5545642

2-oxo-6-thiophen-2-yl-1H-pyridine-3-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2146
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 825326
SMILES C1=CSC(=C1)C2=CC=C(C(=O)N2)C#N
Standardized SMILES Oc1nc(ccc1C#N)c2cccs2
Molecular weight 202.2324
ALogP 1.22
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 23.14
% growth inhibition (Hom. pool) -5.09


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 825326
Download HIP data (tab-delimited text)  (excel)
Gene:BIG1(YHR101C)|FD-Score:-3.93|P-value:4.18E-5|Clearance:0||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:CCT3(YJL014W)|FD-Score:3.47|P-value:2.61E-4|Clearance:0.2||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GCD10(YNL062C)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.1||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:IMP3(YHR148W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.01||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:PDI1(YCL043C)|FD-Score:3.96|P-value:3.71E-5|Clearance:0.26||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PRP3(YDR473C)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.09||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB5(YBR154C)|FD-Score:3.1|P-value:9.54E-4|Clearance:0.1||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC11(YDR045C)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RRP46(YGR095C)|FD-Score:3.15|P-value:8.06E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP9(YPR137W)|FD-Score:3.83|P-value:6.51E-5|Clearance:0.26||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RTT105(YER104W)|FD-Score:-5.84|P-value:2.68E-9|Clearance:0||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:UTP6(YDR449C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.02||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:BIG1(YHR101C)|FD-Score:-3.93|P-value:4.18E-5|Clearance:0||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:CCT3(YJL014W)|FD-Score:3.47|P-value:2.61E-4|Clearance:0.2||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GCD10(YNL062C)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.1||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:IMP3(YHR148W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.01||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:PDI1(YCL043C)|FD-Score:3.96|P-value:3.71E-5|Clearance:0.26||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PRP3(YDR473C)|FD-Score:3.56|P-value:1.83E-4|Clearance:0.09||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB5(YBR154C)|FD-Score:3.1|P-value:9.54E-4|Clearance:0.1||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC11(YDR045C)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RRP46(YGR095C)|FD-Score:3.15|P-value:8.06E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP9(YPR137W)|FD-Score:3.83|P-value:6.51E-5|Clearance:0.26||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RTT105(YER104W)|FD-Score:-5.84|P-value:2.68E-9|Clearance:0||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:UTP6(YDR449C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.02||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 825326
Download HOP data (tab-delimited text)  (excel)
Gene:AHT1(YHR093W_d)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ATX1(YNL259C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:AVT1(YJR001W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BIO4(YNR057C)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BOI1(YBL085W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BUD14(YAR014C)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:BUD21(YOR078W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:BXI1(YNL305C)|FD-Score:-4.35|P-value:6.96E-6||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CAM1(YPL048W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CBP1(YJL209W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCH1(YGR217W)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:COA1(YIL157C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:DDI3(YNL335W_p)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DID4(YKL002W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DSE4(YNR067C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:ETR1(YBR026C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:EXG1(YLR300W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FYV6(YNL133C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAD1(YMR250W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCN20(YFR009W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GFD2(YCL036W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GNT1(YOR320C)|FD-Score:-6.39|P-value:8.14E-11||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:GRX4(YER174C)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HBN1(YCL026C-B_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HSP82(YPL240C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:MDG1(YNL173C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MRPL15(YLR312W-A)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MTC7(YEL033W_p)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NAT1(YDL040C)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NUP157(YER105C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication Gene:OAC1(YKL120W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:PEP7(YDR323C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO85(YPL031C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PLB3(YOL011W)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PRY2(YKR013W)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PSY3(YLR376C)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:QCR2(YPR191W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:REV3(YPL167C)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RHB1(YCR027C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RIT1(YMR283C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPS23B(YPR132W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS9A(YPL081W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RTT109(YLL002W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SEH1(YGL100W)|FD-Score:4.79|P-value:8.29E-7||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SMI1(YGR229C)|FD-Score:6.21|P-value:2.66E-10||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPE3(YPR069C)|FD-Score:7.73|P-value:5.56E-15||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SVL3(YPL032C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TMA17(YDL110C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:TOM5(YPR133W-A)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:VOA1(YGR106C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS21(YOR089C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:XBP1(YIL101C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL036C(YBL036C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS Gene:YCL001W-A(YCL001W-A_p)|FD-Score:5.29|P-value:6.24E-8||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR075W-A(YCR075W-A_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL050C(YDL050C_d)|FD-Score:-3.73|P-value:9.56E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHL026C(YHL026C_p)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YHR182W(YHR182W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YHR202W(YHR202W_p)|FD-Score:5.21|P-value:9.63E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJL119C(YJL119C_d)|FD-Score:-3.86|P-value:5.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKE2(YLR200W)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL115C(YKL115C_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKR047W(YKR047W_d)|FD-Score:5.43|P-value:2.89E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YMR194C-A(YMR194C-A_d)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOR105W(YOR105W_p)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YPK3(YBR028C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL067C(YPL067C_p)|FD-Score:-3.73|P-value:9.57E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL264C(YPL264C_p)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:AHT1(YHR093W_d)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ATX1(YNL259C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:AVT1(YJR001W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BIO4(YNR057C)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BOI1(YBL085W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication Gene:BUD14(YAR014C)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:BUD21(YOR078W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:BXI1(YNL305C)|FD-Score:-4.35|P-value:6.96E-6||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CAM1(YPL048W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CBP1(YJL209W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCH1(YGR217W)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:COA1(YIL157C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:DDI3(YNL335W_p)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DID4(YKL002W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DSE4(YNR067C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:ETR1(YBR026C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:EXG1(YLR300W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FYV6(YNL133C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAD1(YMR250W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GCN20(YFR009W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GFD2(YCL036W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GNT1(YOR320C)|FD-Score:-6.39|P-value:8.14E-11||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:GRX4(YER174C)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HBN1(YCL026C-B_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HSP82(YPL240C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:MDG1(YNL173C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MRPL15(YLR312W-A)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MTC7(YEL033W_p)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NAT1(YDL040C)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NUP157(YER105C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication Gene:OAC1(YKL120W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:PEP7(YDR323C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO85(YPL031C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PLB3(YOL011W)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PRY2(YKR013W)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PSR1(YLL010C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PSY3(YLR376C)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:QCR2(YPR191W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:REV3(YPL167C)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RHB1(YCR027C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RIT1(YMR283C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPS23B(YPR132W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS9A(YPL081W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RTT109(YLL002W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SEH1(YGL100W)|FD-Score:4.79|P-value:8.29E-7||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SMI1(YGR229C)|FD-Score:6.21|P-value:2.66E-10||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPE3(YPR069C)|FD-Score:7.73|P-value:5.56E-15||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SVL3(YPL032C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TMA17(YDL110C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion Gene:TOM5(YPR133W-A)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:VOA1(YGR106C)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS21(YOR089C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:XBP1(YIL101C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL036C(YBL036C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS Gene:YCL001W-A(YCL001W-A_p)|FD-Score:5.29|P-value:6.24E-8||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR075W-A(YCR075W-A_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL050C(YDL050C_d)|FD-Score:-3.73|P-value:9.56E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHL026C(YHL026C_p)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YHR182W(YHR182W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YHR202W(YHR202W_p)|FD-Score:5.21|P-value:9.63E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJL119C(YJL119C_d)|FD-Score:-3.86|P-value:5.