5251174

2,6-diamino-4H-thiopyran-3,5-dicarbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2126
Screen concentration 33.9 μM
Source Chembridge (Fragment library)
PubChem CID 790722
SMILES C1C(=C(SC(=C1C#N)N)N)C#N
Standardized SMILES NC1=C(CC(=C(N)S1)C#N)C#N
Molecular weight 178.2143
ALogP 0.18
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.5
% growth inhibition (Hom. pool) 8.44


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 790722
Download HIP data (tab-delimited text)  (excel)
Gene:CDC123(YLR215C)|FD-Score:-4.37|P-value:6.11E-6|Clearance:0||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:CDC4(YFL009W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.02||SGD DESC:F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p Gene:FAS2(YPL231W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.07||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:FOL3(YMR113W)|FD-Score:-4.26|P-value:1.04E-5|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HRP1(YOL123W)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:ISD11(YER048W-A)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.25||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LSM5(YER146W)|FD-Score:-3.72|P-value:9.79E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NOP53(YPL146C)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.38||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:NUP82(YJL061W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.02||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PIK1(YNL267W)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PSA1(YDL055C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:PXR1(YGR280C)|FD-Score:-3.64|P-value:1.39E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain Gene:RPB5(YBR154C)|FD-Score:6.69|P-value:1.14E-11|Clearance:0.93||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL15A(YLR029C)|FD-Score:3.17|P-value:7.60E-4|Clearance:0.3||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL18A(YOL120C)|FD-Score:4.06|P-value:2.40E-5|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRP9(YPR137W)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.01||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SMX2(YFL017W-A)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.04||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SRB7(YDR308C)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.04||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:SUI3(YPL237W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.05||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TFG2(YGR005C)|FD-Score:4.98|P-value:3.25E-7|Clearance:0.75||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TIF35(YDR429C)|FD-Score:5.94|P-value:1.40E-9|Clearance:0.93||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TUB2(YFL037W)|FD-Score:-4.45|P-value:4.23E-6|Clearance:0||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:WRS1(YOL097C)|FD-Score:3.17|P-value:7.56E-4|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YDL196W(YDL196W_d)|FD-Score:-3.39|P-value:3.56E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR413C(YDR413C_d)|FD-Score:5.01|P-value:2.73E-7|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YPL238C(YPL238C_d)|FD-Score:7.33|P-value:1.15E-13|Clearance:0.93||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPR136C(YPR136C_d)|FD-Score:4.23|P-value:1.17E-5|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:CDC123(YLR215C)|FD-Score:-4.37|P-value:6.11E-6|Clearance:0||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:CDC4(YFL009W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.02||SGD DESC:F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p Gene:FAS2(YPL231W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.07||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:FOL3(YMR113W)|FD-Score:-4.26|P-value:1.04E-5|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HRP1(YOL123W)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:ISD11(YER048W-A)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.25||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LSM5(YER146W)|FD-Score:-3.72|P-value:9.79E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NOP53(YPL146C)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.38||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:NUP82(YJL061W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.02||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PIK1(YNL267W)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PSA1(YDL055C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:PXR1(YGR280C)|FD-Score:-3.64|P-value:1.39E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain Gene:RPB5(YBR154C)|FD-Score:6.69|P-value:1.14E-11|Clearance:0.93||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL15A(YLR029C)|FD-Score:3.17|P-value:7.60E-4|Clearance:0.3||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL18A(YOL120C)|FD-Score:4.06|P-value:2.40E-5|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRP9(YPR137W)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.01||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SMX2(YFL017W-A)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.04||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SRB7(YDR308C)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.04||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:SUI3(YPL237W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.05||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TFG2(YGR005C)|FD-Score:4.98|P-value:3.25E-7|Clearance:0.75||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TIF35(YDR429C)|FD-Score:5.94|P-value:1.40E-9|Clearance:0.93||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TUB2(YFL037W)|FD-Score:-4.45|P-value:4.23E-6|Clearance:0||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:WRS1(YOL097C)|FD-Score:3.17|P-value:7.56E-4|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YDL196W(YDL196W_d)|FD-Score:-3.39|P-value:3.56E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR413C(YDR413C_d)|FD-Score:5.01|P-value:2.73E-7|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YPL238C(YPL238C_d)|FD-Score:7.33|P-value:1.15E-13|Clearance:0.93||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPR136C(YPR136C_d)|FD-Score:4.23|P-value:1.17E-5|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 790722
Download HOP data (tab-delimited text)  (excel)
