5377090

3-[(4-fluoroanilino)methyl]-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2099
Screen concentration 177.4 μM
Source Chembridge (Fragment library)
PubChem CID 2243829
SMILES C1C(=O)N(C(=O)S1)CNC2=CC=C(C=C2)F
Standardized SMILES Fc1ccc(NCN2C(=O)CSC2=O)cc1
Molecular weight 240.2541
ALogP 1.9
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.27
% growth inhibition (Hom. pool) 2.55


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2243829
Download HIP data (tab-delimited text)  (excel)
Gene:HYM1(YKL189W)|FD-Score:-3.89|P-value:5.02E-5|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:ILV5(YLR355C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.13||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:KIN28(YDL108W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.5||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:KRE9(YJL174W)|FD-Score:-5.29|P-value:6.20E-8|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:MCM10(YIL150C)|FD-Score:-3.81|P-value:6.84E-5|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MCM5(YLR274W)|FD-Score:-3.86|P-value:5.57E-5|Clearance:0||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:PKC1(YBL105C)|FD-Score:3.29|P-value:4.97E-4|Clearance:0.13||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRI2(YKL045W)|FD-Score:-3.24|P-value:5.97E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RET2(YFR051C)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RFT1(YBL020W)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.14||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPP0(YLR340W)|FD-Score:8.63|P-value:3.08E-18|Clearance:3.72||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC10(YLR166C)|FD-Score:-3.98|P-value:3.42E-5|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SPC110(YDR356W)|FD-Score:-3.27|P-value:5.36E-4|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:SPC42(YKL042W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.13||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAD2(YJL035C)|FD-Score:-3.75|P-value:8.80E-5|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TFB2(YPL122C)|FD-Score:4.91|P-value:4.58E-7|Clearance:0.31||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TIM54(YJL054W)|FD-Score:4.6|P-value:2.14E-6|Clearance:0.47||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:YBR190W(YBR190W_d)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YGR115C(YGR115C_d)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YLR198C(YLR198C_d)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YRA1(YDR381W)|FD-Score:-3.37|P-value:3.74E-4|Clearance:0||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:HYM1(YKL189W)|FD-Score:-3.89|P-value:5.02E-5|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:ILV5(YLR355C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.13||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:KIN28(YDL108W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.5||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:KRE9(YJL174W)|FD-Score:-5.29|P-value:6.20E-8|Clearance:0||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:MCM10(YIL150C)|FD-Score:-3.81|P-value:6.84E-5|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MCM5(YLR274W)|FD-Score:-3.86|P-value:5.57E-5|Clearance:0||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:PKC1(YBL105C)|FD-Score:3.29|P-value:4.97E-4|Clearance:0.13||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRI2(YKL045W)|FD-Score:-3.24|P-value:5.97E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RET2(YFR051C)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RFT1(YBL020W)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.14||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPP0(YLR340W)|FD-Score:8.63|P-value:3.08E-18|Clearance:3.72||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SEC10(YLR166C)|FD-Score:-3.98|P-value:3.42E-5|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SPC110(YDR356W)|FD-Score:-3.27|P-value:5.36E-4|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:SPC42(YKL042W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.13||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAD2(YJL035C)|FD-Score:-3.75|P-value:8.80E-5|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TFB2(YPL122C)|FD-Score:4.91|P-value:4.58E-7|Clearance:0.31||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TIM54(YJL054W)|FD-Score:4.6|P-value:2.14E-6|Clearance:0.47||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:YBR190W(YBR190W_d)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YGR115C(YGR115C_d)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YLR198C(YLR198C_d)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YRA1(YDR381W)|FD-Score:-3.37|P-value:3.74E-4|Clearance:0||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2243829
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:4.3|P-value:8.45E-6||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADR1(YDR216W)|FD-Score:4.34|P-value:7.08E-6||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ADY4(YLR227C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:AGA2(YGL032C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIP1(YMR092C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ALG5(YPL227C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:AMD1(YML035C)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:APE3(YBR286W)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APL1(YJR005W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:APN1(YKL114C)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARE2(YNR019W)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ATO3(YDR384C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:ATP3(YBR039W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:COX17(YLL009C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DBP1(YPL119C)|FD-Score:-4.42|P-value:4.88E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DBP7(YKR024C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DCN1(YLR128W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DOA1(YKL213C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DOS2(YDR068W)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERG3(YLR056W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERV25(YML012W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FAR3(YMR052W)|FD-Score:4.92|P-value:4.25E-7||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FMP48(YGR052W_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FPR1(YNL135C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GAC1(YOR178C)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GLC3(YEL011W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GLO3(YER122C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GTT3(YEL017W)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HMT1(YBR034C)|FD-Score:4.98|P-value:3.15E-7||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:KAP123(YER110C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KTR2(YKR061W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LST7(YGR057C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MDS3(YGL197W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MEI4(YER044C-A)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MMM1(YLL006W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MPP6(YNR024W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPS28(YDR337W)|FD-Score:5.95|P-value:1.36E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NOT5(YPR072W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OCA1(YNL099C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OCA4(YCR095C_p)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PEP7(YDR323C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PPT2(YPL148C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD18(YCR066W)|FD-Score:6.89|P-value:2.74E-12||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RED1(YLR263W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RPL14A(YKL006W)|FD-Score:7.46|P-value:4.47E-14||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL4A(YBR031W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SDH4(YDR178W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SFA1(YDL168W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHU2(YDR078C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SKG1(YKR100C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SKI2(YLR398C)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SKI3(YPR189W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SRL3(YKR091W)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:TGL3(YMR313C)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:UBP6(YFR010W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UPS3(YDR185C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDL032W(YDL032W_d)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YEL057C(YEL057C_p)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER077C(YER077C_p)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGL034C(YGL034C_d)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR067C(YGR067C_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p