6325192

N-(4-aminophenyl)-3-methylbenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2182
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 736248
SMILES CC1=CC=CC(=C1)C(=O)NC2=CC=C(C=C2)N
Standardized SMILES Cc1cccc(c1)C(=O)Nc2ccc(N)cc2
Molecular weight 226.2738
ALogP 2.35
H-bond donor count 2
H-bond acceptor count 2
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.39
% growth inhibition (Hom. pool) 7.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 736248
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:MCM7(YBR202W)|FD-Score:-3.31|P-value:4.71E-4|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NNF1(YJR112W)|FD-Score:4.86|P-value:5.78E-7|Clearance:1.13||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:POP7(YBR167C)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPO26(YPR187W)|FD-Score:-5.13|P-value:1.41E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:-4.11|P-value:2.01E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS5(YJR123W)|FD-Score:-3.16|P-value:7.79E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RSC8(YFR037C)|FD-Score:-3.18|P-value:7.36E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC63(YOR254C)|FD-Score:3.13|P-value:8.88E-4|Clearance:0.01||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPC25(YER018C)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.33||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TFB1(YDR311W)|FD-Score:-4.39|P-value:5.67E-6|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:ULP2(YIL031W)|FD-Score:-3.27|P-value:5.44E-4|Clearance:0||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:UTP4(YDR324C)|FD-Score:3.73|P-value:9.46E-5|Clearance:0.28||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:APC5(YOR249C)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:MCM7(YBR202W)|FD-Score:-3.31|P-value:4.71E-4|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NNF1(YJR112W)|FD-Score:4.86|P-value:5.78E-7|Clearance:1.13||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:POP7(YBR167C)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.12||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPO26(YPR187W)|FD-Score:-5.13|P-value:1.41E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:-4.11|P-value:2.01E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS5(YJR123W)|FD-Score:-3.16|P-value:7.79E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RSC8(YFR037C)|FD-Score:-3.18|P-value:7.36E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC63(YOR254C)|FD-Score:3.13|P-value:8.88E-4|Clearance:0.01||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPC25(YER018C)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.33||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TFB1(YDR311W)|FD-Score:-4.39|P-value:5.67E-6|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:ULP2(YIL031W)|FD-Score:-3.27|P-value:5.44E-4|Clearance:0||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:UTP4(YDR324C)|FD-Score:3.73|P-value:9.46E-5|Clearance:0.28||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 736248
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:4.34|P-value:7.03E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARL1(YBR164C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG22(YCL038C)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:BBC1(YJL020C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BRR1(YPR057W)|FD-Score:-4.77|P-value:9.41E-7||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CLC1(YGR167W)|FD-Score:7.94|P-value:9.92E-16||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNM67(YNL225C)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:4.84|P-value:6.50E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPD1(YGR247W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CSH1(YBR161W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:ECM18(YDR125C)|FD-Score:-4.43|P-value:4.66E-6||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FUN26(YAL022C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GNP1(YDR508C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HSP30(YCR021C)|FD-Score:4.76|P-value:9.50E-7||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:IAH1(YOR126C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IRC21(YMR073C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRS4(YKR019C)|FD-Score:5.29|P-value:6.03E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LOA1(YPR139C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LSM12(YHR121W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LTP1(YPR073C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MEC3(YLR288C)|FD-Score:6.05|P-value:7.41E-10||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MTC1(YJL123C)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:PDC5(YLR134W)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PUF2(YPR042C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:PUS2(YGL063W)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RCY1(YJL204C)|FD-Score:6.39|P-value:8.55E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RPN4(YDL020C)|FD-Score:5.23|P-value:8.45E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTA1(YGR213C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SPH1(YLR313C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SSN3(YPL042C)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SYS1(YJL004C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TLG2(YOL018C)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UGX2(YDL169C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:VAC14(YLR386W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPH1(YOR270C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:YER076C(YER076C_p)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YJR096W(YJR096W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:AFT1(YGL071W)|FD-Score:4.34|P-value:7.03E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARL1(YBR164C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATG22(YCL038C)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:BBC1(YJL020C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches Gene:BRR1(YPR057W)|FD-Score:-4.77|P-value:9.41E-7||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CLC1(YGR167W)|FD-Score:7.94|P-value:9.92E-16||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNM67(YNL225C)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:4.84|P-value:6.50E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPD1(YGR247W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CSH1(YBR161W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:ECM18(YDR125C)|FD-Score:-4.43|P-value:4.66E-6||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FUN26(YAL022C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GNP1(YDR508C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HSP30(YCR021C)|FD-Score:4.76|P-value:9.50E-7||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:IAH1(YOR126C)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:IRC21(YMR073C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRS4(YKR019C)|FD-Score:5.29|P-value:6.03E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LOA1(YPR139C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LSM12(YHR121W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LTP1(YPR073C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MEC3(YLR288C)|FD-Score:6.05|P-value:7.41E-10||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MTC1(YJL123C)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:PDC5(YLR134W)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PUF2(YPR042C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:PUS2(YGL063W)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RCY1(YJL204C)|FD-Score:6.39|P-value:8.55E-11||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RPN4(YDL020C)|FD-Score:5.23|P-value:8.45E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTA1(YGR213C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SPH1(YLR313C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SSN3(YPL042C)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SYS1(YJL004C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TLG2(YOL018C)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UGX2(YDL169C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:VAC14(YLR386W)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPH1(YOR270C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:YER076C(YER076C_p)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YJR096W(YJR096W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR112W4.865.78E-71.13NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YDR324C3.739.46E-50.28UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YER018C3.452.76E-40.33SPC25Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YOR254C3.138.88E-40.01SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YBR167C3.119.28E-40.12POP7Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YEL058W2.990.001380.12PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YLR008C2.870.002050.00PAM18Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
YEL055C2.870.002060.06POL5DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
YDL165W2.810.002460.01CDC36Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YNL114C_d2.800.002530.02YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YMR220W2.780.002720.10ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YNL113W2.680.003700.19RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YDR365C2.490.006430.02ESF1Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
YOR098C2.470.006710.01NUP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
YBR123C2.460.006960.05TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR167W7.949.92E-16CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YJL204C6.398.55E-11RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YLR288C6.057.41E-10MEC3DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
YKR019C5.296.03E-8IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YDL020C5.238.45E-8RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YNL041C4.846.50E-7COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YCR021C4.769.50E-7HSP30Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
YOL018C4.701.33E-6TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YGL071W4.347.03E-6AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YPR073C4.221.23E-5LTP1Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
YPR042C4.201.32E-5PUF2Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
YOR070C4.141.74E-5GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YOR126C4.072.31E-5IAH1Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing
YDR007W4.032.76E-5TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YCL038C3.885.30E-5ATG22Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation

GO enrichment analysis for SGTC_2182
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2101.11E-59SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.270833Golgi
0.1879.72E-48SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.166667Golgi
0.1851.26E-46SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0674847Golgi
0.1831.83E-45SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0677966Golgi
0.1776.41E-43SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.147059Golgi
0.1736.13E-41SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.0833333Golgi
0.1722.22E-40SGTC_29005328528 85.3 μMChembridge (Drug-like library)7953590.404255Golgi
0.1672.61E-38SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.0405405Golgi
0.1662.17E-37SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.114754PDR1
0.1647.13E-37SGTC_1078idebenone 36.1 μMMiscellaneous36860.0714286Golgi
0.1633.09E-36SGTC_24435493264 126.7 μMMiscellaneous53400550.0806452
0.1633.24E-36SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.224138Golgi
0.1625.16E-36SGTC_980074-0023 107.7 μMChemDiv (Drug-like library)35638210.145455Golgi
0.1557.14E-33SGTC_999SEC7.066 22.7 μMChemDiv (Drug-like library)7405110.0967742Golgi
0.1532.09E-32SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.169811Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20134024628200 μM0.755230760Chembridge (Fragment library)226.273762.35422
SGTC_2824799840451.95 μM0.585366837851Chembridge (Drug-like library)257.35073.64212
SGTC_21935925765200 μM0.5877370Chembridge (Fragment library)241.28512.49622
SGTC_3232913287449.47 μM0.476193784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_29087930301100 μM0.4615382223178Chembridge (Drug-like library)387.431143.00533
SGTC_2877904072471.43 μM0.4468096463895Chembridge (Drug-like library)285.337663.30813
SGTC_3070911700549.47 μM0.4468098779016Chembridge (Drug-like library)273.3021433.5313
SGTC_3004907612271.43 μM0.43137344782551Chembridge (Drug-like library)267.322383.53612
SGTC_3094911774249.47 μM0.43137317149698Chembridge (Drug-like library)312.3632.8523
SGTC_3225913081949.47 μM0.4313734547121Chembridge (Drug-like library)293.32322.66624amide catabolism