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKE2(YLR200W)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL115C(YKL115C_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKR047W(YKR047W_d)|FD-Score:5.43|P-value:2.89E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YMR194C-A(YMR194C-A_d)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOR105W(YOR105W_p)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YPK3(YBR028C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL067C(YPL067C_p)|FD-Score:-3.73|P-value:9.57E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL264C(YPL264C_p)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCL043C3.963.71E-50.26PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YPR137W3.836.51E-50.26RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YDR473C3.561.83E-40.09PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YHR148W3.482.54E-40.01IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
YJL014W3.472.61E-40.20CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR449C3.275.42E-40.02UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YNL062C3.255.73E-40.10GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YGR095C3.158.06E-40.05RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YBR154C3.109.54E-40.10RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YKL112W3.010.001320.04ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YOL021C2.970.001480.02DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YDR429C2.950.001580.12TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YKL144C2.830.002320.08RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YNL075W2.750.002940.13IMP4Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
YBR236C2.630.004290.03ABD1Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR069C7.735.56E-15SPE3Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
YGR229C6.212.66E-10SMI1Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
YKR047W_d5.432.89E-8YKR047W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1
YCL001W-A_p5.296.24E-8YCL001W-A_pPutative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III
YHR202W_p5.219.63E-8YHR202W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
YGL100W4.798.29E-7SEH1Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1
YDL040C4.356.75E-6NAT1Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
YPR191W4.251.06E-5QCR2Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme
YMR250W4.221.22E-5GAD1Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
YEL033W_p4.211.29E-5MTC7_pPredicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
YPL031C4.181.45E-5PHO85Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle
YOR078W4.161.61E-5BUD21Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
YGR106C4.161.62E-5VOA1Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval
YFR009W4.111.99E-5GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YLL010C4.052.61E-5PSR1Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p

GO enrichment analysis for SGTC_2146
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0791.02E-9SGTC_32299132204 49.5 μMChembridge (Drug-like library)176829240.0759494
0.0681.38E-7SGTC_11871391-0724 117.0 μMChemDiv (Drug-like library)69979860.0657895amide catabolism
0.0604.45E-6SGTC_29035923464 63.9 μMChembridge (Drug-like library)28765760.0571429
0.0596.54E-6SGTC_6173770-0098 35.0 μMChemDiv (Drug-like library)59626390.0833333exosome
0.0588.89E-6SGTC_32649137442 49.5 μMChembridge (Drug-like library)170157260.0704225
0.0561.86E-5SGTC_14843473-2379 286.0 μMChemDiv (Drug-like library)6719170.0895522
0.0552.66E-5SGTC_21485546263 113.6 μMChembridge (Fragment library)7920970.05
0.0526.23E-5SGTC_30359091303 49.5 μMChembridge (Drug-like library)252370020.0625
0.0526.90E-5SGTC_9143820-6779 408.0 μMChemDiv (Drug-like library)28934810.177419
0.0519.59E-5SGTC_2593nobiletin 86.2 μMMicrosource (Natural product library)723440.078125
0.0501.10E-4SGTC_6200109-0052 432.0 μMChemDiv (Drug-like library)4930370.057971
0.0501.24E-4SGTC_7150868-0130 184.0 μMChemDiv (Drug-like library)28762580.0684932
0.0491.69E-4SGTC_6961560-0013 611.0 μMChemDiv (Drug-like library)16502690.0461538
0.0482.17E-4SGTC_32759138069 49.5 μMChembridge (Drug-like library)176170070.0847458
0.0464.15E-4SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.0810811translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21365328288199.3 μM0.311111262395Chembridge (Fragment library)222.220540.81724
SGTC_2065522159634.26 μM0.297872788581Chembridge (Fragment library)238.286141.71724
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.281241132ChemDiv (Drug-like library)227.32642.79414
SGTC_20825219693150 μM0.274512834649Chembridge (Fragment library)236.228820.44424RNA processing & uracil transport
SGTC_2276794751342.08 μM0.274512105100Chembridge (Fragment library)234.297442.23314
SGTC_5554296-068537 μM0.2741942908540ChemDiv (Drug-like library)292.419643.11714ergosterol biosynthesis
SGTC_2071523095139.92 μM0.2653061799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4
SGTC_22087283366200 μM0.265306898663Chembridge (Fragment library)224.302621.34324
SGTC_6721441-001853.7 μM0.255319251141ChemDiv (Drug-like library)212.270323.19403
SGTC_1982st07246771.9 μM0.2545453163533TimTec (Natural product derivative library)278.32513.99803