5377231

3-[(2-chloroanilino)methyl]-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2101
Screen concentration 179.2 μM
Source Chembridge (Fragment library)
PubChem CID 2844213
SMILES C1C(=O)N(C(=O)S1)CNC2=CC=CC=C2Cl
Standardized SMILES Clc1ccccc1NCN2C(=O)CSC2=O
Molecular weight 256.7087
ALogP 2.36
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.09
% growth inhibition (Hom. pool) 1.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2844213
Download HIP data (tab-delimited text)  (excel)
Gene:ERG10(YPL028W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.17||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:FAP7(YDL166C)|FD-Score:-3.67|P-value:1.21E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:JIP5(YPR169W)|FD-Score:3.97|P-value:3.58E-5|Clearance:0.11||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:LUC7(YDL087C)|FD-Score:-5.75|P-value:4.52E-9|Clearance:0||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:NOP9(YJL010C)|FD-Score:3.12|P-value:9.01E-4|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NUS1(YDL193W)|FD-Score:3.86|P-value:5.66E-5|Clearance:0.02||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:RPP0(YLR340W)|FD-Score:3.39|P-value:3.47E-4|Clearance:0.05||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPT1(YKL145W)|FD-Score:4.42|P-value:4.95E-6|Clearance:0.45||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:TCP1(YDR212W)|FD-Score:3.74|P-value:9.07E-5|Clearance:0.18||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFB2(YPL122C)|FD-Score:3.13|P-value:8.84E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TFC8(YPL007C)|FD-Score:7.95|P-value:9.34E-16|Clearance:3.28||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:TSC11(YER093C)|FD-Score:3.34|P-value:4.21E-4|Clearance:0.21||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:UGP1(YKL035W)|FD-Score:3.84|P-value:6.16E-5|Clearance:0.1||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YLR198C(YLR198C_d)|FD-Score:4.67|P-value:1.49E-6|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.12|P-value:9.15E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:ERG10(YPL028W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.17||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:FAP7(YDL166C)|FD-Score:-3.67|P-value:1.21E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:JIP5(YPR169W)|FD-Score:3.97|P-value:3.58E-5|Clearance:0.11||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:LUC7(YDL087C)|FD-Score:-5.75|P-value:4.52E-9|Clearance:0||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:NOP9(YJL010C)|FD-Score:3.12|P-value:9.01E-4|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:NUS1(YDL193W)|FD-Score:3.86|P-value:5.66E-5|Clearance:0.02||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:RPP0(YLR340W)|FD-Score:3.39|P-value:3.47E-4|Clearance:0.05||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPT1(YKL145W)|FD-Score:4.42|P-value:4.95E-6|Clearance:0.45||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:TCP1(YDR212W)|FD-Score:3.74|P-value:9.07E-5|Clearance:0.18||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFB2(YPL122C)|FD-Score:3.13|P-value:8.84E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TFC8(YPL007C)|FD-Score:7.95|P-value:9.34E-16|Clearance:3.28||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:TSC11(YER093C)|FD-Score:3.34|P-value:4.21E-4|Clearance:0.21||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:UGP1(YKL035W)|FD-Score:3.84|P-value:6.16E-5|Clearance:0.1||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YLR198C(YLR198C_d)|FD-Score:4.67|P-value:1.49E-6|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.12|P-value:9.15E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2844213
Download HOP data (tab-delimited text)  (excel)
Gene:ADH5(YBR145W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AIM33(YML087C)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:API2(YDR525W_d)|FD-Score:4.31|P-value:8.24E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:BAG7(YOR134W)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BCH1(YMR237W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BRE1(YDL074C)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:COQ3(YOL096C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:CPA2(YJR109C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CTR1(YPR124W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:ECM4(YKR076W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ENV11(YGR071C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:FMP48(YGR052W_p)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GCS1(YDL226C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEP3(YOR205C)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GIR2(YDR152W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GRH1(YDR517W)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress Gene:IST3(YIR005W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:MDJ2(YNL328C)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MET28(YIR017C)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MPD2(YOL088C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:NEJ1(YLR265C)|FD-Score:3.8|P-value:7.31E-5||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PES4(YFR023W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET122(YER153C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PRR2(YDL214C)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PYC2(YBR218C)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RMD6(YEL072W)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Protein required for sporulation Gene:SEC22(YLR268W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SKN1(YGR143W)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SPG3(YDR504C)|FD-Score:-4.27|P-value:9.60E-6||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SRO7(YPR032W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:TMA108(YIL137C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TPM1(YNL079C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:UBX4(YMR067C)|FD-Score:6.51|P-value:3.73E-11||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:URN1(YPR152C)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:WSS1(YHR134W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YGL042C(YGL042C_d)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YGR051C(YGR051C_d)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YLR149C(YLR149C_p)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR456W(YLR456W_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YLR460C(YLR460C_p)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YOR1(YGR281W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPS6(YIR039C)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:ADH5(YBR145W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AIM33(YML087C)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:API2(YDR525W_d)|FD-Score:4.31|P-value:8.24E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:BAG7(YOR134W)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:BCH1(YMR237W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BRE1(YDL074C)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:COQ3(YOL096C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:CPA2(YJR109C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CTR1(YPR124W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:ECM4(YKR076W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ENV11(YGR071C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:FMP48(YGR052W_p)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GCS1(YDL226C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEP3(YOR205C)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GIR2(YDR152W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GRH1(YDR517W)|FD-Score:-3.17|P-value:7.51E-4||SGD