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SNF7(YLR025W)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SRB5(YGR104C)|FD-Score:4.44|P-value:4.43E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRB8(YCR081W)|FD-Score:5.21|P-value:9.25E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRO7(YPR032W)|FD-Score:-5.08|P-value:1.84E-7||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TIP41(YPR040W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOP1(YOL006C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:UBX4(YMR067C)|FD-Score:-4.52|P-value:3.09E-6||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:URA8(YJR103W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VPS64(YDR200C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YAP5(YIR018W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBL070C(YBL070C_d)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL002C(YCL002C_p)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDL162C(YDL162C_d)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR514C(YDR514C)|FD-Score:5|P-value:2.83E-7||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YFR035C(YFR035C_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL081W(YGL081W_p)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YGR053C(YGR053C_p)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Putative protein of unknown function Gene:YGR210C(YGR210C_p)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR177W(YHR177W_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIR007W(YIR007W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YLR419W(YLR419W_p)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL203C(YNL203C_d)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL118C(YOL118C_d)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR200W(YOR200W_d)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPR027C(YPR027C_p)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Putative protein of unknown function Gene:ZIP1(YDR285W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:AAD3(YCR107W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ARO80(YDR421W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:BUD26(YDR241W_d)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CBS1(YDL069C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CLB1(YGR108W)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG1(YGL223C)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRP1(YHR146W)|FD-Score:4.57|P-value:2.40E-6||SGD DESC:Protein that binds to cruciform DNA structures Gene:DAL2(YIR029W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DYN2(YDR424C)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:FLO8(YER109C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FPR1(YNL135C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GAP1(YKR039W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GLG1(YKR058W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:6.04|P-value:7.89E-10||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HVG1(YER039C)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:HXT3(YDR345C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:IGO2(YHR132W-A)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:KAR4(YCL055W)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KTI11(YBL071W-A)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MET10(YFR030W)|FD-Score:5.31|P-value:5.34E-8||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET17(YLR303W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET28(YIR017C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MHR1(YDR296W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRPL32(YCR003W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:NCA2(YPR155C)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p Gene:NTH2(YBR001C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PER1(YCR044C)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX19(YDL065C)|FD-Score:4.84|P-value:6.49E-7||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PRR2(YDL214C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PRS1(YKL181W)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PUN1(YLR414C)|FD-Score:-3.96|P-value:3.79E-5||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:PUS7(YOR243C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD28(YDR030C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RAD57(YDR004W)|FD-Score:4.32|P-value:7.77E-6||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RNP1(YLL046C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL14A(YKL006W)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL16A(YIL133C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL35A(YDL191W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPP1B(YDL130W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS7A(YOR096W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SCJ1(YMR214W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SFA1(YDL168W)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SGM1(YJR134C)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SNF7(YLR025W)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SRB5(YGR104C)|FD-Score:4.44|P-value:4.43E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRB8(YCR081W)|FD-Score:5.21|P-value:9.25E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SRO7(YPR032W)|FD-Score:-5.08|P-value:1.84E-7||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TIP41(YPR040W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOP1(YOL006C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:UBX4(YMR067C)|FD-Score:-4.52|P-value:3.09E-6||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:URA8(YJR103W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VPS64(YDR200C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YAP5(YIR018W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBL070C(YBL070C_d)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL002C(YCL002C_p)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDL162C(YDL162C_d)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR514C(YDR514C)|FD-Score:5|P-value:2.83E-7||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YFR035C(YFR035C_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL081W(YGL081W_p)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YGR053C(YGR053C_p)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Putative