4-phenylthiadiazole

4-phenylthiadiazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2091
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 270010
SMILES C1=CC=C(C=C1)C2=CSN=N2
Standardized SMILES c1ccc(cc1)c2csnn2
Molecular weight 162.2116
ALogP 2.32
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.6
% growth inhibition (Hom. pool) 5.8


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 270010
Download HIP data (tab-delimited text)  (excel)
Gene:BBP1(YPL255W)|FD-Score:5.52|P-value:1.66E-8|Clearance:1.84||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:BRF1(YGR246C)|FD-Score:3.2|P-value:6.77E-4|Clearance:0.01||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:BRL1(YHR036W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.37||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:DHR2(YKL078W)|FD-Score:-3.11|P-value:9.31E-4|Clearance:0||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:MCM3(YEL032W)|FD-Score:3.19|P-value:7.04E-4|Clearance:0.07||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:SPP381(YBR152W)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.11||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TFC8(YPL007C)|FD-Score:-3.9|P-value:4.81E-5|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:YDL016C(YDL016C_d)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:BBP1(YPL255W)|FD-Score:5.52|P-value:1.66E-8|Clearance:1.84||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:BRF1(YGR246C)|FD-Score:3.2|P-value:6.77E-4|Clearance:0.01||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:BRL1(YHR036W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.37||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:DHR2(YKL078W)|FD-Score:-3.11|P-value:9.31E-4|Clearance:0||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:MCM3(YEL032W)|FD-Score:3.19|P-value:7.04E-4|Clearance:0.07||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:SPP381(YBR152W)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.11||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TFC8(YPL007C)|FD-Score:-3.9|P-value:4.81E-5|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:YDL016C(YDL016C_d)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 270010
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AKR1(YDR264C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALY1(YKR021W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:ATX2(YOR079C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation Gene:BUL1(YMR275C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAT2(YML042W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CHS7(YHR142W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG8(YML071C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX19(YLL018C-A)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DAS2(YDR020C_p)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases Gene:DER1(YBR201W)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p Gene:END3(YNL084C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:EST3(YIL009C-A)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FET4(YMR319C)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GTT2(YLL060C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HPR1(YDR138W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:KKQ8(YKL168C)|FD-Score:5.96|P-value:1.28E-9||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LIP2(YLR239C)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAK10(YEL053C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MCK1(YNL307C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MET17(YLR303W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MKK1(YOR231W)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MRPL4(YLR439W)|FD-Score:5.24|P-value:8.15E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL8(YJL063C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OST6(YML019W)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PEP3(YLR148W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:RMR1(YGL250W)|FD-Score:-3.79|P-value:7.46E-5||SGD DESC:Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus Gene:RPL14A(YKL006W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RTR2(YDR066C_p)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SNF11(YDR073W)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SPC2(YML055W)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:STO1(YMR125W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWA2(YDR320C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:THP3(YPR045C)|FD-Score:3.95|P-value:3.99E-5||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TLG2(YOL018C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP2(YER090W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:VBA2(YBR293W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS51(YKR020W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS62(YGR141W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:YBR284W(YBR284W_p)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR051W(YCR051W_p)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR199W(YDR199W_d)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YEL067C(YEL067C_p)|FD-Score:7.4|P-value:6.71E-14||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YER097W(YER097W_d)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR168C(YGR168C_p)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YKL023W(YKL023W_p)|FD-Score:5.24|P-value:8.14E-8||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR005C(YKR005C_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function Gene:YKR040C(YKR040C_d)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YLR280C(YLR280C_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR253C(YMR253C_p)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNR040W(YNR040W_p)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR1(YGR281W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:ADO1(YJR105W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AKR1(YDR264C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALY1(YKR021W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:ATX2(YOR079C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation Gene:BUL1(YMR275C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAT2(YML042W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CHS7(YHR142W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG8(YML071C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX19(YLL018C-A)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DAS2(YDR020C_p)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases Gene:DER1(YBR201W)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p Gene:END3(YNL084C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:EST3(YIL009C-A)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FET4(YMR319C)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GTT2(YLL060C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HPR1(YDR138W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:KKQ8(YKL168C)|FD-Score:5.96|P-value:1.28E-9||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LIP2(YLR239C)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAK10(YEL053C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MCK1(YNL307C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MET17(YLR303W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MKK1(YOR231W)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MRPL4(YLR439W)|FD-Score:5.24|P-value:8.15E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL8(YJL063C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OST6(YML019W)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PEP3(YLR148W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:RMR1(YGL250W)|FD-Score:-3.79|P-value:7.46E-5||SGD DESC:Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus Gene:RPL14A(YKL006W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RTR2(YDR066C_p)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SNF11(YDR073W)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SPC2(YML055W)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:STO1(YMR125W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWA2(YDR320C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:THP3(YPR045C)|FD-Score:3.95|P-value:3.99E-5||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TLG2(YOL018C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP2(YER090W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:VBA2(YBR293W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS51(YKR020W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS62(YGR141W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:YBR284W(YBR284W_p)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR051W(YCR051W_p)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDR199W(YDR199W_d)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YEL067C(YEL067C_p)|FD-Score:7.4|P-value:6.71E-14||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YER097W(YER097W_d)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR168C(YGR168C_p)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YKL023W(YKL023W_p)|FD-Score:5.24|P-value:8.14E-8||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR005C(YKR005C_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function Gene:YKR040C(YKR040C_d)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YLR280C(YLR280C_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR253C(YMR253C_p)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNR040W(YNR040W_p)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR1(YGR281W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR)

