5570129

N-(2-ethylphenyl)pyrazine-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2154
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 673366
SMILES CCC1=CC=CC=C1NC(=O)C2=NC=CN=C2
Standardized SMILES CCc1ccccc1NC(=O)c2cnccn2
Molecular weight 227.2618
ALogP 1.68
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.11
% growth inhibition (Hom. pool) 5.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 673366
Download HIP data (tab-delimited text)  (excel)
Gene:CDC27(YBL084C)|FD-Score:3.81|P-value:7.04E-5|Clearance:0.21||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:DUT1(YBR252W)|FD-Score:4.33|P-value:7.48E-6|Clearance:0.52||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ENP2(YGR145W)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.18||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:PRP22(YER013W)|FD-Score:3.17|P-value:7.54E-4|Clearance:0.2||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RER2(YBR002C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.23||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:SUA7(YPR086W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.01||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:CDC27(YBL084C)|FD-Score:3.81|P-value:7.04E-5|Clearance:0.21||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:DUT1(YBR252W)|FD-Score:4.33|P-value:7.48E-6|Clearance:0.52||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ENP2(YGR145W)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.18||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:PRP22(YER013W)|FD-Score:3.17|P-value:7.54E-4|Clearance:0.2||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RER2(YBR002C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.23||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:SUA7(YPR086W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.01||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 673366
Download HOP data (tab-delimited text)  (excel)
Gene:ASG7(YJL170C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:AUA1(YFL010W-A)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BSP1(YPR171W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CAP2(YIL034C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:DIE2(YGR227W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ENV9(YOR246C)|FD-Score:10.3|P-value:4.63E-25||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FRA1(YLL029W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:HHT1(YBR010W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HMO1(YDR174W)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IME4(YGL192W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:LCB5(YLR260W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LDB7(YBL006C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LSB6(YJL100W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MAL11(YGR289C)|FD-Score:4.89|P-value:4.94E-7||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MAM1(YER106W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MON1(YGL124C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL17(YNL252C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH3(YCR092C)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MSS18(YPR134W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTC4(YBR255W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NFT1(YKR103W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NIP100(YPL174C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:PFA3(YNL326C)|FD-Score:4.31|P-value:8.32E-6||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PMT5(YDL093W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:RHR2(YIL053W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL12B(YDR418W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RSC2(YLR357W)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:SGN1(YIR001C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SIP5(YMR140W)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKG1(YKR100C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SKM1(YOL113W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SNO2(YNL334C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SPC72(YAL047C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPS18(YNL204C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRS2(YJL092W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SWS2(YNL081C)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:THO2(YNL139C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TIF1(YKR059W)|FD-Score:3.74|P-value:9.11E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TMT1(YER175C)|FD-Score:4.36|P-value:6.41E-6||SGD DESC:Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle Gene:TRP2(YER090W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUL1(YKL034W)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBC7(YMR022W)|FD-Score:4.32|P-value:7.68E-6||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VBA2(YBR293W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS30(YPL120W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS68(YOL129W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YCR050C(YCR050C_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR161W(YDR161W_p)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YGR093W(YGR093W_p)|FD-Score:4.4|P-value:5.48E-6||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR219W(YGR219W_d)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL026C(YHL026C_p)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YKR073C(YKR073C_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL032C(YLL032C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Putative protein of unknown function Gene:YML096W(YML096W_p)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YOP1(YPR028W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR200W(YOR200W_d)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPL264C(YPL264C_p)|FD-Score:-4.37|P-value:6.24E-6||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPR197C(YPR197C_d)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ASG7(YJL170C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:AUA1(YFL010W-A)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BSP1(YPR171W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CAP2(YIL034C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:DIE2(YGR227W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ENV9(YOR246C)|FD-Score:10.3|P-value:4.63E-25||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:FRA1(YLL029W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:HHT1(YBR010W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:HMO1(YDR174W)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IME4(YGL192W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:LCB5(YLR260W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LDB7(YBL006C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LSB6(YJL100W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MAL11(YGR289C)|FD-Score:4.89|P-value:4.94E-7||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MAM1(YER106W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MON1(YGL124C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL17(YNL252C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH3(YCR092C)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MSS18(YPR134W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTC4(YBR255W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NFT1(YKR103W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NIP100(YPL174C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:PFA3(YNL326C)|FD-Score:4.31|P-value:8.32E-6||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PMT5(YDL093W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:RHR2(YIL053W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL12B(YDR418W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RSC2(YLR357W)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:SGN1(YIR001C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SIP5(YMR140W)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKG1(YKR100C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SKM1(YOL113W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SNO2(YNL334C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SPC72(YAL047C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPS18(YNL204C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRS2(YJL092W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SWS2(YNL081C)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:THO2(YNL139C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TIF1(YKR059W)|FD-Score:3.74|P-value:9.11E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TMT1(YER175C)|FD-Score:4.36|P-value:6.41E-6||SGD DESC:Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle Gene:TRP2(YER090W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUL1(YKL034W)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBC7(YMR022W)|FD-Score:4.32|P-value:7.68E-6||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VBA2(YBR293W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS30(YPL120W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS68(YOL129W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YCR050C(YCR050C_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR161W(YDR161W_p)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YGR093W(YGR093W_p)|FD-Score:4.4|P-value:5.48E-6||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR219W(YGR219W_d)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL026C(YHL026C_p)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YKR073C(YKR073C_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL032C(YLL032C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Putative protein of unknown function Gene:YML096W(YML096W_p)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YOP1(YPR028W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR200W(YOR200W_d)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPL264C(YPL264C_p)|FD-Score:-4.37|P-value:6.24E-6||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPR197C(YPR197C_d)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR252W4.337.48E-60.52DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YBL084C3.817.04E-50.21CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YBR002C3.601.61E-40.23RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YPR086W3.373.75E-40.01SUA7Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
YGR145W3.363.96E-40.18ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YER013W3.177.54E-40.20PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YGR090W2.970.001470.16UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YLR229C2.820.002420.08CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YOR261C2.740.003110.02RPN8Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
YMR079W2.720.003270.05SEC14Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
YGL201C2.670.003810.08MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YDR302W2.590.004810.04GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YJL143W2.550.005350.01TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YER021W2.540.005547.87E-4RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YGR265W_d2.540.005550.01YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR246C10.304.63E-25ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YGR289C4.894.94E-7MAL11Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
YPR171W4.503.44E-6BSP1Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton
YGR093W_p4.405.48E-6YGR093W_pPutative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YER175C4.366.41E-6TMT1Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
YCR092C4.366.64E-6MSH3Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
YLR357W4.356.73E-6RSC2Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance
YMR022W4.327.68E-6UBC7Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
YNL326C4.318.32E-6PFA3Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
YLR260W4.211.30E-5LCB5Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules
YOL113W4.151.63E-5SKM1Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth
YKR103W4.141.70E-5NFT1Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds.
YHL026C_p4.141.72E-5YHL026C_pPutative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
YJL100W4.121.90E-5LSB6Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
YPR197C_d4.072.30E-5YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2154
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1936.09E-50SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.0857143
0.1432.27E-28SGTC_2413inermin 26.1 μMTimTec (Natural product derivative library)915100.0434783
0.1262.76E-22SGTC_29679089538 49.9 μMChembridge (Drug-like library)7282160.245902
0.1163.36E-19SGTC_3364111-0029 111.0 μMChemDiv (Drug-like library)31311750.142857
0.1119.29E-18SGTC_7334069-0007 56.1 μMChemDiv (Drug-like library)57174250.121212ERAD & cell cycle
0.0985.43E-14SGTC_1030844-0104 51.8 μMChemDiv (Drug-like library)53758040.218182ERAD & cell cycle
0.0903.70E-12SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.125ERAD & cell cycle
0.0871.83E-11SGTC_2231micatex 6.9 μMMiscellaneous207370.0576923
0.0871.85E-11SGTC_30909117696 49.5 μMChembridge (Drug-like library)79238210.469388
0.0832.05E-10SGTC_2710st078562 53.7 μMTimTec (Natural product derivative library)172509080.328358
0.0781.87E-9SGTC_1161k007-0610 110.0 μMChemDiv (Drug-like library)58438500.140625ERAD & cell cycle
0.0773.56E-9SGTC_21635671233 125.0 μMChembridge (Fragment library)7010230.0882353
0.0723.12E-8SGTC_33489149464 3.5 μMChembridge (Drug-like library)19514420.116883
0.0708.49E-8SGTC_1235benzylparaben 21.5 μMChemDiv (Drug-like library)71800.163636
0.0691.32E-7SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.0517241cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_22057238770200 μM0.642857279867Chembridge (Fragment library)278.104721.4913
SGTC_21175521686200 μM0.543478803312Chembridge (Fragment library)227.261821.71413
SGTC_21505556613200 μM0.510638743190Chembridge (Fragment library)213.235241.22813
SGTC_3215912994249.47 μM0.50909117682944Chembridge (Drug-like library)323.412083.36114
SGTC_3090911769649.47 μM0.4693887923821Chembridge (Drug-like library)297.2475534.37915
SGTC_21876125751200 μM0.458333874498Chembridge (Fragment library)235.1895861.15215RSC complex & mRNA processing
SGTC_3245913482149.47 μM0.44444417014901Chembridge (Drug-like library)307.349783.12224
SGTC_2885905773251.95 μM0.4339626471414Chembridge (Drug-like library)297.414564.71512
SGTC_21935925765200 μM0.365385877370Chembridge (Fragment library)241.28512.49622
SGTC_9861493-048384 μM0.34615440079ChemDiv (Drug-like library)256.256681.31914