5634607

6-(2,5-dimethylanilino)-1H-pyrimidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2145
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 308662
SMILES CC1=CC(=C(C=C1)C)NC2=CC(=O)NC(=O)N2
Standardized SMILES Cc1ccc(C)c(Nc2cc(O)nc(O)n2)c1
Molecular weight 231.2505
ALogP 2.03
H-bond donor count 3
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.07
% growth inhibition (Hom. pool) -1.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 308662
Download HIP data (tab-delimited text)  (excel)
Gene:FCP1(YMR277W)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:RIX1(YHR197W)|FD-Score:5.3|P-value:5.79E-8|Clearance:1.09||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPP0(YLR340W)|FD-Score:3.18|P-value:7.43E-4|Clearance:0.34||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPS15(YOL040C)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.38||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS31(YLR167W)|FD-Score:4.88|P-value:5.23E-7|Clearance:1.09||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RSC9(YML127W)|FD-Score:-3.1|P-value:9.52E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:YDL163W(YDL163W_d)|FD-Score:-4.37|P-value:6.33E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YLR339C(YLR339C_d)|FD-Score:3.8|P-value:7.33E-5|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:FCP1(YMR277W)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:RIX1(YHR197W)|FD-Score:5.3|P-value:5.79E-8|Clearance:1.09||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPP0(YLR340W)|FD-Score:3.18|P-value:7.43E-4|Clearance:0.34||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPS15(YOL040C)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.38||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS31(YLR167W)|FD-Score:4.88|P-value:5.23E-7|Clearance:1.09||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RSC9(YML127W)|FD-Score:-3.1|P-value:9.52E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:YDL163W(YDL163W_d)|FD-Score:-4.37|P-value:6.33E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YLR339C(YLR339C_d)|FD-Score:3.8|P-value:7.33E-5|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 308662
Download HOP data (tab-delimited text)  (excel)
Gene:ACA1(YER045C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:APE2(YKL157W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:COG1(YGL223C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRN1(YLR429W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CRS5(YOR031W)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DIT1(YDR403W)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ECM34(YHL043W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:FUR4(YBR021W)|FD-Score:5.37|P-value:3.89E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GTT1(YIR038C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:GUD1(YDL238C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:GUP1(YGL084C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HOF1(YMR032W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IES4(YOR189W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IGO2(YHR132W-A)|FD-Score:-3.97|P-value:3.54E-5||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:IRC21(YMR073C)|FD-Score:4.44|P-value:4.47E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LYS4(YDR234W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MDM20(YOL076W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MDY2(YOL111C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MET1(YKR069W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MIP1(YOR330C)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MMT2(YPL224C)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MOG1(YJR074W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:OPI3(YJR073C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAN2(YGL094C)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDR1(YGL013C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PLB3(YOL011W)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMP1(YCR024C-A)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RPL38(YLR325C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RSC2(YLR357W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RSM7(YJR113C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SGM1(YJR134C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SNT309(YPR101W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SNZ2(YNL333W)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SRV2(YNL138W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:TDA5(YLR426W_p)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TGL2(YDR058C)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:VOA1(YGR106C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:XDJ1(YLR090W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YAL037C-A(YAL037C-A_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function Gene:YJR056C(YJR056C_p)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YML108W(YML108W_p)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YTA7(YGR270W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ACA1(YER045C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:APE2(YKL157W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:COG1(YGL223C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRN1(YLR429W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CRS5(YOR031W)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DIT1(YDR403W)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ECM34(YHL043W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:FUR4(YBR021W)|FD-Score:5.37|P-value:3.89E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GTT1(YIR038C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:GUD1(YDL238C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:GUP1(YGL084C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HOF1(YMR032W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IES4(YOR189W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IGO2(YHR132W-A)|FD-Score:-3.97|P-value:3.54E-5||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:IRC21(YMR073C)|FD-Score:4.44|P-value:4.47E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LYS4(YDR234W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MDM20(YOL076W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MDY2(YOL111C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MET1(YKR069W