5377816

(5E)-5-(furan-2-ylmethylidene)-1,3-thiazolidine-2,4-dione

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2102
Screen concentration 72.8 μM
Source Chembridge (Fragment library)
PubChem CID 1379702
SMILES C1=COC(=C1)C=C2C(=O)NC(=O)S2
Standardized SMILES O=C1NC(=O)C(=Cc2occc2)S1
Molecular weight 195.1952
ALogP 1.34
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.22
% growth inhibition (Hom. pool) 4.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1379702
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.33||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ATM1(YMR301C)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.02||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:ESP1(YGR098C)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.03||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:KAP95(YLR347C)|FD-Score:-3.1|P-value:9.52E-4|Clearance:0||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:LSG1(YGL099W)|FD-Score:-3.14|P-value:8.59E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LUC7(YDL087C)|FD-Score:-3.8|P-value:7.15E-5|Clearance:0||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MDN1(YLR106C)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.33||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NAR1(YNL240C)|FD-Score:3.21|P-value:6.67E-4|Clearance:0.22||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NUS1(YDL193W)|FD-Score:3.77|P-value:8.32E-5|Clearance:0.33||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PIK1(YNL267W)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.33||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RIM2(YBR192W)|FD-Score:4.45|P-value:4.33E-6|Clearance:0.33||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:ROK1(YGL171W)|FD-Score:-3.35|P-value:4.11E-4|Clearance:0||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RRP36(YOR287C)|FD-Score:4.07|P-value:2.32E-5|Clearance:0.33||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:SPB4(YFL002C)|FD-Score:-3.41|P-value:3.22E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SRP21(YKL122C)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.01||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:TAM41(YGR046W)|FD-Score:-3.1|P-value:9.75E-4|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TOM40(YMR203W)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:WRS1(YOL097C)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.17||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGR265W(YGR265W_d)|FD-Score:-3.31|P-value:4.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:ALG14(YBR070C)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.33||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ATM1(YMR301C)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.02||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:ESP1(YGR098C)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.03||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:KAP95(YLR347C)|FD-Score:-3.1|P-value:9.52E-4|Clearance:0||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:LSG1(YGL099W)|FD-Score:-3.14|P-value:8.59E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LUC7(YDL087C)|FD-Score:-3.8|P-value:7.15E-5|Clearance:0||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MDN1(YLR106C)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.33||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NAR1(YNL240C)|FD-Score:3.21|P-value:6.67E-4|Clearance:0.22||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NUS1(YDL193W)|FD-Score:3.77|P-value:8.32E-5|Clearance:0.33||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PIK1(YNL267W)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.33||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RIM2(YBR192W)|FD-Score:4.45|P-value:4.33E-6|Clearance:0.33||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:ROK1(YGL171W)|FD-Score:-3.35|P-value:4.11E-4|Clearance:0||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RRP36(YOR287C)|FD-Score:4.07|P-value:2.32E-5|Clearance:0.33||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:SPB4(YFL002C)|FD-Score:-3.41|P-value:3.22E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SRP21(YKL122C)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.01||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:TAM41(YGR046W)|FD-Score:-3.1|P-value:9.75E-4|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TOM40(YMR203W)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:WRS1(YOL097C)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.17||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGR265W(YGR265W_d)|FD-Score:-3.31|P-value:4.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1379702
Download HOP data (tab-delimited text)  (excel)
Gene:ALF1(YNL148C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARO2(YGL148W)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BSC2(YDR275W)|FD-Score:4.29|P-value:8.91E-6||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:BUD32(YGR262C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CAP2(YIL034C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHA1(YCL064C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CSH1(YBR161W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DER1(YBR201W)|FD-Score:4.54|P-value:2.78E-6||SGD DESC:Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p Gene:DOT6(YER088C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ERD1(YDR414C)|FD-Score:4.59|P-value:2.17E-6||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG4(YGL012W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP4(YOR016C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCN2(YDR283C)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GLO2(YDR272W)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:HNT2(YDR305C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HPR1(YDR138W)|FD-Score:4.88|P-value:5.42E-7||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSL1(YKL101W)|FD-Score:4.27|P-value:9.98E-6||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:IES2(YNL215W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRC20(YLR247C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:KTI12(YKL110C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MEF1(YLR069C)|FD-Score:3.09|P-value:0.00100||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRH4(YGL064C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP8(YKL142W)|FD-Score:4.83|P-value:6.78E-7||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:NIS1(YNL078W)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:PBS2(YJL128C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PBY1(YBR094W)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress Gene:PDX3(YBR035C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PKR1(YMR123W)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPA2(YMR267W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PPE1(YHR075C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PSH1(YOL054W)|FD-Score:3.82|P-value:6.71E-5||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PSY3(YLR376C)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RAM1(YDL090C)|FD-Score:-4.32|P-value:7.71E-6||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RBD2(YPL246C)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:RPL13B(YMR142C)|FD-Score:5.35|P-value:4.48E-8||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGE1(YPR198W)|FD-Score:-3.76|P-value:8.58E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKY1(YMR216C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPR3(YGR059W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SPT10(YJL127C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRB8(YCR081W)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSZ1(YHR064C)|FD-Score:-3.82|P-value:6.64E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:STV1(YMR054W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TMA16(YOR252W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRP1(YDR007W)|FD-Score:4.92|P-value:4.40E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.42|P-value:6.88E-11||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.96|P-value:1.30E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.27|P-value:9.61E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS36(YLR417W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-4.17|P-value:1.52E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR300C(YBR300C_d)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDL068W(YDL068W_d)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:5.01|P-value:2.66E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR018C(YFR018C_p)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Putative protein of unknown function Gene:YIL141W(YIL141W_d)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL053C(YLL053C_p)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR413W(YLR413W_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YML090W(YML090W_d)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR087W(YMR087W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YOL050C(YOL050C_d)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR289W(YOR289W_p)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL272C(YPL272C_p)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:YSP3(YOR003W)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ZRT2(YLR130C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor Gene:ALF1(YNL148C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARO2(YGL148W)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BSC2(YDR275W)|FD-Score:4.29|P-value:8.91E-6||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:BUD32(YGR262C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CAP2(YIL034C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHA1(YCL064C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CSH1(YBR161W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DER1(YBR201W)|FD-Score:4.54|P-value:2.78E-6||SGD DESC:Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p Gene:DOT6(YER088C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ERD1(YDR414C)|FD-Score:4.59|P-value:2.17E-6||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG4(YGL012W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP4(YOR016C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCN2(YDR283C)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GLO2(YDR272W)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:HNT2(YDR305C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HPR1(YDR138W)|FD-Score:4.88|P-value:5.42E-7||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSL1(YKL101W)|FD-Score:4.27|P-value:9.98E-6||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:IES2(YNL215W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRC20(YLR247C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:KTI12(YKL110C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MEF1(YLR069C)|FD-Score:3.09|P-value:0.00100||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRH4(YGL064C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP8(YKL142W)|FD-Score:4.83|P-value:6.78E-7||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:NIS1(YNL078W)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:PBS2(YJL128C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PBY1(YBR094W)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress Gene:PDX3(YBR035C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PKR1(YMR123W)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPA2(YMR267W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PPE1(YHR075C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PSH1(YOL054W)|FD-Score:3.82|P-value:6.71E-5||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PSY3(YLR376C)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RAM1(YDL090C)|FD-Score:-4.32|P-value:7.71E-6||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RBD2(YPL246C)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:RPL13B(YMR142C)|FD-Score:5.35|P-value:4.48E-8||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGE1(YPR198W)|FD-Score:-3.76|P-value:8.58E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKY1(YMR216C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPR3(YGR059W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SPT10(YJL127C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