5657123

2-(3,4-dimethylphenyl)-3a,4,7,7a-tetrahydro-octahydro-1H-4,7-epoxyisoindole-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2158
Screen concentration 3.6 μM
Source Chembridge (Fragment library)
PubChem CID 2858827
SMILES CC1=C(C=C(C=C1)N2C(=O)C3C4C=CC(C3C2=O)O4)C
Standardized SMILES Cc1ccc(cc1C)N2C(=O)C3C4OC(C=C4)C3C2=O
Molecular weight 269.2952
ALogP 1.82
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.28
% growth inhibition (Hom. pool) 8.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2858827
Download HIP data (tab-delimited text)  (excel)
Gene:ALG2(YGL065C)|FD-Score:-3.92|P-value:4.41E-5|Clearance:0||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ALG7(YBR243C)|FD-Score:5.34|P-value:4.54E-8|Clearance:0.1||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:ARC35(YNR035C)|FD-Score:4.4|P-value:5.30E-6|Clearance:0.59||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ARH1(YDR376W)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.02||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:CBF2(YGR140W)|FD-Score:6.49|P-value:4.24E-11|Clearance:0.26||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC15(YAR019C)|FD-Score:8.9|P-value:2.68E-19|Clearance:2.41||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CWC25(YNL245C)|FD-Score:5.74|P-value:4.65E-9|Clearance:0.4||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DED1(YOR204W)|FD-Score:6.23|P-value:2.37E-10|Clearance:0.17||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:KRR1(YCL059C)|FD-Score:5.24|P-value:7.87E-8|Clearance:0.52||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:LTO1(YNL260C_p)|FD-Score:3.76|P-value:8.66E-5|Clearance:0.01||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:MED11(YMR112C)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.21||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:MTR3(YGR158C)|FD-Score:6.06|P-value:6.96E-10|Clearance:0.31||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOP53(YPL146C)|FD-Score:3.09|P-value:9.90E-4|Clearance:0.27||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PIS1(YPR113W)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.03||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PRE2(YPR103W)|FD-Score:4.62|P-value:1.96E-6|Clearance:0.03||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PZF1(YPR186C)|FD-Score:3.1|P-value:9.54E-4|Clearance:0.01||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RLI1(YDR091C)|FD-Score:-4.59|P-value:2.25E-6|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPN6(YDL097C)|FD-Score:4.72|P-value:1.17E-6|Clearance:0.07||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPN8(YOR261C)|FD-Score:3.14|P-value:8.40E-4|Clearance:0.04||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPS13(YDR064W)|FD-Score:4.58|P-value:2.31E-6|Clearance:0.18||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS31(YLR167W)|FD-Score:3.82|P-value:6.74E-5|Clearance:0.06||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SEC24(YIL109C)|FD-Score:4.65|P-value:1.63E-6|Clearance:0.04||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SRB4(YER022W)|FD-Score:3.63|P-value:1.39E-4|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:STS1(YIR011C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.15||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:SUI3(YPL237W)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.05||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:UTR5(YEL035C_p)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.04||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YSH1(YLR277C)|FD-Score:3.75|P-value:8.83E-5|Clearance:0.08||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:ALG2(YGL065C)|FD-Score:-3.92|P-value:4.41E-5|Clearance:0||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ALG7(YBR243C)|FD-Score:5.34|P-value:4.54E-8|Clearance:0.1||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:ARC35(YNR035C)|FD-Score:4.4|P-value:5.30E-6|Clearance:0.59||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:ARH1(YDR376W)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.02||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:CBF2(YGR140W)|FD-Score:6.49|P-value:4.24E-11|Clearance:0.26||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC15(YAR019C)|FD-Score:8.9|P-value:2.68E-19|Clearance:2.41||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CWC25(YNL245C)|FD-Score:5.74|P-value:4.65E-9|Clearance:0.4||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DED1(YOR204W)|FD-Score:6.23|P-value:2.37E-10|Clearance:0.17||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:KRR1(YCL059C)|FD-Score:5.24|P-value:7.87E-8|Clearance:0.52||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:LTO1(YNL260C_p)|FD-Score:3.76|P-value:8.66E-5|Clearance:0.01||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:MED11(YMR112C)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.21||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:MTR3(YGR158C)|FD-Score:6.06|P-value:6.96E-10|Clearance:0.31||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOP53(YPL146C)|FD-Score:3.09|P-value:9.90E-4|Clearance:0.27||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PIS1(YPR113W)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.03||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PRE2(YPR103W)|FD-Score:4.62|P-value:1.96E-6|Clearance:0.03||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PZF1(YPR186C)|FD-Score:3.1|P-value:9.54E-4|Clearance:0.01||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RLI1(YDR091C)|FD-Score:-4.59|P-value:2.25E-6|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPN6(YDL097C)|FD-Score:4.72|P-value:1.17E-6|Clearance:0.07||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPN8(YOR261C)|FD-Score:3.14|P-value:8.40E-4|Clearance:0.04||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPS13(YDR064W)|FD-Score:4.58|P-value:2.31E-6|Clearance:0.18||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS31(YLR167W)|FD-Score:3.82|P-value:6.74E-5|Clearance:0.06||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:SEC24(YIL109C)|FD-Score:4.65|P-value:1.63E-6|Clearance:0.04||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SRB4(YER022W)|FD-Score:3.63|P-value:1.39E-4|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:STS1(YIR011C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.15||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:SUI3(YPL237W)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.05||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:UTR5(YEL035C_p)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.04||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YSH1(YLR277C)|FD-Score:3.75|P-value:8.83E-5|Clearance:0.08||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2858827
Download HOP data (tab-delimited text)  (excel)
