5662504

2-(2-chlorophenyl)-3a,4,7,7a-tetrahydro-octahydro-1H-4,7-epoxyisoindole-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2161
Screen concentration 8.5 μM
Source Chembridge (Fragment library)
PubChem CID 2859334
SMILES C1=CC=C(C(=C1)N2C(=O)C3C4C=CC(C3C2=O)O4)Cl
Standardized SMILES Clc1ccccc1N2C(=O)C3C4OC(C=C4)C3C2=O
Molecular weight 275.6871
ALogP 1.51
H-bond donor count 0
H-bond acceptor count 3
Response signature tubulin folding & SWR complex

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.62
% growth inhibition (Hom. pool) 6.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2859334
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:-3.76|P-value:8.39E-5|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BUR6(YER159C)|FD-Score:-7.35|P-value:1.01E-13|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CDC11(YJR076C)|FD-Score:-3.73|P-value:9.50E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC42(YLR229C)|FD-Score:-3.77|P-value:8.09E-5|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:CDC60(YPL160W)|FD-Score:-4.59|P-value:2.21E-6|Clearance:0||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:ERG11(YHR007C)|FD-Score:4.32|P-value:7.67E-6|Clearance:0.29||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MET30(YIL046W)|FD-Score:-4.54|P-value:2.83E-6|Clearance:0||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MTR3(YGR158C)|FD-Score:-3.33|P-value:4.35E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NAR1(YNL240C)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NAT2(YGR147C)|FD-Score:-4.09|P-value:2.15E-5|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NTF2(YER009W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.01||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:PRE2(YPR103W)|FD-Score:-3.74|P-value:9.05E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RFC3(YNL290W)|FD-Score:-3.34|P-value:4.22E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RHO1(YPR165W)|FD-Score:3.23|P-value:6.22E-4|Clearance:0.05||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIX7(YLL034C)|FD-Score:-3.2|P-value:6.75E-4|Clearance:0||SGD DESC:Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions Gene:RKI1(YOR095C)|FD-Score:3.75|P-value:8.83E-5|Clearance:0.23||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RNT1(YMR239C)|FD-Score:-5.17|P-value:1.15E-7|Clearance:0||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RPB5(YBR154C)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.11||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPN5(YDL147W)|FD-Score:4.03|P-value:2.74E-5|Clearance:0.2||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPP1(YHR062C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC23(YPR181C)|FD-Score:6.18|P-value:3.26E-10|Clearance:1.81||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SNP1(YIL061C)|FD-Score:3.84|P-value:6.27E-5|Clearance:0.04||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SNU23(YDL098C)|FD-Score:-3.11|P-value:9.27E-4|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPC110(YDR356W)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.04||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:SRP21(YKL122C)|FD-Score:-3.8|P-value:7.09E-5|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:TAF12(YDR145W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.25||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TUB2(YFL037W)|FD-Score:3.79|P-value:7.47E-5|Clearance:0.02||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:UTR5(YEL035C_p)|FD-Score:4.37|P-value:6.15E-6|Clearance:0.05||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YDL016C(YDL016C_d)|FD-Score:3.77|P-value:8.13E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YDR187C(YDR187C_d)|FD-Score:3.31|P-value:4.67E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YSH1(YLR277C)|FD-Score:-3.99|P-value:3.30E-5|Clearance:0||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:ARP4(YJL081C)|FD-Score:-3.76|P-value:8.39E-5|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BUR6(YER159C)|FD-Score:-7.35|P-value:1.01E-13|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CDC11(YJR076C)|FD-Score:-3.73|P-value:9.50E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC42(YLR229C)|FD-Score:-3.77|P-value:8.09E-5|Clearance:0||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:CDC60(YPL160W)|FD-Score:-4.59|P-value:2.21E-6|Clearance:0||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:ERG11(YHR007C)|FD-Score:4.32|P-value:7.67E-6|Clearance:0.29||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MET30(YIL046W)|FD-Score:-4.54|P-value:2.83E-6|Clearance:0||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:MTR3(YGR158C)|FD-Score:-3.33|P-value:4.35E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NAR1(YNL240C)|FD-Score:-3.52|P-value:2.13E-4|Clearance:0||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NAT2(YGR147C)|FD-Score:-4.09|P-value:2.15E-5|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NTF2(YER009W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.01||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:PRE2(YPR103W)|FD-Score:-3.74|P-value:9.05E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RFC3(YNL290W)|FD-Score:-3.34|P-value:4.22E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RHO1(YPR165W)|FD-Score:3.23|P-value:6.22E-4|Clearance:0.05||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIX7(YLL034C)|FD-Score:-3.2|P-value:6.75E-4|Clearance:0||SGD DESC:Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions Gene:RKI1(YOR095C)|FD-Score:3.75|P-value:8.83E-5|Clearance:0.23||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RNT1(YMR239C)|FD-Score:-5.17|P-value:1.15E-7|Clearance:0||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RPB5(YBR154C)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.11||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPN5(YDL147W)|FD-Score:4.03|P-value:2.74E-5|Clearance:0.2||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPP1(YHR062C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC23(YPR181C)|FD-Score:6.18|P-value:3.26E-10|Clearance:1.81||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SNP1(YIL061C)|FD-Score:3.84|P-value:6.27E-5|Clearance:0.04||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SNU23(YDL098C)|FD-Score:-3.11|P-value:9.27E-4|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPC110(YDR356W)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.04||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:SRP21(YKL122C)|FD-Score:-3.8|P-value:7.09E-5|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:TAF12(YDR145W)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.25||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TUB2(YFL037W)|FD-Score:3.79|P-value:7.47E-5|Clearance:0.02||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:UTR5(YEL035C_p)|FD-Score:4.37|P-value:6.15E-6|Clearance:0.05||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YDL016C(YDL016C_d)|FD-Score:3.77|P-value:8.13E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YDR187C(YDR187C_d)|FD-Score:3.31|P-value:4.67E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YSH1(YLR277C)|FD-Score:-3.99|P-value:3.30E-5|Clearance:0||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2859334
