6573537

2-amino-3-(2-methylanilino)naphthalene-1,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2196
Screen concentration 42.7 μM
Source Chembridge (Fragment library)
PubChem CID 2903087
SMILES CC1=CC=CC=C1NC2=C(C(=O)C3=CC=CC=C3C2=O)N
Standardized SMILES Cc1ccccc1N=C2C(=C(O)c3ccccc3C2=O)N
Molecular weight 278.3053
ALogP 2.14
H-bond donor count 2
H-bond acceptor count 4
Response signature copper-dependent oxidative stress

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.46
% growth inhibition (Hom. pool) 6.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2903087
Download HIP data (tab-delimited text)  (excel)
Gene:ECO1(YFR027W)|FD-Score:4.04|P-value:2.67E-5|Clearance:0.44||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:HEM12(YDR047W)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.05||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:KAE1(YKR038C)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.02||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:LCB1(YMR296C)|FD-Score:3.11|P-value:9.43E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:RPS15(YOL040C)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP14(YKL082C)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.44||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RSC58(YLR033W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC13(YLR208W)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.06||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TAP42(YMR028W)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.08||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:YDL221W(YDL221W_d)|FD-Score:3.34|P-value:4.21E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:YIP1(YGR172C)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.04||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:ECO1(YFR027W)|FD-Score:4.04|P-value:2.67E-5|Clearance:0.44||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:HEM12(YDR047W)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.05||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:KAE1(YKR038C)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.02||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:LCB1(YMR296C)|FD-Score:3.11|P-value:9.43E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:RPS15(YOL040C)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP14(YKL082C)|FD-Score:4.15|P-value:1.66E-5|Clearance:0.44||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RSC58(YLR033W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC13(YLR208W)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.06||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TAP42(YMR028W)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.08||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:YDL221W(YDL221W_d)|FD-Score:3.34|P-value:4.21E-4|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:YIP1(YGR172C)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.04||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2903087
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ATG15(YCR068W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BCH1(YMR237W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BST1(YFL025C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:4.96|P-value:3.49E-7||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCS1(YMR038C)|FD-Score:6.52|P-value:3.43E-11||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DPB3(YBR278W)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ECM19(YLR390W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP43(YGR243W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GUT1(YHL032C)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HIR1(YBL008W)|FD-Score:-4.3|P-value:8.35E-6||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HOP2(YGL033W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HUR1(YGL168W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:ILM1(YJR118C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IMP2'(YIL154C)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISY1(YJR050W)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:LDB7(YBL006C)|FD-Score:-4.46|P-value:4.16E-6||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MAC1(YMR021C)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRPL13(YKR006C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:OCH1(YGL038C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:PAU23(YLR037C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PFK1(YGR240C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PRD1(YCL057W)|FD-Score:4.77|P-value:9.22E-7||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SOD1(YJR104C)|FD-Score:6.37|P-value:9.18E-11||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:TED1(YIL039W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRP1(YDR007W)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VMA1(YDL185W)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS25(YJR102C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS8(YAL002W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YCR064C(YCR064C_d)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR455C(YDR455C_d)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR476C(YDR476C_p)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YGR291C(YGR291C_d)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHK8(YHR048W_p)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YLR040C(YLR040C_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YMR124W(YMR124W_p)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YNL115C(YNL115C_p)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YOR052C(YOR052C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPR123C(YPR123C_d)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ACO1(YLR304C)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ATG15(YCR068W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BCH1(YMR237W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BST1(YFL025C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:4.96|P-value:3.49E-7||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCS1(YMR038C)|FD-Score:6.52|P-value:3.43E-11||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DPB3(YBR278W)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:ECM19(YLR390W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP43(YGR243W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GUT1(YHL032C)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HIR1(YBL008W)|FD-Score:-4.3|P-value:8.35E-6||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HOP2(YGL033W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HUR1(YGL168W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:ILM1(YJR118C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IMP2'(YIL154C)