5807670

2-benzylsulfanyl-1H-pyrimidin-6-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2175
Screen concentration 165.3 μM
Source Chembridge (Fragment library)
PubChem CID 241186
SMILES C1=CC=C(C=C1)CSC2=NC=CC(=O)N2
Standardized SMILES Oc1ccnc(SCc2ccccc2)n1
Molecular weight 218.2749
ALogP 2.04
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.64
% growth inhibition (Hom. pool) 8.66


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 241186
Download HIP data (tab-delimited text)  (excel)
Gene:CWC25(YNL245C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.13||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:RRP15(YPR143W)|FD-Score:3.75|P-value:8.98E-5|Clearance:0.14||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RSC8(YFR037C)|FD-Score:-4.83|P-value:6.82E-7|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC13(YLR208W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.26||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC63(YOR254C)|FD-Score:4.33|P-value:7.30E-6|Clearance:0.59||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPT5(YML010W)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.31||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:TFC3(YAL001C)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:CWC25(YNL245C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.13||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:RRP15(YPR143W)|FD-Score:3.75|P-value:8.98E-5|Clearance:0.14||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RSC8(YFR037C)|FD-Score:-4.83|P-value:6.82E-7|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC13(YLR208W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.26||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC63(YOR254C)|FD-Score:4.33|P-value:7.30E-6|Clearance:0.59||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPT5(YML010W)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.31||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:TFC3(YAL001C)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 241186
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ACF4(YJR083C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADH4(YGL256W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:APL2(YKL135C)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARO1(YDR127W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG15(YCR068W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:AVT3(YKL146W)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BLS1(YLR408C_p)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BUD2(YKL092C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CBP2(YHL038C)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:CHO2(YGR157W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CLC1(YGR167W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:DAL81(YIR023W)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EMC5(YIL027C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:EOS1(YNL080C)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERP5(YHR110W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCN20(YFR009W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:7.97|P-value:8.08E-16||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDB1(YPR184W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEP4(YHR100C)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GTR2(YGR163W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HRB1(YNL004W)|FD-Score:4.51|P-value:3.18E-6||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:HUG1(YML058W-A)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IRC7(YFR055W_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:KRE28(YDR532C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:KTR7(YIL085C)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MCK1(YNL307C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDJ2(YNL328C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MED1(YPR070W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MPA43(YNL249C)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPL31(YKL138C)|FD-Score:-3.8|P-value:7.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS11(YMR164C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:PAR32(YDL173W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEX31(YGR004W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PKH3(YDR466W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:RLM1(YPL089C)|FD-Score:-3.76|P-value:8.49E-5||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPS25A(YGR027C)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RTT103(YDR289C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAP4(YGL229C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCW11(YGL028C)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SVS1(YPL163C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TAH1(YCR060W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:TRM82(YDR165W)|FD-Score:-4.54|P-value:2.83E-6||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP1(YDR007W)|FD-Score:6.92|P-value:2.23E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7.2|P-value:3.03E-13||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:10.2|P-value:8.65E-25||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.41|P-value:2.00E-17||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRX1(YLR043C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBP15(YMR304W)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:VMA5(YKL080W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS52(YDR484W)|FD-Score:-5.18|P-value:1.11E-7||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YAL066W(YAL066W_d)|FD-Score:-7.51|P-value:2.98E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YAR047C(YAR047C_d)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL053W(YBL053W_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR277C(YBR277C_d)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR001W(YCR001W_d)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:6.5|P-value:4.15E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YGL117W(YGL117W_p)|FD-Score:5.47|P-value:2.30E-8||SGD DESC:Putative protein of unknown function Gene:YGL152C(YGL152C_d)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W Gene:YGL217C(YGL217C_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGR064W(YGR064W_d)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR151C(YGR151C_d)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YGR290W(YGR290W_d)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YHL042W(YHL042W_p)|FD-Score:5.33|P-value:4.86E-8||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YKL147C(YKL147C_d)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YLR217W(YLR217W_d)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YML009C-A(YML009C-A_d)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML096W(YML096W_p)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YNL205C(YNL205C_d)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL235C(YNL235C_d)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNL296W(YNL296W_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOR082C(YOR082C_d)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPL071C(YPL071C_p)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPR196W(YPR196W_p)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:ABZ2(YMR289W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ACF4(YJR083C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADH4(YGL256W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:APL2(YKL135C)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARO1(YDR127W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG15(YCR068W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:AVT3(YKL146W)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BLS1(YLR408C_p)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BUD2(YKL092C)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint Gene:CBP2(YHL038C)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:CHO2(YGR157W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CLC1(YGR167W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:DAL81(YIR023W)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EMC5(YIL027C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:EOS1(YNL080C)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERP5(YHR110W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCN20(YFR009W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:7.97|P-value:8.08E-16||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDB1(YPR184W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GEP4(YHR100C)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GTR2(YGR163W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HRB1(YNL004W)|FD-Score:4.51|P-value:3.18E-6||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:HUG1(YML058W-A)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IRC7(YFR055W_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:KRE28(YDR532C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:KTR7(YIL085C)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MCK1(YNL307C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MDJ2(YNL328C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MED1(YPR070W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MPA43(YNL249C)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPL31(YKL138C)|FD-Score:-3.8|P-value:7.