ketoconazole FDA approved compound

1-[4-[4-[[(2R,4S)-2-(2,4-dichlorophenyl)-2-(imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy]phenyl]piperazin-1-yl]ethanone


Established Yeast Drug Target : ERG11

Ketoconazole is an imidazole antifungal; acts by inhibiting lanosterol 14α-demethylase preventing the conversion of lanosterol to ergosterol, an essential component of the fungal cytoplasmic membrane.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_217
Screen concentration 989.0 nM
Source Miscellaneous
PubChem CID 456201
SMILES CC(=O)N1CCN(CC1)C2=CC=C(C=C2)OCC3COC(O3)(CN4C=CN=C4)C5=C(C=C(C=C5)Cl)Cl
Standardized SMILES CC(=O)N1CCN(CC1)c2ccc(OCC3COC(Cn4ccnc4)(O3)c5ccc(Cl)cc5Cl)cc2
Molecular weight 531.4309
ALogP 3.61
H-bond donor count 0
H-bond acceptor count 6
Response signature ergosterol depletion effects on membrane

Pool Growth Kinetics
% growth inhibition (Het. pool) 55.79
% growth inhibition (Hom. pool) 22.93


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 456201
Download HIP data (tab-delimited text)  (excel)
Gene:BRL1(YHR036W)|FD-Score:6.54|P-value:3.13E-11|Clearance:1.23||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC20(YGL116W)|FD-Score:3.83|P-value:6.54E-5|Clearance:0.01||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC36(YDL165W)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.16||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC39(YCR093W)|FD-Score:5.31|P-value:5.53E-8|Clearance:0.67||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DBP2(YNL112W)|FD-Score:6.63|P-value:1.73E-11|Clearance:0.09||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DED1(YOR204W)|FD-Score:7.13|P-value:4.90E-13|Clearance:0.12||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:HEM1(YDR232W)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.03||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:HEM2(YGL040C)|FD-Score:3.82|P-value:6.76E-5|Clearance:0.2||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:IDI1(YPL117C)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.32||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:MCM5(YLR274W)|FD-Score:-3.25|P-value:5.82E-4|Clearance:0||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MED4(YOR174W)|FD-Score:9.47|P-value:1.46E-21|Clearance:2.33||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NCP1(YHR042W)|FD-Score:4.64|P-value:1.78E-6|Clearance:0.01||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:POL30(YBR088C)|FD-Score:-4.01|P-value:2.99E-5|Clearance:0||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:RAD53(YPL153C)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.17||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RHO3(YIL118W)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPT6(YGL048C)|FD-Score:-3.16|P-value:7.89E-4|Clearance:0||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC8(YFR037C)|FD-Score:3.45|P-value:2.85E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SPT14(YPL175W)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.08||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRP72(YPL210C)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TIM44(YIL022W)|FD-Score:-3.77|P-value:8.18E-5|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:TIM54(YJL054W)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.03||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:YBR124W(YBR124W_d)|FD-Score:-4.23|P-value:1.17E-5|Clearance:0||SGD DESC:Putative protein of unknown function Gene:YDL196W(YDL196W_d)|FD-Score:7.02|P-value:1.14E-12|Clearance:0.39||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL195C(YJL195C_d)|FD-Score:4.6|P-value:2.13E-6|Clearance:0.41||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YOL134C(YOL134C_d)|FD-Score:3.1|P-value:9.56E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:YOR203W(YOR203W_d)|FD-Score:3.32|P-value:4.55E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:BRL1(YHR036W)|FD-Score:6.54|P-value:3.13E-11|Clearance:1.23||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC20(YGL116W)|FD-Score:3.83|P-value:6.54E-5|Clearance:0.01||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC36(YDL165W)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.16||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC39(YCR093W)|FD-Score:5.31|P-value:5.53E-8|Clearance:0.67||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DBP2(YNL112W)|FD-Score:6.63|P-value:1.73E-11|Clearance:0.09||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DED1(YOR204W)|FD-Score:7.13|P-value:4.90E-13|Clearance:0.12||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:HEM1(YDR232W)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.03||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:HEM2(YGL040C)|FD-Score:3.82|P-value:6.76E-5|Clearance:0.2||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:IDI1(YPL117C)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.32||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:MCM5(YLR274W)|FD-Score:-3.25|P-value:5.82E-4|Clearance:0||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:MED4(YOR174W)|FD-Score:9.47|P-value:1.46E-21|Clearance:2.33||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NCP1(YHR042W)|FD-Score:4.64|P-value:1.78E-6|Clearance:0.01||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:POL30(YBR088C)|FD-Score:-4.01|P-value:2.99E-5|Clearance:0||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:RAD53(YPL153C)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.17||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RHO3(YIL118W)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPT6(YGL048C)|FD-Score:-3.16|P-value:7.89E-4|Clearance:0||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC8(YFR037C)|FD-Score:3.45|P-value:2.85E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SPT14(YPL175W)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.08||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRP72(YPL210C)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TIM44(YIL022W)|FD-Score:-3.77|P-value:8.18E-5|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:TIM54(YJL054W)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.03||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:YBR124W(YBR124W_d)|FD-Score:-4.23|P-value:1.17E-5|Clearance:0||SGD DESC:Putative protein of unknown function Gene:YDL196W(YDL196W_d)|FD-Score:7.02|P-value:1.14E-12|Clearance:0.39||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL195C(YJL195C_d)|FD-Score:4.6|P-value:2.13E-6|Clearance:0.41||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YOL134C(YOL134C_d)|FD-Score:3.1|P-value:9.56E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:YOR203W(YOR203W_d)|FD-Score:3.32|P-value:4.55E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 456201
