5649887

N-ethyl-4-hydroxy-2-oxo-1H-quinoline-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2156
Screen concentration 115.0 μM
Source Chembridge (Fragment library)
PubChem CID 54680440
SMILES CCNC(=O)C1=C(C2=CC=CC=C2NC1=O)O
Standardized SMILES CCNC(=O)c1c(O)nc2ccccc2c1O
Molecular weight 232.2353
ALogP 0.23
H-bond donor count 3
H-bond acceptor count 3
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.41
% growth inhibition (Hom. pool) 14.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 54680440
Download HIP data (tab-delimited text)  (excel)
Gene:ASK1(YKL052C)|FD-Score:29.7|P-value:4.45E-194|Clearance:11.5||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress Gene:ESA1(YOR244W)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.13||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:EXO70(YJL085W)|FD-Score:5.68|P-value:6.62E-9|Clearance:0.55||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GCD11(YER025W)|FD-Score:5.13|P-value:1.42E-7|Clearance:0.17||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:9.39|P-value:3.09E-21|Clearance:3.15||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KAE1(YKR038C)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.02||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:MCM5(YLR274W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.08||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:NUP82(YJL061W)|FD-Score:4.33|P-value:7.30E-6|Clearance:0.08||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:POP4(YBR257W)|FD-Score:26.2|P-value:1.86E-151|Clearance:11.5||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP22(YER013W)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.22||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RAP1(YNL216W)|FD-Score:3.25|P-value:5.85E-4|Clearance:0.02||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RHO3(YIL118W)|FD-Score:-3.81|P-value:6.89E-5|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPA190(YOR341W)|FD-Score:4.01|P-value:3.00E-5|Clearance:0.26||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPC53(YDL150W)|FD-Score:6.24|P-value:2.18E-10|Clearance:0.56||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:14.7|P-value:3.72E-49|Clearance:2.62||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:4.97|P-value:3.39E-7|Clearance:0.23||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC12(YNR026C)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.11||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SEC31(YDL195W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SGV1(YPR161C)|FD-Score:-3.18|P-value:7.48E-4|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SPC98(YNL126W)|FD-Score:4.61|P-value:2.01E-6|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SRP101(YDR292C)|FD-Score:3.17|P-value:7.73E-4|Clearance:0.01||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:SRP72(YPL210C)|FD-Score:-4.7|P-value:1.28E-6|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:SUI2(YJR007W)|FD-Score:12.1|P-value:7.29E-34|Clearance:2.69||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:3.16|P-value:7.89E-4|Clearance:0.08||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF35(YDR429C)|FD-Score:3.4|P-value:3.34E-4|Clearance:0.03||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRM5(YHR070W)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.01||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:UTP15(YMR093W)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.1||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YCS4(YLR272C)|FD-Score:3.22|P-value:6.32E-4|Clearance:0.06||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL152W(YDL152W_d)|FD-Score:4.34|P-value:7.18E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR526C(YDR526C_d)|FD-Score:4.61|P-value:2.05E-6|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL238C(YPL238C_d)|FD-Score:4.74|P-value:1.08E-6|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ASK1(YKL052C)|FD-Score:29.7|P-value:4.45E-194|Clearance:11.5||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress Gene:ESA1(YOR244W)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.13||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:EXO70(YJL085W)|FD-Score:5.68|P-value:6.62E-9|Clearance:0.55||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GCD11(YER025W)|FD-Score:5.13|P-value:1.42E-7|Clearance:0.17||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:9.39|P-value:3.09E-21|Clearance:3.15||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KAE1(YKR038C)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.02||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:MCM5(YLR274W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.08||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:NUP82(YJL061W)|FD-Score:4.33|P-value:7.30E-6|Clearance:0.08||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:POP4(YBR257W)|FD-Score:26.2|P-value:1.86E-151|Clearance:11.5||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP22(YER013W)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.22||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RAP1(YNL216W)|FD-Score:3.25|P-value:5.85E-4|Clearance:0.02||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RHO3(YIL118W)|FD-Score:-3.81|P-value:6.89E-5|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPA190(YOR341W)|FD-Score:4.01|P-value:3.00E-5|Clearance:0.26||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPC53(YDL150W)|FD-Score:6.24|P-value:2.18E-10|Clearance:0.56||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:14.7|P-value:3.72E-49|Clearance:2.62||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:4.97|P-value:3.39E-7|Clearance:0.23||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC12(YNR026C)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.11||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SEC31(YDL195W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SGV1(YPR161C)|FD-Score:-3.18|P-value:7.48E-4|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SPC98(YNL126W)|FD-Score:4.61|P-value:2.01E-6|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SRP101(YDR292C)|FD-Score:3.17|P-value:7.73E-4|Clearance:0.01||SGD DESC:Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p Gene:SRP72(YPL210C)|FD-Score:-4.7|P-value:1.28E-6|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:SUI2(YJR007W)|FD-Score:12.1|P-value:7.29E-34|Clearance:2.69||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:3.16|P-value:7.89E-4|Clearance:0.08||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF35(YDR429C)|FD-Score:3.4|P-value:3.34E-4|Clearance:0.03||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRM5(YHR070W)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.01||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:UTP15(YMR093W)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.1||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YCS4(YLR272C)|FD-Score:3.22|P-value:6.32E-4|Clearance:0.06||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDL152W(YDL152W_d)|FD-Score:4.34|P-value:7.18E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDR526C(YDR526C_d)|FD-Score:4.61|P-value:2.05E-6|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL238C(YPL238C_d)|FD-Score:4.74|P-value:1.08E-6|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 54680440