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS11(YMR164C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:PAR32(YDL173W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEX31(YGR004W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PKH3(YDR466W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:RLM1(YPL089C)|FD-Score:-3.76|P-value:8.49E-5||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPS25A(YGR027C)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RTT103(YDR289C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAP4(YGL229C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCW11(YGL028C)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SVS1(YPL163C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TAH1(YCR060W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:TRM82(YDR165W)|FD-Score:-4.54|P-value:2.83E-6||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP1(YDR007W)|FD-Score:6.92|P-value:2.23E-12||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7.2|P-value:3.03E-13||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:10.2|P-value:8.65E-25||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.41|P-value:2.00E-17||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRX1(YLR043C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBP15(YMR304W)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:VMA5(YKL080W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS52(YDR484W)|FD-Score:-5.18|P-value:1.11E-7||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YAL066W(YAL066W_d)|FD-Score:-7.51|P-value:2.98E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YAR047C(YAR047C_d)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL053W(YBL053W_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR277C(YBR277C_d)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR001W(YCR001W_d)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:6.5|P-value:4.15E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YGL117W(YGL117W_p)|FD-Score:5.47|P-value:2.30E-8||SGD DESC:Putative protein of unknown function Gene:YGL152C(YGL152C_d)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W Gene:YGL217C(YGL217C_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGR064W(YGR064W_d)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR151C(YGR151C_d)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YGR290W(YGR290W_d)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YHL042W(YHL042W_p)|FD-Score:5.33|P-value:4.86E-8||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:YKL147C(YKL147C_d)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Gene:YLR217W(YLR217W_d)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YML009C-A(YML009C-A_d)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML096W(YML096W_p)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YNL205C(YNL205C_d)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL235C(YNL235C_d)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNL296W(YNL296W_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOR082C(YOR082C_d)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPL071C(YPL071C_p)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPR196W(YPR196W_p)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Putative maltose-responsive transcription factor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR254C4.337.30E-60.59SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YPR143W3.758.98E-50.14RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YNL245C3.611.55E-40.13CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YLR208W3.472.56E-40.26SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YML010W3.216.56E-40.31SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YKL082C2.910.001820.30RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YDR236C2.610.004570.08FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YJR045C2.520.005820.09SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YFR050C2.430.007460.01PRE4Beta 7 subunit of the 20S proteasome
YLR008C2.430.007600.07PAM18Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
YNL251C2.360.009220.04NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YIL078W2.310.010400.01THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YNL088W2.300.010800.02TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YFR029W2.280.011200.05PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YER018C2.240.012700.01SPC25Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C10.208.65E-25TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W8.412.00E-17TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YEL009C7.978.08E-16GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YER090W7.203.03E-13TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR007W6.922.23E-12TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR008C_d6.562.70E-11YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR056C_p6.504.15E-11YDR056C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein
YGL117W_p5.472.30E-8YGL117W_pPutative protein of unknown function
YHL042W_p5.334.86E-8YHL042W_pPutative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
YIR023W5.022.60E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YNL235C_d4.798.32E-7YNL235C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
YNL307C4.592.26E-6MCK1Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family
YDR127W4.572.44E-6ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YGR151C_d4.523.10E-6YGR151C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress
YNL004W4.513.18E-6HRB1Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2175
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4278.41E-261SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0615385
0.4091.09E-236SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0675676
0.3995.81E-224SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0615385
0.3922.12E-216SGTC_24885268135 45.7 μMMiscellaneous12703560.188679
0.3844.20E-206SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0243902
0.3821.39E-204SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0925926
0.3811.29E-203SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0392157
0.3817.48E-203SGTC_18825615643 20.0 μMMiscellaneous22530750.08TRP & mitochondrial translation
0.3763.18E-197SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.118644
0.3693.93E-190SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0657895
0.3657.59E-186SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.16129
0.3653.21E-185SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.0181818
0.3581.57E-178SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.0547945
0.3581.41E-177SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0454545
0.3551.42E-174SGTC_24785763493 32.3 μMMiscellaneous13655760.114754

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2237666774747.22 μM0.72973729940Chembridge (Fragment library)236.2653632.24314
SGTC_2238667162073.24 μM0.72973729941Chembridge (Fragment library)232.301482.52413
SGTC_22927671323168.05 μM0.36937504Chembridge (Fragment library)251.2800032.02324RNA processing & uracil transport
SGTC_5643555-0129330 μM0.352941563260ChemDiv (Drug-like library)297.414563.66803
SGTC_23307979373200 μM0.3469391863007Chembridge (Fragment library)247.316122.0241460S ribosome export
SGTC_2351902685872.31 μM0.2857141409300Chembridge (Fragment library)231.250520.87923RNA processing & uracil transport
SGTC_23427990637118.86 μM0.2641512033102Chembridge (Fragment library)242.23341.07123RNA processing & uracil transport
SGTC_415dibenzyl ketone100 μM0.2631587593Miscellaneous210.271063.29401
SGTC_3256913890249.47 μM0.25925917228773Chembridge (Drug-like library)305.373663.7321PDR1
SGTC_14163966-032125.3 μM0.255319582530ChemDiv (Drug-like library)252.267981.8312