6298986

2-amino-[1,3,5]triazino[2,1-b][1,3]benzoxazole-4-thione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2179
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 738374
SMILES C1=CC=C2C(=C1)N3C(=NC(=NC3=S)N)O2
Standardized SMILES NC1=NC(=S)N2C(=N1)Oc3ccccc23
Molecular weight 218.2351
ALogP 2.73
H-bond donor count 1
H-bond acceptor count 2
Response signature heme requiring

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.26
% growth inhibition (Hom. pool) 5.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 738374
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:3.83|P-value:6.41E-5|Clearance:0.03||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ACT1(YFL039C)|FD-Score:-3.2|P-value:6.97E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT3(YJL014W)|FD-Score:-3.1|P-value:9.72E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDS1(YBR029C)|FD-Score:-3.19|P-value:7.11E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:ENP1(YBR247C)|FD-Score:-4.28|P-value:9.52E-6|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:HEM12(YDR047W)|FD-Score:5.36|P-value:4.09E-8|Clearance:0.68||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MCM7(YBR202W)|FD-Score:-4.04|P-value:2.70E-5|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NOG1(YPL093W)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP1(YDL014W)|FD-Score:-3.14|P-value:8.56E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NSL1(YPL233W)|FD-Score:3.3|P-value:4.78E-4|Clearance:0.24||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:PTR3(YFR029W)|FD-Score:4.15|P-value:1.63E-5|Clearance:0.32||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RAT1(YOR048C)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RLP24(YLR009W)|FD-Score:-4.19|P-value:1.39E-5|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPL10(YLR075W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL18A(YOL120C)|FD-Score:-4.95|P-value:3.70E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL25(YOL127W)|FD-Score:-5.25|P-value:7.77E-8|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL5(YPL131W)|FD-Score:-5.51|P-value:1.76E-8|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPO31(YOR116C)|FD-Score:4.62|P-value:1.89E-6|Clearance:0.47||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:-4.09|P-value:2.20E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPR2(YIR015W)|FD-Score:4.69|P-value:1.40E-6|Clearance:0.06||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPS2(YGL123W)|FD-Score:-5.47|P-value:2.30E-8|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.12|P-value:9.14E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:-7.22|P-value:2.64E-13|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC1(YDR164C)|FD-Score:-3.09|P-value:9.92E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SSL1(YLR005W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.11||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SUA5(YGL169W)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SUI2(YJR007W)|FD-Score:-5|P-value:2.93E-7|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF10(YDR167W)|FD-Score:3.8|P-value:7.35E-5|Clearance:0.22||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF6(YGL112C)|FD-Score:3.47|P-value:2.64E-4|Clearance:0.16||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TIF35(YDR429C)|FD-Score:-5.66|P-value:7.78E-9|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP6(YDR449C)|FD-Score:-5.22|P-value:8.75E-8|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP8(YGR128C)|FD-Score:-3.73|P-value:9.47E-5|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR190W(YBR190W_d)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YJL086C(YJL086C_d)|FD-Score:5.87|P-value:2.20E-9|Clearance:0.68||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:-3.81|P-value:6.95E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPR136C(YPR136C_d)|FD-Score:-5.66|P-value:7.46E-9|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ACP1(YKL192C)|FD-Score:3.83|P-value:6.41E-5|Clearance:0.03||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:ACT1(YFL039C)|FD-Score:-3.2|P-value:6.97E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT3(YJL014W)|FD-Score:-3.1|P-value:9.72E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDS1(YBR029C)|FD-Score:-3.19|P-value:7.11E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:ENP1(YBR247C)|FD-Score:-4.28|P-value:9.52E-6|Clearance:0||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:HEM12(YDR047W)|FD-Score:5.36|P-value:4.09E-8|Clearance:0.68||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MCM7(YBR202W)|FD-Score:-4.04|P-value:2.70E-5|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NOG1(YPL093W)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP1(YDL014W)|FD-Score:-3.14|P-value:8.56E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NSL1(YPL233W)|FD-Score:3.3|P-value:4.78E-4|Clearance:0.24||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:PTR3(YFR029W)|FD-Score:4.15|P-value:1.63E-5|Clearance:0.32||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RAT1(YOR048C)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RLP24(YLR009W)|FD-Score:-4.19|P-value:1.39E-5|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPL10(YLR075W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL18A(YOL120C)|FD-Score:-4.95|P-value:3.70E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL25(YOL127W)|FD-Score:-5.25|P-value:7.77E-8|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL5(YPL131W)|FD-Score:-5.51|P-value:1.76E-8|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPO31(YOR116C)|FD-Score:4.62|P-value:1.89E-6|Clearance:0.47||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:-4.09|P-value:2.20E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPR2(YIR015W)|FD-Score:4.69|P-value:1.40E-6|Clearance:0.06||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPS2(YGL123W)|FD-Score:-5.47|P-value:2.30E-8|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.12|P-value:9.14E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:-7.22|P-value:2.64E-13|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC1(YDR164C)|FD-Score:-3.09|P-value:9.92E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SSL1(YLR005W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.11||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SUA5(YGL169W)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:SUI2(YJR007W)|FD-Score:-5|P-value:2.93E-7|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF10(YDR167W)|FD-Score:3.8|P-value:7.35E-5|Clearance:0.22||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TAF6(YGL112C)|FD-Score:3.47|P-value:2.64E-4|Clearance:0.16||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TIF35(YDR429C)|FD-Score:-5.66|P-value:7.78E-9|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UTP6(YDR449C)|FD-Score:-5.22|P-value:8.75E-8|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP8(YGR128C)|FD-Score:-3.73|P-value:9.47E-5|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR190W(YBR190W_d)|FD-Score:-3.48|P-value:2.48E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YJL086C(YJL086C_d)|FD-Score:5.87|P-value:2.20E-9|Clearance:0.68||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:-3.81|P-value:6.95E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPR136C(YPR136C_d)|FD-Score:-5.66|P-value:7.46E-9|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 738374
