6113692

1-[4-(4-fluorophenyl)piperazin-1-yl]butan-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2185
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 874150
SMILES CCCC(=O)N1CCN(CC1)C2=CC=C(C=C2)F
Standardized SMILES CCCC(=O)N1CCN(CC1)c2ccc(F)cc2
Molecular weight 250.3119
ALogP 2.54
H-bond donor count 0
H-bond acceptor count 3
Response signature ergosterol biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.55
% growth inhibition (Hom. pool) 4.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 874150
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.06||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET4(YJL031C)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CBK1(YNL161W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.01||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CCT7(YJL111W)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC123(YLR215C)|FD-Score:5.49|P-value:1.98E-8|Clearance:0.33||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:DPB11(YJL090C)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.04||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:ERG11(YHR007C)|FD-Score:5.16|P-value:1.25E-7|Clearance:0.07||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:8.43|P-value:1.72E-17|Clearance:2.94||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:HSH49(YOR319W)|FD-Score:5.09|P-value:1.78E-7|Clearance:0.86||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSG1(YGL099W)|FD-Score:-3.58|P-value:1.74E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NOP8(YOL144W)|FD-Score:-3.56|P-value:1.85E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP57(YGR119C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PUP1(YOR157C)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.14||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:PWP1(YLR196W)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.08||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RAT1(YOR048C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.12||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:SEC10(YLR166C)|FD-Score:3.29|P-value:5.02E-4|Clearance:0.09||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEC23(YPR181C)|FD-Score:3.96|P-value:3.74E-5|Clearance:0.22||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SPP381(YBR152W)|FD-Score:3.29|P-value:4.93E-4|Clearance:0.01||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:YDR053W(YDR053W_d)|FD-Score:3.18|P-value:7.35E-4|Clearance:0.02||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YNL114C(YNL114C_d)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPL238C(YPL238C_d)|FD-Score:3.2|P-value:6.86E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ARP7(YPR034W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.06||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET4(YJL031C)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CBK1(YNL161W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.01||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CCT7(YJL111W)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC123(YLR215C)|FD-Score:5.49|P-value:1.98E-8|Clearance:0.33||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:DPB11(YJL090C)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.04||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:ERG11(YHR007C)|FD-Score:5.16|P-value:1.25E-7|Clearance:0.07||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:8.43|P-value:1.72E-17|Clearance:2.94||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:HSH49(YOR319W)|FD-Score:5.09|P-value:1.78E-7|Clearance:0.86||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSG1(YGL099W)|FD-Score:-3.58|P-value:1.74E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NOP8(YOL144W)|FD-Score:-3.56|P-value:1.85E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP57(YGR119C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PUP1(YOR157C)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.14||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:PWP1(YLR196W)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.08||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RAT1(YOR048C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.12||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:SEC10(YLR166C)|FD-Score:3.29|P-value:5.02E-4|Clearance:0.09||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEC23(YPR181C)|FD-Score:3.96|P-value:3.74E-5|Clearance:0.22||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SPP381(YBR152W)|FD-Score:3.29|P-value:4.93E-4|Clearance:0.01||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:YDR053W(YDR053W_d)|FD-Score:3.18|P-value:7.35E-4|Clearance:0.02||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YNL114C(YNL114C_d)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPL238C(YPL238C_d)|FD-Score:3.2|P-value:6.86E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 874150
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:-3.78|P-value:7.75E-5||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AIM36(YMR157C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ARL1(YBR164C)|FD-Score:-5.06|P-value:2.15E-7||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARP5(YNL059C)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BRR1(YPR057W)|FD-Score:-6.85|P-value:3.80E-12||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BSC5(YNR069C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CBP4(YGR174C)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:COG5(YNL051W)|FD-Score:-3.81|P-value:6.90E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS1(YNL336W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CST6(YIL036W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DCG1(YIR030C)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DFG16(YOR030W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DOC1(YGL240W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DOT6(YER088C)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:EDS1(YBR033W_p)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:ENV10(YLR065C)|FD-Score:-4.11|P-value:2.02E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:GDB1(YPR184W)|FD-Score:4.41|P-value:5.18E-6||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HMG1(YML075C)|FD-Score:6.49|P-value:4.26E-11||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOT13(YKL084W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HST1(YOL068C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUG1(YML058W-A)|FD-Score:3.87|P-value:5.36E-5||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IDH2(YOR136W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:ILM1(YJR118C)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:LSM12(YHR121W)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MUB1(YMR100W)|FD-Score:-3.91|P-value:4.59E-5||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NAP1(YKR048C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:PAT1(YCR077C)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PEP7(YDR323C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PTH2(YBL057C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RGI2(YIL057C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:RGP1(YDR137W)|FD-Score:-3.81|P-value:7.03E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.36|P-value:6.44E-6||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPN4(YDL020C)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRD1(YIL153W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RRT5(YFR032C_p)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RTS3(YGR161C_p)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SER33(YIL074C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SHP1(YBL058W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SIF2(YBR103W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SPO22(YIL073C)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SPS19(YNL202W)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SQS1(YNL224C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SRC1(YML034W)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:UBP5(YER144C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VAB2(YEL005C)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VPS27(YNR006W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS51(YKR020W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:-7.15|P-value:4.26E-13||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.4|P-value:5.51E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL066W(YAL066W_d)|FD-Score:-4.43|P-value:4.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR139W(YBR139W)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YBT1(YLL048C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCR102C(YCR102C_p)|FD-Score:-3.96|P-value:3.81E-5||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR056C(YDR056C_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YGR053C(YGR053C_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function Gene:YGR130C(YGR130C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YJL067W(YJL067W_d)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL182C(YJL182C_d)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YKL053W(YKL053W_d)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YNL057W(YNL057W_d)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR005C(YNR005C_d)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR053W(YOR053W_d)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPL114W(YPL114W_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR117W(YPR117W_p)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Putative protein of unknown function Gene:YPT6(YLR262C)|FD-Score:-6.66|P-value:1.35E-11||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ACM1(YPL267W)|FD-Score:-3.78|P-value:7.75E-5||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:AIM36(YMR157C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ARL1(YBR164C)|FD-Score:-5.06|P-value:2.15E-7||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARP5(YNL059C)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BRR1(YPR057W)|FD-Score:-6.85|P-value:3.80E-12||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BSC5(YNR069C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CBP4(YGR174C)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:COG5(YNL051W)|FD-Score:-3.81|P-value:6.90E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS1(YNL336W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CST6(YIL036W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DCG1(YIR030C)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DFG16(YOR030W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DOC1(YGL240W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DOT6(YER088C)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:EDS1(YBR033W_p)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:ENV10(YLR065C)|FD-Score:-4.11|P-value:2.02E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:GDB1(YPR184W)|FD-Score:4.41|P-value:5.18E-6||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HMG1(YML075C)|FD-Score:6.49|P-value:4.26E-11||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOT13(YKL084W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HST1(YOL068C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUG1(YML058W-A)|FD-Score:3.87|P-value:5.36E-5||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IDH2(YOR136W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:ILM1(YJR118C)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:LSM12(YHR121W)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MUB1(YMR100W)|FD-Score:-3.91|P-value:4.59E-5||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NAP1(YKR048C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:PAT1(YCR077C)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PEP7(YDR323C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PTH2(YBL057C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RGI2(YIL057C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:RGP1(YDR137W)|FD-Score:-3.81|P-value:7.03E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-4.36|P-value:6.44E-6||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPN4(YDL020C)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRD1(YIL153W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RRT5(YFR032C_p)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RTS3(YGR161C_p)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SER33(YIL074C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SHP1(YBL058W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SIF2(YBR103W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SPO22(YIL073C)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SPS19(YNL202W)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SQS1(YNL224C)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SRC1(YML034W)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:UBP5(YER144C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VAB2(YEL005C)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VPS27(YNR006W)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS51(YKR020W)|FD-Score:-3.17|P-value:7.61E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:-7.15|P-value:4.26E-13||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.4|P-value:5.51E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAL066W(YAL066W_d)|FD-Score:-4.43|P-value:4.