5389443

1-[(4-fluorophenyl)methyl]-1,3-diazinane-2,4,6-trione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2104
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 2244536
SMILES C1C(=O)NC(=O)N(C1=O)CC2=CC=C(C=C2)F
Standardized SMILES Fc1ccc(CN2C(=O)CC(=O)NC2=O)cc1
Molecular weight 236.1992
ALogP 0.95
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.6
% growth inhibition (Hom. pool) 1.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2244536
Download HIP data (tab-delimited text)  (excel)
Gene:CAB1(YDR531W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.16||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:DPM1(YPR183W)|FD-Score:3.12|P-value:9.06E-4|Clearance:0.13||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:FMN1(YDR236C)|FD-Score:4.08|P-value:2.26E-5|Clearance:0.03||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GCD1(YOR260W)|FD-Score:3.82|P-value:6.65E-5|Clearance:0.17||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:LSM5(YER146W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.13||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NAF1(YNL124W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.04||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NOG1(YPL093W)|FD-Score:4.05|P-value:2.53E-5|Clearance:0.06||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NUP1(YOR098C)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.21||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:PET9(YBL030C)|FD-Score:3.83|P-value:6.43E-5|Clearance:0.01||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:PRI2(YKL045W)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.16||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPP1(YHR062C)|FD-Score:4.32|P-value:7.93E-6|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TAF7(YMR227C)|FD-Score:4.77|P-value:9.02E-7|Clearance:0.46||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:YKL036C(YKL036C_d)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:CAB1(YDR531W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.16||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:DPM1(YPR183W)|FD-Score:3.12|P-value:9.06E-4|Clearance:0.13||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:FMN1(YDR236C)|FD-Score:4.08|P-value:2.26E-5|Clearance:0.03||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GCD1(YOR260W)|FD-Score:3.82|P-value:6.65E-5|Clearance:0.17||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:LSM5(YER146W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.13||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NAF1(YNL124W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.04||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NOG1(YPL093W)|FD-Score:4.05|P-value:2.53E-5|Clearance:0.06||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NUP1(YOR098C)|FD-Score:3.33|P-value:4.36E-4|Clearance:0.21||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:PET9(YBL030C)|FD-Score:3.83|P-value:6.43E-5|Clearance:0.01||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:PRI2(YKL045W)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.16||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPP1(YHR062C)|FD-Score:4.32|P-value:7.93E-6|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TAF7(YMR227C)|FD-Score:4.77|P-value:9.02E-7|Clearance:0.46||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:YKL036C(YKL036C_d)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2244536
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM3(YBR108W)|FD-Score:3.84|P-value:6.03E-5||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALK1(YGL021W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:AQY1(YPR192W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ARR3(YPR201W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATG7(YHR171W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP17(YDR377W)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BIO4(YNR057C)|FD-Score:4.54|P-value:2.78E-6||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:CAN1(YEL063C)|FD-Score:-4.77|P-value:9.23E-7||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CCE1(YKL011C)|FD-Score:4.28|P-value:9.15E-6||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CHS3(YBR023C)|FD-Score:-3.17|P-value:7.64E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COQ4(YDR204W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:CTF18(YMR078C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTR1(YPR124W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DPB3(YBR278W)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ELM1(YKL048C)|FD-Score:4.37|P-value:6.10E-6||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FMP23(YBR047W_p)|FD-Score:4.94|P-value:3.99E-7||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIM4(YEL003W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:IKI3(YLR384C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:INM1(YHR046C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:ISA2(YPR067W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:JHD1(YER051W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:LNP1(YHR192W_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MAC1(YMR021C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MKK2(YPL140C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MLC2(YPR188C)|FD-Score:-5.03|P-value:2.49E-7||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MNT3(YIL014W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MZM1(YDR493W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NBA1(YOL070C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NGL2(YMR285C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NHX1(YDR456W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:PDR8(YLR266C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO87(YCR037C)|FD-Score:6.9|P-value:2.58E-12||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:REX3(YLR107W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases Gene:RIF2(YLR453C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL14A(YKL006W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPS7B(YNL096C)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRT6(YGL146C_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:SDP1(YIL113W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SRF1(YDL133W)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:TDA7(YNL176C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TMA20(YER007C-A)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TOR1(YJR066W)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URA1(YKL216W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:URA7(YBL039C)|FD-Score:-3.82|P-value:6.77E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCR061W(YCR061W_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YCR102C(YCR102C_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YEL067C(YEL067C_p)|FD-Score:7.02|P-value:1.10E-12||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YER084W(YER084W_p)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YGL041C(YGL041C_d)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL149W(YGL149W_d)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR290W(YGR290W_d)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YHL042W(YHL042W_p)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YIR018C-A(YIR018C-A_p)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YMR253C(YMR253C_p)|FD-Score:7.3|P-value:1.41E-13||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:-5.48|P-value:2.18E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOL029C(YOL029C_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YPR089W(YPR089W_p)|FD-Score:-3.73|P-value:9.43E-5||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPS1(YLR120C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPS3(YLR121C)|FD-Score:-3.77|P-value:8.32E-5||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YSP2(YDR326C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ADE1(YAR015W)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM3(YBR108W)|FD-Score:3.84|P-value:6.03E-5||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALK1(YGL021W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:AQY1(YPR192W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ARR3(YPR201W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATG7(YHR171W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP17(YDR377W)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BIO4(YNR057C)|FD-Score:4.54|P-value:2.78E-6||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:CAN1(YEL063C)|FD-Score:-4.77|P-value:9.23E-7||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CCE1(YKL011C)|FD-Score:4.28|P-value:9.15E-6||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CHS3(YBR023C)|FD-Score:-3.17|P-value:7.64E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:COQ4(YDR204W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:CTF18(YMR078C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTR1(YPR124W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DPB3(YBR278W)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ELM1(YKL048C)|FD-Score:4.37|P-value:6.10E-6||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FMP23(YBR047W_p)|FD-Score:4.94|P-value:3.99E-7||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIM4(YEL003W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:IKI3(YLR384C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:INM1(YHR046C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:ISA2(YPR067W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:JHD1(YER051W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:LNP1(YHR192W_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MAC1(YMR021C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MKK2(YPL140C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MLC2(YPR188C)|FD-Score:-5.03|P-value:2.49E-7||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MNT3(YIL014W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MZM1(YDR493W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NBA1(YOL070C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NGL2(YMR285C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NHX1(YDR456W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:PDR8(YLR266C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO87(YCR037C)|FD-Score:6.9|P-value:2.58E-12||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:REX3(YLR107W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases Gene:RIF2(YLR453C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL14A(YKL006W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPS7B(YNL096C)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRT6(YGL146C_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:SDP1(YIL113W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SRF1(YDL133W)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:TDA7(YNL176C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TMA20(YER007C-A)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TOR1(YJR066W)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URA1(YKL216W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:URA7(YBL039C)|FD-Score:-3.82|P-value:6.77E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCR061W(YCR061W_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YCR102C(YCR102C_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YEL067C(YEL067C_p)|FD-Score:7.02|P-value:1.10E-12||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YER084W(YER084W_p)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YGL041C(YGL041C_d)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL149W(YGL149W_d)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR290W(YGR290W_d)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YHL042W(YHL042W_p)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YIR018C-A(YIR018C-A_p)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YMR253C(YMR253C_p)|FD-Score:7.3|P-value:1.41E-13||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:-5.48|P-value:2.18E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOL029C(YOL029C_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YPR089W(YPR089W_p)|FD-Score:-3.73|P-value:9.43E-5||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPS1(YLR120C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPS3(YLR121C)|FD-Score:-3.77|P-value:8.32E-5||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YSP2(YDR326C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR227C4.779.02E-70.46TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YHR062C4.327.93E-60.12RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL036C_d4.191.37E-50.11YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YDR236C4.082.26E-50.03FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YPL093W4.052.53E-50.06NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YDR531W3.993.28E-50.16CAB1Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
YBL030C3.836.43E-50.01PET9Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
YOR260W3.826.65E-50.17GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YER146W3.651.31E-40.13LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YKL045W3.522.12E-40.16PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YNL124W3.363.84E-40.04NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YOR098C3.334.36E-40.21NUP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
YPR183W3.129.06E-40.13DPM1Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation
YBR254C2.990.001410.05TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
YNL075W2.930.001680.01IMP4Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR253C_p7.301.41E-13YMR253C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
YEL067C_p7.021.10E-12YEL067C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YCR037C6.902.58E-12PHO87Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
YBR047W_p4.943.99E-7FMP23_pPutative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YNR057C4.542.78E-6BIO4Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels
YKL048C4.376.10E-6ELM1Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring
YKL011C4.289.15E-6CCE1Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA
YKL216W4.241.13E-5URA1Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
YMR078C4.211.26E-5CTF18Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
YPR124W4.191.37E-5CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YMR021C4.102.03E-5MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YGL041C_d4.102.11E-5YGL041C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR278W4.013.01E-5DPB3Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
YOL070C3.973.65E-5NBA1Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate
YGL021W3.953.93E-5ALK1Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins

GO enrichment analysis for SGTC_2104
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1181.20E-19SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.163636TSC3-RPN4
0.1172.31E-19SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0508475TSC3-RPN4
0.1055.77E-16SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.0757576TSC3-RPN4
0.1016.11E-15SGTC_21135491891 107.0 μMChembridge (Fragment library)7899790.0909091TSC3-RPN4
0.0952.73E-13SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.149254
0.0953.31E-13SGTC_2742clothiapine 36.3 μMMiscellaneous163510.0746269NEO1
0.0944.28E-13SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0972222TSC3-RPN4
0.0921.32E-12SGTC_22226885208 172.1 μMChembridge (Fragment library)6095140.148148
0.0921.36E-12SGTC_1612st002233 45.4 μMTimTec (Natural product derivative library)10350210.0649351
0.0905.00E-12SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.118421TSC3-RPN4
0.0899.00E-12SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.05TSC3-RPN4
0.0881.34E-11SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0789474TSC3-RPN4
0.0872.75E-11SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0909091TSC3-RPN4
0.0862.88E-11SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.111111TSC3-RPN4
0.0864.11E-11SGTC_21515563267 85.4 μMChembridge (Fragment library)53108240.0517241

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_169k015-001716.03 μM0.3751840002ChemDiv (Drug-like library)334.1398633.41303
SGTC_21846075246200 μM0.3478262267320Chembridge (Fragment library)260.288442.13513
SGTC_1434032-119415.74 μM0.3461541806963ChemDiv (Drug-like library)329.3454833.69515
SGTC_23939031399185.94 μM0.3404261526623Chembridge (Fragment library)218.212-0.59324RNA processing & uracil transport
SGTC_11594092-053861.2 μM0.313726717301ChemDiv (Drug-like library)332.7997233.65403
SGTC_23427990637118.86 μM0.3137262033102Chembridge (Fragment library)242.23341.07123RNA processing & uracil transport
SGTC_20995377090177.37 μM0.31252243829Chembridge (Fragment library)240.2540631.89615
SGTC_2237666774747.22 μM0.306122729940Chembridge (Fragment library)236.2653632.24314
SGTC_10194262-02123.4 μM0.2830194677213ChemDiv (Drug-like library)352.4881635.20803ergosterol depletion effects on membrane
SGTC_2880904298358.44 μM0.2758626464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)