protein of unknown function Gene:YGR210C(YGR210C_p)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR177W(YHR177W_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIR007W(YIR007W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YLR419W(YLR419W_p)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL203C(YNL203C_d)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL118C(YOL118C_d)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR200W(YOR200W_d)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPR027C(YPR027C_p)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Putative protein of unknown function Gene:ZIP1(YDR285W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL036C_d4.885.24E-70.27YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YCR035C4.731.10E-60.27RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YMR168C4.513.22E-60.27CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YBR124W_d4.241.11E-50.21YBR124W_dPutative protein of unknown function
YDL016C_d4.032.73E-50.06YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YGR095C3.983.48E-50.18RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YPL190C3.797.39E-50.19NAB3RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
YPR165W3.601.58E-40.12RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YLR347C3.482.53E-40.20KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
YMR301C3.285.14E-40.15ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YGL150C3.148.58E-40.00INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YML126C3.138.63E-40.07ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YBL084C3.070.001080.05CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YDL208W3.020.001270.08NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
YGL065C2.940.001630.04ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR251W6.047.89E-10GTO3Omega class glutathione transferase; putative cytosolic localization
YPR040W5.601.07E-8TIP41Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress
YFR030W5.315.34E-8MET10Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide
YCR081W5.219.25E-8SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YDR514C5.002.83E-7YDR514CProtein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication
YDL065C4.846.49E-7PEX19Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning
YOR243C4.721.16E-6PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YLR025W4.721.17E-6SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YDR296W4.651.69E-6MHR1Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
YPR027C_p4.602.13E-6YPR027C_pPutative protein of unknown function
YHR146W4.572.40E-6CRP1Protein that binds to cruciform DNA structures
YGR104C4.444.43E-6SRB5Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
YCR044C4.337.53E-6PER1Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
YDR004W4.327.77E-6RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YCR003W4.141.75E-5MRPL32Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2087
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0814.98E-10SGTC_24345670830 169.9 μMMiscellaneous15498300.15
0.0731.64E-8SGTC_20985376439 882.5 nMChembridge (Fragment library)28441160.116667
0.0722.82E-8SGTC_20945332512 191.7 μMChembridge (Fragment library)7557740.169811
0.0663.71E-7SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.15942cell wall
0.0649.77E-7SGTC_1082camptothecin 424.9 nMTimTec (Natural product library)25380.131579DNA damage response
0.0631.46E-6SGTC_219mitoxanthrone 164.4 μMMiscellaneous42120.148148DNA intercalators
0.0603.66E-6SGTC_1684st024538 14.6 μMTimTec (Natural product derivative library)67328590.106061
0.0588.52E-6SGTC_478N-arachidonylglycine 10.0 μMICCB bioactive library52833890.0491803
0.0542.85E-5SGTC_8500388-0143 75.9 μMChemDiv (Drug-like library)672730.122807
0.0535.15E-5SGTC_2762leflunomide 42.1 μMMiscellaneous38990.0983607
0.0525.50E-5SGTC_22907904846 200.0 μMChembridge (Fragment library)29621540.0819672
0.0526.00E-5SGTC_276colchicine 750.0 μMMiscellaneous28330.108108
0.0517.81E-5SGTC_33319141060 2.0 μMChembridge (Drug-like library)49065670.142857cell wall
0.0511.01E-4SGTC_257daunorubicin 18.5 μMMiscellaneous303230.0869565anthracycline transcription coupled DNA repair
0.0501.28E-4SGTC_30929117248 49.5 μMChembridge (Drug-like library)171418570.305085cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21215257820149.64 μM0.647059791256Chembridge (Fragment library)204.225180.85313
SGTC_21005377183190 μM0.3752844202Chembridge (Fragment library)250.316762.6631460S ribosome export
SGTC_21925915882198.49 μM0.3695652876149Chembridge (Fragment library)268.310441.5513RNA processing & uracil transport
SGTC_1964st07691461 μM0.35714324208661TimTec (Natural product derivative library)327.761663.82714RPP1 & pyrimidine depletion
SGTC_21015377231179.23 μM0.3541672844213Chembridge (Fragment library)256.708662.35514
SGTC_2961908329032.55 μM0.34693917741490Chembridge (Drug-like library)295.784463.47513
SGTC_20995377090177.37 μM0.3404262243829Chembridge (Fragment library)240.2540631.89615
SGTC_2392N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide200 μM0.34803109Chembridge (Fragment library)248.665061.08522
SGTC_2892904735858.44 μM0.3333336466771Chembridge (Drug-like library)379.861122.414
SGTC_2896905265671.43 μM0.3333336469436Chembridge (Drug-like library)369.887763.62913endomembrane recycling