5575593

3,4-dimethyl-N-(pyridin-4-ylmethyl)aniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2138
Screen concentration 77.7 μM
Source Chembridge (Fragment library)
PubChem CID 762213
SMILES CC1=C(C=C(C=C1)NCC2=CC=NC=C2)C
Standardized SMILES Cc1ccc(NCc2ccncc2)cc1C
Molecular weight 212.2902
ALogP 3.04
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.22
% growth inhibition (Hom. pool) 3.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 762213
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:3.25|P-value:5.75E-4|Clearance:0.37||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:CCT4(YDL143W)|FD-Score:-3.65|P-value:1.30E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:FCP1(YMR277W)|FD-Score:-4.04|P-value:2.73E-5|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:MAS1(YLR163C)|FD-Score:-3.82|P-value:6.69E-5|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MRS6(YOR370C)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.13||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:NSP1(YJL041W)|FD-Score:-3.25|P-value:5.74E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:SEC12(YNR026C)|FD-Score:-3.63|P-value:1.43E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SSY5(YJL156C)|FD-Score:4.77|P-value:9.20E-7|Clearance:1.03||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TIF35(YDR429C)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.08||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YCS4(YLR272C)|FD-Score:4.48|P-value:3.69E-6|Clearance:1.03||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YNL150W(YNL150W_d)|FD-Score:-3.95|P-value:3.87E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:ARB1(YER036C)|FD-Score:3.25|P-value:5.75E-4|Clearance:0.37||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:CCT4(YDL143W)|FD-Score:-3.65|P-value:1.30E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:FCP1(YMR277W)|FD-Score:-4.04|P-value:2.73E-5|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:MAS1(YLR163C)|FD-Score:-3.82|P-value:6.69E-5|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MRS6(YOR370C)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.13||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:NSP1(YJL041W)|FD-Score:-3.25|P-value:5.74E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:SEC12(YNR026C)|FD-Score:-3.63|P-value:1.43E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SSY5(YJL156C)|FD-Score:4.77|P-value:9.20E-7|Clearance:1.03||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TIF35(YDR429C)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.08||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YCS4(YLR272C)|FD-Score:4.48|P-value:3.69E-6|Clearance:1.03||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YNL150W(YNL150W_d)|FD-Score:-3.95|P-value:3.87E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 762213
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AHT1(YHR093W_d)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:APE2(YKL157W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC50(YCR094W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS3(YBR023C)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CRN1(YLR429W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CTH1(YDR151C)|FD-Score:-3.78|P-value:7.91E-5||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:CTK3(YML112W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DDI3(YNL335W_p)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:ECM23(YPL021W)|FD-Score:4.89|P-value:5.10E-7||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:ECM8(YBR076W)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Non-essential protein of unknown function Gene:ERG28(YER044C)|FD-Score:-4.03|P-value:2.81E-5||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:GTT1(YIR038C)|FD-Score:4.98|P-value:3.19E-7||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:HTL1(YCR020W-B)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:LAG2(YOL025W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MAC1(YMR021C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAK3(YPR051W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MAL11(YGR289C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MVB12(YGR206W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:MYO4(YAL029C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:PDR10(YOR328W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PET54(YGR222W)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PRO2(YOR323C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RPL35B(YDL136W)|FD-Score:-3.98|P-value:3.50E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:SDC25(YLL016W)|FD-Score:-4.25|P-value:1.09E-5||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:3.96|P-value:3.67E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNT309(YPR101W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SSD1(YDR293C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TGL2(YDR058C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TRP4(YDR354W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP2(YOR124C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VPS25(YJR102C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS8(YAL002W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YDL032W(YDL032W_d)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDR467C(YDR467C_d)|FD-Score:-3.78|P-value:7.90E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YGR164W(YGR164W_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHL042W(YHL042W_p)|FD-Score:-3.9|P-value:4.83E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YKL107W(YKL107W_p)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YLR125W(YLR125W_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YOL162W(YOL162W_p)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YPK1(YKL126W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL199C(YPL199C_p)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPT6(YLR262C)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YTA7(YGR270W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ACM1(YPL267W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AHT1(YHR093W_d)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:APE2(YKL157W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC50(YCR094W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS3(YBR023C)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CRN1(YLR429W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CTH1(YDR151C)|FD-Score:-3.78|P-value:7.91E-5||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:CTK3(YML112W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DDI3(YNL335W_p)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:ECM23(YPL021W)|FD-Score:4.89|P-value:5.10E-7||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:ECM8(YBR076W)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Non-essential protein of unknown function