5545932

2-methyl-1-(4-phenylpiperazin-1-yl)propan-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2147
Screen concentration 89.7 μM
Source Chembridge (Fragment library)
PubChem CID 797404
SMILES CC(C)C(=O)N1CCN(CC1)C2=CC=CC=C2
Standardized SMILES CC(C)C(=O)N1CCN(CC1)c2ccccc2
Molecular weight 232.3214
ALogP 2.34
H-bond donor count 0
H-bond acceptor count 2
Response signature ergosterol biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.71
% growth inhibition (Hom. pool) 3.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 797404
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:5.91|P-value:1.68E-9|Clearance:0.75||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CAB3(YKL088W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.07||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:DAD1(YDR016C)|FD-Score:3.25|P-value:5.75E-4|Clearance:0.01||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG11(YHR007C)|FD-Score:6.78|P-value:5.95E-12|Clearance:0.75||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:6.58|P-value:2.41E-11|Clearance:0.75||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:HSH49(YOR319W)|FD-Score:6.69|P-value:1.11E-11|Clearance:0.75||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSM2(YBL026W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.29||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NOP53(YPL146C)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.02||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PGS1(YCL004W)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.01||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PZF1(YPR186C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.07||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RAT1(YOR048C)|FD-Score:5.96|P-value:1.28E-9|Clearance:0.75||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RFC1(YOR217W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.11||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIX7(YLL034C)|FD-Score:3.1|P-value:9.80E-4|Clearance:0.07||SGD DESC:Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions Gene:RNA15(YGL044C)|FD-Score:4.54|P-value:2.75E-6|Clearance:0.18||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB5(YBR154C)|FD-Score:4.76|P-value:9.67E-7|Clearance:0.22||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC17(YJL011C)|FD-Score:3.43|P-value:3.06E-4|Clearance:0.08||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:SAD1(YFR005C)|FD-Score:4.36|P-value:6.50E-6|Clearance:0.04||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SFT1(YKL006C-A)|FD-Score:4.22|P-value:1.22E-5|Clearance:0.15||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SPP2(YOR148C)|FD-Score:3.96|P-value:3.82E-5|Clearance:0.24||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SRP1(YNL189W)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.02||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TAF11(YML015C)|FD-Score:5.16|P-value:1.23E-7|Clearance:0.4||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:TIF35(YDR429C)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.05||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOA1(YOR194C)|FD-Score:4.32|P-value:7.97E-6|Clearance:0.1||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:BMS1(YPL217C)|FD-Score:5.91|P-value:1.68E-9|Clearance:0.75||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CAB3(YKL088W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.07||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:DAD1(YDR016C)|FD-Score:3.25|P-value:5.75E-4|Clearance:0.01||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG11(YHR007C)|FD-Score:6.78|P-value:5.95E-12|Clearance:0.75||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:6.58|P-value:2.41E-11|Clearance:0.75||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:HSH49(YOR319W)|FD-Score:6.69|P-value:1.11E-11|Clearance:0.75||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSM2(YBL026W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.29||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NOP53(YPL146C)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.02||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PGS1(YCL004W)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.01||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PZF1(YPR186C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.07||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RAT1(YOR048C)|FD-Score:5.96|P-value:1.28E-9|Clearance:0.75||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RFC1(YOR217W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.11||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIX7(YLL034C)|FD-Score:3.1|P-value:9.80E-4|Clearance:0.07||SGD DESC:Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions Gene:RNA15(YGL044C)|FD-Score:4.54|P-value:2.75E-6|Clearance:0.18||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB5(YBR154C)|FD-Score:4.76|P-value:9.67E-7|Clearance:0.22||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC17(YJL011C)|FD-Score:3.43|P-value:3.06E-4|Clearance:0.08||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:SAD1(YFR005C)|FD-Score:4.36|P-value:6.50E-6|Clearance:0.04||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SFT1(YKL006C-A)|FD-Score:4.22|P-value:1.22E-5|Clearance:0.15||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SPP2(YOR148C)|FD-Score:3.96|P-value:3.82E-5|Clearance:0.24||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SRP1(YNL189W)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.02||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TAF11(YML015C)|FD-Score:5.16|P-value:1.23E-7|Clearance:0.4||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:TIF35(YDR429C)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.05||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOA1(YOR194C)|FD-Score:4.32|P-value:7.97E-6|Clearance:0.1||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 797404
Download HOP data (tab-delimited text)  (excel)