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MIP1(YOR330C)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MMT2(YPL224C)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MOG1(YJR074W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:OPI3(YJR073C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAN2(YGL094C)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDR1(YGL013C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PLB3(YOL011W)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMP1(YCR024C-A)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RPL38(YLR325C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RSC2(YLR357W)|FD-Score:4.01|P-value:3.05E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RSM7(YJR113C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SGM1(YJR134C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SNT309(YPR101W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SNZ2(YNL333W)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SRV2(YNL138W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:TDA5(YLR426W_p)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TGL2(YDR058C)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:VOA1(YGR106C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:XDJ1(YLR090W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YAL037C-A(YAL037C-A_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function Gene:YJR056C(YJR056C_p)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YML108W(YML108W_p)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YTA7(YGR270W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR197W5.305.79E-81.09RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YLR167W4.885.23E-71.09RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YLR339C_d3.807.33E-50.24YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
YOL040C3.561.86E-40.38RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YLR340W3.187.43E-40.34RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YCL054W2.830.002290.07SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YKL112W2.770.002810.05ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YGR186W2.710.003320.06TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YDL028C2.660.003950.06MPS1Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
YJL090C2.590.004760.03DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YDL084W2.570.005140.06SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDR301W2.510.006060.02CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YDL235C2.490.006340.01YPD1Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
YPL128C2.480.006620.00TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YPR133C2.470.006690.02SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR021W5.373.89E-8FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YDL238C4.721.20E-6GUD1Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
YMR073C4.444.47E-6IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YLR426W_p4.072.35E-5TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YLR357W4.013.05E-5RSC2Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance
YDR058C3.914.53E-5TGL2Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli
YLR325C3.904.89E-5RPL38Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog
YDR403W3.807.38E-5DIT1Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure
YGL094C3.787.71E-5PAN2Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
YJR074W3.729.79E-5MOG1Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
YAL037C-A_p3.601.59E-4YAL037C-A_pPutative protein of unknown function
YGR270W3.581.75E-4YTA7Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p
YGL084C3.571.76E-4GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YPR101W3.492.41E-4SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
YNL138W3.492.42E-4SRV2CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis

GO enrichment analysis for SGTC_2145
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1219.47E-21SGTC_21385575593 77.7 μMChembridge (Fragment library)7622130.176471
0.1132.94E-18SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.185185RNA processing & uracil transport
0.1095.16E-17SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.185185RNA processing & uracil transport
0.0899.42E-12SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.140351RNA processing & uracil transport
0.0863.59E-11SGTC_1167k781-0610 148.0 μMChemDiv (Drug-like library)45251690.0923077RNA processing & uracil transport
0.0855.51E-11SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.163934PDR1
0.0848.46E-11SGTC_22977971671 152.3 μMChembridge (Fragment library)9090160.111111RNA processing & uracil transport
0.0822.51E-10SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.0704225RNA processing & uracil transport
0.0807.81E-10SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.0757576PDR1
0.0781.70E-9SGTC_1220236-0016 402.4 μMChemDiv (Drug-like library)68088380.103896RNA processing & uracil transport
0.0782.34E-9SGTC_30029075603 71.4 μMChembridge (Drug-like library)201054050.0694444
0.0773.89E-9SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.112903RNA processing & uracil transport
0.0765.38E-9SGTC_1817st052116 64.4 μMTimTec (Natural product derivative library)43613980.2RNA processing & uracil transport
0.0741.53E-8SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0853659PDR1
0.0731.59E-8SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0555556PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12212958-7315257 μM0.3958334562705ChemDiv (Drug-like library)285.340963.24633
SGTC_21175521686200 μM0.313726803312Chembridge (Fragment library)227.261821.71413
SGTC_21185526350200 μM0.306122743594Chembridge (Fragment library)212.290243.25912
SGTC_20405100729123 μM0.30232611840Chembridge (Fragment library)161.157381.15612
SGTC_13311472-004976.2 μM0.29090924051409ChemDiv (Drug-like library)286.325723.57824
SGTC_2041543-018462.41 μM0.2698412252213ChemDiv (Drug-like library)356.4138834.76515TRP & mitochondrial translation
SGTC_1881565248416 μM0.2656252255129Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_9671306-0166352 μM0.2545454437607ChemDiv (Drug-like library)246.265163.37524
SGTC_2840988-010432.6 μM0.254666532ChemDiv (Drug-like library)329.3525.28314heme requiring
SGTC_1853st05671091.2 μM0.25269167TimTec (Natural product derivative library)219.236521.66613amide catabolism