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISY1(YJR050W)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:LDB7(YBL006C)|FD-Score:-4.46|P-value:4.16E-6||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MAC1(YMR021C)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRPL13(YKR006C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:OCH1(YGL038C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:PAU23(YLR037C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PFK1(YGR240C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PRD1(YCL057W)|FD-Score:4.77|P-value:9.22E-7||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SOD1(YJR104C)|FD-Score:6.37|P-value:9.18E-11||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:TED1(YIL039W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRP1(YDR007W)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VMA1(YDL185W)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS25(YJR102C)|FD-Score:3.97|P-value:3.65E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS8(YAL002W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YCR064C(YCR064C_d)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR455C(YDR455C_d)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR476C(YDR476C_p)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YGR291C(YGR291C_d)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHK8(YHR048W_p)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YLR040C(YLR040C_p)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YMR124W(YMR124W_p)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YNL115C(YNL115C_p)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene Gene:YOR052C(YOR052C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPR123C(YPR123C_d)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL082C4.151.66E-50.44RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YFR027W4.042.67E-50.44ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YLR033W3.601.59E-40.09RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YGR172C3.512.23E-40.04YIP1Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
YDR047W3.472.57E-40.05HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YKR038C3.423.14E-40.02KAE1Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription
YLR208W3.403.39E-40.06SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YDL221W_d3.344.21E-40.15YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YOL040C3.197.07E-40.08RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YMR296C3.119.43E-41.55E-4LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YMR028W3.119.43E-40.08TAP42Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
YGL247W3.030.001220.04BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YKL141W2.990.001390.09SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
YGR140W2.900.001880.11CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YOL078W2.790.002630.04AVO1Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR038C6.523.43E-11CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YJR104C6.379.18E-11SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YAL021C4.963.49E-7CCR4Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
YCL057W4.779.22E-7PRD1Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress
YLR037C4.612.02E-6PAU23Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
YMR124W_p4.298.98E-6YMR124W_pProtein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
YJR102C3.973.65E-5VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YMR021C3.846.25E-5MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YDL185W3.826.69E-5VMA1Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
YPR123C_d3.739.46E-5YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YJR118C3.721.01E-4ILM1Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
YPL065W3.631.42E-4VPS28Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
YCR068W3.621.46E-4ATG15Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway
YLR390W3.591.62E-4ECM19Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR237W3.522.14E-4BCH1Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2196
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2073.24E-58SGTC_10014048-4355 44.4 μMChemDiv (Drug-like library)218242330.106061copper-dependent oxidative stress
0.2022.23E-55SGTC_10004048-0884 8.7 μMChemDiv (Drug-like library)130400880.0606061copper-dependent oxidative stress
0.1845.26E-46SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.210526copper-dependent oxidative stress
0.1761.56E-42SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.255814copper-dependent oxidative stress
0.1716.52E-40SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.136364copper-dependent oxidative stress
0.1652.14E-37SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.23913superoxide
0.1653.17E-37SGTC_4982,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 150.0 μMICCB bioactive library23740.0714286Golgi
0.1603.76E-35SGTC_20805212524 12.4 μMChembridge (Fragment library)2601830.384615copper-dependent oxidative stress
0.1561.64E-33SGTC_1060092-0003 11.6 μMChemDiv (Drug-like library)10721480.244898copper-dependent oxidative stress
0.1527.06E-32SGTC_6704204-0025 119.0 μMChemDiv (Drug-like library)7190390.296296copper-dependent oxidative stress
0.1499.42E-31SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.192308superoxide
0.1461.80E-29SGTC_2671danthron 100.0 μMMicrosource (Natural product library)29500.219512DNA damage response
0.1411.78E-27SGTC_6260214-0009 33.0 μMChemDiv (Drug-like library)34378740.101695copper-dependent oxidative stress
0.1351.84E-25SGTC_2130NSC 7 2.5 μMChembridge (Fragment library)214480.285714copper-dependent oxidative stress
0.1343.55E-25SGTC_524r073-0010 27.8 μMChemDiv (Drug-like library)27721060.0461538

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2080521252412.4 μM0.384615260183Chembridge (Fragment library)221.639721.36113copper-dependent oxidative stress
SGTC_2681menadione3.18 μM0.3421054055Miscellaneous172.180022.20402superoxide
SGTC_394menadione8.33 μM0.3421054055Miscellaneous172.180022.20402superoxide
SGTC_23037774051200 μM0.3269232953184Chembridge (Fragment library)281.30593.01813
SGTC_12580671-004357.1 μM0.3265314298767ChemDiv (Drug-like library)296.772522.72124
SGTC_2159565861173.75 μM0.313726793844Chembridge (Fragment library)289.35281.9234
SGTC_22957971645200 μM0.3111112978226Chembridge (Fragment library)204.268242.09911
SGTC_3274913829849.47 μM0.30188717015756Chembridge (Drug-like library)293.32322.66624
SGTC_6704204-0025119 μM0.296296719039ChemDiv (Drug-like library)341.745183.07115copper-dependent oxidative stress
SGTC_13591582-0056520 nM0.2909093661189ChemDiv (Drug-like library)304.688563.99416
SGTC_290299-000412.98 μM0.28571416871ChemDiv (Drug-like library)188.179421.44503