5586273

1-(3-chloro-4-methylphenyl)pyrrolidine-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2140
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 854421
SMILES CC1=C(C=C(C=C1)N2C(=O)CCC2=O)Cl
Standardized SMILES Cc1ccc(cc1Cl)N2C(=O)CCC2=O
Molecular weight 223.6556
ALogP 2.14
H-bond donor count 0
H-bond acceptor count 2
Response signature RSC complex & mRNA processing

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.49
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 854421
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:5.45|P-value:2.46E-8|Clearance:0.21||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:4.38|P-value:5.91E-6|Clearance:0.5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:FCP1(YMR277W)|FD-Score:4.46|P-value:4.12E-6|Clearance:0.08||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LSM2(YBL026W)|FD-Score:6.71|P-value:9.63E-12|Clearance:0.64||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:5.96|P-value:1.25E-9|Clearance:0.51||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MMS21(YEL019C)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NUP49(YGL172W)|FD-Score:7.13|P-value:5.14E-13|Clearance:0.64||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP57(YGR119C)|FD-Score:5.99|P-value:1.02E-9|Clearance:0.03||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PAB1(YER165W)|FD-Score:3.88|P-value:5.21E-5|Clearance:0.01||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PWP2(YCR057C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.15||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RHO1(YPR165W)|FD-Score:6.87|P-value:3.18E-12|Clearance:0.64||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL32(YBL092W)|FD-Score:4.77|P-value:9.12E-7|Clearance:0.31||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPO26(YPR187W)|FD-Score:3.1|P-value:9.83E-4|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRP15(YPR143W)|FD-Score:3.76|P-value:8.57E-5|Clearance:0.44||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RSC58(YLR033W)|FD-Score:3.12|P-value:9.06E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.87|P-value:5.35E-5|Clearance:0.12||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.24|P-value:8.02E-8|Clearance:0.47||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SPC105(YGL093W)|FD-Score:6.63|P-value:1.64E-11|Clearance:0.64||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:YDL152W(YDL152W_d)|FD-Score:3.32|P-value:4.49E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YTH1(YPR107C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:5.45|P-value:2.46E-8|Clearance:0.21||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:4.38|P-value:5.91E-6|Clearance:0.5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:FCP1(YMR277W)|FD-Score:4.46|P-value:4.12E-6|Clearance:0.08||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LSM2(YBL026W)|FD-Score:6.71|P-value:9.63E-12|Clearance:0.64||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:5.96|P-value:1.25E-9|Clearance:0.51||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MMS21(YEL019C)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NUP49(YGL172W)|FD-Score:7.13|P-value:5.14E-13|Clearance:0.64||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP57(YGR119C)|FD-Score:5.99|P-value:1.02E-9|Clearance:0.03||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PAB1(YER165W)|FD-Score:3.88|P-value:5.21E-5|Clearance:0.01||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PWP2(YCR057C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.15||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RHO1(YPR165W)|FD-Score:6.87|P-value:3.18E-12|Clearance:0.64||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL32(YBL092W)|FD-Score:4.77|P-value:9.12E-7|Clearance:0.31||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPO26(YPR187W)|FD-Score:3.1|P-value:9.83E-4|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRP15(YPR143W)|FD-Score:3.76|P-value:8.57E-5|Clearance:0.44||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RSC58(YLR033W)|FD-Score:3.12|P-value:9.06E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.87|P-value:5.35E-5|Clearance:0.12||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.24|P-value:8.02E-8|Clearance:0.47||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SPC105(YGL093W)|FD-Score:6.63|P-value:1.64E-11|Clearance:0.64||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:YDL152W(YDL152W_d)|FD-Score:3.32|P-value:4.49E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YTH1(YPR107C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 854421
Download HOP data (tab-delimited text)  (excel)
Gene:AAC3(YBR085W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:AHT1(YHR093W_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ARL3(YPL051W)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP6(YLR085C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:CHD1(YER164W)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CPR8(YNR028W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:FYV1(YDR024W_d)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GAC1(YOR178C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:HEL1(YKR017C_p)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HSP78(YDR258C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:JID1(YPR061C)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:MSM1(YGR171C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:NPR2(YEL062W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NQM1(YGR043C)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:PDC1(YLR044C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PEP12(YOR036W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:QCR7(YDR529C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:REX2(YLR059C)|FD-Score:3.92|P-value:4.43E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RPA49(YNL248C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPS17A(YML024W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:SAT4(YCR008W)|FD-Score:4.52|P-value:3.08E-6||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SOH1(YGL127C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPO7(YAL009W)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:THI21(YPL258C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TMA17(YDL110C)|FD-Score:-4.32|P-value:7.93E-6||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:TVP38(YKR088C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VPS71(YML041C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR013C(YBR013C_p)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR027C(YBR027C_d)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR090C(YBR090C_p)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDL050C(YDL050C_d)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL082W(YGL082W_p)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGR064W(YGR064W_d)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Putative protein of unknown function Gene:YKL169C(YKL169C_d)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YMR130W(YMR130W_p)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YMR153C-A(YMR153C-A_d)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YPL108W(YPL108W_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPL257W(YPL257W_p)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPT53(YNL093W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:AAC3(YBR085W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication Gene:AHT1(YHR093W_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ARL3(YPL051W)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP6(YLR085C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:CHD1(YER164W)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CPR8(YNR028W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:FYV1(YDR024W_d)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GAC1(YOR178C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:HEL1(YKR017C_p)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HSP78(YDR258C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:JID1(YPR061C)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:MSM1(YGR171C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:NPR2(YEL062W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NQM1(YGR043C)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:PDC1(YLR044C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PEP12(YOR036W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:QCR7(YDR529C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:REX2(YLR059C)|FD-Score:3.92|P-value:4.43E-5||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RPA49(YNL248C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPS17A(YML024W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:SAT4(YCR008W)|FD-Score:4.52|P-value:3.08E-6||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SOH1(YGL127C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPO7(YAL009W)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:THI21(YPL258C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TMA17(YDL110C)|FD-Score:-4.32|P-value:7.93E-6||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:TVP38(YKR088C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VPS71(YML041C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR013C(YBR013C_p)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR027C(YBR027C_d)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR090C(YBR090C_p)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YDL050C(YDL050C_d)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL082W(YGL082W_p)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGR064W(YGR064W_d)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Putative protein of unknown function Gene:YKL169C(YKL169C_d)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YMR130W(YMR130W_p)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YMR153C-A(YMR153C-A_d)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YPL108W(YPL108W_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPL257W(YPL257W_p)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPT53(YNL093W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL172W7.135.14E-130.64NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YPR165W6.873.18E-120.64RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YBL026W6.719.63E-120.64LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YGL093W6.631.64E-110.64SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YGR119C5.991.02E-90.03NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YPL169C5.961.25E-90.51MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YNR016C5.452.46E-80.21ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YML127W5.248.02E-80.47RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YBL092W4.779.12E-70.31RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YMR277W4.464.12E-60.08FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YJL081C4.385.91E-60.50ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YER165W3.885.21E-50.01PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YFR037C3.875.35E-50.12RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YPR143W3.768.57E-50.44RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YDL152W_d3.324.49E-40.05YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL051W5.161.22E-7ARL3GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
YAL009W5.101.71E-7SPO7Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
YCR008W4.523.08E-6SAT4Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
YBR013C_p4.052.61E-5YBR013C_pPutative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
YLR085C3.953.92E-5ARP6Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YLR059C3.924.43E-5REX23'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease
YPR061C3.885.26E-5JID1Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
YGR064W_d3.711.04E-4YGR064W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C
YNL093W3.701.06E-4YPT53Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
YBR027C_d3.631.43E-4YBR027C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL108W_p3.581.73E-4YPL108W_pCytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YPL258C3.551.94E-4THI21Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p
YKR088C3.551.95E-4TVP38Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YNL248C3.492.43E-4RPA49RNA polymerase I subunit A49
YKL169C_d3.393.46E-4YKL169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38