DESC:Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress Gene:IST3(YIR005W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:MDJ2(YNL328C)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MET28(YIR017C)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MPD2(YOL088C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:NEJ1(YLR265C)|FD-Score:3.8|P-value:7.31E-5||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PES4(YFR023W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET122(YER153C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PRR2(YDL214C)|FD-Score:3.94|P-value:4.16E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PYC2(YBR218C)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RMD6(YEL072W)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Protein required for sporulation Gene:SEC22(YLR268W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SKN1(YGR143W)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SPG3(YDR504C)|FD-Score:-4.27|P-value:9.60E-6||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SRO7(YPR032W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:TMA108(YIL137C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TPM1(YNL079C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:UBX4(YMR067C)|FD-Score:6.51|P-value:3.73E-11||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:URN1(YPR152C)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:WSS1(YHR134W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YGL042C(YGL042C_d)|FD-Score:-3.16|P-value:7.97E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YGR051C(YGR051C_d)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YLR149C(YLR149C_p)|FD-Score:-3.14|P-value:8.51E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR456W(YLR456W_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YLR460C(YLR460C_p)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YOR1(YGR281W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPS6(YIR039C)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL007C7.959.34E-163.28TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YLR198C_d4.671.49E-60.25YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YKL145W4.424.95E-60.45RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YPR169W3.973.58E-50.11JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YDL193W3.865.66E-50.02NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YKL035W3.846.16E-50.10UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YDR212W3.749.07E-50.18TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YPL028W3.571.81E-40.17ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YLR340W3.393.47E-40.05RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YER093C3.344.21E-40.21TSC11Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
YPL122C3.138.84E-40.01TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YJL010C3.129.01E-40.00NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YMR290W-A_d3.129.15E-40.03YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YJL031C3.080.001020.03BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YKL192C3.050.001130.14ACP1Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR067C6.513.73E-11UBX4UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
YBR218C5.161.26E-7PYC2Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
YOR134W5.032.44E-7BAG7Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p
YPR152C4.631.82E-6URN1Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase
YDR525W_d4.318.24E-6API2_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
YJR109C4.151.65E-5CPA2Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor
YDL214C3.944.16E-5PRR2Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance
YOR205C3.885.18E-5GEP3Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1)
YGR052W_p3.885.21E-5FMP48_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
YLR265C3.807.31E-5NEJ1Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p
YER153C3.691.11E-4PET122Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
YPR032W3.561.83E-4SRO7Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
YDR152W3.522.14E-4GIR2Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein
YIL137C3.413.27E-4TMA108Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease
YIR005W3.344.19E-4IST3Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing

GO enrichment analysis for SGTC_2101
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1232.71E-21SGTC_1689st020265 53.5 μMTimTec (Natural product derivative library)57583200.0694444
0.0912.40E-12SGTC_20855307070 144.0 μMChembridge (Fragment library)28403510.0821918superoxide
0.0621.74E-6SGTC_20995377090 177.4 μMChembridge (Fragment library)22438290.534884
0.0622.08E-6SGTC_22077258803 200.0 μMChembridge (Fragment library)36667990.105263
0.0594.81E-6SGTC_3071501-1223 22.3 μMChemDiv (Drug-like library)28303290.106061
0.0587.02E-6SGTC_20965468907 200.0 μMChembridge (Fragment library)7597850.0615385
0.0571.10E-5SGTC_20845306078 60.6 μMChembridge (Fragment library)6793400.0655738
0.0571.29E-5SGTC_1688st021091 57.6 μMTimTec (Natural product derivative library)36563660.0487805
0.0543.10E-5SGTC_287k060-0039 29.6 μMChemDiv (Drug-like library)58619740.138462
0.0501.08E-4SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride 61.8 μMTimTec (Natural product derivative library)170377580.134328ergosterol biosynthesis
0.0472.97E-4SGTC_1063disulfiram 1.8 μMNIH Clinical Collection31170.0204082
0.0455.10E-4SGTC_260302-0163 425.2 μMChemDiv (Drug-like library)31009150.155172heme biosynthesis & mitochondrial translocase
0.0455.61E-4SGTC_8530419-0306 21.0 μMChemDiv (Drug-like library)7627130.0806452
0.0430.00101SGTC_22095-(5-methyl-2-thenylidene)-Barbituric acid 122.8 μMChembridge (Fragment library)6167640.0877193
0.0430.00105SGTC_32039113543 49.5 μMChembridge (Drug-like library)170180120.116667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20995377090177.37 μM0.5348842243829Chembridge (Fragment library)240.2540631.89615
SGTC_21005377183190 μM0.52844202Chembridge (Fragment library)250.316762.6631460S ribosome export
SGTC_1922557818725 μM0.4716981350412Miscellaneous362.8057834.45715TRP & mitochondrial translation
SGTC_2433560787427.82 μM0.3965522252428Miscellaneous374.84134.23515
SGTC_21215257820149.64 μM0.372093791256Chembridge (Fragment library)204.225180.85313
SGTC_20875276107200 μM0.354167144405Chembridge (Fragment library)238.670241.51713
SGTC_2450573973712.93 μM0.3454552262825Miscellaneous328.3607233.79315
SGTC_2895905444758.44 μM0.3148152697450Chembridge (Drug-like library)343.850483.16113
SGTC_2994905352071.43 μM0.3090914647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_23819035592200 μM0.34749657Chembridge (Fragment library)243.70991.68313