mitoxanthrone

1,4-dihydroxy-5,8-bis[2-(2-hydroxyethylamino)ethylamino]anthracene-9,10-dione

Mitoxantrone is a cytostatic anthracenedione that intercalates in DNA and increases the incidence of double-strand breaks by stabilizing the cleavable complex of topoisomerase II and DNA. 

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PubChem MeSH terms: Analgesics;Antineoplastic Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_219
Screen concentration 164.4 μM
Source Miscellaneous
PubChem CID 4212
SMILES C1=CC(=C2C(=C1NCCNCCO)C(=O)C3=C(C=CC(=C3C2=O)O)O)NCCNCCO
Standardized SMILES OCCNCCNc1ccc(NCCNCCO)c2C(=O)c3c(O)ccc(O)c3C(=O)c12
Molecular weight 444.4809
ALogP 0.07
H-bond donor count 8
H-bond acceptor count 10
Response signature DNA intercalators

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.63
% growth inhibition (Hom. pool) 8.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4212
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.14||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CEP3(YMR168C)|FD-Score:21.9|P-value:2.05E-106|Clearance:11.03||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CWC23(YGL128C)|FD-Score:6.37|P-value:9.16E-11|Clearance:1.3||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:FAP7(YDL166C)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.03||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:GCR1(YPL075W)|FD-Score:6.56|P-value:2.66E-11|Clearance:0.12||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:IRA1(YBR140C)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.08||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:NUP145(YGL092W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.07||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:ORC2(YBR060C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.03||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:PAB1(YER165W)|FD-Score:3.1|P-value:9.63E-4|Clearance:0.05||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PDS1(YDR113C)|FD-Score:6.44|P-value:5.97E-11|Clearance:0.07||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:RBA50(YDR527W)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.31||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RPL30(YGL030W)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPS5(YJR123W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.23||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RSC8(YFR037C)|FD-Score:8.33|P-value:4.07E-17|Clearance:1.77||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC1(YDR164C)|FD-Score:10.8|P-value:1.03E-27|Clearance:2.52||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SMD3(YLR147C)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.07||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC97(YHR172W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.06||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:STN1(YDR082W)|FD-Score:4.95|P-value:3.77E-7|Clearance:0.21||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:TFB4(YPR056W)|FD-Score:4.33|P-value:7.53E-6|Clearance:0.11||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TFC1(YBR123C)|FD-Score:5.07|P-value:1.99E-7|Clearance:0.12||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:YKL083W(YKL083W_d)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.33||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:ARP4(YJL081C)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.14||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CEP3(YMR168C)|FD-Score:21.9|P-value:2.05E-106|Clearance:11.03||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CWC23(YGL128C)|FD-Score:6.37|P-value:9.16E-11|Clearance:1.3||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:FAP7(YDL166C)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.03||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:GCR1(YPL075W)|FD-Score:6.56|P-value:2.66E-11|Clearance:0.12||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:IRA1(YBR140C)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.08||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:NUP145(YGL092W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.07||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:ORC2(YBR060C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.03||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:PAB1(YER165W)|FD-Score:3.1|P-value:9.63E-4|Clearance:0.05||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PDS1(YDR113C)|FD-Score:6.44|P-value:5.97E-11|Clearance:0.07||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:RBA50(YDR527W)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.31||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RPL30(YGL030W)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPS5(YJR123W)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.23||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RSC8(YFR037C)|FD-Score:8.33|P-value:4.07E-17|Clearance:1.77||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC1(YDR164C)|FD-Score:10.8|P-value:1.03E-27|Clearance:2.52||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SMD3(YLR147C)|FD-Score:3.17|P-value:7.53E-4|Clearance:0.07||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC97(YHR172W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.06||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:STN1(YDR082W)|FD-Score:4.95|P-value:3.77E-7|Clearance:0.21||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:TFB4(YPR056W)|FD-Score:4.33|P-value:7.53E-6|Clearance:0.11||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TFC1(YBR123C)|FD-Score:5.07|P-value:1.99E-7|Clearance:0.12||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:YKL083W(YKL083W_d)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.33||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4212