Gene:AGC1(YPR021C)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AIM44(YPL158C)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALG6(YOR002W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ALG8(YOR067C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:AVT1(YJR001W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAP2(YIL034C)|FD-Score:6.11|P-value:4.98E-10||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CTP1(YBR291C)|FD-Score:9.01|P-value:1.02E-19||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:DOA1(YKL213C)|FD-Score:-3.92|P-value:4.39E-5||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:EMI2(YDR516C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:FAA4(YMR246W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:GAT4(YIR013C)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GCN5(YGR252W)|FD-Score:5.68|P-value:6.75E-9||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GID7(YCL039W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GMC2(YLR445W)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GUD1(YDL238C)|FD-Score:4.8|P-value:7.74E-7||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HMG1(YML075C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:ILM1(YJR118C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IML2(YJL082W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:LAG2(YOL025W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LIP2(YLR239C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MDG1(YNL173C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MFG1(YDL233W_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MPT5(YGL178W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity Gene:NDL1(YLR254C)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NEM1(YHR004C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:PAD1(YDR538W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PHO13(YDL236W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:RGP1(YDR137W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-5.26|P-value:7.17E-8||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL31B(YLR406C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:SHC1(YER096W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SNX4(YJL036W)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:STI1(YOR027W)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:UTH1(YKR042W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VPS17(YOR132W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS61(YDR136C_d)|FD-Score:-5.28|P-value:6.51E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-5.67|P-value:7.18E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:WSC2(YNL283C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YAR028W(YAR028W_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YCR101C(YCR101C_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDC1(YPL087W)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL034W(YDL034W_d)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDR154C(YDR154C_d)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIL028W(YIL028W_d)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YKL107W(YKL107W_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML034C-A(YML034C-A_d)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL193W(YNL193W_p)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YPR127W(YPR127W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPT6(YLR262C)|FD-Score:-4.56|P-value:2.61E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSP1(YHR155W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:AGC1(YPR021C)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AIM44(YPL158C)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALG6(YOR002W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ALG8(YOR067C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:AVT1(YJR001W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAP2(YIL034C)|FD-Score:6.11|P-value:4.98E-10||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CTP1(YBR291C)|FD-Score:9.01|P-value:1.02E-19||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:DOA1(YKL213C)|FD-Score:-3.92|P-value:4.39E-5||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:EMI2(YDR516C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:FAA4(YMR246W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:GAT4(YIR013C)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GCN5(YGR252W)|FD-Score:5.68|P-value:6.75E-9||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GID7(YCL039W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GMC2(YLR445W)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GUD1(YDL238C)|FD-Score:4.8|P-value:7.74E-7||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HMG1(YML075C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:ILM1(YJR118C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IML2(YJL082W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:LAG2(YOL025W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LIP2(YLR239C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MDG1(YNL173C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MFG1(YDL233W_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene Gene:MPT5(YGL178W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity Gene:NDL1(YLR254C)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NEM1(YHR004C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:PAD1(YDR538W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PHO13(YDL236W)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:RGP1(YDR137W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-5.26|P-value:7.17E-8||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL31B(YLR406C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:SHC1(YER096W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SNX4(YJL036W)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:STI1(YOR027W)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:UTH1(YKR042W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VPS17(YOR132W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS61(YDR136C_d)|FD-Score:-5.28|P-value:6.51E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-5.67|P-value:7.18E-9||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:WSC2(YNL283C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YAR028W(YAR028W_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YCR101C(YCR101C_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDC1(YPL087W)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL034W(YDL034W_d)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDR154C(YDR154C_d)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIL028W(YIL028W_d)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YKL107W(YKL107W_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML034C-A(YML034C-A_d)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL193W(YNL193W_p)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YPR127W(YPR127W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPT6(YLR262C)|FD-Score:-4.56|P-value:2.61E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSP1(YHR155W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR007C6.785.95E-120.75ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YOR319W6.691.11E-110.75HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YHR072W6.582.41E-110.75ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YOR048C5.961.28E-90.75RAT1Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
YPL217C5.911.68E-90.75BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YML015C5.161.23E-70.40TAF11TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
YBR154C4.769.67E-70.22RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YGL044C4.542.75E-60.18RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YFR005C4.366.50E-60.04SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YOR194C4.327.97E-60.10TOA1TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
YKL006C-A4.221.22E-50.15SFT1Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment
YOR217W4.072.34E-50.12RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YOR148C3.963.82E-50.24SPP2Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
YBL026W3.711.02E-40.29LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YJL011C3.433.06E-40.08RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR291C9.011.02E-19CTP1Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family
YIL034C6.114.98E-10CAP2Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
YGR252W5.686.75E-9GCN5Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
YDL238C4.807.74E-7GUD1Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
YLR239C4.741.05E-6LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YMR246W4.741.06E-6FAA4Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication
YLR406C4.741.07E-6RPL31BRibosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
YJR118C4.211.28E-5ILM1Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
YNL283C4.141.75E-5WSC2Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
YPL158C4.042.71E-5AIM44Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress
YJL036W3.993.28E-5SNX4Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p
YKR042W3.944.10E-5UTH1Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
YOR067C3.885.14E-5ALG8Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
YLR445W3.885.16E-5GMC2Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor
YPR021C3.797.63E-5AGC1Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis

GO enrichment analysis for SGTC_2147
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2282.24E-70SGTC_21555570423 76.0 μMChembridge (Fragment library)7974020.705882ergosterol biosynthesis
0.2217.37E-66SGTC_21856113692 200.0 μMChembridge (Fragment library)8741500.395349ergosterol biosynthesis
0.1951.52E-51SGTC_23779073351 62.6 μMChembridge (Fragment library)73379460.488372ergosterol biosynthesis
0.1905.46E-49SGTC_1638st008370 60.6 μMTimTec (Natural product derivative library)242071340.186441ergosterol biosynthesis
0.1848.55E-46SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0847458ergosterol biosynthesis
0.1771.60E-42SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride 61.8 μMTimTec (Natural product derivative library)170377580.206897ergosterol biosynthesis
0.1751.02E-41SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.348837ergosterol biosynthesis
0.1654.78E-37SGTC_1547harmine 94.2 μMMiscellaneous52809530.0701754ergosterol biosynthesis
0.1575.39E-34SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.157895azole & statin
0.1554.59E-33SGTC_2525harmine 47.9 μMMiscellaneous52809530.0701754ergosterol biosynthesis
0.1262.39E-22SGTC_1097itavastatin 7.2 μMNIH Clinical Collection52824510.125azole & statin
0.1202.70E-20SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.132075ergosterol biosynthesis
0.1193.57E-20SGTC_2753fluvastatin 17.1 μMMiscellaneous4461550.157143azole & statin
0.1181.14E-19SGTC_120849-0099 9.3 μMChemDiv (Drug-like library)60713880.131148
0.1102.14E-17SGTC_29749094440 9.7 μMChembridge (Drug-like library)252361340.15873ergosterol biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2155557042376 μM0.705882797402Chembridge (Fragment library)250.3118632.54403ergosterol biosynthesis
SGTC_20204017018200 μM0.51428621127491Chembridge (Fragment library)320.36530.23957
SGTC_2377907335162.6 μM0.4883727337946Chembridge (Fragment library)281.78112.25613ergosterol biosynthesis
SGTC_23849036245200 μM0.4523816462766Chembridge (Fragment library)261.362621.83713
SGTC_3056909490049.47 μM0.425236784Chembridge (Drug-like library)342.4072233.88404
SGTC_21856113692200 μM0.395349874150Chembridge (Fragment library)250.3118632.53703ergosterol biosynthesis
SGTC_3128912769149.47 μM0.39215741316314Chembridge (Drug-like library)338.426722.32815
SGTC_1779st03942755 μM0.3888891124498TimTec (Natural product derivative library)363.45283.50103
SGTC_3150909757449.47 μM0.3703717217476Chembridge (Drug-like library)339.43143.52304
SGTC_2970909093841.86 μM0.36363617217104Chembridge (Drug-like library)339.43143.55304RNA processing & uracil transport