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:-4.39|P-value:5.73E-6||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AFT1(YGL071W)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC2(YCR082W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:APL2(YKL135C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASN1(YPR145W)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BRE2(YLR015W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BTS1(YPL069C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD27(YFL023W)|FD-Score:-3.77|P-value:8.08E-5||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAF120(YNL278W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR2(YHR057C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CSG2(YBR036C)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-4.76|P-value:9.79E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYS4(YGR155W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAP1(YPL170W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DBF2(YGR092W)|FD-Score:-3.86|P-value:5.58E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DLT1(YMR126C_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:DUR1,2(YBR208C)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress Gene:ECM33(YBR078W)|FD-Score:8.25|P-value:7.94E-17||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERD1(YDR414C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERV14(YGL054C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ETT1(YOR051C)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FYV10(YIL097W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:FYV7(YLR068W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GAL1(YBR020W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCS1(YDL226C)|FD-Score:5.4|P-value:3.37E-8||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GET2(YER083C)|FD-Score:-4.79|P-value:8.18E-7||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRE3(YHR104W)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HDA2(YDR295C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:HOP2(YGL033W)|FD-Score:-5.13|P-value:1.46E-7||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HOS4(YIL112W)|FD-Score:-3.91|P-value:4.55E-5||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:HUR1(YGL168W)|FD-Score:-5.57|P-value:1.31E-8||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HXT12(YIL170W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:HXT4(YHR092C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:ICS3(YJL077C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:ICT1(YLR099C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:ICY1(YMR195W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IMP2'(YIL154C)|FD-Score:5.75|P-value:4.57E-9||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC21(YMR073C)|FD-Score:-4.76|P-value:9.74E-7||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRE1(YHR079C)|FD-Score:4.86|P-value:5.82E-7||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:IRS4(YKR019C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISU1(YPL135W)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:ISU2(YOR226C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:JID1(YPR061C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:JNM1(YMR294W)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LCL1(YPL056C_p)|FD-Score:-3.73|P-value:9.45E-5||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:MAC1(YMR021C)|FD-Score:-5.22|P-value:8.93E-8||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MPA43(YNL249C)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRP17(YKL003C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MRPL15(YLR312W-A)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL22(YNL177C)|FD-Score:-3.78|P-value:7.90E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:-3.73|P-value:9.46E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSW1(YDR268W)|FD-Score:-7.55|P-value:2.20E-14||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTC4(YBR255W)|FD-Score:-4.55|P-value:2.69E-6||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:PCL9(YDL179W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PET123(YOR158W)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PHO86(YJL117W)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIM1(YBL022C)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PMR1(YGL167C)|FD-Score:-3.98|P-value:3.43E-5||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POP2(YNR052C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PPT2(YPL148C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD26(YJR035W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RAS1(YOR101W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:6.47|P-value:4.91E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:4.47|P-value:3.95E-6||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM8(YGL045W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPL19A(YBR084C-A)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL42B(YHR141C)|FD-Score:-5.14|P-value:1.41E-7||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:-3.97|P-value:3.52E-5||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RTT10(YPL183C)|FD-Score:5.46|P-value:2.32E-8||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:RTT103(YDR289C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SCJ1(YMR214W)|FD-Score:-8.94|P-value:2.00E-19||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SEC72(YLR292C)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SKP2(YNL311C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLM1(YIL105C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SMF1(YOL122C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins Gene:SNF2(YOR290C)|FD-Score:-3.72|P-value:9.78E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPT2(YER161C)|FD-Score:-4.53|P-value:2.96E-6||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SPT8(YLR055C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRF1(YDL133W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSN2(YDR443C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SUM1(YDR310C)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUR4(YLR372W)|FD-Score:-4.9|P-value:4.70E-7||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWR1(YDR334W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYS1(YJL004C)|FD-Score:-3.94|P-value:4.08E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA3(YHR009C_p)|FD-Score:-5.75|P-value:4.36E-9||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TDH3(YGR192C)|FD-Score:-4.29|P-value:8.91E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TIM18(YOR297C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex Gene:TMA46(YOR091W)|FD-Score:-4.35|P-value:6.86E-6||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRP4(YDR354W)|FD-Score:-5.1|P-value:1.72E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS33(YOR115C)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TUF1(YOR187W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TVP18(YMR071C)|FD-Score:5.72|P-value:5.22E-9||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:URM1(YIL008W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:UTR1(YJR049C)|FD-Score:7.7|P-value:6.81E-15||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VID30(YGL227W)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VMA1(YDL185W)|FD-Score:-4.37|P-value:6.29E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-4.76|P-value:9.60E-7||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:-4.63|P-value:1.87E-6||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS33(YLR396C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:VPS45(YGL095C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS74(YDR372C)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YCK1(YHR135C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YDL073W(YDL073W_p)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR154C(YDR154C_d)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YDR391C(YDR391C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YFL034W(YFL034W_p)|FD-Score:5.65|P-value:7.87E-9||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YFT2(YDR319C_p)|FD-Score:4.88|P-value:5.30E-7||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR122W(YGR122W)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR176W(YGR176W_d)|FD-Score:5.52|P-value:1.71E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR045W(YHR045W_p)|FD-Score:6.17|P-value:3.44E-10||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YIM1(YMR152W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YLR050C(YLR050C_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YLR171W(YLR171W_d)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR255C(YLR255C_d)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR271W(YLR271W_p)|FD-Score:7.15|P-value:4.37E-13||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR407W(YLR407W_p)|FD-Score:-3.24|P-value:5.87E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YML122C(YML122C_d)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR114C(YMR114C)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR124W(YMR124W_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR155W(YMR155W_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNL198C(YNL198C_d)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR071C(YNR071C_p)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Putative aldose 1-epimerase Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-7.25|P-value:2.09E-13||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR379C(YOR379C_d)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPL077C(YPL077C_p)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL225W(YPL225W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR064W(YPR064W_d)|FD-Score:-4.51|P-value:3.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR130C(YPR130C_d)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPS7(YDR349C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YPT31(YER031C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ADO1(YJR105W)|FD-Score:-4.39|P-value:5.73E-6||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AFT1(YGL071W)|FD-Score:-4.12|P-value:1.92E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC2(YCR082W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:APL2(YKL135C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASN1(YPR145W)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BRE2(YLR015W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BTS1(YPL069C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD27(YFL023W)|FD-Score:-3.77|P-value:8.08E-5||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAF120(YNL278W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR2(YHR057C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CSG2(YBR036C)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-4.76|P-value:9.79E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYS4(YGR155W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAP1(YPL170W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DBF2(YGR092W)|FD-Score:-3.86|P-value:5.58E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DLT1(YMR126C_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:DUR1,2(YBR208C)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress Gene:ECM33(YBR078W)|FD-Score:8.25|P-value:7.94E-17||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERD1(YDR414C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERV14(YGL054C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ETT1(YOR051C)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FYV10(YIL097W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:FYV7(YLR068W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GAL1(YBR020W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCS1(YDL226C)|FD-Score:5.4|P-value:3.37E-8||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GET2(YER083C)|FD-Score:-4.79|P-value:8.18E-7||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRE3(YHR104W)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HDA2(YDR295C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:HOP2(YGL033W)|FD-Score:-5.13|P-value:1.46E-7||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HOS4(YIL112W)|FD-Score:-3.91|P-value:4.55E-5||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:HUR1(YGL168W)|FD-Score:-5.57|P-value:1.31E-8||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HXT12(YIL170W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:HXT4(YHR092C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication Gene:ICS3(YJL077C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:ICT1(YLR099C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:ICY1(YMR195W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IMP2'(YIL154C)|FD-Score:5.75|P-value:4.57E-9||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC21(YMR073C)|FD-Score:-4.76|P-value:9.74E-7||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRE1(YHR079C)|FD-Score:4.86|P-value:5.82E-7||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:IRS4(YKR019C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISU1(YPL135W)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:ISU2(YOR226C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:JID1(YPR061C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:JNM1(YMR294W)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LCL1(YPL056C_p)|FD-Score:-3.73|P-value:9.45E-5||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:MAC1(YMR021C)|FD-Score:-5.22|P-value:8.93E-8||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MPA43(YNL249C)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRP17(YKL003C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MRPL15(YLR312W-A)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL22(YNL177C)|FD-Score:-3.78|P-value:7.90E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:-3.73|P-value:9.46E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSW1(YDR268W)|FD-Score:-7.55|P-value:2.20E-14||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTC4(YBR255W)|FD-Score:-4.55|P-value:2.69E-6||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:PCL9(YDL179W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PET123(YOR158W)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PHO86(YJL117W)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIM1(YBL022C)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PMR1(YGL167C)|FD-Score:-3.98|P-value:3.43E-5||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POP2(YNR052C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PPT2(YPL148C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD26(YJR035W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RAS1(YOR101W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:6.47|P-value:4.91E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:4.47|P-value:3.95E-6||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM8(YGL045W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPL19A(YBR084C-A)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL42B(YHR141C)|FD-Score:-5.14|P-value:1.41E-7||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RPS11B(YBR048W)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:-3.97|P-value:3.52E-5||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RTT10(YPL183C)|FD-Score:5.46|P-value:2.32E-8||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:RTT103(YDR289C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SCJ1(YMR214W)|FD-Score:-8.94|P-value:2.00E-19||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SEC72(YLR292C)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SKP2(YNL311C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLM1(YIL105C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SMF1(YOL122C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins Gene:SNF2(YOR290C)|FD-Score:-3.72|P-value:9.78E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPT2(YER161C)|FD-Score:-4.53|P-value:2.96E-6||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SPT8(YLR055C)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRF1(YDL133W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSN2(YDR443C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SUM1(YDR310C)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUR4(YLR372W)|FD-Score:-4.9|P-value:4.70E-7||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWR1(YDR334W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYS1(YJL004C)|FD-Score:-3.94|P-value:4.08E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA3(YHR009C_p)|FD-Score:-5.75|P-value:4.36E-9||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TDH3(YGR192C)|FD-Score:-4.29|P-value:8.91E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TIM18(YOR297C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex Gene:TMA46(YOR091W)|FD-Score:-4.35|P-value:6.86E-6||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRP4(YDR354W)|FD-Score:-5.1|P-value:1.72E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS33(YOR115C)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TUF1(YOR187W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TVP18(YMR071C)|FD-Score:5.72|P-value:5.22E-9||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:URM1(YIL008W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:UTR1(YJR049C)|FD-Score:7.7|P-value:6.81E-15||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VID30(YGL227W)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VMA1(YDL185W)|FD-Score:-4.37|P-value:6.29E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-4.76|P-value:9.60E-7||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:-4.63|P-value:1.87E-6||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS33(YLR396C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:VPS45(YGL095C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:VPS74(YDR372C)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YCK1(YHR135C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YDL073W(YDL073W_p)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDL118W(YDL118W_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR154C(YDR154C_d)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YDR391C(YDR391C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YFL034W(YFL034W_p)|FD-Score:5.65|P-value:7.87E-9||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YFT2(YDR319C_p)|FD-Score:4.88|P-value:5.30E-7||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR122W(YGR122W)|FD-Score:4.98|P-value:3.20E-7||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR176W(YGR176W_d)|FD-Score:5.52|P-value:1.71E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR045W(YHR045W_p)|FD-Score:6.17|P-value:3.44E-10||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YIM1(YMR152W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YLR050C(YLR050C_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YLR171W(YLR171W_d)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR255C(YLR255C_d)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR271W(YLR271W_p)|FD-Score:7.15|P-value:4.37E-13||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR407W(YLR407W_p)|FD-Score:-3.24|P-value:5.87E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YML122C(YML122C_d)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR114C(YMR114C)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YMR124W(YMR124W_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR155W(YMR155W_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNL198C(YNL198C_d)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR071C(YNR071C_p)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Putative