5328288

5-(thiophen-2-ylmethylidene)-1,3-diazinane-2,4,6-trione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2136
Screen concentration 199.3 μM
Source Chembridge (Fragment library)
PubChem CID 262395
SMILES C1=CSC(=C1)C=C2C(=O)NC(=O)NC2=O
Standardized SMILES O=C1NC(=O)C(=Cc2cccs2)C(=O)N1
Molecular weight 222.2205
ALogP 0.82
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.01
% growth inhibition (Hom. pool) 7.08


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 262395
Download HIP data (tab-delimited text)  (excel)
Gene:ALG7(YBR243C)|FD-Score:-4.17|P-value:1.52E-5|Clearance:0||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:CDC31(YOR257W)|FD-Score:3.97|P-value:3.58E-5|Clearance:0.22||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC53(YDL132W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.07||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CSE1(YGL238W)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.07||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:FAS2(YPL231W)|FD-Score:4.72|P-value:1.18E-6|Clearance:0.61||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GPI15(YNL038W)|FD-Score:-3.85|P-value:5.99E-5|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:IRA1(YBR140C)|FD-Score:-3.3|P-value:4.87E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LCB2(YDR062W)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.03||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MCM7(YBR202W)|FD-Score:5.36|P-value:4.14E-8|Clearance:0.53||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NMT1(YLR195C)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.22||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NOP7(YGR103W)|FD-Score:3.75|P-value:8.98E-5|Clearance:0.01||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:NUP82(YJL061W)|FD-Score:4.79|P-value:8.46E-7|Clearance:0.07||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:RHO1(YPR165W)|FD-Score:3.25|P-value:5.70E-4|Clearance:0.12||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB8(YOR224C)|FD-Score:-3.45|P-value:2.79E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:-3.91|P-value:4.68E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPL15A(YLR029C)|FD-Score:6.88|P-value:2.98E-12|Clearance:1.03||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:SEC13(YLR208W)|FD-Score:6.54|P-value:3.15E-11|Clearance:1.03||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SFT1(YKL006C-A)|FD-Score:-3.14|P-value:8.58E-4|Clearance:0||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SMX2(YFL017W-A)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SPC24(YMR117C)|FD-Score:5.51|P-value:1.83E-8|Clearance:0.15||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TAF8(YML114C)|FD-Score:4.83|P-value:6.89E-7|Clearance:0.04||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TAH18(YPR048W)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TIF35(YDR429C)|FD-Score:4.11|P-value:2.01E-5|Clearance:0.14||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM50(YPL063W)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.19||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:TOA2(YKL058W)|FD-Score:-3.27|P-value:5.29E-4|Clearance:0||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:UTP8(YGR128C)|FD-Score:3.25|P-value:5.67E-4|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:ALG7(YBR243C)|FD-Score:-4.17|P-value:1.52E-5|Clearance:0||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:CDC31(YOR257W)|FD-Score:3.97|P-value:3.58E-5|Clearance:0.22||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC53(YDL132W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.07||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CSE1(YGL238W)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.07||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:FAS2(YPL231W)|FD-Score:4.72|P-value:1.18E-6|Clearance:0.61||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GPI15(YNL038W)|FD-Score:-3.85|P-value:5.99E-5|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:IRA1(YBR140C)|FD-Score:-3.3|P-value:4.87E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LCB2(YDR062W)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.03||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MCM7(YBR202W)|FD-Score:5.36|P-value:4.14E-8|Clearance:0.53||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NMT1(YLR195C)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.22||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:NOP7(YGR103W)|FD-Score:3.75|P-value:8.98E-5|Clearance:0.01||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:NUP82(YJL061W)|FD-Score:4.79|P-value:8.46E-7|Clearance:0.07||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:RHO1(YPR165W)|FD-Score:3.25|P-value:5.70E-4|Clearance:0.12||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB8(YOR224C)|FD-Score:-3.45|P-value:2.79E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:-3.91|P-value:4.68E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPL15A(YLR029C)|FD-Score:6.88|P-value:2.98E-12|Clearance:1.03||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:SEC13(YLR208W)|FD-Score:6.54|P-value:3.15E-11|Clearance:1.03||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SFT1(YKL006C-A)|FD-Score:-3.14|P-value:8.58E-4|Clearance:0||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SMX2(YFL017W-A)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SPC24(YMR117C)|FD-Score:5.51|P-value:1.83E-8|Clearance:0.15||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TAF8(YML114C)|FD-Score:4.83|P-value:6.89E-7|Clearance:0.04||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TAH18(YPR048W)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TIF35(YDR429C)|FD-Score:4.11|P-value:2.01E-5|Clearance:0.14||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM50(YPL063W)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.19||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:TOA2(YKL058W)|FD-Score:-3.27|P-value:5.29E-4|Clearance:0||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:UTP8(YGR128C)|FD-Score:3.25|P-value:5.67E-4|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 262395
