5570423

1-[4-(4-fluorophenyl)piperazin-1-yl]-2-methylpropan-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2155
Screen concentration 76.0 μM
Source Chembridge (Fragment library)
PubChem CID 797402
SMILES CC(C)C(=O)N1CCN(CC1)C2=CC=C(C=C2)F
Standardized SMILES CC(C)C(=O)N1CCN(CC1)c2ccc(F)cc2
Molecular weight 250.3119
ALogP 2.54
H-bond donor count 0
H-bond acceptor count 3
Response signature ergosterol biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.28
% growth inhibition (Hom. pool) 5.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 797402
Download HIP data (tab-delimited text)  (excel)
Gene:ERG11(YHR007C)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.1||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:6.78|P-value:6.10E-12|Clearance:2.61||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:ERV1(YGR029W)|FD-Score:4.08|P-value:2.28E-5|Clearance:0.16||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:HSH49(YOR319W)|FD-Score:7.84|P-value:2.17E-15|Clearance:2.61||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:PRE6(YOL038W)|FD-Score:-3.75|P-value:8.83E-5|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP4(YPR178W)|FD-Score:3.31|P-value:4.68E-4|Clearance:0.07||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB8(YOR224C)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC17(YJL011C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.15||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPS3(YNL178W)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRN7(YJL025W)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SHQ1(YIL104C)|FD-Score:3.14|P-value:8.35E-4|Clearance:0.05||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SNM1(YDR478W)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.04||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:TEL2(YGR099W)|FD-Score:3.91|P-value:4.54E-5|Clearance:0.36||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIM10(YHR005C-A)|FD-Score:-3.44|P-value:2.90E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YDL163W(YDL163W_d)|FD-Score:4.16|P-value:1.57E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YDL196W(YDL196W_d)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ERG11(YHR007C)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.1||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:6.78|P-value:6.10E-12|Clearance:2.61||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:ERV1(YGR029W)|FD-Score:4.08|P-value:2.28E-5|Clearance:0.16||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:HSH49(YOR319W)|FD-Score:7.84|P-value:2.17E-15|Clearance:2.61||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:PRE6(YOL038W)|FD-Score:-3.75|P-value:8.83E-5|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP4(YPR178W)|FD-Score:3.31|P-value:4.68E-4|Clearance:0.07||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPB8(YOR224C)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC17(YJL011C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.15||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPS3(YNL178W)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRN7(YJL025W)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SHQ1(YIL104C)|FD-Score:3.14|P-value:8.35E-4|Clearance:0.05||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SNM1(YDR478W)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.04||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:TEL2(YGR099W)|FD-Score:3.91|P-value:4.54E-5|Clearance:0.36||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIM10(YHR005C-A)|FD-Score:-3.44|P-value:2.90E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YDL163W(YDL163W_d)|FD-Score:4.16|P-value:1.57E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YDL196W(YDL196W_d)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 797402
Download HOP data (tab-delimited text)  (excel)
Gene:ADD66(YKL206C)|FD-Score:-5.77|P-value:4.01E-9||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:AFT1(YGL071W)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC2(YCR082W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AKR1(YDR264C)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALG8(YOR067C)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ALT2(YDR111C_p)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:BCK1(YJL095W)|FD-Score:3.34|P-value:4.11E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CKB1(YGL019W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CLB4(YLR210W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:CWC21(YDR482C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DFG16(YOR030W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DFG5(YMR238W)|FD-Score:4.38|P-value:5.94E-6||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DUN1(YDL101C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EXG2(YDR261C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:HMG1(YML075C)|FD-Score:5.49|P-value:2.01E-8||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOL1(YNR055C)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:ILM1(YJR118C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:KAR5(YMR065W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:LSB3(YFR024C-A)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MSK1(YNL073W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:MSN4(YKL062W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MUP1(YGR055W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:MXR2(YCL033C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NQM1(YGR043C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:OPI3(YJR073C)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PCA1(YBR295W)