5623677

(5Z)-5-(5-fluoro-2-oxo-1H-indol-3-ylidene)-2-sulfanylidene-1,3-thiazolidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2143
Screen concentration 140.5 μM
Source Chembridge (Fragment library)
PubChem CID 5375313
SMILES C1=CC2=C(C=C1F)C(=C3C(=O)NC(=S)S3)C(=O)N2
Standardized SMILES Fc1ccc2NC(=O)C(=C3SC(=S)NC3=O)c2c1
Molecular weight 280.298
ALogP 1.96
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.39
% growth inhibition (Hom. pool) -1.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5375313
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:DRE2(YKR071C)|FD-Score:-3.91|P-value:4.67E-5|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FIP1(YJR093C)|FD-Score:-3.55|P-value:1.91E-4|Clearance:0||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:PDC2(YDR081C)|FD-Score:-3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRI2(YKL045W)|FD-Score:-3.81|P-value:6.98E-5|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PZF1(YPR186C)|FD-Score:4.31|P-value:8.09E-6|Clearance:0.63||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RET2(YFR051C)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.52||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPT4(YOR259C)|FD-Score:-3.87|P-value:5.48E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SPC3(YLR066W)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.63||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:UTP5(YDR398W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.07||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YNL150W(YNL150W_d)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPD1(YDL235C)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:BMS1(YPL217C)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:DRE2(YKR071C)|FD-Score:-3.91|P-value:4.67E-5|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FIP1(YJR093C)|FD-Score:-3.55|P-value:1.91E-4|Clearance:0||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:PDC2(YDR081C)|FD-Score:-3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRI2(YKL045W)|FD-Score:-3.81|P-value:6.98E-5|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PZF1(YPR186C)|FD-Score:4.31|P-value:8.09E-6|Clearance:0.63||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RET2(YFR051C)|FD-Score:3.49|P-value:2.44E-4|Clearance:0.52||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPT4(YOR259C)|FD-Score:-3.87|P-value:5.48E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SPC3(YLR066W)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.63||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:UTP5(YDR398W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.07||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YNL150W(YNL150W_d)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPD1(YDL235C)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5375313
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ACO2(YJL200C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:AFT1(YGL071W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:CAF120(YNL278W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CDC10(YCR002C)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:ELP2(YGR200C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:FLD1(YLR404W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:GIS1(YDR096W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:HUB1(YNR032C-A)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:MRPL37(YBR268W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH3(YCR092C)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:PAM17(YKR065C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PCI8(YIL071C)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PDR10(YOR328W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PEX12(YMR026C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:RDS1(YCR106W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RIM4(YHL024W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:SER1(YOR184W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SIP4(YJL089W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SIZ1(YDR409W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLS1(YLR139C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMA1(YPL027W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SRL1(YOR247W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SUB1(YMR039C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:TAL1(YLR354C)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:UBP15(YMR304W)|FD-Score:-4.07|P-value:2.37E-5||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:YEA6(YEL006W)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YLL020C(YLL020C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR232W(YLR232W_d)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Gene:YMR253C(YMR253C_p)|FD-Score:4.81|P-value:7.45E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YOL047C(YOL047C_p)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR022C(YPR022C_p)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:ACO1(YLR304C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ACO2(YJL200C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:AFT1(YGL071W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:CAF120(YNL278W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CDC10(YCR002C)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:ELP2(YGR200C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:FLD1(YLR404W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:GIS1(YDR096W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:HUB1(YNR032C-A)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:MRPL37(YBR268W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH3(YCR092C)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:PAM17(YKR065C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress Gene:PCI8(YIL071C)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PDR10(YOR328W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PEX12(YMR026C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:RDS1(YCR106W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RIM4(YHL024W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:SER1(YOR184W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SIP4(YJL089W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SIZ1(YDR409W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLS1(YLR139C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SMA1(YPL027W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SRL1(YOR247W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SUB1(YMR039C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:TAL1(YLR354C)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:UBP15(YMR304W)|FD-Score:-4.07|P-value:2.37E-5||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:YEA6(YEL006W)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YLL020C(YLL020C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR232W(YLR232W_d)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Gene:YMR253C(YMR253C_p)|FD-Score:4.81|P-value:7.45E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YOL047C(YOL047C_p)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR022C(YPR022C_p)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR186C4.318.09E-60.63PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YLR066W4.191.37E-50.63SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YDR398W3.561.86E-40.07UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YFR051C3.492.44E-40.52RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YPL153C2.960.001520.03RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YKL052C2.940.001660.07ASK1Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
YNL189W2.870.002080.08SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YKL012W2.780.002690.01PRP40U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex
YDR299W2.780.002750.05BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YML098W2.730.003170.01TAF13TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
YML077W2.720.003280.02BET5Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
YBL035C2.690.003530.01POL12B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
YJR013W2.690.003620.01GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YOR218C_d2.670.003770.02YOR218C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
YNL287W2.650.004060.05SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR253C_p4.817.45E-7YMR253C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
YLR232W_d4.221.22E-5YLR232W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5
YLR404W4.221.24E-5FLD1Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
YKR065C4.161.59E-5PAM17Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress
YDR409W3.924.39E-5SIZ1SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
YGL071W3.701.09E-4AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YLL020C_d3.631.40E-4YLL020C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1
YMR039C3.581.69E-4SUB1Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress
YOR034C-A_p3.561.87E-4YOR034C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YGR200C3.482.54E-4ELP2Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
YJL089W3.433.05E-4SIP4C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus
YNL278W3.393.45E-4CAF120Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication
YLR304C3.393.51E-4ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YJL200C3.344.16E-4ACO2Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
YPL027W3.314.63E-4SMA1Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p

GO enrichment analysis for SGTC_2143
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0978.79E-14SGTC_32309131665 49.5 μMChembridge (Drug-like library)159437200.0793651
0.0672.56E-7SGTC_5294076-0302 30.3 μMChemDiv (Drug-like library)58165220.05mitochondrial processes
0.0663.89E-7SGTC_28479005661 71.4 μMChembridge (Drug-like library)29914490.0735294
0.0621.63E-6SGTC_12700831-1170 17.3 μMChemDiv (Drug-like library)67647160.0714286
0.0622.13E-6SGTC_28869058944 52.0 μMChembridge (Drug-like library)49499290.0597015
0.0571.29E-5SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.0555556
0.0526.57E-5SGTC_2692st077426 47.7 μMTimTec (Natural product derivative library)252385710.0833333
0.0491.54E-4SGTC_1470k295-0292 231.0 μMChemDiv (Drug-like library)50456120.0789474
0.0482.60E-4SGTC_14514152-0210 104.0 μMChemDiv (Drug-like library)6595630.06
0.0472.79E-4SGTC_33429147791 14.5 μMChembridge (Drug-like library)18381680.0632911
0.0463.71E-4SGTC_10543429-0080 167.0 μMChemDiv (Drug-like library)11628770.246154
0.0463.71E-4SGTC_1946st076057 65.1 μMTimTec (Natural product derivative library)9530090.0857143
0.0463.84E-4SGTC_1938st074717 56.4 μMTimTec (Natural product derivative library)172509790.0657895
0.0463.84E-4SGTC_1530rhein 70.4 μMTimTec (Pure natural product library)101680.116667
0.0454.74E-4SGTC_21956536160 144.2 μMChembridge (Fragment library)38343860.052631660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21986589944200 μM0.6590915290268Chembridge (Fragment library)292.333521.73925
SGTC_2270792457590.91 μM0.321262135Chembridge (Fragment library)241.3050232.21414RPP1 & pyrimidine depletion
SGTC_18835-benzylidenerhodanine20 μM0.31251273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_24545-benzylidenerhodanine10.36 μM0.31251273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_20405100729123 μM0.31111111840Chembridge (Fragment library)161.157381.15612
SGTC_20545233173129 μM0.3111116889Chembridge (Fragment library)226.026861.41812mitochondrial response to ROS
SGTC_5231934-022213.7 μM0.3090911361595ChemDiv (Drug-like library)289.296653.78316
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.2884621241132ChemDiv (Drug-like library)227.32642.79414
SGTC_380929-005158.27 μM0.2884621379719ChemDiv (Drug-like library)223.2235432.14714
SGTC_2488526813545.7 μM0.2884621270356Miscellaneous247.335963.30913
SGTC_12840929-004316 μM0.2857141239883ChemDiv (Drug-like library)223.2235432.14714