Download HOP data (tab-delimited text)  (excel)
Gene:AAD6(YFL056C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ACE2(YLR131C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ACO2(YJL200C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:ARL1(YBR164C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG21(YPL100W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:ATP11(YNL315C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP5(YDR298C)|FD-Score:-7.67|P-value:8.69E-15||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BFR1(YOR198C)|FD-Score:-3.76|P-value:8.39E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:CDC26(YFR036W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIN8(YEL061C)|FD-Score:-4.8|P-value:7.98E-7||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CRH1(YGR189C)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CTF4(YPR135W)|FD-Score:4.55|P-value:2.71E-6||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DYN3(YMR299C)|FD-Score:4.38|P-value:5.87E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EMC5(YIL027C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FRE5(YOR384W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN3(YKR026C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCY1(YOR120W)|FD-Score:4.98|P-value:3.17E-7||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GIS2(YNL255C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GLY1(YEL046C)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GTT2(YLL060C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HOS4(YIL112W)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:IAH1(YOR126C)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IMP2'(YIL154C)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISR1(YPR106W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:JNM1(YMR294W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LDB19(YOR322C)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LHS1(YKL073W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MEF1(YLR069C)|FD-Score:-5.51|P-value:1.84E-8||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MON2(YNL297C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRPL10(YNL284C)|FD-Score:-3.94|P-value:4.11E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPL28(YDR462W)|FD-Score:-6.29|P-value:1.55E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS35(YGR165W)|FD-Score:-4.87|P-value:5.48E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSB2(YGR014W)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSW1(YDR268W)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NAM2(YLR382C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NGL1(YOL042W)|FD-Score:-3.73|P-value:9.55E-5||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OPI9(YLR338W_d)|FD-Score:6.27|P-value:1.75E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OST3(YOR085W)|FD-Score:-4.48|P-value:3.71E-6||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PRM5(YIL117C)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PTR2(YKR093W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAD16(YBR114W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD59(YDL059C)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RIM21(YNL294C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL29(YFR032C-A)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL31B(YLR406C)|FD-Score:12.4|P-value:1.84E-35||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:-6.02|P-value:8.47E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RVS161(YCR009C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SDS3(YIL084C)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SPL2(YHR136C)|FD-Score:6.43|P-value:6.40E-11||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SRB5(YGR104C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SUR4(YLR372W)|FD-Score:-4.56|P-value:2.51E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYS1(YJL004C)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THP1(YOL072W)|FD-Score:5.22|P-value:8.72E-8||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:VID30(YGL227W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VPS28(YPL065W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:YAL065C(YAL065C_p)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YAR028W(YAR028W_p)|FD-Score:4|P-value:3.10E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YCL075W(YCL075W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR102C(YCR102C_p)|FD-Score:5.36|P-value:4.08E-8||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR274C(YDR274C_d)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL023C(YEL023C_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YFL034W(YFL034W_p)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YHR112C(YHR112C)|FD-Score:-4.77|P-value:8.99E-7||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YHR177W(YHR177W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIP5(YGL161C)|FD-Score:-5.77|P-value:4.07E-9||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL107C(YJL107C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi Gene:YJL182C(YJL182C_d)|FD-Score:-4.83|P-value:6.85E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YKL115C(YKL115C_d)|FD-Score:5.34|P-value:4.78E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLR171W(YLR171W_d)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML009W-B(YML009W-B_d)|FD-Score:-3.88|P-value:5.25E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNL122C(YNL122C_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YOR072W(YOR072W_d)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YSY6(YBR162W-A)|FD-Score:5.34|P-value:4.75E-8||SGD DESC:Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion Gene:AAD6(YFL056C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ACE2(YLR131C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ACO2(YJL200C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:ARL1(YBR164C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG21(YPL100W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:ATP11(YNL315C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP5(YDR298C)|FD-Score:-7.67|P-value:8.69E-15||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BFR1(YOR198C)|FD-Score:-3.76|P-value:8.39E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:CDC26(YFR036W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIN8(YEL061C)|FD-Score:-4.8|P-value:7.98E-7||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CRH1(YGR189C)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CTF4(YPR135W)|FD-Score:4.55|P-value:2.71E-6||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DYN3(YMR299C)|FD-Score:4.38|P-value:5.87E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EMC5(YIL027C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FRE5(YOR384W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN3(YKR026C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCY1(YOR120W)|FD-Score:4.98|P-value:3.17E-7||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GIS2(YNL255C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GLY1(YEL046C)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GTT2(YLL060C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HOS4(YIL112W)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:IAH1(YOR126C)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IMP2'(YIL154C)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISR1(YPR106W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:JNM1(YMR294W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LDB19(YOR322C)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LHS1(YKL073W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MEF1(YLR069C)|FD-Score:-5.51|P-value:1.84E-8||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MON2(YNL297C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRPL10(YNL284C)|FD-Score:-3.94|P-value:4.11E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPL28(YDR462W)|FD-Score:-6.29|P-value:1.55E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS35(YGR165W)|FD-Score:-4.87|P-value:5.48E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MSB2(YGR014W)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSW1(YDR268W)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NAM2(YLR382C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NGL1(YOL042W)|FD-Score:-3.73|P-value:9.55E-5||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OPI9(YLR338W_d)|FD-Score:6.27|P-value:1.75E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OST3(YOR085W)|FD-Score:-4.48|P-value:3.71E-6||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PRM5(YIL117C)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PTR2(YKR093W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAD16(YBR114W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD59(YDL059C)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RIM21(YNL294C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL29(YFR032C-A)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL31B(YLR406C)|FD-Score:12.4|P-value:1.84E-35||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:-6.02|P-value:8.47E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RVS161(YCR009C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SDS3(YIL084C)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SPL2(YHR136C)|FD-Score:6.43|P-value:6.40E-11||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SRB5(YGR104C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SUR4(YLR372W)|FD-Score:-4.56|P-value:2.51E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYS1(YJL004C)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THP1(YOL072W)|FD-Score:5.22|P-value:8.72E-8||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:VID30(YGL227W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VPS28(YPL065W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:YAL065C(YAL065C_p)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YAR028W(YAR028W_p)|FD-Score:4|P-value:3.10E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YCL075W(YCL075W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR102C(YCR102C_p)|FD-Score:5.36|P-value:4.08E-8||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR274C(YDR274C_d)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL023C(YEL023C_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YFL034W(YFL034W_p)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YHR112C(YHR112C)|FD-Score:-4.77|P-value:8.99E-7||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YHR177W(YHR177W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YIP5(YGL161C)|FD-Score:-5.77|P-value:4.07E-9||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL107C(YJL107C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi Gene:YJL182C(YJL182C_d)|FD-Score:-4.83|P-value:6.85E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YKL115C(YKL115C_d)|FD-Score:5.34|P-value:4.78E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLR171W(YLR171W_d)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML009W-B(YML009W-B_d)|FD-Score:-3.88|P-value:5.25E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNL122C(YNL122C_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YOR072W(YOR072W_d)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YSY6(YBR162W-A)|FD-Score:5.34|P-value:4.75E-8||SGD DESC:Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR029C6.882.98E-121.03RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YLR208W6.543.15E-111.03SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YMR117C5.511.83E-80.15SPC24Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YBR202W5.364.14E-80.53MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YML114C4.836.89E-70.04TAF8TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation
YJL061W4.798.46E-70.07NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YPL231W4.721.18E-60.61FAS2Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
YDR429C4.112.01E-50.14TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YOR257W3.973.58E-50.23CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YGR103W3.758.98E-50.01NOP7Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
YPL063W3.749.28E-50.20TIM50Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
YDL132W3.541.98E-40.07CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YLR195C3.472.56E-40.22NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YGR128C3.255.67E-40.00UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YPR165W3.255.70E-40.12RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR406C12.401.84E-35RPL31BRibosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
YHR136C6.436.40E-11SPL2Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm
YLR338W_d6.271.75E-10OPI9_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C
YCR102C_p5.364.08E-8YCR102C_pPutative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family
YBR162W-A5.344.75E-8YSY6Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
YKL115C_d5.344.78E-8YKL115C_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
YOL072W5.228.72E-8THP1Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
YOR120W4.983.17E-7GCY1Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
YLR131C4.741.06E-6ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YIL154C4.671.48E-6IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YFR036W4.671.51E-6CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YPR135W4.552.71E-6CTF4Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
YOR322C4.532.95E-6LDB19Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye
YMR299C4.385.87E-6DYN3Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
YNL294C4.241.10E-5RIM21pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH

GO enrichment analysis for SGTC_2136
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1187.76E-20SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.0892857
0.1141.35E-18SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.111111
0.1018.35E-15SGTC_1666st014829 29.0 μMTimTec (Natural product derivative library)38582300.0533333calcium & mitochondrial duress
0.1001.37E-14SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.0862069
0.0992.79E-14SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0806452
0.0943.98E-13SGTC_890302-0250 142.1 μMChemDiv (Drug-like library)42035180.0566038
0.0863.48E-11SGTC_1104tacrolimus 29.8 μMNIH Clinical Collection235817960.0442478
0.0863.57E-11SGTC_7014424-0219 185.0 μMChemDiv (Drug-like library)29117790.0595238calcium & mitochondrial duress
0.0741.01E-8SGTC_14163966-0321 25.3 μMChemDiv (Drug-like library)5825300.170213
0.0741.52E-8SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.057971calcium & mitochondrial duress
0.0714.63E-8SGTC_9751335-0046 28.5 μMChemDiv (Drug-like library)164867400.0588235RPP1 & pyrimidine depletion
0.0706.31E-8SGTC_400oxethazaine 37.5 μMMiscellaneous46210.0862069calcium & mitochondrial duress
0.0706.69E-8SGTC_1603453-0671 289.7 μMChemDiv (Drug-like library)53222680.0779221
0.0708.46E-8SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.1
0.0681.41E-7SGTC_9943910-0338 138.0 μMChemDiv (Drug-like library)34794810.0547945calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2065522159634.26 μM0.75788581Chembridge (Fragment library)238.286141.71724
SGTC_22095-(5-methyl-2-thenylidene)-Barbituric acid122.83 μM0.527778616764Chembridge (Fragment library)236.247120.96324
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.5128211241132ChemDiv (Drug-like library)227.32642.79414
SGTC_2032518511225.99 μM0.3777785718189Chembridge (Fragment library)241.286781.54415
SGTC_2448518511227.17 μM0.3777785718189Chembridge (Fragment library)241.286781.54415
SGTC_6033794-002345.9 μM0.3333333802260ChemDiv (Drug-like library)356.759823.09624
SGTC_21465545642200 μM0.311111825326Chembridge (Fragment library)202.232441.21713
SGTC_23067482397179.52 μM0.304348891448Chembridge (Fragment library)282.702882.18634RPP1 & pyrimidine depletion
SGTC_2227731222164.1 μM0.297872887188Chembridge (Fragment library)231.273661.77814
SGTC_23276189656200 μM0.2765965349088Chembridge (Fragment library)282.702882.18634
SGTC_2165572719732.14 μM0.2666671361712Chembridge (Fragment library)211.26081.60414