6316240

2-(hydroxymethyl)-4-phenylphenol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2181
Screen concentration 54.3 μM
Source Chembridge (Fragment library)
PubChem CID 12779990
SMILES C1=CC=C(C=C1)C2=CC(=C(C=C2)O)CO
Standardized SMILES OCc1cc(ccc1O)c2ccccc2
Molecular weight 200.2332
ALogP 2.5
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.79
% growth inhibition (Hom. pool) 3.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 12779990
Download HIP data (tab-delimited text)  (excel)
Gene:ERG12(YMR208W)|FD-Score:4.01|P-value:3.10E-5|Clearance:0.83||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:NOP4(YPL043W)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.06||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:POL3(YDL102W)|FD-Score:-4.23|P-value:1.15E-5|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRI2(YKL045W)|FD-Score:4.29|P-value:8.81E-6|Clearance:0.83||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:QNS1(YHR074W)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.05||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RRP43(YCR035C)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SEC10(YLR166C)|FD-Score:3.18|P-value:7.44E-4|Clearance:0.01||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:TFB1(YDR311W)|FD-Score:-4.49|P-value:3.58E-6|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TFC8(YPL007C)|FD-Score:-3.31|P-value:4.64E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:YDR053W(YDR053W_d)|FD-Score:-3.45|P-value:2.81E-4|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YLR230W(YLR230W_d)|FD-Score:-3.32|P-value:4.48E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YPR136C(YPR136C_d)|FD-Score:4.87|P-value:5.49E-7|Clearance:0.83||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ERG12(YMR208W)|FD-Score:4.01|P-value:3.10E-5|Clearance:0.83||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:NOP4(YPL043W)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.06||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:POL3(YDL102W)|FD-Score:-4.23|P-value:1.15E-5|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRI2(YKL045W)|FD-Score:4.29|P-value:8.81E-6|Clearance:0.83||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:QNS1(YHR074W)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.05||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RRP43(YCR035C)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SEC10(YLR166C)|FD-Score:3.18|P-value:7.44E-4|Clearance:0.01||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:TFB1(YDR311W)|FD-Score:-4.49|P-value:3.58E-6|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TFC8(YPL007C)|FD-Score:-3.31|P-value:4.64E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:YDR053W(YDR053W_d)|FD-Score:-3.45|P-value:2.81E-4|Clearance:0||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YLR230W(YLR230W_d)|FD-Score:-3.32|P-value:4.48E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YPR136C(YPR136C_d)|FD-Score:4.87|P-value:5.49E-7|Clearance:0.83||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 12779990
Download HOP data (tab-delimited text)  (excel)
Gene:AFG3(YER017C)|FD-Score:5.78|P-value:3.72E-9||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ARR1(YPR199C)|FD-Score:-9.01|P-value:1.07E-19||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATG15(YCR068W)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATG22(YCL038C)|FD-Score:4.91|P-value:4.44E-7||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:AVT3(YKL146W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCK2(YER167W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BSC6(YOL137W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CCE1(YKL011C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CHS3(YBR023C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CHS7(YHR142W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CIT1(YNR001C)|FD-Score:4.31|P-value:8.24E-6||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CLU1(YMR012W)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:CSF1(YLR087C)|FD-Score:6.34|P-value:1.16E-10||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTK3(YML112W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DMC1(YER179W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:ESC1(YMR219W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FEN1(YCR034W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:GEA2(YEL022W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HSV2(YGR223C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:IRC2(YDR112W_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC7(YFR055W_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:MPD2(YOL088C)|FD-Score:4.87|P-value:5.50E-7||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPL11(YDL202W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTF2(YDL044C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NUP42(YDR192C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:PDR16(YNL231C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PHO84(YML123C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO88(YBR106W)|FD-Score:8.24|P-value:8.30E-17||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RAD5(YLR032W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RDL1(YOR285W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIM9(YMR063W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL1B(YGL135W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL6A(YML073C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:6.8|P-value:5.20E-12||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM28(YDR494W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SAE2(YGL175C)|FD-Score:4.29|P-value:9.04E-6||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAS4(YDR181C)|FD-Score:5.42|P-value:3.01E-8||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SEM1(YDR363W-A)|FD-Score:4.17|P-value:1.56E-5||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SFC1(YJR095W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SHP1(YBL058W)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SNG1(YGR197C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:SNT2(YGL131C)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SPO11(YHL022C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:TRP1(YDR007W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP14(YBR058C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UBP5(YER144C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VMA1(YDL185W)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YCL012C(YCL012C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YER156C(YER156C_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YFR032C-B(YFR032C-B_p)|FD-Score:4.79|P-value:8.25E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL034C(YGL034C_d)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR064W(YGR064W_d)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL058W(YIL058W_d)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL161W(YIL161W_p)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YJL160C(YJL160C_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR084W(YJR084W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YJR096W(YJR096W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YMR087W(YMR087W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR31(YFR049W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YND1(YER005W)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL170W(YNL170W_d)|FD-Score:4.89|P-value:5.05E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR139C(YOR139C_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPR172W(YPR172W_p)|FD-Score:6.25|P-value:2.01E-10||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:ZEO1(YOL109W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:AFG3(YER017C)|FD-Score:5.78|P-value:3.72E-9||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:ARR1(YPR199C)|FD-Score:-9.01|P-value:1.07E-19||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATG15(YCR068W)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATG22(YCL038C)|FD-Score:4.91|P-value:4.44E-7||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:AVT3(YKL146W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCK2(YER167W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BSC6(YOL137W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CCE1(YKL011C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CHS3(YBR023C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CHS7(YHR142W