5528445

3,4-dimethyl-N-(pyridin-3-ylmethyl)aniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2119
Screen concentration 120.6 μM
Source Chembridge (Fragment library)
PubChem CID 791935
SMILES CC1=C(C=C(C=C1)NCC2=CN=CC=C2)C
Standardized SMILES Cc1ccc(NCc2cccnc2)cc1C
Molecular weight 212.2902
ALogP 3.04
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.53
% growth inhibition (Hom. pool) 8.75


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 791935
Download HIP data (tab-delimited text)  (excel)
Gene:BRL1(YHR036W)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CAB4(YGR277C)|FD-Score:3.34|P-value:4.25E-4|Clearance:0.05||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:CDC25(YLR310C)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.04||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CWC2(YDL209C)|FD-Score:3.29|P-value:5.10E-4|Clearance:0.09||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DHR2(YKL078W)|FD-Score:3.4|P-value:3.31E-4|Clearance:0.07||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DPB2(YPR175W)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.03||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:ECM16(YMR128W)|FD-Score:3.77|P-value:8.32E-5|Clearance:0.28||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:MCM10(YIL150C)|FD-Score:4.45|P-value:4.35E-6|Clearance:0.37||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:RPC10(YHR143W-A)|FD-Score:-3.24|P-value:5.92E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPN5(YDL147W)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RRP46(YGR095C)|FD-Score:-3.19|P-value:7.13E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:TAF12(YDR145W)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.37||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF35(YDR429C)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.37||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM10(YHR005C-A)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.11||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YDR396W(YDR396W_d)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YTM1(YOR272W)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:BRL1(YHR036W)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CAB4(YGR277C)|FD-Score:3.34|P-value:4.25E-4|Clearance:0.05||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:CDC25(YLR310C)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.04||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CWC2(YDL209C)|FD-Score:3.29|P-value:5.10E-4|Clearance:0.09||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DHR2(YKL078W)|FD-Score:3.4|P-value:3.31E-4|Clearance:0.07||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DPB2(YPR175W)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.03||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:ECM16(YMR128W)|FD-Score:3.77|P-value:8.32E-5|Clearance:0.28||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:MCM10(YIL150C)|FD-Score:4.45|P-value:4.35E-6|Clearance:0.37||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:RPC10(YHR143W-A)|FD-Score:-3.24|P-value:5.92E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPN5(YDL147W)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RRP46(YGR095C)|FD-Score:-3.19|P-value:7.13E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:TAF12(YDR145W)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.37||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF35(YDR429C)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.37||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM10(YHR005C-A)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.11||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YDR396W(YDR396W_d)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:YTM1(YOR272W)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 791935
Download HOP data (tab-delimited text)  (excel)
Gene:AIM29(YKR074W)|FD-Score:-4.84|P-value:6.36E-7||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARE1(YCR048W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:BST1(YFL025C)|FD-Score:5.33|P-value:4.83E-8||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:COG7(YGL005C)|FD-Score:-4.3|P-value:8.68E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRF1(YDR223W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:CRH1(YGR189C)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CYC7(YEL039C)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DID4(YKL002W)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:ECM22(YLR228C)|FD-Score:6.77|P-value:6.33E-12||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:EFM2(YBR271W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ENV10(YLR065C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERI1(YPL096C-A)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FET3(YMR058W)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:FMP40(YPL222W_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAS2(YLR343W)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GEX2(YKR106W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:HXT12(YIL170W)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:IRC14(YOR135C_d)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:LSC1(YOR142W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MCX1(YBR227C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MET10(YFR030W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MFM1(YPL060W)|FD-Score:-4.27|P-value:9.82E-6||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MHP1(YJL042W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MLC2(YPR188C)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MNE1(YOR350C)|FD-Score:4.85|P-value:6.08E-7||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MNT3(YIL014W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MON2(YNL297C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MPD1(YOR288C)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MSG5(YNL053W)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:OPI6(YDL096C_d)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:ORM1(YGR038W)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAR32(YDL173W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEP12(YOR036W)|FD-Score:-4.78|P-value:8.93E-7||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX30(YLR324W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHM6(YDR281C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PML39(YML107C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PRO2(YOR323C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RMD8(YFR048W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPA14(YDR156W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPN4(YDL020C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:-4.67|P-value:1.53E-6||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRT2(YBR246W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SAS5(YOR213C)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SIP3(YNL257C)|FD-Score:5.81|P-value:3.18E-9||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLF1(YDR515W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPE3(YPR069C)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPG1(YGR236C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SUR4(YLR372W)|FD-Score:5.82|P-value:2.95E-9||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWA2(YDR320C)|FD-Score:-6.67|P-value:1.30E-11||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TDA10(YGR205W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA2(YER071C_p)|FD-Score:5.36|P-value:4.09E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TLG2(YOL018C)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRM10(YOL093W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRS33(YOR115C)|FD-Score:-4.2|P-value:1.36E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UGA1(YGR019W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress Gene:XKS1(YGR194C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains Gene:YAR028W(YAR028W_p)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBL012C(YBL012C_d)|FD-Score:-4.31|P-value:8.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR249C(YDR249C_p)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Putative protein of unknown function Gene:YER181C(YER181C_d)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YFL063W(YFL063W_d)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Dubious open reading frame, based on available experimental and comparative sequence data Gene:YGL138C(YGL138C_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YIL024C(YIL024C_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YJL135W(YJL135W_d)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YKR073C(YKR073C_d)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR253W(YLR253W_p)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YML096W(YML096W_p)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YML122C(YML122C_d)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL163W(YOL163W_p)|FD-Score:4.94|P-value:3.92E-7||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YPR197C(YPR197C_d)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AIM29(YKR074W)|FD-Score:-4.84|P-value:6.36E-7||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARE1(YCR048W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:BST1(YFL025C)|FD-Score:5.33|P-value:4.83E-8||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:COG7(YGL005C)|FD-Score:-4.3|P-value:8.68E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRF1(YDR223W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:CRH1(YGR189C)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CYC7(YEL039C)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DID4(YKL002W)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:ECM22(YLR228C)|FD-Score:6.77|P-value:6.33E-12||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:EFM2(YBR271W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ENV10(YLR065C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERI1(YPL096C-A)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FET3(YMR058W)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:FMP40(YPL222W_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAS2(YLR343W)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GEX2(YKR106W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:HXT12(YIL170W)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:IRC14(YOR135C_d)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:LSC1(YOR142W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MCX1(YBR227C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MET10(YFR030W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MFM1(YPL060W)|FD-Score:-4.27|P-value:9.82E-6||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MHP1(YJL042W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MLC2(YPR188C)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MNE1(YOR350C)|FD-Score:4.85|P-value:6.08E-7||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MNT3(YIL014W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MON2(YNL297C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MPD1(YOR288C)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MSG5(YNL053W)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:OPI6(YDL096C_d)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:ORM1(YGR038W)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAR32(YDL173W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEP12(YOR036W)|FD-Score:-4.78|P-value:8.93E-7||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX30(YLR324W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHM6(YDR281C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PML39(YML107C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PRO2(YOR323C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RMD8(YFR048W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RPA14(YDR156W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPN4(YDL020C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:-4.67|P-value:1.53E-6||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RRT2(YBR246W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SAS5(YOR213C)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SIP3(YNL257C)|FD-Score:5.81|P-value:3.18E-9||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLF1(YDR515W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPE3(YPR069C)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPG1(YGR236C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SUR4(YLR372W)|FD-Score:5.82|P-value:2.95E-9||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWA2(YDR320C)|FD-Score:-6.67|P-value:1.30E-11||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TDA10(YGR205W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA2(YER071C_p)|FD-Score:5.36|P-value:4.09E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TLG2(YOL018C)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRM10(YOL093W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRS33(YOR115C)|FD-Score:-4.2|P-value:1.36E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UGA1(YGR019W)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress Gene:XKS1(YGR194C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains Gene:YAR028W(YAR028W_p)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBL012C(YBL012C_d)|FD-Score:-4.31|P-value:8.