5521686

N-(2,5-dimethylphenyl)pyrazine-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2117
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 803312
SMILES CC1=CC(=C(C=C1)C)NC(=O)C2=NC=CN=C2
Standardized SMILES Cc1ccc(C)c(NC(=O)c2cnccn2)c1
Molecular weight 227.2618
ALogP 1.71
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.53
% growth inhibition (Hom. pool) 3.57


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 803312
Download HIP data (tab-delimited text)  (excel)
Gene:CAB2(YIL083C)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.19||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:LSM3(YLR438C-A)|FD-Score:3.18|P-value:7.35E-4|Clearance:0.15||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MET4(YNL103W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:PRE1(YER012W)|FD-Score:3.19|P-value:7.18E-4|Clearance:0.01||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RSE1(YML049C)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.26||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEN1(YLR430W)|FD-Score:4.14|P-value:1.71E-5|Clearance:0.48||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SEN34(YAR008W)|FD-Score:4.9|P-value:4.77E-7|Clearance:0.66||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:TEM1(YML064C)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.1||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:TIF35(YDR429C)|FD-Score:-3.39|P-value:3.45E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM22(YNL131W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YBR124W(YBR124W_d)|FD-Score:5.49|P-value:2.00E-8|Clearance:0.66||SGD DESC:Putative protein of unknown function Gene:CAB2(YIL083C)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.19||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:LSM3(YLR438C-A)|FD-Score:3.18|P-value:7.35E-4|Clearance:0.15||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MET4(YNL103W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:PRE1(YER012W)|FD-Score:3.19|P-value:7.18E-4|Clearance:0.01||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RSE1(YML049C)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.26||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEN1(YLR430W)|FD-Score:4.14|P-value:1.71E-5|Clearance:0.48||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SEN34(YAR008W)|FD-Score:4.9|P-value:4.77E-7|Clearance:0.66||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:TEM1(YML064C)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.1||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:TIF35(YDR429C)|FD-Score:-3.39|P-value:3.45E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM22(YNL131W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YBR124W(YBR124W_d)|FD-Score:5.49|P-value:2.00E-8|Clearance:0.66||SGD DESC:Putative protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 803312
Download HOP data (tab-delimited text)  (excel)
Gene:ANP1(YEL036C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APS1(YLR170C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARO1(YDR127W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG1(YGL180W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:AVT2(YEL064C)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAC2(YML102W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CAF16(YFL028C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:COX14(YML129C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:COX17(YLL009C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CYT2(YKL087C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DGA1(YOR245C)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DOC1(YGL240W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:ECM32(YER176W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:EDS1(YBR033W_p)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMC6(YLL014W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:EMP24(YGL200C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:FAU1(YER183C)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GAP1(YKR039W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:HPM1(YIL110W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HXK1(YFR053C)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:INM2(YDR287W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:IRE1(YHR079C)|FD-Score:-3.59|P-value:1.62E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:ITR1(YDR497C)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:NHP6B(YBR089C-A)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication Gene:PDH1(YPR002W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PEX17(YNL214W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PRB1(YEL060C)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PRM7(YDL039C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:PXA2(YKL188C)|FD-Score:5.71|P-value:5.78E-9||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RAV2(YDR202C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RFM1(YOR279C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPL21B(YPL079W)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL26A(YLR344W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.73|P-value:9.48E-5||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPS11A(YDR025W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RRT7(YLL030C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:RSA1(YPL193W)|FD-Score:4.88|P-value:5.31E-7||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SAS5(YOR213C)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SCY1(YGL083W)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SFA1(YDL168W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHB17(YKR043C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHR5(YOL110W)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SNT309(YPR101W)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SNX41(YDR425W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p Gene:SPR1(YOR190W)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:TEL1(YBL088C)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TIS11(YLR136C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URM1(YIL008W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VMA8(YEL051W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS41(YDR080W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YBL029W(YBL029W_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Non-essential protein of unknown function Gene:YBR174C(YBR174C_d)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YDR010C(YDR010C_d)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL010W(YGL010W_p)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL015C(YGL015C_p)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGR176W(YGR176W_d)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR237C(YGR237C_p)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIL028W(YIL028W_d)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:6.8|P-value:5.17E-12||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YJL181W(YJL181W_p)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YJL215C(YJL215C_d)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL020C(YLL020C_d)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR225C(YLR225C_p)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YMR141C(YMR141C_d)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR158W-B(YMR158W-B_d)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YMR196W(YMR196W_p)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR309C(YOR309C_d)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:ANP1(YEL036C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APS1(YLR170C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARO1(YDR127W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG1(YGL180W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:AVT2(YEL064C)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAC2(YML102W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CAF16(YFL028C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:COX14(YML129C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:COX17(YLL009C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CYT2(YKL087C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DGA1(YOR245C)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DOC1(YGL240W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:ECM32(YER176W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:EDS1(YBR033W_p)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMC6(YLL014W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:EMP24(YGL200C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:FAU1(YER183C)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GAP1(YKR039W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:HPM1(YIL110W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HXK1(YFR053C)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:INM2(YDR287W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:IRE1(YHR079C)|FD-Score:-3.59|P-value:1.62E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:ITR1(YDR497C)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:NHP6B(YBR089C-A)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication Gene:PDH1(YPR002W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PEX17(YNL214W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PRB1(YEL060C)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PRM7(YDL039C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:PXA2(YKL188C)|FD-Score:5.71|P-value:5.78E-9||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RAV2(YDR202C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RFM1(YOR279C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPL21B(YPL079W)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL26A(YLR344W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.73|P-value:9.48E-5||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPS11A(YDR025W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RRT7(YLL030C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:RSA1(YPL193W)|FD-Score:4.88|P-value:5.31E-7||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SAS5(YOR213C)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SCY1(YGL083W)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SFA1(YDL168W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHB17(YKR043C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHR5(YOL110W)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SNT309(YPR101W)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SNX41(YDR425W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p Gene:SPR1(YOR190W)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:TEL1(YBL088C)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TIS11(YLR136C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URM1(YIL008W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VMA8(YEL051W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS41(YDR080W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YBL029W(YBL029W_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Non-essential protein of unknown function Gene:YBR174C(YBR174C_d)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YDR010C(YDR010C_d)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL010W(YGL010W_p)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL015C(YGL015C_p)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGR176W(YGR176W_d)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR237C(YGR237C_p)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIL028W(YIL028W_d)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:6.8|P-value:5.17E-12||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YJL181W(YJL181W_p)|FD-Score:-3.97|P-value:3.62E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YJL215C(YJL215C_d)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL020C(YLL020C_d)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR225C(YLR225C_p)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YMR141C(YMR141C_d)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR158W-B(YMR158W-B_d)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YMR196W(YMR196W_p)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR309C(YOR309C_d)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR124W_d5.492.00E-80.66YBR124W_dPutative protein of unknown function
YAR008W4.904.77E-70.66SEN34Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
YML064C4.241.10E-50.10TEM1GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis
YLR430W4.141.71E-50.48SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YIL083C3.671.22E-40.18CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YNL131W3.482.48E-40.03TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YML049C3.452.79E-40.27RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
YER012W3.197.18E-40.01PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YLR438C-A3.187.35E-40.15LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YOL130W3.030.001210.04ALR1Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
YLR249W2.990.001390.03YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YDL014W2.960.001520.10NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YJR046W2.860.002120.01TAH11DNA replication licensing factor, required for pre-replication complex assembly
YNL007C2.850.002190.07SIS1Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
YPL228W2.780.002750.02CET1Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL054W_p6.805.17E-12YIL054W_pProtein of unknown function; expressed at both mRNA and protein levels
YKL188C5.715.78E-9PXA2Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder
YOR213C5.531.56E-8SAS5Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
YGL015C_p5.081.93E-7YGL015C_pPutative protein of unknown function; null mutants accumulate cargo in the Golgi
YPL193W4.885.31E-7RSA1Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
YFR053C4.817.52E-7HXK1Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
YIL028W_d4.641.70E-6YIL028W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YNR025C_d4.356.75E-6YNR025C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase
YLL009C4.241.10E-5COX17Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
YDR287W3.983.40E-5INM2Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
YKL087C3.944.01E-5CYT2Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
YEL064C3.934.19E-5AVT2Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YBL088C3.924.39E-5TEL1Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
YGR237C_p3.885.16E-5YGR237C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YML102W3.865.65E-5CAC2Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription

GO enrichment analysis for SGTC_2117
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0985.15E-14SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.03125TSC3-RPN4
0.0857.64E-11SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0967742TSC3-RPN4
0.0806.90E-10SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0819672TSC3-RPN4
0.0758.25E-9SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.175439
0.0741.23E-8SGTC_10420350-0096 82.0 μMChemDiv (Drug-like library)31225150.118644TSC3-RPN4
0.0732.43E-8SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.126984TSC3-RPN4
0.0713.79E-8SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.140845
0.0714.15E-8SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0857143TSC3-RPN4
0.0714.98E-8SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.135593TSC3-RPN4
0.0715.46E-8SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.114754TSC3-RPN4
0.0706.19E-8SGTC_1726st035556 14.6 μMTimTec (Natural product derivative library)59194390.1
0.0706.47E-8SGTC_21325317594 122.7 μMChembridge (Fragment library)7945770.0597015SWF1 & branched chain AA biosynthesis
0.0708.58E-8SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.112903TSC3-RPN4
0.0681.76E-7SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.0166667TSC3-RPN4
0.0672.36E-7SGTC_1891st059073 26.2 μMTimTec (Natural product derivative library)6887540.0862069TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21505556613200 μM0.627907743190Chembridge (Fragment library)213.235241.22813
SGTC_22057238770200 μM0.545455279867Chembridge (Fragment library)278.104721.4913
SGTC_21545570129200 μM0.543478673366Chembridge (Fragment library)227.261821.68413
SGTC_21876125751200 μM0.5874498Chembridge (Fragment library)235.1895861.15215RSC complex & mRNA processing
SGTC_21185526350200 μM0.367347743594Chembridge (Fragment library)212.290243.25912
SGTC_3228913179949.47 μM0.31666717015818Chembridge (Drug-like library)307.349783.15224amide catabolism
SGTC_22757947463200 μM0.3148151225414Chembridge (Fragment library)216.235880.85813iron homeostasis
SGTC_3082911700849.47 μM0.31481541448529Chembridge (Drug-like library)240.300342.44112
SGTC_21455634607200 μM0.313726308662Chembridge (Fragment library)231.250522.02733
SGTC_3269913782349.47 μM0.31147517682936Chembridge (Drug-like library)309.38552.9041460S ribosome export