6590217

3-ethyl-2-methyl-1H-pyrimido[1,2-a]benzimidazol-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2199
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 703109
SMILES CCC1=C(NC2=NC3=CC=CC=C3N2C1=O)C
Standardized SMILES CCc1c(C)nc2nc3ccccc3n2c1O
Molecular weight 227.2618
ALogP 2.94
H-bond donor count 1
H-bond acceptor count 3
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.84
% growth inhibition (Hom. pool) 5.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 703109
Download HIP data (tab-delimited text)  (excel)
Gene:BRF1(YGR246C)|FD-Score:-3.56|P-value:1.83E-4|Clearance:0||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:BRR6(YGL247W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.23||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:ECM16(YMR128W)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:HCA4(YJL033W)|FD-Score:3.95|P-value:3.98E-5|Clearance:0.29||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:IPP1(YBR011C)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:MAS1(YLR163C)|FD-Score:3.15|P-value:8.26E-4|Clearance:0.03||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NDD1(YOR372C)|FD-Score:3.12|P-value:9.01E-4|Clearance:0.11||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:POL1(YNL102W)|FD-Score:3.31|P-value:4.59E-4|Clearance:0.05||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:RHO3(YIL118W)|FD-Score:-3.43|P-value:3.06E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RSC58(YLR033W)|FD-Score:5.84|P-value:2.53E-9|Clearance:1.43||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SNM1(YDR478W)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.12||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:TOM40(YMR203W)|FD-Score:4.41|P-value:5.11E-6|Clearance:0.47||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSR1(YDL060W)|FD-Score:-3.27|P-value:5.43E-4|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YRA1(YDR381W)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.12||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:BRF1(YGR246C)|FD-Score:-3.56|P-value:1.83E-4|Clearance:0||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:BRR6(YGL247W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.23||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:ECM16(YMR128W)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:HCA4(YJL033W)|FD-Score:3.95|P-value:3.98E-5|Clearance:0.29||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:IPP1(YBR011C)|FD-Score:-3.23|P-value:6.28E-4|Clearance:0||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:MAS1(YLR163C)|FD-Score:3.15|P-value:8.26E-4|Clearance:0.03||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NDD1(YOR372C)|FD-Score:3.12|P-value:9.01E-4|Clearance:0.11||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:POL1(YNL102W)|FD-Score:3.31|P-value:4.59E-4|Clearance:0.05||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:RHO3(YIL118W)|FD-Score:-3.43|P-value:3.06E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RSC58(YLR033W)|FD-Score:5.84|P-value:2.53E-9|Clearance:1.43||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SNM1(YDR478W)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.12||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:TOM40(YMR203W)|FD-Score:4.41|P-value:5.11E-6|Clearance:0.47||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSR1(YDL060W)|FD-Score:-3.27|P-value:5.43E-4|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YRA1(YDR381W)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.12||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 703109
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:BCH1(YMR237W)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BCS1(YDR375C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BRO1(YPL084W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CAN1(YEL063C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:COQ10(YOL008W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:GET3(YDL100C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GPB2(YAL056W)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HIR1(YBL008W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:LDB7(YBL006C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MAD2(YJL030W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MSB4(YOL112W)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:NPP2(YEL016C)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:SSN3(YPL042C)|FD-Score:4.77|P-value:9.05E-7||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:VAN1(YML115C)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:YBL059W(YBL059W_p)|FD-Score:3.43|P-value:2.96E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBL094C(YBL094C_d)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YGR269W(YGR269W_d)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YGR290W(YGR290W_d)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YNL205C(YNL205C_d)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR114W(YOR114W_p)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:AAT2(YLR027C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:BCH1(YMR237W)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BCS1(YDR375C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BRO1(YPL084W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CAN1(YEL063C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:COQ10(YOL008W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:GET3(YDL100C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GPB2(YAL056W)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HIR1(YBL008W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:LDB7(YBL006C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MAD2(YJL030W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MSB4(YOL112W)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:NPP2(YEL016C)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:SSN3(YPL042C)|FD-Score:4.77|P-value:9.05E-7||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:VAN1(YML115C)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:YBL059W(YBL059W_p)|FD-Score:3.43|P-value:2.96E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBL094C(YBL094C_d)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YGR269W(YGR269W_d)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YGR290W(YGR290W_d)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YNL205C(YNL205C_d)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR114W(YOR114W_p)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Putative protein of unknown function; null mutant is viable