GO enrichment analysis for SGTC_2140
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3586.24E-178SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.114754RSC complex & mRNA processing
0.3549.56E-174SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.116667RSC complex & mRNA processing
0.3041.56E-126SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.261905RSC complex & mRNA processing
0.2855.90E-111SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.1RSC complex & mRNA processing
0.2821.24E-108SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.104167RSC complex & mRNA processing
0.2553.00E-88SGTC_23619034121 116.0 μMChembridge (Fragment library)64969170.113208RSC complex & mRNA processing
0.2519.45E-86SGTC_6731082-0474 110.0 μMChemDiv (Drug-like library)30980460.127273RSC complex & mRNA processing
0.2471.37E-82SGTC_14934348-0038 111.0 μMChemDiv (Drug-like library)8004240.105263RSC complex & mRNA processing
0.2471.69E-82SGTC_8710574-0004 149.0 μMChemDiv (Drug-like library)95511730.0983607
0.2421.25E-79SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.1RSC complex & mRNA processing
0.2329.52E-73SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0943396RSC complex & mRNA processing
0.2275.73E-70SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.0833333cell wall
0.2265.47E-69SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.0816327RSC complex & mRNA processing
0.2198.59E-65SGTC_28909046729 58.4 μMChembridge (Drug-like library)9033290.163636Golgi
0.2183.22E-64SGTC_12290139-0193 12.2 μMChemDiv (Drug-like library)30907520.0666667RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14904089-0339670 nM0.348837742542ChemDiv (Drug-like library)274.166264.19402
SGTC_215856571233.62 μM0.3488372858827Chembridge (Fragment library)269.29521.81603
SGTC_3177910750649.47 μM0.3148158897194Chembridge (Drug-like library)314.809263.76512ERG2
SGTC_3178910750649.47 μM0.3148158897194Chembridge (Drug-like library)314.809263.76512
SGTC_2246729350791.56 μM0.311111887656Chembridge (Fragment library)244.245980.99413
SGTC_296090793895.97 μM0.29310316457355Chembridge (Drug-like library)310.775962.36514
SGTC_20685226020147 μM0.2926831810509Chembridge (Fragment library)195.602441.82112
SGTC_20875276107200 μM0.288889144405Chembridge (Fragment library)238.670241.51713
SGTC_2783773816571.43 μM0.2745117201110Chembridge (Drug-like library)289.756743.55612
SGTC_2809796413312.99 μM0.2666671253665Chembridge (Drug-like library)420.332224.4751360S ribosome export
SGTC_3284910817164.86 μM0.2666678687505Chembridge (Drug-like library)373.876463.7214