aldose 1-epimerase Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-7.25|P-value:2.09E-13||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR379C(YOR379C_d)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPL077C(YPL077C_p)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL225W(YPL225W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR064W(YPR064W_d)|FD-Score:-4.51|P-value:3.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR130C(YPR130C_d)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPS7(YDR349C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YPT31(YER031C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR174W9.471.46E-212.33MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YOR204W7.134.90E-130.12DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YDL196W_d7.021.14E-120.39YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YNL112W6.631.73E-110.09DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YHR036W6.543.13E-111.23BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YCR093W5.315.53E-80.67CDC39Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YHR042W4.641.78E-60.01NCP1NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
YDR232W4.631.85E-60.03HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YJL195C_d4.602.13E-60.41YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YJL054W4.181.44E-50.03TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
YPL117C4.151.67E-50.32IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
YGL116W3.836.54E-50.01CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YGL040C3.826.76E-50.20HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YPL153C3.611.52E-40.17RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YFR037C3.452.85E-40.05RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR078W8.257.94E-17ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YJR049C7.706.81E-15UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YLR271W_p7.154.37E-13YLR271W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YJL204C6.474.91E-11RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YHR045W_p6.173.44E-10YHR045W_pPutative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
YPL135W5.822.91E-9ISU1Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable
YIL154C5.754.57E-9IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YMR071C5.725.22E-9TVP18Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
YFL034W_p5.657.87E-9YFL034W_pPutative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
YGR176W_d5.521.71E-8YGR176W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL183C5.462.32E-8RTT10WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
YDL226C5.403.37E-8GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YGR122W4.983.20E-7YGR122WProtein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
YDR319C_p4.885.30E-7YFT2_pProtein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
YHR079C4.865.82E-7IRE1Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress

GO enrichment analysis for SGTC_217
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3089.81E-130SGTC_256clotrimazole 625.0 nMMiscellaneous28120.159091ergosterol depletion effects on membrane
0.3082.03E-129SGTC_227fluconazole 33.5 μMMiscellaneous33650.09375azole & statin
0.2413.24E-78SGTC_252miconazole 1.3 nMMiscellaneous41890.272727ergosterol depletion effects on membrane
0.2394.16E-77SGTC_3204092-0846 5.3 μMChemDiv (Drug-like library)13593560.127451ergosterol depletion effects on membrane
0.2361.41E-75SGTC_6890833-0039 98.0 μMChemDiv (Drug-like library)53747260.0843373ergosterol depletion effects on membrane
0.2261.03E-68SGTC_3464513-0042 7.2 μMChemDiv (Drug-like library)28273720.255319azole & statin
0.2082.34E-58SGTC_57miconazole 162.5 nMChemDiv (Drug-like library)41890.272727ergosterol depletion effects on membrane
0.1945.00E-51SGTC_2778tioconazole 65.1 nMMiscellaneous54820.255319ergosterol depletion effects on membrane
0.1946.46E-51SGTC_10194262-0212 3.4 μMChemDiv (Drug-like library)46772130.0808081ergosterol depletion effects on membrane
0.1911.13E-49SGTC_262fenpropimorph 62.5 μMMiscellaneous916950.0927835ergosterol depletion effects on membrane
0.1864.02E-47SGTC_218alverine citrate 93.8 μMMiscellaneous217180.0568182fatty acid desaturase (OLE1)
0.1808.50E-44SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.144578plasma membrane duress
0.1763.26E-42SGTC_2014262-0159 12.8 μMChemDiv (Drug-like library)13410290.0967742ergosterol depletion effects on membrane
0.1731.32E-40SGTC_1788fluconazole 20.0 μMMiscellaneous33650.09375azole & statin
0.1676.18E-38SGTC_1072oxiconazole 14.9 nMNIH Clinical Collection473160.277778ergosterol depletion effects on membrane

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1066ketoconazole628 nM1456201NIH Clinical Collection531.430923.6106
SGTC_1076itraconazole331 nM0.5408163793Miscellaneous705.633426.43409
SGTC_1072oxiconazole14.9 nM0.27777847316NIH Clinical Collection492.140164.26816ergosterol depletion effects on membrane
SGTC_252miconazole1.31 nM0.2727274189Miscellaneous416.128565.64502ergosterol depletion effects on membrane
SGTC_57miconazole162.5 nM0.2727274189ChemDiv (Drug-like library)416.128565.64502ergosterol depletion effects on membrane
SGTC_3214534-389065.3 μM0.268817699746ChemDiv (Drug-like library)345.82333.24704RPP1 & pyrimidine depletion
SGTC_2861902459519.48 μM0.26041716482810Chembridge (Drug-like library)401.886562.9914
SGTC_3464513-00427.16 μM0.2553192827372ChemDiv (Drug-like library)352.38383.07105azole & statin
SGTC_2778tioconazole65.09 nM0.2553195482Miscellaneous387.711224.43703ergosterol depletion effects on membrane
SGTC_3146909560949.47 μM0.25531917173999Chembridge (Drug-like library)380.268363.9104
SGTC_2777climbazole1.54 μM0.25287437907Miscellaneous292.760683.76603iron homeostasis