Gene:ERG28(YER044C)|FD-Score:-4.03|P-value:2.81E-5||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:GTT1(YIR038C)|FD-Score:4.98|P-value:3.19E-7||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:HTL1(YCR020W-B)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:LAG2(YOL025W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MAC1(YMR021C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAK3(YPR051W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MAL11(YGR289C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MVB12(YGR206W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:MYO4(YAL029C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:PDR10(YOR328W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PET54(YGR222W)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PRO2(YOR323C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RPL35B(YDL136W)|FD-Score:-3.98|P-value:3.50E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:SDC25(YLL016W)|FD-Score:-4.25|P-value:1.09E-5||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:3.96|P-value:3.67E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNT309(YPR101W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SSD1(YDR293C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TGL2(YDR058C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TRP4(YDR354W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP2(YOR124C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VPS25(YJR102C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS8(YAL002W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YDL032W(YDL032W_d)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDR467C(YDR467C_d)|FD-Score:-3.78|P-value:7.90E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YGR164W(YGR164W_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHL042W(YHL042W_p)|FD-Score:-3.9|P-value:4.83E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YKL107W(YKL107W_p)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YLR125W(YLR125W_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YOL162W(YOL162W_p)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YPK1(YKL126W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL199C(YPL199C_p)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPT6(YLR262C)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YTA7(YGR270W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL156C4.779.20E-71.03SSY5Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p
YLR272C4.483.69E-61.03YCS4Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YDR429C3.462.73E-40.08TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YOR370C3.383.66E-40.13MRS6Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
YER036C3.255.75E-40.37ARB1ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
YOR176W2.880.002010.08HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YER009W2.800.002550.07NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YLL003W2.740.003120.03SFI1Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C
YOL077C2.710.003410.05BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YLR229C2.660.003910.01CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YPR177C_d2.650.003990.09YPR177C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
YFL017C2.560.005220.12GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YKL014C2.440.007300.02URB1Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit
YLR167W2.420.007660.03RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YNL118C2.390.008420.09DCP2Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR038C4.983.19E-7GTT1ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p
YPL021W4.895.10E-7ECM23Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
YLR262C4.661.56E-6YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YJR102C4.602.13E-6VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YKL126W4.221.21E-5YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YPR101W4.221.25E-5SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
YPR051W4.201.33E-5MAK3Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus
YAL002W4.171.54E-5VPS8Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
YLR372W4.003.17E-5SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YLR139C3.963.67E-5SLS1Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
YOR323C3.885.21E-5PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YCR020W-B3.875.55E-5HTL1Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YGR222W3.846.11E-5PET54Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing
YPL199C_p3.787.81E-5YPL199C_pPutative protein of unknown function, predicted to be palmitoylated
YML112W3.701.08E-4CTK3Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2138
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1382.03E-26SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0769231
0.1291.78E-23SGTC_31059121040 49.5 μMChembridge (Drug-like library)414489070.196429
0.1219.47E-21SGTC_21455634607 200.0 μMChembridge (Fragment library)3086620.176471
0.1056.07E-16SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.0806452
0.1024.11E-15SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.12963
0.1023.74E-15SGTC_14103909-8106 77.9 μMChemDiv (Drug-like library)10392100.161765
0.1001.38E-14SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0945946
0.0943.45E-13SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.142857
0.0944.01E-13SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.130435
0.0937.35E-13SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.103448
0.0921.38E-12SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.0595238calcium & mitochondrial duress
0.0881.50E-11SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.0833333
0.0856.37E-11SGTC_12021988-0068 29.6 μMChemDiv (Drug-like library)28504800.0985916RNA processing & uracil transport
0.0831.47E-10SGTC_2620irigenin 100.0 μMMicrosource (Natural product library)54641700.0625
0.0813.65E-10SGTC_813k007-0158 173.0 μMChemDiv (Drug-like library)65239160.211538fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21195528445120.64 μM0.666667791935Chembridge (Fragment library)212.290243.04412
SGTC_21485546263113.57 μM0.512821792097Chembridge (Fragment library)216.2541232.76313
SGTC_20975376186200 μM0.425742755Chembridge (Fragment library)198.263662.55712
SGTC_11770983-020423.6 μM0.382979766818ChemDiv (Drug-like library)318.369123.99613
SGTC_21005377183190 μM0.3695652844202Chembridge (Fragment library)250.316762.6631460S ribosome export
SGTC_11453546-059710.2 μM0.36875334ChemDiv (Drug-like library)293.406024.39812mitochondrial processes
SGTC_10553448-067286 μM0.347826748378ChemDiv (Drug-like library)302.369723.92412
SGTC_22917917025104.35 μM0.3269232964612Chembridge (Fragment library)280.321143.03813iron homeostasis
SGTC_21535569715200 μM0.3260872054701Chembridge (Fragment library)281.13732.79912iron homeostasis
SGTC_23649055074200 μM0.326470630Chembridge (Fragment library)275.733462.52922