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W5.842.53E-91.43RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YMR203W4.415.11E-60.47TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YJL033W3.953.98E-50.29HCA4DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
YGL247W3.661.27E-40.23BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YDR381W3.433.02E-40.12YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YNL102W3.314.59E-40.05POL1Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
YDR478W3.265.52E-40.12SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YLR163C3.158.26E-40.03MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YOR372C3.129.01E-40.11NDD1Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
YLL008W3.010.001290.10DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
YOR204W2.910.001790.09DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YFR051C2.820.002420.04RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YGR277C2.780.002720.14CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YLR378C2.640.004160.03SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YDR141C2.610.004500.02DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL042C4.779.05E-7SSN3Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
YML115C4.691.39E-6VAN1Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
YGR290W_d4.032.83E-5YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YMR237W3.865.78E-5BCH1Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication
YBL094C_d3.739.39E-5YBL094C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W
YLR027C3.591.65E-4AAT2Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
YDL100C3.522.17E-4GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
YOL008W3.442.86E-4COQ10Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
YBL059W_p3.432.96E-4YBL059W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
YOR114W_p3.433.05E-4YOR114W_pPutative protein of unknown function; null mutant is viable
YPL084W3.393.47E-4BRO1Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
YEL063C3.285.26E-4CAN1Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
YGR269W_d3.255.69E-4YGR269W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C
YJL030W3.167.97E-4MAD2Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I
YNL205C_d3.148.53E-4YNL205C_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2199
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1223.77E-21SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0555556RSC & ERG11
0.1218.85E-21SGTC_1771327-0068 8.4 μMChemDiv (Drug-like library)153899000.109375
0.1119.35E-18SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.119403RSC complex & mRNA processing
0.1055.29E-16SGTC_21966573537 42.7 μMChembridge (Fragment library)29030870.157895copper-dependent oxidative stress
0.1011.01E-14SGTC_7093807-1509 243.0 μMChemDiv (Drug-like library)18866770.056338RSC complex & mRNA processing
0.1001.41E-14SGTC_7830848-0141 92.7 μMChemDiv (Drug-like library)67550760.0547945RSC & ERG11
0.1001.42E-14SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0847458heme requiring
0.0992.51E-14SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0714286RSC complex & mRNA processing
0.0979.24E-14SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.122807heat shock/prefoldin
0.0971.05E-13SGTC_1831st055328 12.0 μMTimTec (Natural product derivative library)11030680.09375heme biosynthesis & mitochondrial translocase
0.0952.21E-13SGTC_1722st034304 3.7 μMTimTec (Natural product derivative library)40361480.125mitochondrial processes
0.0953.09E-13SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.102564RSC & ERG11
0.0944.72E-13SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0821918heme biosynthesis & mitochondrial translocase
0.0881.63E-11SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.1RSC complex & mRNA processing
0.0872.77E-11SGTC_14534237-0541 195.0 μMChemDiv (Drug-like library)7398920.153846heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_23359018011154.17 μM0.4791676458208Chembridge (Fragment library)227.261822.87713
SGTC_7223534-0461190 μM0.305085658262ChemDiv (Drug-like library)293.362963.9512PDR1
SGTC_3288911969036.22 μM0.30508517595618Chembridge (Drug-like library)295.378843.00702
SGTC_3017908068149.47 μM0.27868916451914Chembridge (Drug-like library)307.389543.14702
SGTC_229benomyl22.87 μM0.26984128780Miscellaneous290.317722.96524tubulin folding & SWR complex
SGTC_5623772-2733112 μM0.2698412876874ChemDiv (Drug-like library)422.260134.17813ERG2
SGTC_8291635-0120117 μM0.26285054ChemDiv (Drug-like library)220.229422.38903DNA damage response
SGTC_7243910-0327137 μM0.2580652885483ChemDiv (Drug-like library)316.782083.59813ERG2
SGTC_3304912567846.09 μM0.25806541316246Chembridge (Drug-like library)281.352262.74602
SGTC_10462001-0062216 μM0.257576739778ChemDiv (Drug-like library)341.427363.60714