5660466

4-(5-methylfuran-2-yl)aniline hydrochloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2160
Screen concentration 132.9 μM
Source Chembridge (Fragment library)
PubChem CID 2859134
SMILES CC1=CC=C(O1)C2=CC=C(C=C2)N.Cl
Standardized SMILES Cc1oc(cc1)c2ccc(N)cc2
Molecular weight 209.6721
ALogP 2.52
H-bond donor count 2
H-bond acceptor count 2
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.6
% growth inhibition (Hom. pool) 4.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2859134
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:6|P-value:9.88E-10|Clearance:0.4||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC15(YAR019C)|FD-Score:3.96|P-value:3.69E-5|Clearance:0.15||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:DCP2(YNL118C)|FD-Score:3.37|P-value:3.76E-4|Clearance:0.07||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:DPM1(YPR183W)|FD-Score:4.38|P-value:5.98E-6|Clearance:0.06||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:EFB1(YAL003W)|FD-Score:3.3|P-value:4.92E-4|Clearance:0.04||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERG8(YMR220W)|FD-Score:4.3|P-value:8.73E-6|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FIP1(YJR093C)|FD-Score:5.6|P-value:1.07E-8|Clearance:0.15||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GCD10(YNL062C)|FD-Score:4.22|P-value:1.22E-5|Clearance:0.26||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:IRR1(YIL026C)|FD-Score:4.32|P-value:7.83E-6|Clearance:0.02||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LCP5(YER127W)|FD-Score:-3.12|P-value:9.12E-4|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:LSG1(YGL099W)|FD-Score:7.09|P-value:6.69E-13|Clearance:0.91||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MSS4(YDR208W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.03||SGD DESC:Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation Gene:NFS1(YCL017C)|FD-Score:-3.94|P-value:4.05E-5|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NMD3(YHR170W)|FD-Score:5.1|P-value:1.68E-7|Clearance:0.47||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP56(YLR197W)|FD-Score:4.49|P-value:3.58E-6|Clearance:0.11||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NSP1(YJL041W)|FD-Score:8.24|P-value:8.92E-17|Clearance:1.15||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PSF1(YDR013W)|FD-Score:3.64|P-value:1.39E-4|Clearance:0.03||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RHO1(YPR165W)|FD-Score:5.45|P-value:2.50E-8|Clearance:0.35||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPF2(YKR081C)|FD-Score:4.29|P-value:8.91E-6|Clearance:0.07||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL10(YLR075W)|FD-Score:-3.94|P-value:4.05E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:SEN54(YPL083C)|FD-Score:3.79|P-value:7.50E-5|Clearance:0.16||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SHQ1(YIL104C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.02||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SMX3(YPR182W)|FD-Score:3.23|P-value:6.16E-4|Clearance:0.17||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:TIM23(YNR017W)|FD-Score:3.82|P-value:6.76E-5|Clearance:0.03||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TRE2(YOR256C)|FD-Score:3.58|P-value:1.75E-4|Clearance:0.01||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YEF3(YLR249W)|FD-Score:-3.19|P-value:7.16E-4|Clearance:0||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YLR198C(YLR198C_d)|FD-Score:-3.65|P-value:1.32E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YML6(YML025C)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.14||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YNL150W(YNL150W_d)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPR142C(YPR142C_d)|FD-Score:6.18|P-value:3.18E-10|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:ARP2(YDL029W)|FD-Score:6|P-value:9.88E-10|Clearance:0.4||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC15(YAR019C)|FD-Score:3.96|P-value:3.69E-5|Clearance:0.15||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:DCP2(YNL118C)|FD-Score:3.37|P-value:3.76E-4|Clearance:0.07||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:DPM1(YPR183W)|FD-Score:4.38|P-value:5.98E-6|Clearance:0.06||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:EFB1(YAL003W)|FD-Score:3.3|P-value:4.92E-4|Clearance:0.04||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERG8(YMR220W)|FD-Score:4.3|P-value:8.73E-6|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FIP1(YJR093C)|FD-Score:5.6|P-value:1.07E-8|Clearance:0.15||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GCD10(YNL062C)|FD-Score:4.22|P-value:1.22E-5|Clearance:0.26||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:IRR1(YIL026C)|FD-Score:4.32|P-value:7.83E-6|Clearance:0.02||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LCP5(YER127W)|FD-Score:-3.12|P-value:9.12E-4|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:LSG1(YGL099W)|FD-Score:7.09|P-value:6.69E-13|Clearance:0.91||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MSS4(YDR208W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.03||SGD DESC:Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation Gene:NFS1(YCL017C)|FD-Score:-3.94|P-value:4.05E-5|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NMD3(YHR170W)|FD-Score:5.1|P-value:1.68E-7|Clearance:0.47||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP56(YLR197W)|FD-Score:4.49|P-value:3.58E-6|Clearance:0.11||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NSP1(YJL041W)|FD-Score:8.24|P-value:8.92E-17|Clearance:1.15||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PSF1(YDR013W)|FD-Score:3.64|P-value:1.39E-4|Clearance:0.03||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RHO1(YPR165W)|FD-Score:5.45|P-value:2.50E-8|Clearance:0.35||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPF2(YKR081C)|FD-Score:4.29|P-value:8.91E-6|Clearance:0.07||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL10(YLR075W)|FD-Score:-3.94|P-value:4.05E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:SEN54(YPL083C)|FD-Score:3.79|P-value:7.50E-5|Clearance:0.16||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SHQ1(YIL104C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.02||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SMX3(YPR182W)|FD-Score:3.23|P-value:6.16E-4|Clearance:0.17||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:TIM23(YNR017W)|FD-Score:3.82|P-value:6.76E-5|Clearance:0.03||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TRE2(YOR256C)|FD-Score:3.58|P-value:1.75E-4|Clearance:0.01||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YEF3(YLR249W)|FD-Score:-3.19|P-value:7.16E-4|Clearance:0||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YLR198C(YLR198C_d)|FD-Score:-3.65|P-value:1.32E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YML6(YML025C)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.14||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YNL150W(YNL150W_d)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPR142C(YPR142C_d)|FD-Score:6.18|P-value:3.18E-10|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2859134