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR139W(YBR139W)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YBT1(YLL048C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCR102C(YCR102C_p)|FD-Score:-3.96|P-value:3.81E-5||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR056C(YDR056C_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YGR053C(YGR053C_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function Gene:YGR130C(YGR130C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YJL067W(YJL067W_d)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL182C(YJL182C_d)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YKL053W(YKL053W_d)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YNL057W(YNL057W_d)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR005C(YNR005C_d)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR053W(YOR053W_d)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPL114W(YPL114W_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR117W(YPR117W_p)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Putative protein of unknown function Gene:YPT6(YLR262C)|FD-Score:-6.66|P-value:1.35E-11||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR072W8.431.72E-172.94ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YLR215C5.491.98E-80.34CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
YHR007C5.161.25E-70.07ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YOR319W5.091.78E-70.86HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YNL114C_d4.241.14E-50.27YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YPR181C3.963.74E-50.22SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YLR196W3.749.28E-50.08PWP1Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
YGR119C3.661.26E-40.00NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YNL161W3.661.27E-40.01CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YPR034W3.651.33E-40.06ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOR157C3.581.69E-40.14PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YJL090C3.452.83E-40.04DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YOR048C3.413.23E-40.12RAT1Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
YBR152W3.294.93E-40.01SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YLR166C3.295.02E-40.09SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML075C6.494.26E-11HMG1HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
YLL048C5.191.03E-7YBT1Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
YCR077C5.121.52E-7PAT1Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress
YNL059C5.091.80E-7ARP5Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YML034W4.943.95E-7SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YIL074C4.681.43E-6SER333-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication
YEL005C4.424.92E-6VAB2Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter
YPR184W4.415.18E-6GDB1Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress
YHR121W4.385.84E-6LSM12Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
YER088C4.385.95E-6DOT6Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YIL073C4.376.30E-6SPO22Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis
YJR118C4.327.87E-6ILM1Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
YOR070C4.082.24E-5GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YMR157C4.062.46E-5AIM36Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies
YOR030W4.032.77E-5DFG16Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p

GO enrichment analysis for SGTC_2185
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2265.05E-69SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.291667ergosterol biosynthesis
0.2251.28E-68SGTC_21555570423 76.0 μMChembridge (Fragment library)7974020.589744ergosterol biosynthesis
0.2217.37E-66SGTC_21475545932 89.7 μMChembridge (Fragment library)7974040.395349ergosterol biosynthesis
0.2057.16E-57SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.164557azole & statin
0.1991.16E-53SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0793651ergosterol biosynthesis
0.1711.06E-39SGTC_23779073351 62.6 μMChembridge (Fragment library)73379460.259259ergosterol biosynthesis
0.1641.34E-36SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride 61.8 μMTimTec (Natural product derivative library)170377580.174603ergosterol biosynthesis
0.1631.62E-36SGTC_5554296-0685 37.0 μMChemDiv (Drug-like library)29085400.128571ergosterol biosynthesis
0.1606.63E-35SGTC_2647atorvastatin 60.2 μMMiscellaneous608230.164557azole & statin
0.1521.06E-31SGTC_29749094440 9.7 μMChembridge (Drug-like library)252361340.149254ergosterol biosynthesis
0.1511.45E-31SGTC_1638st008370 60.6 μMTimTec (Natural product derivative library)242071340.15625ergosterol biosynthesis
0.1491.49E-30SGTC_2753fluvastatin 17.1 μMMiscellaneous4461550.180556azole & statin
0.1483.11E-30SGTC_1547harmine 94.2 μMMiscellaneous52809530.0655738ergosterol biosynthesis
0.1461.33E-29SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.122807ergosterol biosynthesis
0.1433.21E-28SGTC_2525harmine 47.9 μMMiscellaneous52809530.0655738ergosterol biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2155557042376 μM0.589744797402Chembridge (Fragment library)250.3118632.54403ergosterol biosynthesis
SGTC_2376907292281.32 μM0.48936217087968Chembridge (Fragment library)281.78112.2513
SGTC_23849036245200 μM0.4772736462766Chembridge (Fragment library)261.362621.83713
SGTC_1460k062-00599.15 μM0.448981373085ChemDiv (Drug-like library)368.2328434.31513Golgi
SGTC_3219913111249.47 μM0.41666717612988Chembridge (Drug-like library)298.3546633.4513Golgi
SGTC_16794130-12766.7 μM0.4150941005053Miscellaneous363.7707033.63705Golgi
SGTC_2147554593289.72 μM0.395349797404Chembridge (Fragment library)232.32142.33802ergosterol biosynthesis
SGTC_22677931562200 μM0.36934880Chembridge (Fragment library)267.754521.79313
SGTC_23417987486176.74 μM0.361258686Chembridge (Fragment library)267.754521.79313
SGTC_3259913848549.47 μM0.3617646153Chembridge (Drug-like library)334.4083633.15315