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ATG5(YPL149W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BRR1(YPR057W)|FD-Score:-5.41|P-value:3.22E-8||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CIT1(YNR001C)|FD-Score:6.47|P-value:5.02E-11||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:6.38|P-value:8.68E-11||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS10(YNR075W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DUG2(YBR281C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:END3(YNL084C)|FD-Score:6.24|P-value:2.25E-10||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERC1(YHR032W)|FD-Score:-3.89|P-value:4.91E-5||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:ERV29(YGR284C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FEN2(YCR028C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FSH1(YHR049W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GDB1(YPR184W)|FD-Score:5.67|P-value:7.24E-9||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GID7(YCL039W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GIM4(YEL003W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GLY1(YEL046C)|FD-Score:-5.52|P-value:1.67E-8||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GNP1(YDR508C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GSY2(YLR258W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ISC10(YER180C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MRPL31(YKL138C)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAS2(YIL007C)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:NCR1(YPL006W)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:OM45(YIL136W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:PDA1(YER178W)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PFK1(YGR240C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:RAD5(YLR032W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RCN1(YKL159C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:RCY1(YJL204C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RFM1(YOR279C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIP1(YEL024W)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:ROM2(YLR371W)|FD-Score:5.92|P-value:1.63E-9||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL11B(YGR085C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL36B(YPL249C-A)|FD-Score:-3.77|P-value:8.07E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS29A(YLR388W)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SAS4(YDR181C)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SEE1(YIL064W)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SHC1(YER096W)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIN4(YNL236W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SNF4(YGL115W)|FD-Score:5.43|P-value:2.82E-8||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SPH1(YLR313C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SSP1(YHR184W)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis Gene:STE24(YJR117W)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:TAN1(YGL232W)|FD-Score:-3.87|P-value:5.35E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TMA20(YER007C-A)|FD-Score:-4.09|P-value:2.15E-5||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL062W(YBL062W_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR016W(YCR016W_p)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR056C(YDR056C_p)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YEL045C(YEL045C_d)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGL260W(YGL260W_p)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR273C(YGR273C_p)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YGR290W(YGR290W_d)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YLR126C(YLR126C_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YLR326W(YLR326W_p)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YME2(YMR302C)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YNL057W(YNL057W_d)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR025C(YNR025C_d)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR385W(YOR385W_p)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:AAT2(YLR027C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ATG5(YPL149W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BRR1(YPR057W)|FD-Score:-5.41|P-value:3.22E-8||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CIT1(YNR001C)|FD-Score:6.47|P-value:5.02E-11||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:6.38|P-value:8.68E-11||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS10(YNR075W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DUG2(YBR281C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:END3(YNL084C)|FD-Score:6.24|P-value:2.25E-10||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERC1(YHR032W)|FD-Score:-3.89|P-value:4.91E-5||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:ERV29(YGR284C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FEN2(YCR028C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FSH1(YHR049W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GDB1(YPR184W)|FD-Score:5.67|P-value:7.24E-9||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GID7(YCL039W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions Gene:GIM4(YEL003W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GLY1(YEL046C)|FD-Score:-5.52|P-value:1.67E-8||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GNP1(YDR508C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GSY2(YLR258W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ISC10(YER180C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MRPL31(YKL138C)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAS2(YIL007C)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:NCR1(YPL006W)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:OM45(YIL136W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:PDA1(YER178W)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PFK1(YGR240C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:RAD5(YLR032W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RCN1(YKL159C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:RCY1(YJL204C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RFM1(YOR279C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIP1(YEL024W)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:ROM2(YLR371W)|FD-Score:5.92|P-value:1.63E-9||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL11B(YGR085C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL36B(YPL249C-A)|FD-Score:-3.77|P-value:8.07E-5||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS29A(YLR388W)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SAS4(YDR181C)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SEE1(YIL064W)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SHC1(YER096W)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIN4(YNL236W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SNF4(YGL115W)|FD-Score:5.43|P-value:2.82E-8||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SPH1(YLR313C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SSP1(YHR184W)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis Gene:STE24(YJR117W)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:TAN1(YGL232W)|FD-Score:-3.87|P-value:5.35E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TMA20(YER007C-A)|FD-Score:-4.09|P-value:2.15E-5||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL062W(YBL062W_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR016W(YCR016W_p)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR056C(YDR056C_p)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YEL045C(YEL045C_d)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGL260W(YGL260W_p)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium Gene:YGR273C(YGR273C_p)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YGR290W(YGR290W_d)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YLR126C(YLR126C_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YLR326W(YLR326W_p)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YME2(YMR302C)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YNL057W(YNL057W_d)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR025C(YNR025C_d)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR385W(YOR385W_p)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL086C_d5.872.20E-90.68YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YDR047W5.364.09E-80.68HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YIR015W4.691.40E-60.06RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YOR116C4.621.89E-60.47RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YFR029W4.151.63E-50.32PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YKL192C3.836.41E-50.03ACP1Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
YDR167W3.807.35E-50.22TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YLR005W3.571.75E-40.11SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YGL112C3.472.64E-40.16TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YPL233W3.304.78E-40.24NSL1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YGR264C3.070.001080.10MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YLR175W2.970.001510.01CBF5Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
YPL007C2.950.001570.06TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YKL196C2.890.001920.14YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YLR277C2.750.002960.07YSH1Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNR001C6.475.02E-11CIT1Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
YGR167W6.388.68E-11CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YNL084C6.242.25E-10END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YLR371W5.921.63E-9ROM2GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p
YPR184W5.677.24E-9GDB1Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress
YGL115W5.432.82E-8SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YER096W4.993.06E-7SHC1Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication
YNL041C4.671.53E-6COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YPL006W4.661.61E-6NCR1Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein
YGR085C4.592.22E-6RPL11BRibosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication
YOR385W_p4.562.57E-6YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YDR056C_p4.523.10E-6YDR056C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein
YJR117W4.483.65E-6STE24Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
YER178W4.376.25E-6PDA1E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose
YBL029C-A_p4.261.01E-5YBL029C-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica

GO enrichment analysis for SGTC_2179
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1719.84E-40SGTC_2739tacrine 100.9 μMMiscellaneous19350.156863heme requiring
0.1584.61E-34SGTC_2645abamectin 100.0 μMMicrosource (Natural product library)57022080.023622heme requiring
0.1554.83E-33SGTC_490phenamil 164.0 μMMiscellaneous47550.131148heme requiring
0.1432.18E-28SGTC_21826325192 200.0 μMChembridge (Fragment library)7362480.107143Golgi
0.1397.77E-27SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.0843373RNA pol III & RNase P/MRP
0.1373.39E-26SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0925926heme requiring
0.1331.61E-24SGTC_30329089427 49.5 μMChembridge (Drug-like library)171737070.067567660S ribosome export
0.1323.68E-24SGTC_12690831-0796 3.0 μMChemDiv (Drug-like library)9277580.15254260S ribosome export
0.1271.20E-22SGTC_14043909-7820 48.3 μMChemDiv (Drug-like library)237413730.088235360S ribosome export
0.1262.68E-22SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.0740741superoxide
0.1232.51E-21SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.069444460S ribosome export
0.1193.35E-20SGTC_32499135183 49.5 μMChembridge (Drug-like library)49027790.10526360S ribosome export
0.1195.01E-20SGTC_1619st003326 88.7 μMTimTec (Natural product derivative library)28490320.10344860S ribosome export
0.1172.01E-19SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.137931heme biosynthesis & mitochondrial translocase
0.1156.63E-19SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.13559360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13321480-065317.5 μM0.3061225749438ChemDiv (Drug-like library)219.259662.79503TSC3-RPN4
SGTC_2701st07776714.72 μM0.2941185721188TimTec (Natural product derivative library)281.329044.46903
SGTC_521r062-000513.8 μM0.2857142832033ChemDiv (Drug-like library)233.653723.1104
SGTC_970535-070119.13 μM0.2372882832013ChemDiv (Drug-like library)311.381543.2440660S ribosome export
SGTC_12480469-068758.6 μM0.2363644017482ChemDiv (Drug-like library)284.223722.88805
SGTC_1693xanthone101.9 μM0.2257020TimTec (Natural product derivative library)196.201422.96402DNA damage response
SGTC_2532xanthone78.9 μM0.2257020Microsource (Natural product library)196.201422.96402
SGTC_1527coumarin137 μM0.209302323TimTec (Pure natural product library)146.142741.89902
SGTC_13571503-130339.5 μM0.207547292895ChemDiv (Drug-like library)239.313962.7104
SGTC_1655st01254655.1 μM0.207547720985TimTec (Natural product derivative library)237.253342.63113
SGTC_2262901488567.46 μM0.2075476457424Chembridge (Fragment library)213.238542.07115