Gene:AAD6(YFL056C)|FD-Score:3.81|P-value:6.81E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:AGP3(YFL055W)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:AIM13(YFR011C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD4(YOR374W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:ATG32(YIL146C)|FD-Score:5.75|P-value:4.35E-9||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATG34(YOL083W)|FD-Score:4.99|P-value:2.95E-7||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:BNA2(YJR078W)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:BOR1(YNL275W)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:CCE1(YKL011C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CCS1(YMR038C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN8(YEL061C)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CLB6(YGR109C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CMK1(YFR014C)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:-6.05|P-value:7.06E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR5(YDR304C)|FD-Score:4.39|P-value:5.56E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CUE5(YOR042W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUP2(YGL166W)|FD-Score:4.92|P-value:4.26E-7||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DAL80(YKR034W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DBF2(YGR092W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:EAP1(YKL204W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EMP47(YFL048C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ENO1(YGR254W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERG5(YMR015C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:GAD1(YMR250W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCN3(YKR026C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GLO2(YDR272W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GOS1(YHL031C)|FD-Score:-4.9|P-value:4.71E-7||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:HAP5(YOR358W)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HFM1(YGL251C)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HIR2(YOR038C)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p Gene:HXT2(YMR011W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IBA57(YJR122W)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IMG1(YCR046C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:INP52(YNL106C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain Gene:IRC3(YDR332W)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:ISA1(YLL027W)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:ISC10(YER180C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:ITT1(YML068W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:JLP2(YMR132C_p)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KRE28(YDR532C)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:KTR4(YBR199W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LSC2(YGR244C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:LST4(YKL176C)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MET6(YER091C)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:MGA2(YIR033W)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MGR3(YMR115W)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MPH1(YIR002C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MPP6(YNR024W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRS3(YJL133W)|FD-Score:4.57|P-value:2.40E-6||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MTQ1(YNL063W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NIT3(YLR351C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NNT1(YLR285W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NUR1(YDL089W)|FD-Score:-6.63|P-value:1.66E-11||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OPY2(YPR075C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PBP1(YGR178C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PBP4(YDL053C)|FD-Score:6.06|P-value:6.97E-10||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PDC5(YLR134W)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEX14(YGL153W)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PEX19(YDL065C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:POM33(YLL023C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress Gene:PPH21(YDL134C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PUF2(YPR042C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RAD30(YDR419W)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV Gene:RCF2(YNR018W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RGI1(YER067W)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:ROY1(YMR258C)|FD-Score:-5.58|P-value:1.20E-8||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL16B(YNL069C)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:SCS3(YGL126W)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SFB3(YHR098C)|FD-Score:-4.55|P-value:2.68E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHY1(YGR112W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome Gene:SNL1(YIL016W)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SOD1(YJR104C)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPH1(YLR313C)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SUM1(YDR310C)|FD-Score:5.59|P-value:1.14E-8||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SWF1(YDR126W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TAE1(YBR261C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:THI6(YPL214C)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TIM21(YGR033C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:UBP2(YOR124C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:UBP8(YMR223W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:UME1(YPL139C)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:URA2(YJL130C)|FD-Score:7.07|P-value:7.78E-13||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VMA1(YDL185W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA8(YEL051W)|FD-Score:-4.22|P-value:1.23E-5||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS61(YDR136C_d)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS69(YPR087W_d)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:WSC4(YHL028W)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YBR099C(YBR099C_d)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YDL118W(YDL118W_p)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL172C(YDL172C_d)|FD-Score:3.8|P-value:7.24E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-4.06|P-value:2.40E-5||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YEL008W(YEL008W_d)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YEL045C(YEL045C_d)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Putative protein of unknown function Gene:YGR146C-A(YGR146C-A_p)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Putative protein of unknown function Gene:YGR174W-A(YGR174W-A_p)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YHR180W(YHR180W_d)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL064W(YJL064W_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YJR015W(YJR015W_p)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YLR031W(YLR031W_p)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Putative protein of unknown function Gene:YML079W(YML079W_p)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YML131W(YML131W_p)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YMR252C(YMR252C_p)|FD-Score:4|P-value:3.