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR249C(YDR249C_p)|FD-Score:3.83|P-value:6.31E-5||SGD DESC:Putative protein of unknown function Gene:YER181C(YER181C_d)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YFL063W(YFL063W_d)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Dubious open reading frame, based on available experimental and comparative sequence data Gene:YGL138C(YGL138C_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YIL024C(YIL024C_p)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YJL135W(YJL135W_d)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YKR073C(YKR073C_d)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR253W(YLR253W_p)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YML096W(YML096W_p)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YML122C(YML122C_d)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL163W(YOL163W_p)|FD-Score:4.94|P-value:3.92E-7||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YPR197C(YPR197C_d)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YIL150C4.454.35E-60.37MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YDR429C4.241.12E-50.37TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YDR145W4.131.79E-50.37TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YMR128W3.778.32E-50.28ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YDR396W_d3.482.49E-40.08YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YKL078W3.403.31E-40.07DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YGR277C3.344.25E-40.05CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YDL209C3.295.10E-40.09CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YPR175W3.197.10E-40.03DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YLR310C3.167.83E-40.04CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YOR272W3.138.89E-40.03YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YHR005C-A3.109.76E-40.11TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YML077W2.990.001400.02BET5Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
YHR062C2.970.001500.07RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR072W2.900.001870.00ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YLR228C6.776.33E-12ECM22Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
YLR372W5.822.95E-9SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YNL257C5.813.18E-9SIP3Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
YER071C_p5.364.09E-8TDA2_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele
YFL025C5.334.83E-8BST1GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
YOL163W_p4.943.92E-7YOL163W_pPutative protein of unknown function; member of the Dal5p subfamily of the major facilitator family
YOR350C4.856.08E-7MNE1Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA
YOR323C4.464.03E-6PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YLR343W4.415.16E-6GAS21,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p
YIL170W4.395.57E-6HXT12Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
YPL096C-A4.131.78E-5ERI1Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein
YGR205W4.111.99E-5TDA10ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele
YDL173W4.102.03E-5PAR32Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene
YFR030W4.072.37E-5MET10Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide
YDR515W4.032.77E-5SLF1RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2119
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0944.03E-13SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.0923077
0.0872.29E-11SGTC_31439086658 49.5 μMChembridge (Drug-like library)252366260.114754
0.0781.71E-9SGTC_3451362-0200 395.0 μMChemDiv (Drug-like library)14198230.140351
0.0715.68E-8SGTC_2551apiole 73.9 μMMicrosource (Natural product library)106590.0833333
0.0681.85E-7SGTC_22927671323 168.1 μMChembridge (Fragment library)9375040.0952381RNA processing & uracil transport
0.0664.49E-7SGTC_31999111318 49.5 μMChembridge (Drug-like library)85480200.132353
0.0588.49E-6SGTC_14103909-8106 77.9 μMChemDiv (Drug-like library)10392100.152778
0.0571.15E-5SGTC_29047225135 42.0 μMChembridge (Drug-like library)44461160.112676
0.0571.36E-5SGTC_1545lovastatin 49.4 μMTimTec (Pure natural product library)129236120.0454545
0.0561.80E-5SGTC_22127123476 200.0 μMChembridge (Fragment library)10857150.111111
0.0561.85E-5SGTC_12630774-1208 64.8 μMChemDiv (Drug-like library)9065850.0806452
0.0552.49E-5SGTC_20464110841 106.0 μMChembridge (Fragment library)441196300.122449iron homeostasis
0.0552.72E-5SGTC_2934049-0273 38.5 μMChemDiv (Drug-like library)13803110.118644
0.0542.88E-5SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene 92.6 μMMicrosource (Natural product library)67087600.0833333
0.0543.36E-5SGTC_13331482-0047 286.0 μMChemDiv (Drug-like library)38111160.0166667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20975376186200 μM0.694444742755Chembridge (Fragment library)198.263662.55712
SGTC_2138557559377.67 μM0.666667762213Chembridge (Fragment library)212.290243.04412
SGTC_3338914430039.57 μM0.4035094901274Chembridge (Drug-like library)343.424943.45314Golgi
SGTC_3342914779114.51 μM0.3965521838168Chembridge (Drug-like library)349.816843.34914
SGTC_334491488623.9 μM0.3965524905352Chembridge (Drug-like library)349.816843.34914
SGTC_3351915031221.6 μM0.3928574904905Chembridge (Drug-like library)329.398363.17114cell wall
SGTC_3321913779326.83 μM0.389834904192Chembridge (Drug-like library)343.424943.63414cell wall
SGTC_3291912117771.43 μM0.38333317015612Chembridge (Drug-like library)341.794842.95414
SGTC_335691518114.48 μM0.3770491859254Chembridge (Drug-like library)357.451524.0914cell wall
SGTC_2830799200771.43 μM0.3751952075Chembridge (Drug-like library)364.752194.79517