6301957

methyl 2-anilino-4-oxothiophene-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2180
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 845462
SMILES COC(=O)C1=C(SCC1=O)NC2=CC=CC=C2
Standardized SMILES COC(=O)C1=C(Nc2ccccc2)SCC1=O
Molecular weight 249.2856
ALogP 2.09
H-bond donor count 1
H-bond acceptor count 5
Response signature RSC complex & mRNA processing

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.26
% growth inhibition (Hom. pool) 4.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 845462
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.13|P-value:1.84E-5|Clearance:0.21||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ALG1(YBR110W)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.04||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:ARP4(YJL081C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.16||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:4.99|P-value:2.95E-7|Clearance:0.41||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRR6(YGL247W)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CRM1(YGR218W)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.13||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:GLN4(YOR168W)|FD-Score:4.59|P-value:2.24E-6|Clearance:0.2||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:GNA1(YFL017C)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.09||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:NIP7(YPL211W)|FD-Score:-3.24|P-value:5.88E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NOP2(YNL061W)|FD-Score:-3.6|P-value:1.57E-4|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RER2(YBR002C)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.36||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RHO1(YPR165W)|FD-Score:10.7|P-value:3.42E-27|Clearance:4.37||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB7(YDR404C)|FD-Score:5.87|P-value:2.15E-9|Clearance:0.88||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RSC58(YLR033W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:6.29|P-value:1.55E-10|Clearance:0.42||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SFH1(YLR321C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SPC105(YGL093W)|FD-Score:4.38|P-value:5.82E-6|Clearance:0.03||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSL1(YLR005W)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.05||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TFB1(YDR311W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TFC3(YAL001C)|FD-Score:6.37|P-value:9.71E-11|Clearance:0.07||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIF35(YDR429C)|FD-Score:-3.5|P-value:2.32E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YLR230W(YLR230W_d)|FD-Score:-3.83|P-value:6.40E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YTH1(YPR107C)|FD-Score:4.35|P-value:6.79E-6|Clearance:0.14||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:4.13|P-value:1.84E-5|Clearance:0.21||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ALG1(YBR110W)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.04||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:ARP4(YJL081C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.16||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:4.99|P-value:2.95E-7|Clearance:0.41||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRR6(YGL247W)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CRM1(YGR218W)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.13||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:GLN4(YOR168W)|FD-Score:4.59|P-value:2.24E-6|Clearance:0.2||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:GNA1(YFL017C)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.09||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:NIP7(YPL211W)|FD-Score:-3.24|P-value:5.88E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:NOP2(YNL061W)|FD-Score:-3.6|P-value:1.57E-4|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RER2(YBR002C)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.36||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RHO1(YPR165W)|FD-Score:10.7|P-value:3.42E-27|Clearance:4.37||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB7(YDR404C)|FD-Score:5.87|P-value:2.15E-9|Clearance:0.88||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RSC58(YLR033W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:6.29|P-value:1.55E-10|Clearance:0.42||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SFH1(YLR321C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SPC105(YGL093W)|FD-Score:4.38|P-value:5.82E-6|Clearance:0.03||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSL1(YLR005W)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.05||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TFB1(YDR311W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TFC3(YAL001C)|FD-Score:6.37|P-value:9.71E-11|Clearance:0.07||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIF35(YDR429C)|FD-Score:-3.5|P-value:2.32E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YLR230W(YLR230W_d)|FD-Score:-3.83|P-value:6.40E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YTH1(YPR107C)|FD-Score:4.35|P-value:6.79E-6|Clearance:0.14||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 845462
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ASF1(YJL115W)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:AVT1(YJR001W)|FD-Score:3.82|P-value:6.80E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCK2(YER167W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CAN1(YEL063C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CSF1(YLR087C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DMA1(YHR115C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:GAL80(YML051W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:GNP1(YDR508C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HIM1(YDR317W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HSP30(YCR021C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:HUA1(YGR268C)|FD-Score:3.74|P-value:9.15E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IAH1(YOR126C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IRC14(YOR135C_d)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:MRP2(YPR166C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS51(YLR203C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NAS6(YGR232W)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:OCT1(YKL134C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PAU4(YLR461W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PTH2(YBL057C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUF2(YPR042C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RGS2(YOR107W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RPS9B(YBR189W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RTT109(YLL002W)|FD-Score:-4.37|P-value:6.18E-6||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:STE20(YHL007C)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:STE50(YCL032W)|FD-Score:-3.15|P-value:8.29E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUL2(YLR092W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:THI3(YDL080C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TRP1(YDR007W)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VBA5(YKR105C_p)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:YBL053W(YBL053W_d)|FD-Score:-3.89|P-value:4.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR064W(YBR064W_d)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YIP5(YGL161C)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YPR117W(YPR117W_p)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Putative protein of unknown function Gene:ADE1(YAR015W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ASF1(YJL115W)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:AVT1(YJR001W)|FD-Score:3.82|P-value:6.80E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCK2(YER167W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CAN1(YEL063C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CSF1(YLR087C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DMA1(YHR115C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:GAL80(YML051W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:GNP1(YDR508C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:HIM1(YDR317W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HSP30(YCR021C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:HUA1(YGR268C)|FD-Score:3.74|P-value:9.15E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IAH1(YOR126C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IRC14(YOR135C_d)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:MRP2(YPR166C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS51(YLR203C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NAS6(YGR232W)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:OCT1(YKL134C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PAU4(YLR461W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PTH2(YBL057C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUF2(YPR042C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RGS2(YOR107W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RPS9B(YBR189W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RTT109(YLL002W)|FD-Score:-4.37|P-value:6.18E-6||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:STE20(YHL007C)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:STE50(YCL032W)|FD-Score:-3.15|P-value:8.29E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUL2(YLR092W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:THI3(YDL080C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TRP1(YDR007W)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VBA5(YKR105C_p)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:YBL053W(YBL053W_d)|FD-Score:-3.89|P-value:4.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR064W(YBR064W_d)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YIP5(YGL161C)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YPR117W(YPR117W_p)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR165W10.703.42E-274.37RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YAL001C6.379.71E-110.07TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YFR037C6.291.55E-100.42RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDR404C5.872.15E-90.88RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YMR033W4.992.95E-70.41ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOR168W4.592.24E-60.20GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YGL093W4.385.82E-60.03SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YPR107C4.356.79E-60.14YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YFL017C4.211.26E-50.09GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YNR016C4.131.84E-50.21ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YBR002C3.924.44E-50.36RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YJL081C3.561.85E-40.16ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YLR321C3.403.39E-40.09SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YLR005W3.314.70E-40.05SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YGR218W3.255.68E-40.13CRM1Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER167W4.651.69E-6BCK2Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations
YDR007W4.582.37E-6TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YCR021C4.572.47E-6HSP30Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
YDR508C4.092.20E-5GNP1High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication
YPR117W_p4.082.25E-5YPR117W_pPutative protein of unknown function
YLR461W4.052.54E-5PAU4Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
YHL007C3.953.98E-5STE20Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p
YOR135C_d3.934.27E-5IRC14_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
YJR001W3.826.80E-5AVT1Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YGL161C3.807.32E-5YIP5Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
YGR268C3.749.15E-5HUA1Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
YOR107W3.711.04E-4RGS2Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
YPR042C3.502.33E-4PUF2Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
YDR317W3.482.50E-4HIM1Protein of unknown function involved in DNA repair
YBL057C3.373.70E-4PTH2One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth

GO enrichment analysis for SGTC_2180
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2947.43E-118SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.134328RSC complex & mRNA processing
0.2902.97E-114SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.183099cell wall
0.2661.10E-95SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.121212RSC complex & mRNA processing
0.2585.65E-90SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.136364RSC complex & mRNA processing
0.2553.13E-88SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0882353RSC complex & mRNA processing
0.2488.04E-83SGTC_5860957-0265 8.2 μMChemDiv (Drug-like library)67523090.0823529cell wall
0.2452.88E-81SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.12963RSC complex & mRNA processing
0.2345.02E-74SGTC_7093807-1509 243.0 μMChemDiv (Drug-like library)18866770.0277778RSC complex & mRNA processing
0.2299.41E-71SGTC_14934348-0038 111.0 μMChemDiv (Drug-like library)8004240.0923077RSC complex & mRNA processing
0.2178.35E-64SGTC_33229139058 2.1 μMChembridge (Drug-like library)49089260.103896cell wall
0.2151.31E-62SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0819672
0.2124.58E-61SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0869565RSC & ERG11
0.2111.52E-60SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.196078RSC complex & mRNA processing
0.2082.94E-58SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.118644RSC complex & mRNA processing
0.2021.69E-55SGTC_6731082-0474 110.0 μMChemDiv (Drug-like library)30980460.166667RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6173770-009835 μM0.4727275962639ChemDiv (Drug-like library)327.354423.38616exosome
SGTC_3232913287449.47 μM0.3753784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_10234482-388816 μM0.3584913303168ChemDiv (Drug-like library)267.324062.58205TSC3-RPN4
SGTC_6704204-0025119 μM0.339286719039ChemDiv (Drug-like library)341.745183.07115copper-dependent oxidative stress
SGTC_20645220758200 μM0.333333788518Chembridge (Fragment library)263.312221.92615
SGTC_1167k781-0610148 μM0.3214294525169ChemDiv (Drug-like library)276.314282.7934RNA processing & uracil transport
SGTC_23157733312200 μM0.313726726316Chembridge (Fragment library)210.25291.93523
SGTC_23175986382200 μM0.3125877883Chembridge (Fragment library)229.27442.07913
SGTC_5544483-2211187 μM0.296296747107ChemDiv (Drug-like library)285.29462.45314
SGTC_12550566-0045221 μM0.2962962788469ChemDiv (Drug-like library)304.728323.04223excess fatty acid