5468907

3-amino-4-(dimethylamino)thieno[2,3-b]pyridine-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2096
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 759785
SMILES CN(C)C1=C2C(=C(SC2=NC=C1)C(=O)N)N
Standardized SMILES CN(C)c1ccnc2sc(C(=O)N)c(N)c12
Molecular weight 236.2935
ALogP 0.45
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.14
% growth inhibition (Hom. pool) 3.8


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 759785
Download HIP data (tab-delimited text)  (excel)
Gene:BBP1(YPL255W)|FD-Score:-3.43|P-value:3.00E-4|Clearance:0||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:DML1(YMR211W)|FD-Score:6.31|P-value:1.42E-10|Clearance:2.38||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:OST1(YJL002C)|FD-Score:3.29|P-value:4.94E-4|Clearance:0.02||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RAD3(YER171W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.03||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RPT1(YKL145W)|FD-Score:3.92|P-value:4.34E-5|Clearance:0.2||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:TAO3(YIL129C)|FD-Score:3.24|P-value:5.90E-4|Clearance:0.28||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:YGR114C(YGR114C_d)|FD-Score:3.72|P-value:9.90E-5|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:BBP1(YPL255W)|FD-Score:-3.43|P-value:3.00E-4|Clearance:0||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:DML1(YMR211W)|FD-Score:6.31|P-value:1.42E-10|Clearance:2.38||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:OST1(YJL002C)|FD-Score:3.29|P-value:4.94E-4|Clearance:0.02||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RAD3(YER171W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.03||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RPT1(YKL145W)|FD-Score:3.92|P-value:4.34E-5|Clearance:0.2||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:TAO3(YIL129C)|FD-Score:3.24|P-value:5.90E-4|Clearance:0.28||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:YGR114C(YGR114C_d)|FD-Score:3.72|P-value:9.90E-5|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 759785
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:-6.25|P-value:2.12E-10||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AKR1(YDR264C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARI1(YGL157W)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:BST1(YFL025C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:ECM4(YKR076W)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:FLO8(YER109C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:HBS1(YKR084C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:LPX1(YOR084W)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction Gene:MAE1(YKL029C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MET28(YIR017C)|FD-Score:-4.65|P-value:1.63E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MRP10(YDL045W-A)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPL4(YLR439W)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSI1(YBR195C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MTC6(YHR151C)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NUP188(YML103C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Gene:PET111(YMR257C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET112(YBL080C)|FD-Score:-4.45|P-value:4.33E-6||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET309(YLR067C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PEX17(YNL214W)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PGD1(YGL025C)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:RAD26(YJR035W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RCY1(YJL204C)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM21(YNL294C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPS4B(YHR203C)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SKY1(YMR216C)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPC2(YML055W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPT8(YLR055C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:STO1(YMR125W)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUV3(YPL029W)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TRE1(YPL176C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:VIP1(YLR410W)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VMA13(YPR036W)|FD-Score:-3.83|P-value:6.32E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS1(YKR001C)|FD-Score:-4.35|P-value:6.66E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VTH1(YIL173W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YCR085W(YCR085W_d)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL241W(YDL241W_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YFR018C(YFR018C_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function Gene:YGL242C(YGL242C_p)|FD-Score:-3.24|P-value:5.96E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YML100W-A(YML100W-A_p)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOR082C(YOR082C_d)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPK1(YKL126W)|FD-Score:3.74|P-value:9.25E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ADE8(YDR408C)|FD-Score:-6.25|P-value:2.12E-10||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AKR1(YDR264C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARI1(YGL157W)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:BST1(YFL025C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:ECM4(YKR076W)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:FLO8(YER109C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:HBS1(YKR084C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:LPX1(YOR084W)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction Gene:MAE1(YKL029C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MET28(YIR017C)|FD-Score:-4.65|P-value:1.63E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MRP10(YDL045W-A)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPL4(YLR439W)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSI1(YBR195C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MTC6(YHR151C)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NUP188(YML103C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 Gene:PET111(YMR257C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET112(YBL080C)|FD-Score:-4.45|P-value:4.33E-6||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET309(YLR067C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PEX17(YNL214W)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PGD1(YGL025C)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:RAD26(YJR035W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RCY1(YJL204C)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM21(YNL294C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPS4B(YHR203C)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SKY1(YMR216C)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPC2(YML055W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPT8(YLR055C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:STO1(YMR125W)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SUV3(YPL029W)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TRE1(YPL176C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:VIP1(YLR410W)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VMA13(YPR036W)|FD-Score:-3.83|P-value:6.32E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS1(YKR001C)|FD-Score:-4.35|P-value:6.66E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VTH1(YIL173W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YCR085W(YCR085W_d)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL241W(YDL241W_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YFR018C(YFR018C_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function Gene:YGL242C(YGL242C_p)|FD-Score:-3.24|P-value:5.96E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YML100W-A(YML100W-A_p)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOR082C(YOR082C_d)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPK1(YKL126W)|FD-Score:3.74|P-value:9.25E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR211W6.311.42E-102.38DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YKL145W3.924.34E-50.20RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YGR114C_d3.729.90E-50.43YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YJL002C3.294.94E-40.02OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YER171W3.285.24E-40.03RAD35' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
YIL129C3.245.90E-40.28TAO3Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
YLR008C2.960.001520.09PAM18Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
YFL009W2.880.002010.05CDC4F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
YNL102W2.820.002390.02POL1Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
YJR016C2.800.002580.09ILV3Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
YHR007C2.710.003370.03ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YGL093W2.680.003683.83E-4SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YIL046W2.680.003690.02MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YOR159C2.660.003930.05SME1Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
YDR454C2.610.004520.00GUK1Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL176C5.191.08E-7TRE1Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication
YOR084W5.181.09E-7LPX1Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction
YBR195C4.211.29E-5MSI1Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase
YDR159W4.102.07E-5SAC3Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
YNL214W4.022.86E-5PEX17Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis
YHR203C3.934.20E-5RPS4BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication
YCR085W_d3.934.22E-5YCR085W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YJL204C3.914.55E-5RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YGL157W3.826.63E-5ARI1NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
YKL126W3.749.25E-5YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR410W3.739.43E-5VIP1Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1
YHR151C3.739.74E-5MTC6Protein of unknown function; mtc6 is synthetically sick with cdc13-1
YKR076W3.729.92E-5ECM4Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YFR018C_p3.621.45E-4YFR018C_pPutative protein of unknown function
YML103C3.601.57E-4NUP188Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188

GO enrichment analysis for SGTC_2096
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0985.70E-14SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.0967742
0.0978.64E-14SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.1
0.0936.52E-13SGTC_266formosanin C 406.0 nMMiscellaneous735970.025641sphingolipid biosynthesis & PDR1
0.0912.61E-12SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.0300752
0.0903.30E-12SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.0405405endomembrane recycling
0.0881.36E-11SGTC_30859117376 49.5 μMChembridge (Drug-like library)164803490.0933333
0.0857.48E-11SGTC_4260988-0037 14.6 μMChemDiv (Drug-like library)53830080.0588235ERAD & cell cycle
0.0822.94E-10SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)31320570.0649351DNA damage response
0.0809.39E-10SGTC_1811447-1644 8.1 μMChemDiv (Drug-like library)5346000.037037endomembrane recycling
0.0791.45E-9SGTC_23809035439 200.0 μMChembridge (Fragment library)64625750.0909091endomembrane recycling
0.0782.30E-9SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0963855
0.0764.04E-9SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.057971fatty acid desaturase (OLE1)
0.0756.91E-9SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.0606061fatty acid desaturase (OLE1)
0.0758.05E-9SGTC_2732terfenadine 20.0 μMMiscellaneous54050.0526316ERG2
0.0759.39E-9SGTC_2567aureobasidin a 60.0 nMMiscellaneous99634300.0438596

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3096911746849.47 μM0.33333341316311Chembridge (Drug-like library)247.316121.1614
SGTC_3128912769149.47 μM0.341316314Chembridge (Drug-like library)338.426722.32815
SGTC_14853909-9022254 μM0.271186714516ChemDiv (Drug-like library)293.384563.49206RPP1 & pyrimidine depletion
SGTC_22067254556146.97 μM0.2456144398905Chembridge (Fragment library)275.326220.8842460S ribosome export
SGTC_7521896-0090130 μM0.241379772567ChemDiv (Drug-like library)294.147182.04913TSC3-RPN4
SGTC_2159565861173.75 μM0.241379793844Chembridge (Fragment library)289.35281.9234
SGTC_8324083-0019164 μM0.2328771990758ChemDiv (Drug-like library)492.494013.664310translation
SGTC_2042p-Dimethylaminoacetophenone200 μM0.22222275037Chembridge (Fragment library)163.216321.73202
SGTC_23186029776164 μM0.222222818325Chembridge (Fragment library)204.268241.65513
SGTC_23017769476200 μM0.216667977820Chembridge (Fragment library)253.343841.85915