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL255W5.521.66E-81.84BBP1Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p
YHR036W3.681.15E-40.37BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YBR152W3.314.65E-40.11SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YGR246C3.206.77E-40.01BRF1TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
YEL032W3.197.04E-40.07MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YDL016C_d3.128.90E-40.09YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YDR356W3.040.001190.00SPC110Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
YGR186W3.030.001210.12TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YOL010W2.910.001790.06RCL1Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
YGR191W2.850.002180.01HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YFR050C2.840.002240.01PRE4Beta 7 subunit of the 20S proteasome
YJL015C_d2.840.002280.09YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YPL235W2.750.003000.03RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YKR063C2.720.003300.07LAS1Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
YMR220W2.650.004020.04ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL067C_p7.406.71E-14YEL067C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YKL168C5.961.28E-9KKQ8Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication
YKL023W_p5.248.14E-8YKL023W_pPutative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YLR439W5.248.15E-8MRPL4Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
YNR040W_p4.963.51E-7YNR040W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YJR105W4.711.25E-6ADO1Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
YMR125W4.641.75E-6STO1Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
YML019W4.356.85E-6OST6Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
YGR168C_p4.141.75E-5YGR168C_pPutative protein of unknown function; YGR168C is not an essential gene
YKR020W4.102.03E-5VPS51Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p
YDR199W_d4.082.29E-5YDR199W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
YJL063C3.963.75E-5MRPL8Mitochondrial ribosomal protein of the large subunit
YPR045C3.953.99E-5THP3Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
YNL084C3.944.10E-5END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YNL307C3.934.23E-5MCK1Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family

GO enrichment analysis for SGTC_2091
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0897.15E-12SGTC_30539095903 49.5 μMChembridge (Drug-like library)171740180.0677966Golgi
0.0831.57E-10SGTC_1735st034310 56.8 μMTimTec (Natural product derivative library)8711530.0576923Golgi
0.0815.82E-10SGTC_7551995-0008 37.3 μMChemDiv (Drug-like library)6895330.0869565
0.0731.63E-8SGTC_22027226349 200.0 μMChembridge (Fragment library)8443340.08
0.0706.30E-8SGTC_29005328528 85.3 μMChembridge (Drug-like library)7953590.0769231Golgi
0.0691.04E-7SGTC_1235benzylparaben 21.5 μMChemDiv (Drug-like library)71800.139535
0.0691.04E-7SGTC_15666,7-dimethoxy-4-ethylcoumarin 85.4 μMTimTec (Pure natural product library)6887610.04
0.0672.35E-7SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.113208
0.0672.81E-7SGTC_1990st072050 59.8 μMTimTec (Natural product derivative library)172339480.0338983
0.0663.41E-7SGTC_2552juarezic acid 87.0 μMMicrosource (Natural product library)15495120.153846TSC3-RPN4
0.0663.77E-7SGTC_18903',4',7',8'-tetrahydroxyflavone 14.9 μMTimTec (Natural product derivative library)6887980.08
0.0664.52E-7SGTC_10410206-0046 107.0 μMChemDiv (Drug-like library)504940.0961538
0.0655.91E-7SGTC_8211114-0266 29.2 μMChemDiv (Drug-like library)31232350.12
0.0647.60E-7SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.190476
0.0631.32E-6SGTC_1857st056007 79.3 μMTimTec (Natural product derivative library)6198340.14

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8800833-048787.2 μM0.39393976075ChemDiv (Drug-like library)252.334183.54513mitochondrial processes
SGTC_1524469-0003551.23 μM0.325697280ChemDiv (Drug-like library)268.333584.08224
SGTC_6281636-025316 μM0.3254456143ChemDiv (Drug-like library)331.230245.07213sphingolipid biosynthesis & PDR1
SGTC_20084012253172 μM0.314286603940Chembridge (Fragment library)185.225142.05513
SGTC_13521496-099199 μM0.3076923000039ChemDiv (Drug-like library)215.274261.98603tubulin folding & SWR complex
SGTC_9661300-036030.2 μM0.28947470588ChemDiv (Drug-like library)310.351964.37504heme requiring
SGTC_13501495-0454146 μM0.2888891069057ChemDiv (Drug-like library)350.843043.92615
SGTC_23829035899200 μM0.2888896462679Chembridge (Fragment library)288.368041.9431460S ribosome export
SGTC_1069diphenylcyclopropenone8.49 μM0.28571465057NIH Clinical Collection206.23933.30101
SGTC_230MPP+141.67 μM0.26666739484Miscellaneous170.230382.95300