Download HOP data (tab-delimited text)  (excel)
Gene:AFG3(YER017C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ASE1(YOR058C)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:BRP1(YGL007W_d)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:COG6(YNL041C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIA1(YMR316W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ECM34(YHL043W)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:FAR3(YMR052W)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:GAL11(YOL051W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GBP2(YCL011C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GDT1(YBR187W_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GID7(YCL039W)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:HIS5(YIL116W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HMF1(YER057C)|FD-Score:5.41|P-value:3.13E-8||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HSP31(YDR533C)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:LSM1(YJL124C)|FD-Score:-4.67|P-value:1.51E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MDS3(YGL197W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MRPS12(YNR036C)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:OCA5(YHL029C_p)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OSH3(YHR073W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAI3(YMR174C)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PAU13(YHL046C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PHO88(YBR106W)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMS1(YNL082W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:POF1(YCL047C)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PTH2(YBL057C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PYC2(YBR218C)|FD-Score:4.47|P-value:3.95E-6||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:REV3(YPL167C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RFS1(YBR052C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RNH203(YLR154C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPN4(YDL020C)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:-5.07|P-value:2.03E-7||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:SAT4(YCR008W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SCW10(YMR305C)|FD-Score:5.26|P-value:7.32E-8||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SER1(YOR184W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SGF11(YPL047W)|FD-Score:4.92|P-value:4.33E-7||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SKI2(YLR398C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SSA3(YBL075C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STE23(YLR389C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:SUR4(YLR372W)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TGL4(YKR089C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:TIM21(YGR033C)|FD-Score:4.28|P-value:9.44E-6||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TRP1(YDR007W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBR1(YGR184C)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:UPF3(YGR072W)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VAM6(YDL077C)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMS1(YDR049W)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:XRS2(YDR369C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YAR047C(YAR047C_d)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR134W(YBR134W_d)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL071W(YKL071W_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR040C(YLR040C_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR446W(YLR446W_p)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YMR1(YJR110W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YNL338W(YNL338W_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOL047C(YOL047C_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR345C(YOR345C_d)|FD-Score:4.17|P-value:1.56E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPL088W(YPL088W_p)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YPR063C(YPR063C_p)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:ER-localized protein of unknown function Gene:YRA2(YKL214C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:YRR1(YOR162C)|FD-Score:-4.1|P-value:2.09E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:AFG3(YER017C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ASE1(YOR058C)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:BRP1(YGL007W_d)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:COG6(YNL041C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DIA1(YMR316W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ECM34(YHL043W)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:FAR3(YMR052W)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:GAL11(YOL051W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GBP2(YCL011C)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GDT1(YBR187W_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GID7(YCL039W)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:HIS5(YIL116W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HMF1(YER057C)|FD-Score:5.41|P-value:3.13E-8||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HSP31(YDR533C)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:LSM1(YJL124C)|FD-Score:-4.67|P-value:1.51E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MDS3(YGL197W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MRPS12(YNR036C)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:OCA5(YHL029C_p)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OSH3(YHR073W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAI3(YMR174C)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:Cytoplasmic proteinase A (Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact Gene:PAU13(YHL046C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PHO88(YBR106W)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMS1(YNL082W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:POF1(YCL047C)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PTH2(YBL057C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PYC2(YBR218C)|FD-Score:4.47|P-value:3.95E-6||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:REV3(YPL167C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RFS1(YBR052C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RNH203(YLR154C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPN4(YDL020C)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:-5.07|P-value:2.03E-7||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:SAT4(YCR008W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SCW10(YMR305C)|FD-Score:5.26|P-value:7.32E-8||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SER1(YOR184W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SGF11(YPL047W)|FD-Score:4.92|P-value:4.33E-7||SGD