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YNL011C(YNL011C_p)|FD-Score:4.89|P-value:5.03E-7||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YNL324W(YNL324W_d)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR050C(YOR050C_d)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YOR072W(YOR072W_d)|FD-Score:3.74|P-value:9.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR161C-C(YOR161C-C_p)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPL071C(YPL071C_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL261C(YPL261C_d)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR059C(YPR059C_d)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR196W(YPR196W_p)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YSY6(YBR162W-A)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion Gene:ZWF1(YNL241C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AAD6(YFL056C)|FD-Score:3.81|P-value:6.81E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:AGP3(YFL055W)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:AIM13(YFR011C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD4(YOR374W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:ATG32(YIL146C)|FD-Score:5.75|P-value:4.35E-9||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATG34(YOL083W)|FD-Score:4.99|P-value:2.95E-7||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:BNA2(YJR078W)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:BOR1(YNL275W)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:CCE1(YKL011C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CCS1(YMR038C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN8(YEL061C)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CLB6(YGR109C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CMK1(YFR014C)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:-6.05|P-value:7.06E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR5(YDR304C)|FD-Score:4.39|P-value:5.56E-6||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CUE5(YOR042W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUP2(YGL166W)|FD-Score:4.92|P-value:4.26E-7||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DAL80(YKR034W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DBF2(YGR092W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:EAP1(YKL204W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EMP47(YFL048C)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ENO1(YGR254W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ERG5(YMR015C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:GAD1(YMR250W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCN3(YKR026C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GLO2(YDR272W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GOS1(YHL031C)|FD-Score:-4.9|P-value:4.71E-7||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:HAP5(YOR358W)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HFM1(YGL251C)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HIR2(YOR038C)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p Gene:HXT2(YMR011W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IBA57(YJR122W)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IMG1(YCR046C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:INP52(YNL106C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain Gene:IRC3(YDR332W)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:ISA1(YLL027W)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:ISC10(YER180C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:ITT1(YML068W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:JLP2(YMR132C_p)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KRE28(YDR532C)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:KTR4(YBR199W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family Gene:LSC2(YGR244C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:LST4(YKL176C)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MET6(YER091C)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:MGA2(YIR033W)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MGR3(YMR115W)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MPH1(YIR002C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MPP6(YNR024W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRS3(YJL133W)|FD-Score:4.57|P-value:2.40E-6||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MTQ1(YNL063W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NIT3(YLR351C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NNT1(YLR285W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NUR1(YDL089W)|FD-Score:-6.63|P-value:1.66E-11||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OPY2(YPR075C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PBP1(YGR178C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PBP4(YDL053C)|FD-Score:6.06|P-value:6.97E-10||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PDC5(YLR134W)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEX14(YGL153W)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PEX19(YDL065C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:POM33(YLL023C)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress Gene:PPH21(YDL134C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PUF2(YPR042C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RAD30(YDR419W)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV Gene:RCF2(YNR018W)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RGI1(YER067W)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:ROY1(YMR258C)|FD-Score:-5.58|P-value:1.20E-8||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL16B(YNL069C)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:SCS3(YGL126W)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SFB3(YHR098C)|FD-Score:-4.55|P-value:2.68E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SHY1(YGR112W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome Gene:SNL1(YIL016W)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SOD1(YJR104C)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPH1(YLR313C)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SUM1(YDR310C)|FD-Score:5.59|P-value:1.14E-8||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SWF1(YDR126W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TAE1(YBR261C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:THI6(YPL214C)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TIM21(YGR033C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:UBP2(YOR124C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:UBP8(YMR223W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:UME1(YPL139C)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:URA2(YJL130C)|FD-Score:7.07|P-value:7.78E-13||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VMA1(YDL185W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA8(YEL051W)|FD-Score:-4.22|P-value:1.23E-5||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS61(YDR136C_d)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS69(YPR087W_d)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:WSC4(YHL028W)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 