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ACM1(YPL267W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADH4(YGL256W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AEP1(YMR064W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ATG15(YCR068W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATP2(YJR121W)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BIO5(YNR056C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CTR1(YPR124W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYT2(YKL087C)|FD-Score:8.39|P-value:2.45E-17||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DIT1(YDR403W)|FD-Score:5.47|P-value:2.30E-8||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DPB3(YBR278W)|FD-Score:4.83|P-value:6.76E-7||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:EFM1(YHL039W)|FD-Score:20.1|P-value:1.67E-90||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EMP24(YGL200C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ENB1(YOL158C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:FUM1(YPL262W)|FD-Score:14.5|P-value:5.14E-48||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GLC3(YEL011W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GTR1(YML121W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HOT13(YKL084W)|FD-Score:6.57|P-value:2.49E-11||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HUL4(YJR036C)|FD-Score:6.61|P-value:1.94E-11||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:ILM1(YJR118C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IMG1(YCR046C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IOC4(YMR044W)|FD-Score:11.2|P-value:1.31E-29||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:JNM1(YMR294W)|FD-Score:-4.49|P-value:3.56E-6||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:MCM22(YJR135C)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MET13(YGL125W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MMS4(YBR098W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MON1(YGL124C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRE11(YMR224C)|FD-Score:4.77|P-value:9.42E-7||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRP20(YDR405W)|FD-Score:10.4|P-value:9.79E-26||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NOP12(YOL041C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OPY1(YBR129C)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PDR15(YDR406W)|FD-Score:10.1|P-value:3.28E-24||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PDR17(YNL264C)|FD-Score:5.83|P-value:2.69E-9||SGD DESC:Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Gene:PSK1(YAL017W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:PTK1(YKL198C)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:PUS5(YLR165C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAD50(YNL250W)|FD-Score:4.8|P-value:7.78E-7||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD51(YER095W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD54(YGL163C)|FD-Score:5.65|P-value:8.14E-9||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:7.69|P-value:7.55E-15||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:11.7|P-value:9.73E-32||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:ROM1(YGR070W)|FD-Score:9.08|P-value:5.49E-20||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL29(YFR032C-A)|FD-Score:9.39|P-value:2.89E-21||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RRT5(YFR032C_p)|FD-Score:8.21|P-value:1.11E-16||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RSM7(YJR113C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC6(YPL183W-A)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTT109(YLL002W)|FD-Score:3.88|P-value:5.20E-5||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SAE2(YGL175C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAP4(YGL229C)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SAS5(YOR213C)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SHE3(YBR130C)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SPC1(YJR010C-A)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:STE24(YJR117W)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SWM2(YNR004W_p)|FD-Score:4.82|P-value:7.17E-7||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TAZ1(YPR140W)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome Gene:TDA11(YHR159W_p)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA4(YJR116W_p)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele Gene:TEF4(YKL081W)|FD-Score:4.52|P-value:3.06E-6||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TSR3(YOR006C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:TUL1(YKL034W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBP5(YER144C)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VMA13(YPR036W)|FD-Score:5.31|P-value:5.49E-8||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS21(YOR089C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:XRS2(YDR369C)|FD-Score:5.3|P-value:5.70E-8||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YAR030C(YAR030C_d)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YBR196C-B(YBR196C-B_p)|FD-Score:4.81|P-value:7.42E-7||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR336W(YDR336W_p)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YGL041C(YGL041C_d)|FD-Score:4.92|P-value:4.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL118C(YGL118C_d)|FD-Score:4.47|P-value:4.