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDC1(YLR044C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PHO86(YJL117W)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIL1(YGR086C)|FD-Score:4.55|P-value:2.75E-6||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:RGL1(YPL066W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.88|P-value:5.38E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:SEE1(YIL064W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SEY1(YOR165W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SMA2(YML066C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SPO20(YMR017W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:TDA11(YHR159W_p)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP4(YDR354W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:URM1(YIL008W)|FD-Score:5.27|P-value:6.73E-8||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VID22(YLR373C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS21(YOR089C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS61(YDR136C_d)|FD-Score:-5.39|P-value:3.49E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-5.14|P-value:1.34E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS8(YAL002W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YBR232C(YBR232C_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL119C(YDL119C_p)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR008C(YDR008C_d)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR111W(YGR111W_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YJR018W(YJR018W_d)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL027W(YKL027W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKR047W(YKR047W_d)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR232W(YLR232W_d)|FD-Score:-7.2|P-value:2.96E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Gene:YMR141C(YMR141C_d)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL195C(YNL195C_p)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL182C(YPL182C_d)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPL247C(YPL247C_p)|FD-Score:5.75|P-value:4.43E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPT6(YLR262C)|FD-Score:-4.01|P-value:2.98E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSR3(YKR053C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ADD66(YKL206C)|FD-Score:-5.77|P-value:4.01E-9||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:AFT1(YGL071W)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC2(YCR082W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AKR1(YDR264C)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALG8(YOR067C)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ALT2(YDR111C_p)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:BCK1(YJL095W)|FD-Score:3.34|P-value:4.11E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CKB1(YGL019W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CLB4(YLR210W)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:CWC21(YDR482C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DFG16(YOR030W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DFG5(YMR238W)|FD-Score:4.38|P-value:5.94E-6||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DUN1(YDL101C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EXG2(YDR261C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:HMG1(YML075C)|FD-Score:5.49|P-value:2.01E-8||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOL1(YNR055C)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:ILM1(YJR118C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:KAR5(YMR065W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:LSB3(YFR024C-A)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MSK1(YNL073W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:MSN4(YKL062W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MUP1(YGR055W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:MXR2(YCL033C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NQM1(YGR043C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:OPI3(YJR073C)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PCA1(YBR295W)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PDC1(YLR044C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PHO86(YJL117W)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIL1(YGR086C)|FD-Score:4.55|P-value:2.75E-6||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:RGL1(YPL066W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.88|P-value:5.38E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:SEE1(YIL064W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SEY1(YOR165W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SMA2(YML066C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SPO20(YMR017W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:TDA11(YHR159W_p)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP4(YDR354W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:URM1(YIL008W)|FD-Score:5.27|P-value:6.73E-8||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VID22(YLR373C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS21(YOR089C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS61(YDR136C_d)|FD-Score:-5.39|P-value:3.49E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-5.14|P-value:1.34E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS8(YAL002W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YBR232C(YBR232C_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL119C(YDL119C_p)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR008C(YDR008C_d)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR111W(YGR111W_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YJR018W(YJR018