Gene:YGR146C-A(YGR146C-A_p)|FD-Score:3.89|P-value:4.91E-5||SGD DESC:Putative protein of unknown function Gene:YHR202W(YHR202W_p)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YKL071W(YKL071W_p)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR011C(YKR011C_p)|FD-Score:5.64|P-value:8.26E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR184W(YLR184W_d)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Putative protein of unknown function Gene:YMR057C(YMR057C_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YOL019W(YOL019W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOL098C(YOL098C_p)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Putative metalloprotease Gene:YPR053C(YPR053C_d)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:ADE8(YDR408C)|FD-Score:4.3|P-value:8.45E-6||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADR1(YDR216W)|FD-Score:4.34|P-value:7.08E-6||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ADY4(YLR227C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:AGA2(YGL032C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIP1(YMR092C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ALG5(YPL227C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:AMD1(YML035C)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:APE3(YBR286W)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APL1(YJR005W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:APN1(YKL114C)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARE2(YNR019W)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ATO3(YDR384C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:ATP3(YBR039W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:COX17(YLL009C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DBP1(YPL119C)|FD-Score:-4.42|P-value:4.88E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DBP7(YKR024C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DCN1(YLR128W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DOA1(YKL213C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DOS2(YDR068W)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERG3(YLR056W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERV25(YML012W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FAR3(YMR052W)|FD-Score:4.92|P-value:4.25E-7||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FMP48(YGR052W_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FPR1(YNL135C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GAC1(YOR178C)|FD-Score:3.92|P-value:4.47E-5||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GLC3(YEL011W)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GLO3(YER122C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GTT3(YEL017W)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HMT1(YBR034C)|FD-Score:4.98|P-value:3.15E-7||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:KAP123(YER110C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KTR2(YKR061W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LST7(YGR057C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MDS3(YGL197W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MEI4(YER044C-A)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MMM1(YLL006W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MPP6(YNR024W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPS28(YDR337W)|FD-Score:5.95|P-value:1.36E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NOT5(YPR072W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OCA1(YNL099C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OCA4(YCR095C_p)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PEP7(YDR323C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PPT2(YPL148C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD18(YCR066W)|FD-Score:6.89|P-value:2.74E-12||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RED1(YLR263W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RPL14A(YKL006W)|FD-Score:7.46|P-value:4.47E-14||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL4A(YBR031W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SDH4(YDR178W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SFA1(YDL168W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHU2(YDR078C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SKG1(YKR100C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SKI2(YLR398C)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SKI3(YPR189W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SRL3(YKR091W)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:TGL3(YMR313C)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:UBP6(YFR010W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UPS3(YDR185C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDL032W(YDL032W_d)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YEL057C(YEL057C_p)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER077C(YER077C_p)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGL034C(YGL034C_d)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR067C(YGR067C_p)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p Gene:YGR146C-A(YGR146C-A_p)|FD-Score:3.89|P-value:4.91E-5||SGD DESC:Putative protein of unknown function Gene:YHR202W(YHR202W_p)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YKL071W(YKL071W_p)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR011C(YKR011C_p)|FD-Score:5.64|P-value:8.26E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR184W(YLR184W_d)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Putative protein of unknown function Gene:YMR057C(YMR057C_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YOL019W(YOL019W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOL098C(YOL098C_p)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Putative metalloprotease Gene:YPR053C(YPR053C_d)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR340W8.633.08E-183.72RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YPL122C4.914.58E-70.31TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YJL054W4.602.14E-60.47TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
YDL108W4.131.83E-50.50KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
YBR190W_d3.621.45E-40.01YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YKL042W3.621.50E-40.13SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YLR355C3.482.50E-40.13ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YLR198C_d3.353.97E-40.06YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YBL105C3.294.97E-40.13PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YBL020W3.167.83E-40.14RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YGL030W3.020.001250.08RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YKL182W2.940.001630.06FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YPL007C2.880.001960.10TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YDL004W2.780.002730.01ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YGL044C2.770.002820.02RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL006W7.464.47E-14RPL14ARibosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
YCR066W6.892.74E-12RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YDR337W5.951.36E-9MRPS28Mitochondrial ribosomal protein of the small subunit
YKR011C_p5.648.26E-9YKR011C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress
YKR024C5.561.33E-8DBP7Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
YKL114C5.511.83E-8APN1Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine
YBR034C4.983.15E-7HMT1Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region.