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL177W(YGL177W_d)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR067C(YGR067C_p)|FD-Score:7.1|P-value:6.02E-13||SGD DESC:Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p Gene:YGR069W(YGR069W_d)|FD-Score:8.36|P-value:3.04E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR111C(YJR111C_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YJR115W(YJR115W_p)|FD-Score:9.8|P-value:5.91E-23||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YJR154W(YJR154W_p)|FD-Score:5.02|P-value:2.63E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR271W(YLR271W_p)|FD-Score:9.92|P-value:1.68E-23||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR419W(YLR419W_p)|FD-Score:4.89|P-value:5.04E-7||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR166C(YMR166C_p)|FD-Score:17|P-value:6.43E-65||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YPL197C(YPL197C_d)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPR014C(YPR014C_d)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:ZRT3(YKL175W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency Gene:ABZ2(YMR289W)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ACM1(YPL267W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADH4(YGL256W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AEP1(YMR064W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ATG15(YCR068W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATP2(YJR121W)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BIO5(YNR056C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CTR1(YPR124W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYT2(YKL087C)|FD-Score:8.39|P-value:2.45E-17||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DIT1(YDR403W)|FD-Score:5.47|P-value:2.30E-8||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DPB3(YBR278W)|FD-Score:4.83|P-value:6.76E-7||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:EFM1(YHL039W)|FD-Score:20.1|P-value:1.67E-90||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EMP24(YGL200C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ENB1(YOL158C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:FUM1(YPL262W)|FD-Score:14.5|P-value:5.14E-48||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GLC3(YEL011W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GTR1(YML121W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HOT13(YKL084W)|FD-Score:6.57|P-value:2.49E-11||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HUL4(YJR036C)|FD-Score:6.61|P-value:1.94E-11||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:ILM1(YJR118C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IMG1(YCR046C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IOC4(YMR044W)|FD-Score:11.2|P-value:1.31E-29||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:JNM1(YMR294W)|FD-Score:-4.49|P-value:3.56E-6||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:MCM22(YJR135C)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MET13(YGL125W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MMS4(YBR098W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MON1(YGL124C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRE11(YMR224C)|FD-Score:4.77|P-value:9.42E-7||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRP20(YDR405W)|FD-Score:10.4|P-value:9.79E-26||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NOP12(YOL041C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OPY1(YBR129C)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PDR15(YDR406W)|FD-Score:10.1|P-value:3.28E-24||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PDR17(YNL264C)|FD-Score:5.83|P-value:2.69E-9||SGD DESC:Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Gene:PSK1(YAL017W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:PTK1(YKL198C)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:PUS5(YLR165C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAD50(YNL250W)|FD-Score:4.8|P-value:7.78E-7||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RAD51(YER095W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD54(YGL163C)|FD-Score:5.65|P-value:8.14E-9||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:7.69|P-value:7.55E-15||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:11.7|P-value:9.73E-32||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RAD59(YDL059C)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:ROM1(YGR070W)|FD-Score:9.08|P-value:5.49E-20||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL29(YFR032C-A)|FD-Score:9.39|P-value:2.89E-21||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RRT5(YFR032C_p)|FD-Score:8.21|P-value:1.11E-16||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RSM7(YJR113C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC6(YPL183W-A)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTT109(YLL002W)|FD-Score:3.88|P-value:5.20E-5||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SAE2(YGL175C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAP4(YGL229C)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SAS5(YOR213C)|FD-Score:-3.15|P-value:8.09E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SHE3(YBR130C)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SPC1(YJR010C-A)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:STE24(YJR117W)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SWM2(YNR004W_p)|FD-Score:4.82|P-value:7.17E-7||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TAZ1(YPR140W)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome Gene:TDA11(YHR159W_p)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA4(YJR116W_p)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele Gene:TEF4(YKL081W)|FD-Score:4.52|P-value:3.06E-6||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TSR3(YOR006C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:TUL1(YKL034W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:UBP5(YER144C)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VMA13(YPR036W)|FD-Score:5.31|P-value:5.49E-8||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS21(YOR089C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:XRS2(YDR369C)|FD-Score:5.3|P-value:5.70E-8||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YAR030C(YAR030C_d)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YBR196C-B(YBR196C-B_p)|FD-Score:4.81|P-value:7.42E-7||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR336W(YDR336W_p)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YGL041C(YGL041C_d)|FD-Score:4.92|P-value:4.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL118C(YGL118C_d)|FD-Score:4.47|P-value:4.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL177W(YGL177W_d)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR067C(YGR067C_p)|FD-Score:7.1|P-value:6.02E-13||SGD DESC:Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p Gene:YGR069W(YGR069W_d)|FD-Score:8.36|P-value:3.04E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR111C(YJR111C_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YJR115W(YJR115W_p)|FD-Score:9.8|P-value:5.91E-23||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YJR154W(YJR154W_p)|FD-Score:5.02|P-value:2.63E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR271W(YLR271W_p)|FD-Score:9.92|P-value:1.68E-23||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR419W(YLR419W_p)|FD-Score:4.89|P-value:5.04E-7||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR166C(YMR166C_p)|FD-Score:17|P-value:6.43E-65||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YPL197C(YPL197C_d)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPR014C(YPR014C_d)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:ZRT3(YKL175W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR168C21.902.05E-10611.00CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YDR164C10.801.03E-272.52SEC1Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
YFR037C8.334.07E-171.77RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YPL075W6.562.66E-110.12GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YDR113C6.445.97E-110.07PDS1Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YGL128C6.379.16E-111.30CWC23Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p
YBR123C5.071.99E-70.12TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YDR082W4.953.77E-70.21STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YBR140C4.731.10E-60.08IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YKL083W_d4.651.62E-60.33YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YPR056W4.337.53E-60.11TFB4Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
YGL092W4.211.26E-50.07NUP145Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
YGL030W4.141.72E-50.18RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YDR527W3.963.73E-50.31RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YDL166C3.661.28E-40.03FAP7Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHL039W20.101.67E-90EFM1Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YMR166C_p17.006.43E-65YMR166C_pPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
YPL262W14.505.14E-48FUM1Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
YDR004W11.709.73E-32RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YMR044W11.201.31E-29IOC4Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
YDR405W10.409.79E-26MRP20Mitochondrial ribosomal protein of the large subunit
YDR406W10.103.28E-24PDR15Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
YLR271W_p9.921.68E-23YLR271W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YJR115W_p9.805.91E-23YJR115W_pPutative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
YFR032C-A9.392.89E-21RPL29Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
YGR070W9.085.49E-20ROM1GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
YKL087C8.392.45E-17CYT2Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
YGR069W_d8.363.04E-17YGR069W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YFR032C_p8.211.11E-16RRT5_pPutative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation
YDR076W7.697.55E-15RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p

GO enrichment analysis for SGTC_219
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3603.01E-179SGTC_264promethazine 12.5 mMMiscellaneous60140.0714286DNA intercalators
0.2952.07E-118SGTC_1444161-2749 112.4 μMChemDiv (Drug-like library)7975010.169492DNA intercalators
0.2913.05E-115SGTC_1453737-0113 38.4 μMChemDiv (Drug-like library)68493510.126984DNA intercalators
0.2133.55E-61SGTC_257daunorubicin 18.5 μMMiscellaneous303230.111111anthracycline transcription coupled DNA repair
0.2112.09E-60SGTC_1468k284-1086 134.0 μMChemDiv (Drug-like library)159964570.119403DNA intercalators
0.2011.07E-54SGTC_14873970-0795 2.8 μMChemDiv (Drug-like library)37651030.0862069DNA intercalators
0.1911.53E-49SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.0892857DNA intercalators
0.1832.08E-45SGTC_15153460-0031 42.1 μMChemDiv (Drug-like library)28765960.108108DNA intercalators
0.1811.25E-44SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.0263158DNA intercalators
0.1771.57E-42SGTC_1519esterone 74.0 μMTimTec (Pure natural product library)6980.107692DNA intercalators
0.1765.93E-42SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.0724638DNA intercalators
0.1718.05E-40SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.122807DNA intercalators
0.1556.14E-33SGTC_1250133-0007 11.8 μMChemDiv (Drug-like library)265410.0666667DNA intercalators
0.1504.22E-31SGTC_1079idarubicin 2.4 μMNIH Clinical Collection6363620.108434DNA damage response
0.1434.04E-28SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.0945946

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2671danthron100 μM0.3076922950Microsource (Natural product library)240.210922.32424DNA damage response
SGTC_20375169046200 μM0.2549025718022Chembridge (Fragment library)204.22518-0.0923460S ribosome export
SGTC_263alizarin107.93 μM0.256293Miscellaneous240.210922.32424iron homeostasis
SGTC_2686alizarin95.37 μM0.256293Miscellaneous240.210922.32424RPP1 & pyrimidine depletion
SGTC_2507purpurin79.67 μM0.256683Microsource (Natural product library)256.210322.08235copper-dependent oxidative stress
SGTC_1530rhein70.4 μM0.23529410168TimTec (Pure natural product library)284.220421.95436
SGTC_1556plumbagin1.48 μM0.23404310205TimTec (Pure natural product library)188.179421.96213superoxide
SGTC_2575plumbagin510 nM0.23404310205TimTec (Pure natural product library)188.179421.96213superoxide
SGTC_2682pyrogallin40.03 μM0.234043822798Microsource (Natural product library)204.178820.2134copper-dependent oxidative stress
SGTC_2597laccaic acid a100 μM0.2272735491415Microsource (Natural product library)537.428561.824812
SGTC_501nsc-95397161 μM0.222222262093ICCB bioactive library310.388561.17126
SGTC_8890919-152482.7 μM0.23905452ChemDiv (Drug-like library)274.271962.85825