5628481

N-(3,4-dimethylphenyl)-1,1-dioxo-2,3-dihydrothiophen-3-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2144
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 2818399
SMILES CC1=C(C=C(C=C1)NC2CS(=O)(=O)C=C2)C
Standardized SMILES Cc1ccc(NC2CS(=O)(=O)C=C2)cc1C
Molecular weight 237.318
ALogP 1.99
H-bond donor count 1
H-bond acceptor count 3
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.78
% growth inhibition (Hom. pool) 8.25


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2818399
Download HIP data (tab-delimited text)  (excel)
Gene:COG2(YGR120C)|FD-Score:-3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAD2(YKR083C)|FD-Score:4.2|P-value:1.32E-5|Clearance:0.63||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FCF2(YLR051C)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.14||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:MMS21(YEL019C)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:PSF3(YOL146W)|FD-Score:-3.25|P-value:5.84E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RIB7(YBR153W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.13||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:RPF1(YHR088W)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.63||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL15A(YLR029C)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.63||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:TCP1(YDR212W)|FD-Score:3.33|P-value:4.29E-4|Clearance:0.1||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:YDL196W(YDL196W_d)|FD-Score:3.97|P-value:3.63E-5|Clearance:0.63||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:COG2(YGR120C)|FD-Score:-3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAD2(YKR083C)|FD-Score:4.2|P-value:1.32E-5|Clearance:0.63||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FCF2(YLR051C)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.14||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:MMS21(YEL019C)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:PSF3(YOL146W)|FD-Score:-3.25|P-value:5.84E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RIB7(YBR153W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.13||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:RPF1(YHR088W)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.63||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL15A(YLR029C)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.63||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:TCP1(YDR212W)|FD-Score:3.33|P-value:4.29E-4|Clearance:0.1||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:YDL196W(YDL196W_d)|FD-Score:3.97|P-value:3.63E-5|Clearance:0.63||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2818399
Download HOP data (tab-delimited text)  (excel)
Gene:APL6(YGR261C)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APS3(YJL024C)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARL3(YPL051W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BEM3(YPL115C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:COA2(YPL189C-A)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CYB5(YNL111C)|FD-Score:6.26|P-value:1.90E-10||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:ECM34(YHL043W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:ERG2(YMR202W)|FD-Score:32.4|P-value:1.99E-230||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV41(YML067C)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FET3(YMR058W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:FMP32(YFL046W_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRK1(YPL141C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GEP7(YGL057C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GRR1(YJR090C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HER2(YMR293C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HOP2(YGL033W)|FD-Score:-3.95|P-value:3.86E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IDH2(YOR136W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IFA38(YBR159W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IMP2'(YIL154C)|FD-Score:12.6|P-value:1.25E-36||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP53(YOR109W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:MAC1(YMR021C)|FD-Score:5.69|P-value:6.20E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAK10(YEL053C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAL11(YGR289C)|FD-Score:-5.45|P-value:2.53E-8||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MET12(YPL023C)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MON2(YNL297C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRPL4(YLR439W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL8(YJL063C)|FD-Score:4.41|P-value:5.23E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:4.97|P-value:3.35E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:OAR1(YKL055C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OPI1(YHL020C)|FD-Score:4.4|P-value:5.44E-6||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PAH1(YMR165C)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR1(YGL013C)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PHO85(YPL031C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PIL1(YGR086C)|FD-Score:-3.8|P-value:7.38E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PRO2(YOR323C)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUS7(YOR243C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAV1(YJR033C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:ROT2(YBR229C)|FD-Score:4|P-value:3.20E-5||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPL15B(YMR121C)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPS18B(YML026C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM22(YKL155C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RSM7(YJR113C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAC1(YKL212W)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SDH2(YLL041C)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SDL1(YIL167W)|FD-Score:-3.79|P-value:7.41E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SFL1(YOR140W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SLM1(YIL105C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SLM5(YCR024C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SOD1(YJR104C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SRF1(YDL133W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SYS1(YJL004C)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TPN1(YGL186C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPP1(YMR156C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:VMA22(YHR060W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPH1(YOR270C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:WSS1(YHR134W)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAR047C(YAR047C_d)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR100C(YCR100C_p)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Putative protein of unknown function Gene:YDL118W(YDL118W_p)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL211C(YDL211C_p)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR203W(YDR203W_d)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR057W(YFR057W_p)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Putative protein of unknown function Gene:YGR219W(YGR219W_d)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YJL120W(YJL120W_d)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YML007C-A(YML007C-A_p)|FD-Score:8.14|P-value:2.02E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML020W(YML020W_p)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Putative protein of unknown function Gene:YMR099C(YMR099C)|FD-Score:9.81|P-value:5.13E-23||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YOR105W(YOR105W_p)|FD-Score:4|P-value:3.19E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YPK1(YKL126W)|FD-Score:5.01|P-value:2.67E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:APL6(YGR261C)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APS3(YJL024C)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARL3(YPL051W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BEM3(YPL115C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:COA2(YPL189C-A)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CYB5(YNL111C)|FD-Score:6.26|P-value:1.90E-10||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:ECM34(YHL043W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:ERG2(YMR202W)|FD-Score:32.4|P-value:1.99E-230||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV41(YML067C)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FET3(YMR058W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:FMP32(YFL046W_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRK1(YPL141C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GEP7(YGL057C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GRR1(YJR090C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HER2(YMR293C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HOP2(YGL033W)|FD-Score:-3.95|P-value:3.86E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IDH2(YOR136W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IFA38(YBR159W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IMP2'(YIL154C)|FD-Score:12.6|P-value:1.25E-36||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP53(YOR109W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:MAC1(YMR021C)|FD-Score:5.69|P-value:6.20E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAK10(YEL053C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAL11(YGR289C)|FD-Score:-5.45|P-value:2.53E-8||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MET12(YPL023C)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MON2(YNL297C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRPL4(YLR439W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPL8(YJL063C)|FD-Score:4.41|P-value:5.23E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:4.97|P-value:3.35E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:OAR1(YKL055C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OPI1(YHL020C)|FD-Score:4.4|P-value:5.44E-6||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PAH1(YMR165C)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PDR1(YGL013C)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PHO85(YPL031C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PIL1(YGR086C)|FD-Score:-3.8|P-value:7.38E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PRO2(YOR323C)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUS7(YOR243C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAV1(YJR033C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:ROT2(YBR229C)|FD-Score:4|P-value:3.20E-5||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPL15B(YMR121C)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPS18B(YML026C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM22(YKL155C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RSM7(YJR113C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAC1(YKL212W)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SDH2(YLL041C)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SDL1(YIL167W)|FD-Score:-3.79|P-value:7.41E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SFL1(YOR140W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SLM1(YIL105C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SLM5(YCR024C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SOD1(YJR104C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SRF1(YDL133W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SYS1(YJL004C)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TPN1(YGL186C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPP1(YMR156C)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:VMA22(YHR060W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPH1(YOR270C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:WSS1(YHR134W)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YAR047C(YAR047C_d)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR100C(YCR100C_p)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Putative protein of unknown function Gene:YDL118W(YDL118W_p)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL211C(YDL211C_p)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR203W(YDR203W_d)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR057W(YFR057W_p)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Putative protein of unknown function Gene:YGR219W(YGR219W_d)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YJL120W(YJL120W_d)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YML007C-A(YML007C-A_p)|FD-Score:8.14|P-value:2.02E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML020W(YML020W_p)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Putative protein of unknown function Gene:YMR099C(YMR099C)|FD-Score:9.81|P-value:5.13E-23||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YOR105W(YOR105W_p)|FD-Score:4|P-value:3.19E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YPK1(YKL126W)|FD-Score:5.01|P-value:2.67E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR088W4.241.14E-50.63RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YKR083C4.201.32E-50.63DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YLR029C4.121.89E-50.63RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YDL196W_d3.973.63E-50.63YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YDR212W3.334.29E-40.10TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YBR153W3.236.15E-40.13RIB7Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
YLR051C3.119.47E-40.14FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YAL038W2.960.001510.07CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YDL132W2.890.001910.03CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YNL110C2.870.002080.05NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YHR170W2.820.002390.05NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YMR281W2.770.002810.04GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YBR155W2.730.003160.17CNS1TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
YPR175W2.570.005150.01DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YOR326W2.560.005260.04MYO2Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W32.401.99E-230ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YIL154C12.601.25E-36IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YMR099C9.815.13E-23YMR099CGlucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
YML007C-A_p8.142.02E-16YML007C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
YNL111C6.261.90E-10CYB5Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
YMR021C5.696.20E-9MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YPL023C5.171.18E-7MET12Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR
YAR047C_d5.161.21E-7YAR047C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL212W5.131.43E-7SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YKL126W5.012.67E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YMR158W4.973.35E-7MRPS8Mitochondrial ribosomal protein of the small subunit
YOR323C4.865.94E-7PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YBR069C4.661.61E-6TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YJL004C4.444.52E-6SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YJL063C4.415.23E-6MRPL8Mitochondrial ribosomal protein of the large subunit