YMR052W4.924.25E-7FAR3Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YKR091W4.681.44E-6SRL3Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate
YLR184W_d4.621.92E-6YLR184W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR323C4.503.37E-6PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YLR398C4.385.95E-6SKI2Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
YML035C4.356.93E-6AMD1AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
YDR216W4.347.08E-6ADR1Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization
YDR408C4.308.45E-6ADE8Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway

GO enrichment analysis for SGTC_2099
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1433.38E-28SGTC_246cycloheximide 667.0 μMICCB bioactive library167600400.0806452
0.0859.96E-11SGTC_448monastrol 171.0 μMICCB bioactive library29879270.0694444
0.0823.00E-10SGTC_9322857-0803 154.0 μMChemDiv (Drug-like library)6748980.103448
0.0664.18E-7SGTC_1768st044301 37.2 μMTimTec (Natural product derivative library)39313490.125
0.0631.22E-6SGTC_5504123-0385 25.7 μMChemDiv (Drug-like library)57311230.112676TSC3-RPN4
0.0621.74E-6SGTC_21015377231 179.2 μMChembridge (Fragment library)28442130.534884
0.0613.15E-6SGTC_25354',4'-dimethoxydalbergione 51.2 μMMicrosource (Natural product library)3641060.0806452DNA damage response
0.0604.74E-6SGTC_32449134489 49.5 μMChembridge (Drug-like library)165842710.0810811
0.0595.07E-6SGTC_23077538926 6.3 μMChembridge (Fragment library)965820.0980392
0.0596.17E-6SGTC_1536cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid 61.9 μMTimTec (Pure natural product library)665350.0833333
0.0587.43E-6SGTC_7473448-8087 38.7 μMChemDiv (Drug-like library)51687030.140845
0.0571.03E-5SGTC_11791021-1431 9.3 μMChemDiv (Drug-like library)28409580.135593
0.0571.28E-5SGTC_1071trifluoperazine 6.3 μMNIH Clinical Collection29135350.132353
0.0561.94E-5SGTC_60844-0013 19.8 μMChemDiv (Drug-like library)15849000.0625
0.0543.14E-5SGTC_3650121-0052 13.6 μMChemDiv (Drug-like library)31017390.0769231mitochondrial response to ROS

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21005377183190 μM0.6341462844202Chembridge (Fragment library)250.316762.6631460S ribosome export
SGTC_21015377231179.23 μM0.5348842844213Chembridge (Fragment library)256.708662.35514
SGTC_21215257820149.64 μM0.425791256Chembridge (Fragment library)204.225180.85313
SGTC_1882561564320 μM0.3442622253075Miscellaneous392.8979635.84215TRP & mitochondrial translation
SGTC_242856156437.78 μM0.3442622253075Miscellaneous392.8979635.84215
SGTC_20875276107200 μM0.340426144405Chembridge (Fragment library)238.670241.51713
SGTC_21925915882198.49 μM0.3333332876149Chembridge (Fragment library)268.310441.5513RNA processing & uracil transport
SGTC_24015128683200 μM0.333333708373Miscellaneous344.363343.86214
SGTC_2453573113222.18 μM0.3333331362040Miscellaneous436.266794.16614
SGTC_2426558204632.21 μM0.317462251934Miscellaneous386.3968033.64817
SGTC_21045389443200 μM0.31252244536Chembridge (Fragment library)236.1991630.94714