Gene:YBR099C(YBR099C_d)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YDL118W(YDL118W_p)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL172C(YDL172C_d)|FD-Score:3.8|P-value:7.24E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-4.06|P-value:2.40E-5||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YEL008W(YEL008W_d)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YEL045C(YEL045C_d)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Putative protein of unknown function Gene:YGR146C-A(YGR146C-A_p)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Putative protein of unknown function Gene:YGR174W-A(YGR174W-A_p)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YHR180W(YHR180W_d)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL064W(YJL064W_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YJR015W(YJR015W_p)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YLR031W(YLR031W_p)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Putative protein of unknown function Gene:YML079W(YML079W_p)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YML131W(YML131W_p)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YMR252C(YMR252C_p)|FD-Score:4|P-value:3.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YNL011C(YNL011C_p)|FD-Score:4.89|P-value:5.03E-7||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YNL324W(YNL324W_d)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR050C(YOR050C_d)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Gene:YOR072W(YOR072W_d)|FD-Score:3.74|P-value:9.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR161C-C(YOR161C-C_p)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPL071C(YPL071C_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL261C(YPL261C_d)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR059C(YPR059C_d)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR196W(YPR196W_p)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YSY6(YBR162W-A)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion Gene:ZWF1(YNL241C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL238C_d7.331.15E-130.93YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YBR154C6.691.14E-110.93RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YDR429C5.941.40E-90.93TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YDR413C_d5.012.73E-70.03YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YGR005C4.983.25E-70.75TFG2TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
YPR136C_d4.231.17E-50.17YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YOL120C4.062.40E-50.02RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YPL146C4.052.61E-50.38NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YDL055C3.661.24E-44.63E-4PSA1GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
YJL061W3.661.24E-40.02NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YDR308C3.641.34E-40.04SRB7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
YPL237W3.611.54E-40.05SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YFL009W3.561.84E-40.02CDC4F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
YPR137W3.551.96E-40.01RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YPL231W3.532.08E-40.07FAS2Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL130C7.077.78E-13URA2Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
YDL053C6.066.97E-10PBP4Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
YIL146C5.754.35E-9ATG32Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane
YDR310C5.591.14E-8SUM1Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
YPL214C5.591.16E-8THI6Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YHL028W5.161.21E-7WSC4ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
YEL008W_d5.111.60E-7YEL008W_dHypothetical protein predicted to be involved in metabolism
YOL083W4.992.95E-7ATG34Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog
YGL166W4.924.26E-7CUP2Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
YNL011C_p4.895.03E-7YNL011C_pPutative protein of unknown function; YNL011C is not an essential gene
YOR358W4.808.13E-7HAP5Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
YOR050C_d4.798.32E-7YOR050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
YNL063W4.681.46E-6MTQ1S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene
YMR011W4.661.59E-6HXT2High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose
YKR026C4.582.36E-6GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression

GO enrichment analysis for SGTC_2126
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0663.95E-7SGTC_23589037157 4.0 μMChembridge (Fragment library)23889250.04
0.0655.47E-7SGTC_15427-hydroxy-3'-methoxyflavone 49.7 μMTimTec (Pure natural product library)53931530.0175439
0.0621.96E-6SGTC_32329132874 49.5 μMChembridge (Drug-like library)37849400.0208333RPP1 & pyrimidine depletion
0.0603.77E-6SGTC_20245144227 134.0 μMChembridge (Fragment library)34752110.0384615
0.0604.56E-6SGTC_2518prenyletin 46.3 μMMicrosource (Natural product library)38734590.0178571
0.0552.10E-5SGTC_169k015-0017 16.0 μMChemDiv (Drug-like library)18400020.0192308
0.0542.88E-5SGTC_2654protoveratrine a 100.0 μMMicrosource (Natural product library)57022780.00961538
0.0533.99E-5SGTC_2131toluylene red 5.3 μMChembridge (Fragment library)111050.068181860S ribosome export
0.0534.11E-5SGTC_7014424-0219 185.0 μMChemDiv (Drug-like library)29117790.0126582calcium & mitochondrial duress
0.0525.73E-5SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.0151515
0.0526.99E-5SGTC_24215690543 90.7 μMChembridge (Fragment library)33979930.0555556
0.0491.62E-4SGTC_1989st071868 50.2 μMTimTec (Natural product derivative library)164140570.0434783
0.0491.84E-4SGTC_27947785382 27.3 μMChembridge (Drug-like library)22050810.0166667
0.0472.89E-4SGTC_2764methyldopa 94.7 μMMiscellaneous388530.0434783
0.0455.63E-4SGTC_1946st076057 65.1 μMTimTec (Natural product derivative library)9530090.0172414

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20725231554129 μM0.407407789545Chembridge (Fragment library)192.240920.43125
SGTC_21125488546200 μM0.375600757Chembridge (Fragment library)260.357942.13625TSC3-RPN4
SGTC_20795212404121 μM0.34375758462Chembridge (Fragment library)220.294081.34325
SGTC_20865274770176.62 μM0.333333689278Chembridge (Fragment library)254.31031.46325
SGTC_21105483698200 μM0.3243242848765Chembridge (Fragment library)258.342061.69125TSC3-RPN4
SGTC_20785212016200 μM0.3142861899708Chembridge (Fragment library)288.755362.1272560S ribosome export
SGTC_21135491891107.03 μM0.305556789979Chembridge (Fragment library)268.336881.91925TSC3-RPN4
SGTC_2071523095139.92 μM0.2972971799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4
SGTC_2074521200775.4 μM0.297297758611Chembridge (Fragment library)272.3007631.66826
SGTC_20775212015200 μM0.297297780900Chembridge (Fragment library)288.755362.12725