GO enrichment analysis for SGTC_2144
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4940SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.084507ERG2
0.4086.68E-236SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0851064ERG2
0.3994.32E-224SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0227273calcium & mitochondrial duress
0.3937.41E-217SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.057971ERG2
0.3861.48E-209SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.117647ERG2
0.3852.25E-208SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.186441ERG2
0.3682.13E-189SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0545455ERG2
0.3585.98E-178SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.0724638ERG2
0.3514.67E-170SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.0657895ERG2
0.3481.00E-167SGTC_23007976697 71.6 μMChembridge (Fragment library)22361090.0862069ERG2
0.3411.86E-160SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.163636ERG2
0.3405.33E-160SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.0779221ERG2
0.3274.06E-147SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.0833333calcium & mitochondrial duress
0.3241.07E-144SGTC_12470448-0043 5.8 μMChemDiv (Drug-like library)30890130.0806452ERG2
0.3211.51E-141SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.0821918ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_23819035592200 μM0.4651164749657Chembridge (Fragment library)243.70991.68313
SGTC_10034049-0218100 μM0.319149295161ChemDiv (Drug-like library)279.3298633.49514
SGTC_21005377183190 μM0.3137262844202Chembridge (Fragment library)250.316762.6631460S ribosome export
SGTC_2138557559377.67 μM0.304348762213Chembridge (Fragment library)212.290243.04412
SGTC_3186911046449.47 μM0.29411817018021Chembridge (Drug-like library)269.338264.05612RPP1 & pyrimidine depletion
SGTC_2957907680011.52 μM0.28333316459615Chembridge (Drug-like library)290.357482.18614
SGTC_21195528445120.64 μM0.28791935Chembridge (Fragment library)212.290243.04412
SGTC_215856571233.62 μM0.262858827Chembridge (Fragment library)269.29521.81603
SGTC_11770983-020423.6 μM0.254545766818ChemDiv (Drug-like library)318.369123.99613
SGTC_14294049-02059.46 μM0.22641522774478ChemDiv (Drug-like library)344.1993433.75714