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CIT1(YNR001C)|FD-Score:4.31|P-value:8.24E-6||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CLU1(YMR012W)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:CSF1(YLR087C)|FD-Score:6.34|P-value:1.16E-10||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTK3(YML112W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DMC1(YER179W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:ESC1(YMR219W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FEN1(YCR034W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:GEA2(YEL022W)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HSV2(YGR223C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:IRC2(YDR112W_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC7(YFR055W_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:MPD2(YOL088C)|FD-Score:4.87|P-value:5.50E-7||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPL11(YDL202W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTF2(YDL044C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NUP42(YDR192C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:PDR16(YNL231C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PHO84(YML123C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO88(YBR106W)|FD-Score:8.24|P-value:8.30E-17||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RAD5(YLR032W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RDL1(YOR285W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIM9(YMR063W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL1B(YGL135W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL6A(YML073C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:6.8|P-value:5.20E-12||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM28(YDR494W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SAE2(YGL175C)|FD-Score:4.29|P-value:9.04E-6||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAS4(YDR181C)|FD-Score:5.42|P-value:3.01E-8||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SEM1(YDR363W-A)|FD-Score:4.17|P-value:1.56E-5||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SFC1(YJR095W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SHP1(YBL058W)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SNG1(YGR197C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:SNT2(YGL131C)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SPO11(YHL022C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:TRP1(YDR007W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.85|P-value:5.99E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP14(YBR058C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UBP5(YER144C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VMA1(YDL185W)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YCL012C(YCL012C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YER156C(YER156C_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YFR032C-B(YFR032C-B_p)|FD-Score:4.79|P-value:8.25E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL034C(YGL034C_d)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR064W(YGR064W_d)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL058W(YIL058W_d)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL161W(YIL161W_p)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YJL160C(YJL160C_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR084W(YJR084W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YJR096W(YJR096W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YMR087W(YMR087W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR31(YFR049W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YND1(YER005W)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL170W(YNL170W_d)|FD-Score:4.89|P-value:5.05E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR139C(YOR139C_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPR172W(YPR172W_p)|FD-Score:6.25|P-value:2.01E-10||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:ZEO1(YOL109W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR136C_d4.875.49E-70.83YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YKL045W4.298.81E-60.83PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YMR208W4.013.10E-50.83ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YLR166C3.187.44E-40.01SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YPL043W3.177.69E-40.06NOP4Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
YCR035C3.119.29E-40.01RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YHR074W3.109.66E-40.05QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YNR043W3.050.001160.04MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YGL044C3.010.001310.02RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YER048W-A2.990.001380.08ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YJR112W2.910.001800.09NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YJL011C2.820.002380.04RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YFR037C2.780.002710.06RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YPL218W2.720.003250.04SAR1GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport
YPL142C_d2.680.003690.03YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR106W8.248.30E-17PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YDL020C6.805.20E-12RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLR087C6.341.16E-10CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPR172W_p6.252.01E-10YPR172W_pPutative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication
YER017C5.783.72E-9AFG3Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging
YDR181C5.423.01E-8SAS4Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
YKL211C5.111.58E-7TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YCL038C4.914.44E-7ATG22Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation
YNL170W_d4.895.05E-7YNL170W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOL088C4.875.50E-7MPD2Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
YFR032C-B_p4.798.25E-7YFR032C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YER005W4.731.14E-6YND1Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity
YFR049W4.612.02E-6YMR31Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36
YDL185W4.562.59E-6VMA1Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
YNR001C4.318.24E-6CIT1Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2181
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2041.79E-56SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.160714
0.1673.24E-38SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.106383
0.1361.30E-25SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.118644
0.1322.27E-24SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.177778
0.1301.05E-23SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0454545
0.1292.17E-23SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.0985916plasma membrane duress
0.1292.36E-23SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.0625
0.1287.50E-23SGTC_1761486-1328 72.8 μMChemDiv (Drug-like library)223102310.0694444
0.1262.41E-22SGTC_2051486-1330 71.9 μMChemDiv (Drug-like library)41188340.0615385
0.1263.26E-22SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0405405
0.1254.41E-22SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.0847458
0.1181.05E-19SGTC_22997976368 114.9 μMChembridge (Fragment library)29797180.170213
0.1163.50E-19SGTC_450aminopyrene 156.0 nMMiscellaneous153520.1
0.1163.87E-19SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.0606061
0.1141.76E-18SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.153846TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2723salbutamol83.58 μM0.3571439884233NIH Clinical Collection337.389220.33468
SGTC_2881315-001935.1 μM0.3478266811474ChemDiv (Drug-like library)541.120963.9423RSC & ERG11
SGTC_1064honokiol6.76 μM0.34146372303NIH Clinical Collection266.334324.8822
SGTC_550767-02856.56 μM0.31253116166ChemDiv (Drug-like library)325.446086.93500
SGTC_2588indole-3-carbinol100 μM0.3055563712Microsource (Natural product library)147.173861.51821
SGTC_275trichlorophene5.69 μM0.29729762616Miscellaneous409.69037.04633mitochondrial stress
SGTC_3451362-0200395 μM0.2954551419823ChemDiv (Drug-like library)264.384684.45100
SGTC_1474466-053829.23 μM0.2926836742687ChemDiv (Drug-like library)236.268581.34823ergosterol biosynthesis
SGTC_1162k048-013020.1 μM0.2888896797406ChemDiv (Drug-like library)431.120624.52312Golgi
SGTC_23217390090138.78 μM0.288889649046Chembridge (Fragment library)214.263062.85423