Download HOP data (tab-delimited text)  (excel)
Gene:ARP6(YLR085C)|FD-Score:5.67|P-value:7.24E-9||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BST1(YFL025C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD4(YJR092W)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p Gene:CCM1(YGR150C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CIN1(YOR349W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CTF19(YPL018W)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:EMP24(YGL200C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERG2(YMR202W)|FD-Score:4.45|P-value:4.29E-6||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERV14(YGL054C)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GCN20(YFR009W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCV1(YDR019C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GDH3(YAL062W)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GOT1(YMR292W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:HST1(YOL068C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HTZ1(YOL012C)|FD-Score:4.84|P-value:6.46E-7||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IRC18(YJL037W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:LAG1(YHL003C)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LTE1(YAL024C)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAL11(YGR289C)|FD-Score:-3.88|P-value:5.23E-5||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MND2(YIR025W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPL11(YDL202W)|FD-Score:4.77|P-value:9.10E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NOP12(YOL041C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OPT2(YPR194C)|FD-Score:-4.04|P-value:2.70E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAC1(YOR269W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly Gene:PAC2(YER007W)|FD-Score:6.09|P-value:5.55E-10||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PEP3(YLR148W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO89(YBR296C)|FD-Score:4.9|P-value:4.76E-7||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PHR1(YOR386W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIN2(YOR104W)|FD-Score:5.76|P-value:4.20E-9||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:QCR7(YDR529C)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:REF2(YDR195W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:REG1(YDR028C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RLF2(YPR018W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RSM28(YDR494W)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RTG1(YOL067C)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTT106(YNL206C)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SHE4(YOR035C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIP3(YNL257C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SIP4(YJL089W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SLX5(YDL013W)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF2(YOR290C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNT309(YPR101W)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPF1(YEL031W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSA3(YBL075C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STE50(YCL032W)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUR2(YDR297W)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SWR1(YDR334W)|FD-Score:4.88|P-value:5.42E-7||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TIR1(YER011W)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TSC3(YBR058C-A)|FD-Score:11.1|P-value:6.53E-29||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:7.35|P-value:1.01E-13||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBA4(YHR111W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBP12(YJL197W)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VPS30(YPL120W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS72(YDR485C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAF9(YNL107W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCR015C(YCR015C_p)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YGR127W(YGR127W_p)|FD-Score:-4.72|P-value:1.19E-6||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YJR111C(YJR111C_p)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YML122C(YML122C_d)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR209C(YMR209C_p)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL140C(YNL140C_p)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNR062C(YNR062C_p)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Putative membrane protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:5.22|P-value:8.93E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR059C(YOR059C_p)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YSP3(YOR003W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ARP6(YLR085C)|FD-Score:5.67|P-value:7.24E-9||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BST1(YFL025C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD4(YJR092W)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p Gene:CCM1(YGR150C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CIN1(YOR349W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CTF19(YPL018W)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:EMP24(YGL200C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERG2(YMR202W)|FD-Score:4.45|P-value:4.29E-6||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERV14(YGL054C)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GCN20(YFR009W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCV1(YDR019C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GDH3(YAL062W)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GOT1(YMR292W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:HST1(YOL068C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HTZ1(YOL012C)|FD-Score:4.84|P-value:6.46E-7||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IRC18(YJL037W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:LAG1(YHL003C)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LTE1(YAL024C)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAL11(YGR289C)|FD-Score:-3.88|P-value:5.23E-5||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MND2(YIR025W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPL11(YDL202W)|FD-Score:4.77|P-value:9.10E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NOP12(YOL041C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OPT2(YPR194C)|FD-Score:-4.04|P-value:2.70E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAC1(YOR269W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly Gene:PAC2(YER007W)|FD-Score:6.09|P-value:5.55E-10||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PEP3(YLR148W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO89(YBR296C)|FD-Score:4.9|P-value:4.76E-7||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PHR1(YOR386W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIN2(YOR104W)|FD-Score:5.76|P-value:4.20E-9||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:QCR7(YDR529C)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:REF2(YDR195W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:REG1(YDR028C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RLF2(YPR018W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RSM28(YDR494W)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RTG1(YOL067C)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTT106(YNL206C)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SHE4(YOR035C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIP3(YNL257C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SIP4(YJL089W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SLX5(YDL013W)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF2(YOR290C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNT309(YPR101W)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPF1(YEL031W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSA3(YBL075C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STE50(YCL032W)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUR2(YDR297W)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SWR1(YDR334W)|FD-Score:4.88|P-value:5.42E-7||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TIR1(YER011W)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TSC3(YBR058C-A)|FD-Score:11.1|P-value:6.53E-29||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:7.35|P-value:1.01E-13||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBA4(YHR111W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBP12(YJL197W)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VPS30(YPL120W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS72(YDR485C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAF9(YNL107W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCR015C(YCR015C_p)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YGR127W(YGR127W_p)|FD-Score:-4.72|P-value:1.19E-6||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YJR111C(YJR111C_p)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YML122C(YML122C_d)|FD-Score:4.02|P-value:2.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR209C(YMR209C_p)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL140C(YNL140C_p)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNR062C(YNR062C_p)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Putative membrane protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:5.22|P-value:8.93E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR059C(YOR059C_p)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YSP3(YOR003W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Putative precursor to the subtilisin-like protease III

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR181C6.183.26E-101.81SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YEL035C_p4.376.15E-60.05UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YHR007C4.327.67E-60.29ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDL147W4.032.74E-50.20RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YIL061C3.846.27E-50.04SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YFL037W3.797.47E-50.02TUB2Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
YDL016C_d3.778.13E-50.02YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YOR095C3.758.83E-50.23RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YHR062C3.522.12E-40.08RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YER133W3.442.87E-40.01GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YBR154C3.432.98E-40.11RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YER009W3.324.45E-40.01NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YDR187C_d3.314.67E-40.04YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YDR356W3.275.33E-40.04SPC110Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
YPR165W3.236.22E-40.05RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A11.106.53E-29TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML124C7.351.01E-13TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YER007W6.095.55E-10PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YOR104W5.764.20E-9PIN2Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated
YLR085C5.677.24E-9ARP6Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YOL047C_p5.228.93E-8YOL047C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YCL032W5.191.06E-7STE50Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
YBR296C4.904.76E-7PHO89Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
YDR334W4.885.42E-7SWR1Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YOL012C4.846.46E-7HTZ1Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
YDL202W4.779.10E-7MRPL11Mitochondrial ribosomal protein of the large subunit
YNL257C4.741.09E-6SIP3Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
YOR034C-A_p4.641.76E-6YOR034C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YMR202W4.454.29E-6ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YPR101W4.434.64E-6SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p

GO enrichment analysis for SGTC_2161
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3304.42E-150SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.555556tubulin folding & SWR complex
0.3244.85E-144SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.107143
0.3068.25E-128SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.512821tubulin folding & SWR complex
0.2924.28E-116SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0689655TSC3-RPN4
0.2907.02E-114SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.161765
0.2706.63E-99SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.2
0.2678.74E-97SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.203704
0.2601.13E-91SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.117647
0.2481.25E-83SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0517241TSC3-RPN4
0.2417.13E-79SGTC_229benomyl 22.9 μMMiscellaneous287800.0882353tubulin folding & SWR complex
0.2391.87E-77SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.132353
0.2361.38E-75SGTC_2123807-4682 38.8 μMChemDiv (Drug-like library)31255010.138462tubulin folding & SWR complex
0.2311.38E-72SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.21875TSC3-RPN4
0.2307.62E-72SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0701754TSC3-RPN4
0.2301.24E-71SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.131148TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2103537813810 μM0.6244142Chembridge (Fragment library)241.242040.84403
SGTC_21735763948167 μM0.5897442867177Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_214156216387.67 μM0.5555562856238Chembridge (Fragment library)289.713681.71303tubulin folding & SWR complex
SGTC_2200660060421.91 μM0.5128212904777Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_14863970-07934.53 μM0.4871792844330ChemDiv (Drug-like library)320.13811.59203
SGTC_14873970-07952.82 μM0.476193765103ChemDiv (Drug-like library)320.13811.59203DNA intercalators
SGTC_215856571233.62 μM0.4523812858827Chembridge (Fragment library)269.29521.81603
SGTC_1903970-07903.72 μM0.441862832352ChemDiv (Drug-like library)286.23960.73805
SGTC_9963970-07991.8 μM0.4042556615648ChemDiv (Drug-like library)299.321181.704
SGTC_2470566481721.68 μM0.3518522859561Miscellaneous361.799483.36215