Gene:AHT1(YHR093W_d)|FD-Score:4.95|P-value:3.78E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:AIM44(YPL158C)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALY2(YJL084C)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ARP6(YLR085C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BEM1(YBR200W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:CDH1(YGL003C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CIN1(YOR349W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CTR1(YPR124W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:HFA1(YMR207C)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:MOS2(YGR235C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MRP10(YDL045W-A)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:PAC2(YER007W)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PET20(YPL159C)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:RFS1(YBR052C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:SLH1(YGR271W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:STR3(YGL184C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:SWR1(YDR334W)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SXM1(YDR395W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TRP1(YDR007W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TSC3(YBR058C-A)|FD-Score:9.25|P-value:1.08E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUM1(YOR251C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:URA4(YLR420W)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VPS71(YML041C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YDL241W(YDL241W_p)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR269C(YDR269C_d)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR219W(YGR219W_d)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL026C(YHL026C_p)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YHR095W(YHR095W_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR182W-A(YMR182W-A_p)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Putative protein of unknown function Gene:YRR1(YOR162C)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZWF1(YNL241C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AHT1(YHR093W_d)|FD-Score:4.95|P-value:3.78E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:AIM44(YPL158C)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALY2(YJL084C)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ARP6(YLR085C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BEM1(YBR200W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:CDH1(YGL003C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CIN1(YOR349W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CTR1(YPR124W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:HFA1(YMR207C)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:MOS2(YGR235C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MRP10(YDL045W-A)|FD-Score:-4.05|P-value:2.56E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:PAC2(YER007W)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PET20(YPL159C)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:RFS1(YBR052C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:SLH1(YGR271W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:STR3(YGL184C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:SWR1(YDR334W)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SXM1(YDR395W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:TRP1(YDR007W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TSC3(YBR058C-A)|FD-Score:9.25|P-value:1.08E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUM1(YOR251C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:URA4(YLR420W)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VPS71(YML041C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YDL241W(YDL241W_p)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR269C(YDR269C_d)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR219W(YGR219W_d)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL026C(YHL026C_p)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YHR095W(YHR095W_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR182W-A(YMR182W-A_p)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Putative protein of unknown function Gene:YRR1(YOR162C)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZWF1(YNL241C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YAR019C8.902.68E-192.41CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
YGR140W6.494.24E-110.26CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YOR204W6.232.37E-100.17DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YGR158C6.066.96E-100.31MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YNL245C5.744.65E-90.40CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YBR243C5.344.54E-80.10ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YCL059C5.247.87E-80.52KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
YDL097C4.721.17E-60.07RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YIL109C4.651.63E-60.04SEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
YPR103W4.621.96E-60.03PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YDR064W4.582.31E-60.18RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YNR035C4.405.30E-60.59ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YLR167W3.826.74E-50.06RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YNL260C_p3.768.66E-50.01LTO1_pEssential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YLR277C3.758.83E-50.08YSH1Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A9.251.08E-20TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YHR093W_d4.953.78E-7AHT1_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region
YER007W4.602.08E-6PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YLR085C4.141.70E-5ARP6Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YPL159C4.022.94E-5PET20Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome
YDR007W3.953.98E-5TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YMR207C3.894.94E-5HFA1Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
YDR395W3.875.53E-5SXM1Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
YDR334W3.778.29E-5SWR1Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YJL084C3.739.74E-5ALY2Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication
YHR095W_d3.551.92E-4YHR095W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL184C3.433.03E-4STR3Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p
YGL003C3.403.37E-4CDH1Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p
YML041C3.275.36E-4VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YNL241C3.265.52E-4ZWF1Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2158
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2542.34E-87SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.267857tubulin folding & SWR complex
0.2369.33E-76SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.161765TSC3-RPN4
0.2361.26E-75SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.149254
0.2326.32E-73SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0508475TSC3-RPN4
0.2277.54E-70SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0862069
0.2253.11E-68SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.176471
0.2244.17E-68SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.125TSC3-RPN4
0.2125.42E-61SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0806452TSC3-RPN4
0.2126.69E-61SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0847458TSC3-RPN4
0.2081.78E-58SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0666667TSC3-RPN4
0.2072.44E-58SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.153846TSC3-RPN4
0.2074.14E-58SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.578947tubulin folding & SWR complex
0.2069.10E-58SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.108108TSC3-RPN4
0.2061.02E-57SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.111111TSC3-RPN4
0.2033.80E-56SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.125TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_14863970-07934.53 μM0.5945952844330ChemDiv (Drug-like library)320.13811.59203
SGTC_2103537813810 μM0.583333244142Chembridge (Fragment library)241.242040.84403
SGTC_2200660060421.91 μM0.5789472904777Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_14873970-07952.82 μM0.5753765103ChemDiv (Drug-like library)320.13811.59203DNA intercalators
SGTC_1903970-07903.72 μM0.5365852832352ChemDiv (Drug-like library)286.23960.73805
SGTC_21735763948167 μM0.5365852867177Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_9963970-07991.8 μM0.5227276615648ChemDiv (Drug-like library)299.321181.704
SGTC_216156625048.51 μM0.4523812859334Chembridge (Fragment library)275.68711.50803tubulin folding & SWR complex
SGTC_21405586273200 μM0.348837854421Chembridge (Fragment library)223.65562.1402RSC complex & mRNA processing
SGTC_20985376439882.5 nM0.342844116Chembridge (Fragment library)255.268621.04903