5324836

1-(4-morpholin-4-ylphenyl)pyrrole-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2134
Screen concentration 1.5 μM
Source Chembridge (Fragment library)
PubChem CID 699272
SMILES C1COCCN1C2=CC=C(C=C2)N3C(=O)C=CC3=O
Standardized SMILES O=C1C=CC(=O)N1c2ccc(cc2)N3CCOCC3
Molecular weight 258.2726
ALogP 1.24
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.7
% growth inhibition (Hom. pool) 3.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 699272
Download HIP data (tab-delimited text)  (excel)
Gene:ALG7(YBR243C)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:CCT6(YDR188W)|FD-Score:3.24|P-value:5.93E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:DSL1(YNL258C)|FD-Score:-4.71|P-value:1.23E-6|Clearance:0||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:FOL3(YMR113W)|FD-Score:-3.91|P-value:4.64E-5|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:NOP56(YLR197W)|FD-Score:5.36|P-value:4.26E-8|Clearance:0.77||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP85(YJR042W)|FD-Score:3.24|P-value:5.98E-4|Clearance:0.09||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:ORC3(YLL004W)|FD-Score:-4.49|P-value:3.59E-6|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PGA2(YNL149C)|FD-Score:-3.11|P-value:9.46E-4|Clearance:0||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:PSF3(YOL146W)|FD-Score:-3.18|P-value:7.44E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPP1(YHR062C)|FD-Score:4.59|P-value:2.21E-6|Clearance:0.55||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT5(YOR117W)|FD-Score:3.11|P-value:9.37E-4|Clearance:0.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSE1(YML049C)|FD-Score:-3.84|P-value:6.20E-5|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:TAF12(YDR145W)|FD-Score:4.04|P-value:2.63E-5|Clearance:0.3||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:UBC1(YDR177W)|FD-Score:3.15|P-value:8.20E-4|Clearance:0.04||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YPL044C(YPL044C_d)|FD-Score:3.74|P-value:9.12E-5|Clearance:0.5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:ALG7(YBR243C)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:CCT6(YDR188W)|FD-Score:3.24|P-value:5.93E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:DSL1(YNL258C)|FD-Score:-4.71|P-value:1.23E-6|Clearance:0||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:FOL3(YMR113W)|FD-Score:-3.91|P-value:4.64E-5|Clearance:0||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:NOP56(YLR197W)|FD-Score:5.36|P-value:4.26E-8|Clearance:0.77||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP85(YJR042W)|FD-Score:3.24|P-value:5.98E-4|Clearance:0.09||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:ORC3(YLL004W)|FD-Score:-4.49|P-value:3.59E-6|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PGA2(YNL149C)|FD-Score:-3.11|P-value:9.46E-4|Clearance:0||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:PSF3(YOL146W)|FD-Score:-3.18|P-value:7.44E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPP1(YHR062C)|FD-Score:4.59|P-value:2.21E-6|Clearance:0.55||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT5(YOR117W)|FD-Score:3.11|P-value:9.37E-4|Clearance:0.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSE1(YML049C)|FD-Score:-3.84|P-value:6.20E-5|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:TAF12(YDR145W)|FD-Score:4.04|P-value:2.63E-5|Clearance:0.3||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:UBC1(YDR177W)|FD-Score:3.15|P-value:8.20E-4|Clearance:0.04||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YPL044C(YPL044C_d)|FD-Score:3.74|P-value:9.12E-5|Clearance:0.5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 699272
Download HOP data (tab-delimited text)  (excel)
Gene:ARL3(YPL051W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG31(YDR022C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:ATP14(YLR295C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:AUS1(YOR011W)|FD-Score:-3.61|P-value:1.50E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BUD30(YDL151C_d)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAC2(YML102W)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CIN8(YEL061C)|FD-Score:4.78|P-value:8.70E-7||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CLA4(YNL298W)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG5(YNL051W)|FD-Score:-4.97|P-value:3.35E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSI2(YOL007C)|FD-Score:4.93|P-value:4.19E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene Gene:ECM33(YBR078W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:EMP47(YFL048C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FES1(YBR101C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FLO11(YIR019C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FMP16(YDR070C_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GFD2(YCL036W)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:ILM1(YJR118C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:JEN1(YKL217W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MAC1(YMR021C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MUD2(YKL074C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:OSW1(YOR255W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAU13(YHL046C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PEA2(YER149C)|FD-Score:-5.11|P-value:1.65E-7||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PHM6(YDR281C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PPM1(YDR435C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRS2(YER099C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:QCR2(YPR191W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:QDR1(YIL120W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD30(YDR419W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV Gene:RCE1(YMR274C)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RPA49(YNL248C)|FD-Score:-3.94|P-value:4.02E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPS23A(YGR118W)|FD-Score:4.43|P-value:4.78E-6||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RTS1(YOR014W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SEC72(YLR292C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGS1(YMR190C)|FD-Score:10.9|P-value:6.39E-28||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SKN7(YHR206W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNT309(YPR101W)|FD-Score:6.54|P-value:3.05E-11||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPC1(YJR010C-A)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SRL2(YLR082C)|FD-Score:-4.64|P-value:1.72E-6||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:STD1(YOR047C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:SUM1(YDR310C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SYT1(YPR095C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TFB6(YOR352W)|FD-Score:10.4|P-value:1.22E-25||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TGL2(YDR058C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TIF4631(YGR162W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGX2(YDL169C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:VAC14(YLR386W)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS28(YPL065W)|FD-Score:-4.29|P-value:8.77E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS74(YDR372C)|FD-Score:4.88|P-value:5.22E-7||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBR056W(YBR056W_p)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR072C-A(YBR072C-A_p)|FD-Score:-3.8|P-value:7.15E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR074W(YBR074W_p)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Putative metalloprotease Gene:YBR141C(YBR141C_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCL075W(YCL075W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR186C(YDR186C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER053C-A(YER053C-A_p)|FD-Score:-4.65|P-value:1.68E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YGL072C(YGL072C_d)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR050C(YGR050C_p)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YIL060W(YIL060W_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIR014W(YIR014W_p)|FD-Score:-3.73|P-value:9.69E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene Gene:YJL213W(YJL213W)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YKL118W(YKL118W_d)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL151C(YKL151C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR184W(YLR184W_d)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR257W(YLR257W_p)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YML100W-A(YML100W-A_p)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOL163W(YOL163W_p)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOR309C(YOR309C_d)|FD-Score:5.56|P-value:1.37E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPK3(YBR028C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:ARL3(YPL051W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG31(YDR022C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:ATP14(YLR295C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:AUS1(YOR011W)|FD-Score:-3.61|P-value:1.50E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BUD30(YDL151C_d)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAC2(YML102W)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CIN8(YEL061C)|FD-Score:4.78|P-value:8.70E-7||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CLA4(YNL298W)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG5(YNL051W)|FD-Score:-4.97|P-value:3.35E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSI2(YOL007C)|FD-Score:4.93|P-value:4.19E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene Gene:ECM33(YBR078W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:EMP47(YFL048C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FES1(YBR101C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FLO11(YIR019C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FMP16(YDR070C_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GFD2(YCL036W)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:ILM1(YJR118C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:JEN1(YKL217W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MAC1(YMR021C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MUD2(YKL074C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:OSW1(YOR255W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAU13(YHL046C)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PEA2(YER149C)|FD-Score:-5.11|P-value:1.65E-7||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PHM6(YDR281C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PPM1(YDR435C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRS2(YER099C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:QCR2(YPR191W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:QDR1(YIL120W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD30(YDR419W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV Gene:RCE1(YMR274C)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RPA49(YNL248C)|FD-Score:-3.94|P-value:4.02E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPS23A(YGR118W)|FD-Score:4.43|P-value:4.78E-6||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RTS1(YOR014W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SEC72(YLR292C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGS1(YMR190C)|FD-Score:10.9|P-value:6.39E-28||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SKN7(YHR206W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNT309(YPR101W)|FD-Score:6.54|P-value:3.05E-11||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPC1(YJR010C-A)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SRL2(YLR082C)|FD-Score:-4.64|P-value:1.72E-6||SGD DESC:Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation Gene:STD1(YOR047C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:SUM1(YDR310C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SYT1(YPR095C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TFB6(YOR352W)|FD-Score:10.4|P-value:1.22E-25||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TGL2(YDR058C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TIF4631(YGR162W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGX2(YDL169C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:VAC14(YLR386W)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS28(YPL065W)|FD-Score:-4.29|P-value:8.77E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS74(YDR372C)|FD-Score:4.88|P-value:5.22E-7||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBR056W(YBR056W_p)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR072C-A(YBR072C-A_p)|FD-Score:-3.8|P-value:7.15E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR074W(YBR074W_p)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Putative