W_d)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL027W(YKL027W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YKR047W(YKR047W_d)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR232W(YLR232W_d)|FD-Score:-7.2|P-value:2.96E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Gene:YMR141C(YMR141C_d)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL195C(YNL195C_p)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL182C(YPL182C_d)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPL247C(YPL247C_p)|FD-Score:5.75|P-value:4.43E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPT6(YLR262C)|FD-Score:-4.01|P-value:2.98E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSR3(YKR053C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR319W7.842.17E-152.61HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YHR072W6.786.10E-122.61ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YDL163W_d4.161.57E-50.09YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
YGR029W4.082.28E-50.16ERV1Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
YGR099W3.914.54E-50.36TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YJL011C3.551.90E-40.14RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YDR478W3.413.26E-40.04SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YOR224C3.373.75E-40.06RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YPR178W3.314.68E-40.07PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YHR007C3.245.93E-40.10ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YIL104C3.148.35E-40.05SHQ1Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
YDL196W_d3.109.74E-40.15YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YPL007C2.950.001600.12TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YNL131W2.820.002380.02TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YDL103C2.800.002550.07QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL247C_p5.754.43E-9YPL247C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
YML075C5.492.01E-8HMG1HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
YIL008W5.276.73E-8URM1Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
YBR295W5.171.20E-7PCA1Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function
YGL019W4.731.10E-6CKB1Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
YNL195C_p4.631.81E-6YNL195C_pPutative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGR086C4.552.75E-6PIL1Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YMR238W4.385.94E-6DFG5Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
YNR055C4.082.24E-5HOL1Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake
YIL064W3.963.78E-5SEE1Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
YMR243C3.855.87E-5ZRC1Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication
YOR089C3.816.88E-5VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YDR264C3.778.05E-5AKR1Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication
YMR141C_d3.768.60E-5YMR141C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR043C3.541.97E-4NQM1Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift

GO enrichment analysis for SGTC_2155
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2282.24E-70SGTC_21475545932 89.7 μMChembridge (Fragment library)7974040.705882ergosterol biosynthesis
0.2251.28E-68SGTC_21856113692 200.0 μMChembridge (Fragment library)8741500.589744ergosterol biosynthesis
0.2121.04E-60SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0819672ergosterol biosynthesis
0.2021.95E-55SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.184211azole & statin
0.1756.11E-42SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.304348ergosterol biosynthesis
0.1675.05E-38SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride 61.8 μMTimTec (Natural product derivative library)170377580.16129ergosterol biosynthesis
0.1598.89E-35SGTC_2525harmine 47.9 μMMiscellaneous52809530.0677966ergosterol biosynthesis
0.1556.25E-33SGTC_2647atorvastatin 60.2 μMMiscellaneous608230.184211azole & statin
0.1547.54E-33SGTC_1097itavastatin 7.2 μMNIH Clinical Collection52824510.164557azole & statin
0.1533.89E-32SGTC_1638st008370 60.6 μMTimTec (Natural product derivative library)242071340.142857ergosterol biosynthesis
0.1503.98E-31SGTC_2753fluvastatin 17.1 μMMiscellaneous4461550.202899azole & statin
0.1491.79E-30SGTC_23779073351 62.6 μMChembridge (Fragment library)73379460.466667ergosterol biosynthesis
0.1344.47E-25SGTC_1547harmine 94.2 μMMiscellaneous52809530.0677966ergosterol biosynthesis
0.1292.54E-23SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.0689655ergosterol biosynthesis
0.1224.87E-21SGTC_5554296-0685 37.0 μMChemDiv (Drug-like library)29085400.084507ergosterol biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2147554593289.72 μM0.705882797404Chembridge (Fragment library)232.32142.33802ergosterol biosynthesis
SGTC_21856113692200 μM0.589744874150Chembridge (Fragment library)250.3118632.53703ergosterol biosynthesis
SGTC_3056909490049.47 μM0.46938825236784Chembridge (Drug-like library)342.4072233.88404
SGTC_1460k062-00599.15 μM0.4680851373085ChemDiv (Drug-like library)368.2328434.31513Golgi
SGTC_2377907335162.6 μM0.4666677337946Chembridge (Fragment library)281.78112.25613ergosterol biosynthesis
SGTC_23849036245200 μM0.4651166462766Chembridge (Fragment library)261.362621.83713
SGTC_16794130-12766.7 μM0.4313731005053Miscellaneous363.7707033.63705Golgi
SGTC_3219913111249.47 μM0.40425517612988Chembridge (Drug-like library)298.3546633.4513Golgi
SGTC_3259913848549.47 μM0.34693917646153Chembridge (Drug-like library)334.4083633.15315
SGTC_20204017018200 μM0.34146321127491Chembridge (Fragment library)320.36530.23957