DESC:Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation Gene:SKI2(YLR398C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SSA3(YBL075C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STE23(YLR389C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:SUR4(YLR372W)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TGL4(YKR089C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:TIM21(YGR033C)|FD-Score:4.28|P-value:9.44E-6||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TRP1(YDR007W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBR1(YGR184C)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:UPF3(YGR072W)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VAM6(YDL077C)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMS1(YDR049W)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:XRS2(YDR369C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YAR047C(YAR047C_d)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR134W(YBR134W_d)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL071W(YKL071W_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR040C(YLR040C_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR446W(YLR446W_p)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YMR1(YJR110W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YNL338W(YNL338W_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOL047C(YOL047C_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR345C(YOR345C_d)|FD-Score:4.17|P-value:1.56E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPL088W(YPL088W_p)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YPR063C(YPR063C_p)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:ER-localized protein of unknown function Gene:YRA2(YKL214C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:YRR1(YOR162C)|FD-Score:-4.1|P-value:2.09E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL041W8.248.92E-171.15NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YGL099W7.096.69E-130.91LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YPR142C_d6.183.18E-100.18YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YDL029W6.009.88E-100.40ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YJR093C5.601.07E-80.15FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YPR165W5.452.50E-80.35RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YHR170W5.101.68E-70.47NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YML025C4.631.85E-60.14YML6Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
YLR197W4.493.58E-60.11NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YPR183W4.385.98E-60.06DPM1Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation
YIL026C4.327.83E-60.02IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YMR220W4.308.73E-60.00ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YKR081C4.298.91E-60.07RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YNL062C4.221.22E-50.26GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YAR019C3.963.69E-50.15CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER057C5.413.13E-8HMF1Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro
YMR305C5.267.32E-8SCW10Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p
YLR446W_p5.131.45E-7YLR446W_pPutative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene
YPL047W4.924.33E-7SGF11Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
YGR072W4.602.08E-6UPF3Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance
YDL077C4.513.25E-6VAM6Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
YBR218C4.473.95E-6PYC2Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
YDL020C4.385.86E-6RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YGR033C4.289.44E-6TIM21Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity
YKL071W_p4.211.27E-5YKL071W_pPutative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YOR345C_d4.171.56E-5YOR345C_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YDR369C4.052.57E-5XRS2Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
YBL057C4.022.91E-5PTH2One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth
YLR389C3.953.83E-5STE23Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family
YMR052W3.953.89E-5FAR3Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2160
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1476.58E-30SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.071428660S ribosome export
0.1271.60E-22SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.067796660S ribosome export
0.1263.47E-22SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.081081160S ribosome export
0.1195.38E-20SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.074074160S ribosome export
0.1186.60E-20SGTC_32519135047 49.5 μMChembridge (Drug-like library)176829630.095238160S ribosome export
0.1187.62E-20SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.10769260S ribosome export
0.1181.13E-19SGTC_15101123-0200 71.5 μMChemDiv (Drug-like library)53788120.0833333
0.1142.25E-18SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.096774260S ribosome export
0.1126.07E-18SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.084745860S ribosome export
0.1056.99E-16SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.078431460S ribosome export
0.1032.24E-15SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.11538560S ribosome export
0.1032.35E-15SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.10344860S ribosome export
0.1025.38E-15SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.069444460S ribosome export
0.0952.21E-13SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.160S ribosome export
0.0953.54E-13SGTC_1833914-0051 30.6 μMChemDiv (Drug-like library)15962320.04687560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_22987976326193.81 μM0.3252235818Chembridge (Fragment library)203.237121.95623
SGTC_930109-004559.23 μM0.32432488733ChemDiv (Drug-like library)210.23132.53413DNA damage response
SGTC_12330325-040814.2 μM0.32258112874ChemDiv (Drug-like library)211.262423.93813
SGTC_2084530607860.64 μM0.292683679340Chembridge (Fragment library)258.2990.79326
SGTC_21945927483104.55 μM0.275760979Chembridge (Fragment library)201.221241.89112
SGTC_577r071-000338.6 μM0.270274568517ChemDiv (Drug-like library)276.332443.41523fatty acid desaturase (OLE1)
SGTC_2236dapsone200 μM0.2666672955Miscellaneous248.300881.43924
SGTC_21525568786200 μM0.2619055399883Chembridge (Fragment library)226.23071.76424
SGTC_22997976368114.88 μM0.2619052979718Chembridge (Fragment library)223.65562.13423
SGTC_23399000433200 μM0.2619052988954Chembridge (Fragment library)203.237121.95623