metalloprotease Gene:YBR141C(YBR141C_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCL075W(YCL075W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR186C(YDR186C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER053C-A(YER053C-A_p)|FD-Score:-4.65|P-value:1.68E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YGL072C(YGL072C_d)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR050C(YGR050C_p)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YIL060W(YIL060W_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIR014W(YIR014W_p)|FD-Score:-3.73|P-value:9.69E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene Gene:YJL213W(YJL213W)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YKL118W(YKL118W_d)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL151C(YKL151C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YLR184W(YLR184W_d)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR257W(YLR257W_p)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YML100W-A(YML100W-A_p)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOL163W(YOL163W_p)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOR309C(YOR309C_d)|FD-Score:5.56|P-value:1.37E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPK3(YBR028C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR197W5.364.26E-80.77NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YHR062C4.592.21E-60.55RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR145W4.042.63E-50.30TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YPL044C_d3.749.12E-50.50YPL044C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
YDR188W3.245.93E-40.00CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YJR042W3.245.98E-40.09NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YDR177W3.158.20E-40.04UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YOR117W3.119.37E-40.06RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YLR215C3.050.001150.06CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
YKL144C2.990.001420.15RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YMR131C2.830.002310.12RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YGL171W2.710.003390.04ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YDR212W2.670.003850.01TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YJL041W2.650.004020.29NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YBR152W2.360.009070.01SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR190C10.906.39E-28SGS1Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress
YOR352W10.401.22E-25TFB6Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YPR101W6.543.05E-11SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
YOR309C_d5.561.37E-8YOR309C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
YOL007C4.934.19E-7CSI2Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene
YDR372C4.885.22E-7VPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
YEL061C4.788.70E-7CIN8Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
YJR010C-A4.701.33E-6SPC1Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress
YDL151C_d4.651.63E-6BUD30_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YER107C4.592.22E-6GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YLR295C4.572.42E-6ATP14Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress
YGR118W4.434.78E-6RPS23ARibosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
YMR021C4.032.81E-5MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YGR050C_p4.022.95E-5YGR050C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YML102W4.013.10E-5CAC2Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription

GO enrichment analysis for SGTC_2134
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1193.97E-20SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0555556TSC3-RPN4
0.1093.76E-17SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.0645161TSC3-RPN4
0.1088.46E-17SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0784314
0.1032.46E-15SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.0877193
0.1001.25E-14SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.121212TSC3-RPN4
0.1001.26E-14SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0727273TSC3-RPN4
0.0984.95E-14SGTC_26133',4'-dimethoxydalbergione 24.6 μMMicrosource (Natural product library)1150190.0892857
0.0985.85E-14SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.084507TSC3-RPN4
0.0946.05E-13SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0895522
0.0938.09E-13SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0597015TSC3-RPN4
0.0899.51E-12SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.16129
0.0862.93E-11SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0545455TSC3-RPN4
0.0856.60E-11SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.195652TSC3-RPN4
0.0831.72E-10SGTC_24585808907 16.4 μMMiscellaneous27317470.189655
0.0832.15E-10SGTC_7543474-0146 171.0 μMChemDiv (Drug-like library)247620780.0617284copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2456577035974.46 μM0.3962263118787Miscellaneous412.45892.54717
SGTC_12220366-00741.18 μM0.365854286520ChemDiv (Drug-like library)245.230741.59204TSC3-RPN4
SGTC_235890371574 μM0.3333332388925Chembridge (Fragment library)231.250520.20513
SGTC_1689st02026553.49 μM0.3269235758320TimTec (Natural product derivative library)336.38443.40405
SGTC_2246729350791.56 μM0.318182887656Chembridge (Fragment library)244.245980.99413
SGTC_20104012422159 μM0.317073952393Chembridge (Fragment library)212.676021.59713
SGTC_23539059630146.67 μM0.3170734779114Chembridge (Fragment library)212.676021.59713
SGTC_8930958-036948.9 μM0.3148156819948ChemDiv (Drug-like library)391.259062.08915
SGTC_1824st05372368.6 μM0.276596675225TimTec (Natural product derivative library)291.347083.39704
SGTC_2155557042376 μM0.272727797402Chembridge (Fragment library)250.3118632.54403ergosterol biosynthesis