5491891

2,6-diamino-4-benzyl-4H-thiopyran-3,5-dicarbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2113
Screen concentration 107.0 μM
Source Chembridge (Fragment library)
PubChem CID 789979
SMILES C1=CC=C(C=C1)CC2C(=C(SC(=C2C#N)N)N)C#N
Standardized SMILES NC1=C(C#N)C(Cc2ccccc2)C(=C(N)S1)C#N
Molecular weight 268.3369
ALogP 1.92
H-bond donor count 2
H-bond acceptor count 5
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.47
% growth inhibition (Hom. pool) 4.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 789979
Download HIP data (tab-delimited text)  (excel)
Gene:ARP3(YJR065C)|FD-Score:3.12|P-value:9.18E-4|Clearance:0.03||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CAB3(YKL088W)|FD-Score:3.79|P-value:7.64E-5|Clearance:0.26||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CBK1(YNL161W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.04||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:LAS1(YKR063C)|FD-Score:3.4|P-value:3.41E-4|Clearance:0.2||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MEC1(YBR136W)|FD-Score:6.39|P-value:8.28E-11|Clearance:0.31||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:RER2(YBR002C)|FD-Score:4.29|P-value:8.79E-6|Clearance:0.17||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET1(YOR207C)|FD-Score:6.08|P-value:6.13E-10|Clearance:1.51||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC53(YDL150W)|FD-Score:4.56|P-value:2.52E-6|Clearance:0.27||SGD DESC:RNA polymerase III subunit C53 Gene:RPL3(YOR063W)|FD-Score:3.89|P-value:5.11E-5|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RPP1(YHR062C)|FD-Score:8.54|P-value:6.87E-18|Clearance:2.15||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP46(YGR095C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.24||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:YDL152W(YDL152W_d)|FD-Score:3.19|P-value:6.99E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YPR142C(YPR142C_d)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:ARP3(YJR065C)|FD-Score:3.12|P-value:9.18E-4|Clearance:0.03||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CAB3(YKL088W)|FD-Score:3.79|P-value:7.64E-5|Clearance:0.26||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:CBK1(YNL161W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.04||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:LAS1(YKR063C)|FD-Score:3.4|P-value:3.41E-4|Clearance:0.2||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MEC1(YBR136W)|FD-Score:6.39|P-value:8.28E-11|Clearance:0.31||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:RER2(YBR002C)|FD-Score:4.29|P-value:8.79E-6|Clearance:0.17||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET1(YOR207C)|FD-Score:6.08|P-value:6.13E-10|Clearance:1.51||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC53(YDL150W)|FD-Score:4.56|P-value:2.52E-6|Clearance:0.27||SGD DESC:RNA polymerase III subunit C53 Gene:RPL3(YOR063W)|FD-Score:3.89|P-value:5.11E-5|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:RPP1(YHR062C)|FD-Score:8.54|P-value:6.87E-18|Clearance:2.15||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP46(YGR095C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.24||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:YDL152W(YDL152W_d)|FD-Score:3.19|P-value:6.99E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YPR142C(YPR142C_d)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 789979
Download HOP data (tab-delimited text)  (excel)
Gene:ABF2(YMR072W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ABP140(YOR239W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ALD5(YER073W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:ALF1(YNL148C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APT2(YDR441C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ATG7(YHR171W)|FD-Score:6.56|P-value:2.68E-11||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP14(YLR295C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP15(YPL271W)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ATP5(YDR298C)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:AUS1(YOR011W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BDS1(YOL164W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:CAR2(YLR438W)|FD-Score:-3.11|P-value:9.30E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CCS1(YMR038C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN5(YOR028C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CUE5(YOR042W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUP9(YPL177C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DCW1(YKL046C)|FD-Score:-3.89|P-value:5.09E-5||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DIA4(YHR011W)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DOG2(YHR043C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:ECM32(YER176W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ECM8(YBR076W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Non-essential protein of unknown function Gene:FIG4(YNL325C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FMP23(YBR047W_p)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV6(YNL133C)|FD-Score:-5.3|P-value:5.89E-8||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GGC1(YDL198C)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GLC8(YMR311C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HCS1(YKL017C)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HHF1(YBR009C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HST2(YPL015C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IRE1(YHR079C)|FD-Score:-3.96|P-value:3.79E-5||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LDB18(YLL049W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:MDM1(YML104C)|FD-Score:-5.87|P-value:2.18E-9||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MET12(YPL023C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MRPL49(YJL096W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS17(YMR188C)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC6(YOR354C)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MSF1(YPR047W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MST1(YKL194C)|FD-Score:4.29|P-value:8.83E-6||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC6(YHR151C)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NDE1(YMR145C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NSI1(YDR026C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication Gene:OAR1(YKL055C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OSW1(YOR255W)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PBP1(YGR178C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PEP7(YDR323C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PIF1(YML061C)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RER1(YCL001W)|FD-Score:-5.4|P-value:3.34E-8||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:REX2(YLR059C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:ROX3(YBL093C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL24B(YGR148C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM19(YNR037C)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RSM24(YDR175C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SCO1(YBR037C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SFC1(YJR095W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SHR5(YOL110W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SKG1(YKR100C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SLX9(YGR081C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SUL2(YLR092W)|FD-Score:5.53|P-value:1.57E-8||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUV3(YPL029W)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWF1(YDR126W)|FD-Score:9.29|P-value:7.69E-21||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SWI3(YJL176C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TCB2(YNL087W)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TEX1(YNL253W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TOM1(YDR457W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRI1(YMR233W)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:18.3|P-value:8.16E-75||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VID22(YLR373C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBR013C(YBR013C_p)|FD-Score:4.5|P-value:3.32E-6||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR197C(YBR197C_p)|FD-Score:-4.2|P-value:1.36E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCL012C(YCL012C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YGR125W(YGR125W_p)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR050C(YLR050C_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YLR149C(YLR149C_p)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR271W(YLR271W_p)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR400W(YLR400W_d)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML002W(YML002W_p)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML096W(YML096W_p)|FD-Score:-3.75|P-value:9.02E-5||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YNL203C(YNL203C_d)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR025C(YNR025C_d)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR228C(YOR228C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPR063C(YPR063C_p)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:ER-localized protein of unknown function Gene:YPR123C(YPR123C_d)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR147C(YPR147C_p)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:ABF2(YMR072W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ABP140(YOR239W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ALD5(YER073W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:ALF1(YNL148C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APT2(YDR441C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ATG7(YHR171W)|FD-Score:6.56|P-value:2.68E-11||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP14(YLR295C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP15(YPL271W)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ATP5(YDR298C)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:AUS1(YOR011W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BDS1(YOL164W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:CAR2(YLR438W)|FD-Score:-3.11|P-value:9.30E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CCS1(YMR038C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN5(YOR028C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CUE5(YOR042W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUP9(YPL177C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DCW1(YKL046C)|FD-Score:-3.89|P-value:5.09E-5||SGD DESC:Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p Gene:DIA4(YHR011W)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DOG2(YHR043C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:ECM32(YER176W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ECM8(YBR076W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Non-essential protein of unknown function Gene:FIG4(YNL325C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FMP23(YBR047W_p)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV6(YNL133C)|FD-Score:-5.3|P-value:5.89E-8||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GGC1(YDL198C)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GLC8(YMR311C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HCS1(YKL017C)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HHF1(YBR009C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HST2(YPL015C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IRE1(YHR079C)|FD-Score:-3.96|P-value:3.79E-5||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LDB18(YLL049W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:MDM1(YML104C)|FD-Score:-5.87|P-value:2.18E-9||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MET12(YPL023C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MRPL49(YJL096W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS17(YMR188C)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC6(YOR354C)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MSF1(YPR047W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MST1(YKL194C)|FD-Score:4.29|P-value:8.83E-6||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTC6(YHR151C)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NDE1(YMR145C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NSI1(YDR026C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication Gene:OAR1(YKL055C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OSW1(YOR255W)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PBP1(YGR178C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PEP7(YDR323C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PIF1(YML061C)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RER1(YCL001W)|FD-Score:-5.4|P-value:3.34E-8||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:REX2(YLR059C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:ROX3(YBL093C)|FD-Score:-4.25|P-value:1.05E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL24B(YGR148C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM19(YNR037C)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RSM24(YDR175C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SCO1(YBR037C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SFC1(YJR095W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SHR5(YOL110W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SKG1(YKR100C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SLX9(YGR081C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SUL2(YLR092W)|FD-Score:5.53|P-value:1.57E-8||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUV3(YPL029W)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWF1(YDR126W)|FD-Score:9.29|P-value:7.69E-21||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SWI3(YJL176C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TCB2(YNL087W)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TEX1(YNL253W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TOM1(YDR457W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRI1(YMR233W)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:18.3|P-value:8.16E-75||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VID22(YLR373C)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBR013C(YBR013C_p)|FD-Score:4.5|P-value:3.32E-6||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR197C(YBR197C_p)|FD-Score:-4.2|P-value:1.36E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YCL012C(YCL012C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YGR125W(YGR125W_p)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLR050C(YLR050C_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YLR149C(YLR149C_p)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR271W(YLR271W_p)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR400W(YLR400W_d)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML002W(YML002W_p)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML096W(YML096W_p)|FD-Score:-3.75|P-value:9.02E-5||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YNL203C(YNL203C_d)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR025C(YNR025C_d)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR228C(YOR228C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPR063C(YPR063C_p)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:ER-localized protein of unknown function Gene:YPR123C(YPR123C_d)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR147C(YPR147C_p)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C8.546.87E-182.15RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YBR136W6.398.28E-110.31MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YOR207C6.086.13E-101.51RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDL150W4.562.52E-60.27RPC53RNA polymerase III subunit C53
YBR002C4.298.79E-60.17RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YGR095C4.131.83E-50.24RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YOR063W3.895.11E-50.10RPL3Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus
YKL088W3.797.64E-50.26CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YPR142C_d3.532.09E-40.13YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YKR063C3.403.41E-40.20LAS1Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
YDL152W_d3.196.99E-40.04YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YNL161W3.167.94E-40.04CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YJR065C3.129.18E-40.03ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YJR064W3.080.001020.05CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR078C3.030.001220.02BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A18.308.16E-75TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR126W9.297.69E-21SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YHR171W6.562.68E-11ATG7Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation
YHR011W5.822.99E-9DIA4Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth
YLR092W5.531.57E-8SUL2High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
YHR151C5.462.42E-8MTC6Protein of unknown function; mtc6 is synthetically sick with cdc13-1
YNR037C5.141.35E-7RSM19Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein
YDL198C4.523.05E-6GGC1Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family
YBR013C_p4.503.32E-6YBR013C_pPutative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
YPR123C_d4.444.51E-6YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YBR047W_p4.424.91E-6FMP23_pPutative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YKL194C4.298.83E-6MST1Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro
YLR149C_p4.231.19E-5YLR149C_pProtein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
YMR145C4.221.23E-5NDE1Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain
YPL015C4.112.02E-5HST2Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export

GO enrichment analysis for SGTC_2113
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4144.48E-243SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.363636TSC3-RPN4
0.3857.41E-208SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0877193TSC3-RPN4
0.3751.77E-196SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.084507TSC3-RPN4
0.3722.49E-193SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.375TSC3-RPN4
0.3444.10E-164SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0714286
0.3363.50E-156SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0508475TSC3-RPN4
0.3353.14E-155SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0606061TSC3-RPN4
0.3282.82E-148SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0655738TSC3-RPN4
0.3063.29E-128SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.386364TSC3-RPN4
0.3043.17E-126SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.101695
0.2976.14E-121SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0526316TSC3-RPN4
0.2942.45E-118SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0862069TSC3-RPN4
0.2944.53E-118SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0461538TSC3-RPN4
0.2931.20E-117SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.230769TSC3-RPN4
0.2928.39E-117SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.16TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20865274770176.62 μM0.571429689278Chembridge (Fragment library)254.31031.46325
SGTC_20795212404121 μM0.5758462Chembridge (Fragment library)220.294081.34325
SGTC_2074521200775.4 μM0.475758611Chembridge (Fragment library)272.3007631.66826
SGTC_20775212015200 μM0.475780900Chembridge (Fragment library)288.755362.12725
SGTC_21115486399200 μM0.463415689674Chembridge (Fragment library)282.363462.40525
SGTC_20725231554129 μM0.441176789545Chembridge (Fragment library)192.240920.43125
SGTC_2071523095139.92 μM0.4390241799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4
SGTC_20785212016200 μM0.4251899708Chembridge (Fragment library)288.755362.1272560S ribosome export
SGTC_20735212005126 μM0.418605691789Chembridge (Fragment library)288.755362.12725
SGTC_20755212009200 μM0.418605780898Chembridge (Fragment library)272.3007631.66826