SRB8(YCR081W)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSZ1(YHR064C)|FD-Score:-3.82|P-value:6.64E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:STV1(YMR054W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TMA16(YOR252W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRP1(YDR007W)|FD-Score:4.92|P-value:4.40E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.42|P-value:6.88E-11||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.96|P-value:1.30E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.27|P-value:9.61E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS36(YLR417W)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-4.17|P-value:1.52E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR300C(YBR300C_d)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDL068W(YDL068W_d)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:5.01|P-value:2.66E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR018C(YFR018C_p)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Putative protein of unknown function Gene:YIL141W(YIL141W_d)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL053C(YLL053C_p)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR413W(YLR413W_p)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YML090W(YML090W_d)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR087W(YMR087W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YOL050C(YOL050C_d)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR289W(YOR289W_p)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL272C(YPL272C_p)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:YSP3(YOR003W)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ZRT2(YLR130C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR192W4.454.33E-60.34RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YNL267W4.241.14E-50.34PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YBR070C4.181.47E-50.34ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YLR106C4.151.68E-50.34MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
YOR287C4.072.32E-50.34RRP36Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
YDL193W3.778.32E-50.34NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YKL122C3.433.01E-40.01SRP21Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
YMR301C3.423.11E-40.02ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YGR098C3.413.29E-40.03ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YOL097C3.373.70E-40.17WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YNL240C3.216.67E-40.22NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YOR282W_d2.990.001410.05YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YPR175W2.940.001660.14DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YML010W2.800.002590.05SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YLR305C2.750.003010.01STT4Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER090W6.426.88E-11TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YPL246C5.961.23E-9RBD2Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
YKL211C5.961.30E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YMR142C5.354.48E-8RPL13BRibosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
YBR094W5.151.30E-7PBY1Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress
YDR008C_d5.012.66E-7YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR007W4.924.40E-7TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR138W4.885.42E-7HPR1Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
YKL142W4.836.78E-7MRP8Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis
YDR414C4.592.17E-6ERD1Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
YCR081W4.552.74E-6SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YBR201W4.542.78E-6DER1Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p
YFR018C_p4.513.27E-6YFR018C_pPutative protein of unknown function
YOR003W4.503.34E-6YSP3Putative precursor to the subtilisin-like protease III
YDR283C4.434.66E-6GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control

GO enrichment analysis for SGTC_2102
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2441.70E-80SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0196078
0.2391.80E-77SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.183333
0.2343.03E-74SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0526316
0.2324.71E-73SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0862069
0.2194.65E-65SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0461538
0.2181.01E-64SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0625
0.2171.09E-63SGTC_10410206-0046 107.0 μMChemDiv (Drug-like library)504940.118644
0.2161.37E-63SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.380952TRP & mitochondrial translation
0.2151.73E-62SGTC_18825615643 20.0 μMMiscellaneous22530750.142857TRP & mitochondrial translation
0.2101.16E-59SGTC_18815652484 16.0 μMMiscellaneous22551290.161765TRP & mitochondrial translation
0.2091.39E-59SGTC_24885268135 45.7 μMMiscellaneous12703560.291667
0.2081.61E-58SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.0555556
0.2074.00E-58SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.0882353plasma membrane duress
0.2052.35E-57SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0819672
0.2052.60E-57SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0833333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_440929-005561.21 μM0.51379590ChemDiv (Drug-like library)239.678142.60613
SGTC_2429590284464.44 μM0.4883721927790Miscellaneous225.287382.44204
SGTC_8920929-007629 μM0.4871795751038ChemDiv (Drug-like library)219.259662.42813TRP & mitochondrial translation
SGTC_12840929-004316 μM0.4871791239883ChemDiv (Drug-like library)223.2235432.14714
SGTC_205252651827.14 μM0.4871791239884Chembridge (Fragment library)223.2235432.14714
SGTC_380929-005158.27 μM0.476191379719ChemDiv (Drug-like library)223.2235432.14714
SGTC_245553777129.75 μM0.476192243908Miscellaneous219.259662.42813
SGTC_2165572719732.14 μM0.4634151361712Chembridge (Fragment library)211.26081.60414
SGTC_2452586313648.41 μM0.4565222184096Miscellaneous253.340543.16804
SGTC_24625162487171.77 μM0.4565222259986Miscellaneous239.313962.79104