5488546

2,6-diamino-4-cyclohexyl-4H-thiopyran-3,5-dicarbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2112
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 600757
SMILES C1CCC(CC1)C2C(=C(SC(=C2C#N)N)N)C#N
Standardized SMILES NC1=C(C#N)C(C2CCCCC2)C(=C(N)S1)C#N
Molecular weight 260.3579
ALogP 2.14
H-bond donor count 2
H-bond acceptor count 5
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.28
% growth inhibition (Hom. pool) 5.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 600757
Download HIP data (tab-delimited text)  (excel)
Gene:CCT4(YDL143W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.03||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.08||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:FCF2(YLR051C)|FD-Score:5.89|P-value:1.91E-9|Clearance:1.16||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GCD11(YER025W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.03||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:LAS1(YKR063C)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MIF2(YKL089W)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.06||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:PBN1(YCL052C)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.13||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PGA1(YNL158W)|FD-Score:3.55|P-value:1.95E-4|Clearance:0||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RET1(YOR207C)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.05||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RIM2(YBR192W)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.01||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPL17A(YKL180W)|FD-Score:4.32|P-value:7.95E-6|Clearance:0.25||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPN2(YIL075C)|FD-Score:4.07|P-value:2.40E-5|Clearance:0.05||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:4.02|P-value:2.96E-5|Clearance:0.35||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPP1(YHR062C)|FD-Score:7.68|P-value:7.83E-15|Clearance:1.79||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP46(YGR095C)|FD-Score:4.73|P-value:1.15E-6|Clearance:0.41||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RSE1(YML049C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.15||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SDS22(YKL193C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.1||SGD DESC:Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function Gene:TAD2(YJL035C)|FD-Score:3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:YAE1(YJR067C)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.09||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:CCT4(YDL143W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.03||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.08||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:FCF2(YLR051C)|FD-Score:5.89|P-value:1.91E-9|Clearance:1.16||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GCD11(YER025W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.03||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:LAS1(YKR063C)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MIF2(YKL089W)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.06||SGD DESC:Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 Gene:PBN1(YCL052C)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.13||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PGA1(YNL158W)|FD-Score:3.55|P-value:1.95E-4|Clearance:0||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RET1(YOR207C)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.05||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RIM2(YBR192W)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.01||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPL17A(YKL180W)|FD-Score:4.32|P-value:7.95E-6|Clearance:0.25||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPN2(YIL075C)|FD-Score:4.07|P-value:2.40E-5|Clearance:0.05||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:4.02|P-value:2.96E-5|Clearance:0.35||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPP1(YHR062C)|FD-Score:7.68|P-value:7.83E-15|Clearance:1.79||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP46(YGR095C)|FD-Score:4.73|P-value:1.15E-6|Clearance:0.41||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RSE1(YML049C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.15||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SDS22(YKL193C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.1||SGD DESC:Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function Gene:TAD2(YJL035C)|FD-Score:3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:YAE1(YJR067C)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.09||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 600757
Download HOP data (tab-delimited text)  (excel)
Gene:ABF2(YMR072W)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ACM1(YPL267W)|FD-Score:4.81|P-value:7.68E-7||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ARO80(YDR421W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:ASN2(YGR124W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:BDH1(YAL060W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BUD27(YFL023W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:BZZ1(YHR114W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CAN1(YEL063C)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:COX14(YML129C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CSR1(YLR380W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CST9(YLR394W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DOT1(YDR440W)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DPB3(YBR278W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:EFG1(YGR271C-A)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELM1(YKL048C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG3(YLR056W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FUN30(YAL019W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GCV1(YDR019C)|FD-Score:4.85|P-value:6.19E-7||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GGC1(YDL198C)|FD-Score:3.82|P-value:6.71E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GUP1(YGL084C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GZF3(YJL110C)|FD-Score:-4.31|P-value:8.00E-6||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HEF3(YNL014W)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HHT1(YBR010W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HIS5(YIL116W)|FD-Score:-4.39|P-value:5.56E-6||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IES4(YOR189W)|FD-Score:5|P-value:2.93E-7||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IME4(YGL192W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRC18(YJL037W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISU2(YOR226C)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:JEM1(YJL073W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:JHD2(YJR119C)|FD-Score:4.43|P-value:4.61E-6||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:KTR3(YBR205W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress Gene:LCL3(YGL085W_p)|FD-Score:5.52|P-value:1.70E-8||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LEM3(YNL323W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSC2(YGR244C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAG2(YLR427W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MAM1(YER106W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MBP1(YDL056W)|FD-Score:6.2|P-value:2.87E-10||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MPD1(YOR288C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRC1(YCL061C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL11(YDL202W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAP1(YKR048C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:OPI9(YLR338W_d)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PBP1(YGR178C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PDH1(YPR002W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDX1(YGR193C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PET127(YOR017W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PHM6(YDR281C)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PRS3(YHL011C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:REG1(YDR028C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REV7(YIL139C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RFM1(YOR279C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPA12(YJR063W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPN4(YDL020C)|FD-Score:4.93|P-value:4.18E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RTT102(YGR275W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SCO1(YBR037C)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SDP1(YIL113W)|FD-Score:-5.91|P-value:1.76E-9||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SPT23(YKL020C)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SRB2(YHR041C)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SWF1(YDR126W)|FD-Score:15.1|P-value:7.66E-52||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TSC3(YBR058C-A)|FD-Score:30.9|P-value:8.35E-210||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA4(YHR111W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UTH1(YKR042W)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS69(YPR087W_d)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YAL045C(YAL045C_d)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Putative protein of unknown function Gene:YBR051W(YBR051W_d)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR197C(YBR197C_p)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCR007C(YCR007C_p)|FD-Score:-4.04|P-value:2.63E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR102W-A(YCR102W-A_d)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER084W(YER084W_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFR056C(YFR056C_d)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGL072C(YGL072C_d)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YHR049C-A(YHR049C-A_d)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL055C(YIL055C_p)|FD-Score:-3.81|P-value:7.08E-5||SGD DESC:Putative protein of unknown function Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YLR125W(YLR125W_p)|FD-Score:-3.79|P-value:7.46E-5||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YMR245W(YMR245W_d)|FD-Score:5.47|P-value:2.28E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR073C(YNR073C_p)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL085C(YOL085C_d)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR364W(YOR364W_d)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPR012W(YPR012W_d)|FD-Score:5.09|P-value:1.74E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:ABF2(YMR072W)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ACM1(YPL267W)|FD-Score:4.81|P-value:7.68E-7||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ARO80(YDR421W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:ASN2(YGR124W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:BDH1(YAL060W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:BUD27(YFL023W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:BZZ1(YHR114W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins Gene:CAN1(YEL063C)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:COX14(YML129C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CSR1(YLR380W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CST9(YLR394W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DOT1(YDR440W)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DPB3(YBR278W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:EFG1(YGR271C-A)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELM1(YKL048C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG3(YLR056W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FUN30(YAL019W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GCV1(YDR019C)|FD-Score:4.85|P-value:6.19E-7||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GGC1(YDL198C)|FD-Score:3.82|P-value:6.71E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GUP1(YGL084C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GZF3(YJL110C)|FD-Score:-4.31|P-value:8.00E-6||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HEF3(YNL014W)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HHT1(YBR010W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HIS5(YIL116W)|FD-Score:-4.39|P-value:5.56E-6||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IES4(YOR189W)|FD-Score:5|P-value:2.93E-7||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IME4(YGL192W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRC18(YJL037W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISU2(YOR226C)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:JEM1(YJL073W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:JHD2(YJR119C)|FD-Score:4.43|P-value:4.61E-6||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:KTR3(YBR205W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress Gene:LCL3(YGL085W_p)|FD-Score:5.52|P-value:1.70E-8||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LEM3(YNL323W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSC2(YGR244C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAG2(YLR427W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MAM1(YER106W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MBP1(YDL056W)|FD-Score:6.2|P-value:2.87E-10||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MPD1(YOR288C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRC1(YCL061C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL11(YDL202W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAP1(YKR048C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:OPI9(YLR338W_d)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PBP1(YGR178C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PDH1(YPR002W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDX1(YGR193C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PET127(YOR017W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PHM6(YDR281C)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PRS3(YHL011C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:REG1(YDR028C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REV7(YIL139C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RFM1(YOR279C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPA12(YJR063W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPN4(YDL020C)|FD-Score:4.93|P-value:4.18E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RTT102(YGR275W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SCO1(YBR037C)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SDP1(YIL113W)|FD-Score:-5.91|P-value:1.76E-9||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SPT23(YKL020C)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SRB2(YHR041C)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SWF1(YDR126W)|FD-Score:15.1|P-value:7.66E-52||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TSC3(YBR058C-A)|FD-Score:30.9|P-value:8.35E-210||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA4(YHR111W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UTH1(YKR042W)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS69(YPR087W_d)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YAL045C(YAL045C_d)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Putative protein of unknown function Gene:YBR051W(YBR051W_d)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR197C(YBR197C_p)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCR007C(YCR007C_p)|FD-Score:-4.04|P-value:2.63E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR102W-A(YCR102W-A_d)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER084W(YER084W_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFR056C(YFR056C_d)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGL072C(YGL072C_d)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YHR049C-A(YHR049C-A_d)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL055C(YIL055C_p)|FD-Score:-3.81|P-value:7.08E-5||SGD DESC:Putative protein of unknown function Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YLR125W(YLR125W_p)|FD-Score:-3.79|P-value:7.46E-5||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YMR245W(YMR245W_d)|FD-Score:5.47|P-value:2.28E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR073C(YNR073C_p)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL085C(YOL085C_d)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR364W(YOR364W_d)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPR012W(YPR012W_d)|FD-Score:5.09|P-value:1.74E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C7.687.83E-151.79RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR051C5.891.91E-91.16FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YBR192W4.731.10E-60.01RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YGR095C4.731.15E-60.41RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YKL180W4.327.95E-60.25RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YIL075C4.072.40E-50.05RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YER021W4.022.96E-50.35RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YJR067C3.671.24E-40.09YAE1Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YDL143W3.581.73E-40.03CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YNL158W3.551.95E-46.56E-4PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes
YKL089W3.551.95E-40.06MIF2Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3
YER025W3.482.48E-40.03GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YKL193C3.462.72E-40.10SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YHR107C3.353.97E-40.08CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YCL052C3.285.20E-40.12PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A30.908.35E-210TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR126W15.107.66E-52SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YDL056W6.202.87E-10MBP1Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
YPR002W5.601.07E-8PDH1Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
YGL085W_p5.521.70E-8LCL3_pPutative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS
YMR245W_d5.472.28E-8YMR245W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPR012W_d5.091.74E-7YPR012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene
YOR189W5.002.93E-7IES4Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses
YDL020C4.934.18E-7RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YDR019C4.856.19E-7GCV1T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
YPL267W4.817.68E-7ACM1Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein
YOR226C4.631.81E-6ISU2Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved
YKR042W4.552.69E-6UTH1Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
YJR119C4.434.61E-6JHD2JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation
YCL061C4.161.60E-5MRC1S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres

GO enrichment analysis for SGTC_2112
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5570SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.583333TSC3-RPN4
0.5120SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0422535TSC3-RPN4
0.5080SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0172414TSC3-RPN4
0.4650SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0151515TSC3-RPN4
0.4514.93E-294SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0163934TSC3-RPN4
0.4451.08E-284SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0172414TSC3-RPN4
0.4435.11E-282SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.015625TSC3-RPN4
0.4277.97E-261SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0131579TSC3-RPN4
0.4192.96E-249SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0434783
0.4178.16E-248SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.149254TSC3-RPN4
0.4171.61E-247SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0576923TSC3-RPN4
0.4075.95E-235SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0169492TSC3-RPN4
0.3974.31E-222SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0178571TSC3-RPN4
0.3969.92E-221SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0483871TSC3-RPN4
0.3912.48E-215SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.348837TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21105483698200 μM0.5833332848765Chembridge (Fragment library)258.342061.69125TSC3-RPN4
SGTC_20725231554129 μM0.483871789545Chembridge (Fragment library)192.240920.43125
SGTC_20795212404121 μM0.416667758462Chembridge (Fragment library)220.294081.34325
SGTC_20865274770176.62 μM0.405405689278Chembridge (Fragment library)254.31031.46325
SGTC_20785212016200 μM0.3846151899708Chembridge (Fragment library)288.755362.1272560S ribosome export
SGTC_21135491891107.03 μM0.375789979Chembridge (Fragment library)268.336881.91925TSC3-RPN4
SGTC_2126525117433.88 μM0.375790722Chembridge (Fragment library)178.214340.17925
SGTC_2071523095139.92 μM0.3658541799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4
SGTC_2074521200775.4 μM0.365854758611Chembridge (Fragment library)272.3007631.66826
SGTC_20775212015200 μM0.365854780900Chembridge (Fragment library)288.755362.12725