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AMN1(YBR158W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ANP1(YEL036C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APM1(YPL259C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARP8(YOR141C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ASN2(YGR124W)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CLD1(YGR110W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COG7(YGL005C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK3(YML112W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTL1(YMR180C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:DIA4(YHR011W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:ERV46(YAL042W)|FD-Score:4.88|P-value:5.40E-7||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FMP43(YGR243W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GEA1(YJR031C)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:HTA1(YDR225W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IME4(YGL192W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:LHS1(YKL073W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LIA1(YJR070C)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LYS14(YDR034C)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MBP1(YDL056W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MRPS12(YNR036C)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MTQ2(YDR140W)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NAM8(YHR086W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NHX1(YDR456W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NPT1(YOR209C)|FD-Score:3.73|P-value:9.44E-5||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NUT1(YGL151W)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:PET191(YJR034W)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PIP2(YOR363C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PUG1(YER185W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RAD27(YKL113C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RBS1(YDL189W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RHR2(YIL053W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIM4(YHL024W)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPL20B(YOR312C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:SIN4(YNL236W)|FD-Score:8.65|P-value:2.65E-18||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SMI1(YGR229C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPC72(YAL047C)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SRO9(YCL037C)|FD-Score:3.8|P-value:7.09E-5||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSO1(YPL232W)|FD-Score:4.33|P-value:7.57E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SWA2(YDR320C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SXM1(YDR395W)|FD-Score:4.42|P-value:4.89E-6||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TOF1(YNL273W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TUM1(YOR251C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UBP12(YJL197W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:UBP15(YMR304W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:VID30(YGL227W)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VMA16(YHR026W)|FD-Score:-4.35|P-value:6.66E-6||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS9(YML097C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBL071C(YBL071C_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YGR122W(YGR122W)|FD-Score:-5.39|P-value:3.50E-8||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YKL053W(YKL053W_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YML108W(YML108W_p)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YML122C(YML122C_d)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL226W(YNL226W_d)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNL235C(YNL235C_d)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNR029C(YNR029C_p)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:ACO1(YLR304C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AMN1(YBR158W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ANP1(YEL036C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APM1(YPL259C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARP8(YOR141C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ASN2(YGR124W)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CLD1(YGR110W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COG7(YGL005C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK3(YML112W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTL1(YMR180C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:DIA4(YHR011W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:ERV46(YAL042W)|FD-Score:4.88|P-value:5.40E-7||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FMP43(YGR243W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GEA1(YJR031C)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:HTA1(YDR225W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IME4(YGL192W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:LHS1(YKL073W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LIA1(YJR070C)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LYS14(YDR034C)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MBP1(YDL056W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MRPS12(YNR036C)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MTQ2(YDR140W)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NAM8(YHR086W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NHX1(YDR456W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NPT1(YOR209C)|FD-Score:3.73|P-value:9.44E-5||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NUT1(YGL151W)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:PET191(YJR034W)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PIP2(YOR363C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PUG1(YER185W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RAD27(YKL113C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RBS1(YDL189W)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RHR2(YIL053W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIM4(YHL024W)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPL20B(YOR312C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:SIN4(YNL236W)|FD-Score:8.65|P-value:2.65E-18||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SMI1(YGR229C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPC72(YAL047C)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SRO9(YCL037C)|FD-Score:3.8|P-value:7.09E-5||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSO1(YPL232W)|FD-Score:4.33|P-value:7.57E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SWA2(YDR320C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SXM1(YDR395W)|FD-Score:4.42|P-value:4.89E-6||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TOF1(YNL273W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TUM1(YOR251C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UBP12(YJL197W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:UBP15(YMR304W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:VID30(YGL227W)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VMA16(YHR026W)|FD-Score:-4.35|P-value:6.66E-6||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS9(YML097C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBL071C(YBL071C_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YGR122W(YGR122W)|FD-Score:-5.39|P-value:3.50E-8||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YKL053W(YKL053W_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YML108W(YML108W_p)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YML122C(YML122C_d)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL226W(YNL226W_d)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNL235C(YNL235C_d)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNR029C(YNR029C_p)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL052C29.704.45E-19411.50ASK1Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
YBR257W26.201.86E-15111.50POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YHR062C14.703.72E-492.62RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YJR007W12.107.29E-342.69SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YER133W9.393.09E-213.15GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDL150W6.242.18E-100.56RPC53RNA polymerase III subunit C53
YJL085W5.686.62E-90.55EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YER025W5.131.42E-70.17GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YHR069C4.973.39E-70.23RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YPL238C_d4.741.08E-60.13YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YNL126W4.612.01E-60.00SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YDR526C_d4.612.05E-60.27YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL152W_d4.347.18E-60.00YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YJL061W4.337.30E-60.08NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
YER013W4.251.05E-50.22PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL236W8.652.65E-18SIN4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
YAL042W4.885.40E-7ERV46Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport
YAL047C4.691.39E-6SPC72Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization
YGL227W4.542.76E-6VID30Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm
YDR395W4.424.89E-6SXM1Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
YPL232W4.337.57E-6SSO1Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
YKL053W_d4.231.17E-5YKL053W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1
YJR034W4.151.68E-5PET191Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery
YHR011W4.052.58E-5DIA4Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth
YOR251C3.963.75E-5TUM1Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized
YGL151W3.875.54E-5NUT1Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription
YER185W3.865.75E-5PUG1Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins
YNL235C_d3.846.27E-5YNL235C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
YCL037C3.807.09E-5SRO9Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
YML122C_d3.807.14E-5YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2156
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4850SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)23847490NARPP1 & pyrimidine depletion
0.4408.08E-278SGTC_20575236571 38.3 μMChembridge (Fragment library)54684039NARPP1 & pyrimidine depletion
0.4084.64E-235SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)5098884NARPP1 & pyrimidine depletion
0.3541.03E-173SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)6683NAcopper-dependent oxidative stress
0.2714.32E-100SGTC_1959st077091 55.8 μMTimTec (Natural product derivative library)821356NARPP1 & pyrimidine depletion
0.2701.16E-98SGTC_1766st045414 44.8 μMTimTec (Natural product derivative library)253960NARPP1 & pyrimidine depletion
0.2358.69E-75SGTC_27915663188 71.4 μMChembridge (Drug-like library)54690634NARPP1 & pyrimidine depletion
0.2221.21E-66SGTC_10164226-1401 38.0 μMChemDiv (Drug-like library)2901613NARNA pol III & RNase P/MRP
0.2131.84E-61SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)46499474NARNA pol III & RNase P/MRP
0.2121.04E-60SGTC_1872amphotericin b 148.0 nMMicrosource (Natural product library)16667679NARNA pol III & RNase P/MRP
0.1944.29E-51SGTC_2023594-0513 34.8 μMChemDiv (Drug-like library)708429NAPDR1
0.1804.85E-44SGTC_2686alizarin 95.4 μMMiscellaneous6293NARPP1 & pyrimidine depletion
0.1619.78E-36SGTC_6671498-1044 59.0 μMChemDiv (Drug-like library)6739982NARPP1 & pyrimidine depletion
0.1611.04E-35SGTC_32329132874 49.5 μMChembridge (Drug-like library)3784940NARPP1 & pyrimidine depletion
0.1441.02E-28SGTC_15346-azauridine 81.6 μMTimTec (Pure natural product library)233502NARNA pol III & RNase P/MRP

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature