paclitaxel FDA approved compound

Paclitaxel is a potent anti-neoplastic and anti-mitotic taxane drug, which binds to the N-terminus of β-tubulin and and stabilizes microtubules arresting the cell cycle at the G2/M phase

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PubChem MeSH terms: Antineoplastic Agents, Phytogenic;Tubulin Modulators



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_220
Screen concentration 373.4 μM
Source Miscellaneous
PubChem CID 4666
SMILES CC1=C2C(C(=O)C3(C(CC4C(C3C(C(C2(C)C)(CC1OC(=O)C(C(C5=CC=CC=C5)NC(=O)C6=CC=CC=C6)O)O)OC(=O)C7=CC=CC=C7)(CO4)OC(=O)C)O)C)OC(=O)C
Standardized SMILES CC(=O)OC1C(=O)C2(C)C(O)CC3OCC3(OC(=O)C)C2C(OC(=O)c4ccccc4)C5(O)CC(OC(=O)C(O)C(NC(=O)c6ccccc6)c7ccccc7)C(=C1C5(C)C)C
Molecular weight 853.9061
ALogP 3.06
H-bond donor count 4
H-bond acceptor count 14
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.72
% growth inhibition (Hom. pool) 1.11


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4666
Download HIP data (tab-delimited text)  (excel)
Gene:ALG13(YGL047W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.05||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:CDS1(YBR029C)|FD-Score:3.24|P-value:5.95E-4|Clearance:0.12||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC2(YDL209C)|FD-Score:-3.73|P-value:9.57E-5|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GLC7(YER133W)|FD-Score:-4.05|P-value:2.60E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:PGI1(YBR196C)|FD-Score:4.41|P-value:5.18E-6|Clearance:0.7||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PRP19(YLL036C)|FD-Score:-3.47|P-value:2.57E-4|Clearance:0||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:YBR124W(YBR124W_d)|FD-Score:5.46|P-value:2.37E-8|Clearance:1.05||SGD DESC:Putative protein of unknown function Gene:YPR136C(YPR136C_d)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.46||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ALG13(YGL047W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.05||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:CDS1(YBR029C)|FD-Score:3.24|P-value:5.95E-4|Clearance:0.12||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC2(YDL209C)|FD-Score:-3.73|P-value:9.57E-5|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GLC7(YER133W)|FD-Score:-4.05|P-value:2.60E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:PGI1(YBR196C)|FD-Score:4.41|P-value:5.18E-6|Clearance:0.7||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PRP19(YLL036C)|FD-Score:-3.47|P-value:2.57E-4|Clearance:0||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:YBR124W(YBR124W_d)|FD-Score:5.46|P-value:2.37E-8|Clearance:1.05||SGD DESC:Putative protein of unknown function Gene:YPR136C(YPR136C_d)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.46||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4666
Download HOP data (tab-delimited text)  (excel)
Gene:ASF1(YJL115W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:CAJ1(YER048C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CBS2(YDR197W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:COQ3(YOL096C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:COQ5(YML110C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CWP2(YKL096W-A)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DLT1(YMR126C_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:GEP4(YHR100C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:INP54(YOL065C)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:MMM1(YLL006W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL27(YBR282W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL51(YPR100W)|FD-Score:4.32|P-value:7.85E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSS2(YDL107W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:NAS2(YIL007C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:PET191(YJR034W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PHO3(YBR092C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:PIB1(YDR313C)|FD-Score:3.8|P-value:7.10E-5||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PPT2(YPL148C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RRT1(YBL048W_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RUP1(YOR138C)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SGF29(YCL010C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SIS2(YKR072C)|FD-Score:-4.31|P-value:8.12E-6||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SPS100(YHR139C)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SSH1(YBR283C)|FD-Score:-3.85|P-value:5.79E-5||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SYO1(YDL063C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TPN1(YGL186C)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TUS1(YLR425W)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBR1(YGR184C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:YDR182W-A(YDR182W-A_p)|FD-Score:4.32|P-value:7.97E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFT2(YDR319C_p)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YIL152W(YIL152W_p)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Putative protein of unknown function Gene:YIR035C(YIR035C_p)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL132W(YJL132W_p)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene Gene:YKL222C(YKL222C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR053C(YLR053C_p)|FD-Score:4.38|P-value:6.03E-6||SGD DESC:Putative protein of unknown function Gene:YLR271W(YLR271W_p)|FD-Score:13.2|P-value:2.97E-40||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR444C(YLR444C_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ASF1(YJL115W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:CAJ1(YER048C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CBS2(YDR197W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:COQ3(YOL096C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:COQ5(YML110C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CWP2(YKL096W-A)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DLT1(YMR126C_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:GEP4(YHR100C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:INP54(YOL065C)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:MMM1(YLL006W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL27(YBR282W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL51(YPR100W)|FD-Score:4.32|P-value:7.85E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSS2(YDL107W)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:NAS2(YIL007C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:PET191(YJR034W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PHO3(YBR092C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:PIB1(YDR313C)|FD-Score:3.8|P-value:7.10E-5||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PPT2(YPL148C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RRT1(YBL048W_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RUP1(YOR138C)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SGF29(YCL010C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SIS2(YKR072C)|FD-Score:-4.31|P-value:8.12E-6||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SPS100(YHR139C)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SSH1(YBR283C)|FD-Score:-3.85|P-value:5.79E-5||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SYO1(YDL063C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:TPN1(YGL186C)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TUS1(YLR425W)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBR1(YGR184C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:YDR182W-A(YDR182W-A_p)|FD-Score:4.32|P-value:7.97E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFT2(YDR319C_p)|FD-Score:3.81|P-value:6.94E-5||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YIL152W(YIL152W_p)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Putative protein of unknown function Gene:YIR035C(YIR035C_p)|FD-Score:-3.33|P-value:4.36E-4||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YJL132W(YJL132W_p)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene Gene:YKL222C(YKL222C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR053C(YLR053C_p)|FD-Score:4.38|P-value:6.03E-6||SGD DESC:Putative protein of unknown function Gene:YLR271W(YLR271W_p)|FD-Score:13.2|P-value:2.97E-40||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR444C(YLR444C_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR124W_d5.462.37E-81.05YBR124W_dPutative protein of unknown function
YBR196C4.415.18E-60.70PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YPR136C_d3.711.05E-40.47YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YBR029C3.245.95E-40.12CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YGL047W3.138.86E-40.05ALG13Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
YLR147C3.070.001060.27SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YOL040C2.810.002510.04RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YBR190W_d2.770.002800.02YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YLR075W2.750.002960.05RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YIL118W2.700.003430.10RHO3Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
YLR167W2.600.004630.05RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YDL196W_d2.560.005280.04YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YGL169W2.510.005950.01SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YLR198C_d2.510.006050.01YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YIL021W2.500.006250.05RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR271W_p13.202.97E-40YLR271W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YKL096W-A4.661.60E-6CWP2Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored
YLR053C_p4.386.03E-6YLR053C_pPutative protein of unknown function
YPR100W4.327.85E-6MRPL51Mitochondrial ribosomal protein of the large subunit
YDR182W-A_p4.327.97E-6YDR182W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YPL148C4.241.13E-5PPT2Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YDR197W4.072.37E-5CBS2Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
YML110C3.973.62E-5COQ52-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
YLR425W3.836.49E-5TUS1Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
YDL107W3.826.76E-5MSS2Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
YDR319C_p3.816.94E-5YFT2_pProtein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
YDR313C3.807.10E-5PIB1RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
YOL096C3.721.01E-4COQ3O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
YBL048W_d3.691.13E-4RRT1_dIdentified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YOR188W3.681.18E-4MSB1Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress

GO enrichment analysis for SGTC_220
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2899.38E-114SGTC_223busulfan 5.0 mMMiscellaneous24780.030303
0.2665.96E-96SGTC_225nsc-122657 1.0 μMMiscellaneous2756850.0614035
0.2471.49E-82SGTC_224benfluorex 43.9 μMMiscellaneous23180.146552
0.2062.22E-57SGTC_222nsc-310342 10.7 μMMiscellaneous4325560.0423729
0.1172.23E-19SGTC_7734358-1457 44.6 μMChemDiv (Drug-like library)11868860.0819672
0.0992.10E-14SGTC_218alverine citrate 93.8 μMMiscellaneous217180.0679612fatty acid desaturase (OLE1)
0.0904.30E-12SGTC_22226885208 172.1 μMChembridge (Fragment library)6095140.12381
0.0774.23E-9SGTC_29035923464 63.9 μMChembridge (Drug-like library)28765760.0932203
0.0764.67E-9SGTC_23839036125 200.0 μMChembridge (Fragment library)795800.0701754
0.0731.70E-8SGTC_9891501-0895 10.3 μMChemDiv (Drug-like library)28301120.0666667
0.0731.95E-8SGTC_1609st001574 46.4 μMTimTec (Natural product derivative library)39600120.144
0.0731.99E-8SGTC_2361509-0011 5.3 μMChemDiv (Drug-like library)31072800.0431034
0.0715.49E-8SGTC_7783456-2434 196.0 μMChemDiv (Drug-like library)55390630.0990991mitochondrial response to ROS
0.0681.91E-7SGTC_219mitoxanthrone 164.4 μMMiscellaneous42120.0630631DNA intercalators
0.0672.33E-7SGTC_9041141-0088 396.0 μMChemDiv (Drug-like library)13781190.117647

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2407paclitaxel400 μM14666Miscellaneous853.906143.055414
SGTC_2419paclitaxel166.5 μM14666Miscellaneous853.906143.055414
SGTC_417st075190100 μM0.186441222757TimTec (Pure natural product library)376.488025.51213
SGTC_2649anisomycin9.56 μM0.1809526610309ICCB bioactive library235.278980.89324
SGTC_395anisomycin6.25 μM0.1809526610309ICCB bioactive library235.278980.89324
SGTC_1079idarubicin2.36 μM0.174603636362NIH Clinical Collection533.954821.026610DNA damage response
SGTC_1756st04155835.5 μM0.1654143955792TimTec (Natural product derivative library)564.649284.77928
SGTC_8810833-0569170 μM0.1651385418700ChemDiv (Drug-like library)306.315362.68414
SGTC_3260913710949.47 μM0.16504917478346Chembridge (Drug-like library)255.311683.1561260S ribosome export
SGTC_2653dihydrofissinolide100 μM0.1641796857746Microsource (Natural product library)514.607223.40808
SGTC_1931st07443857.1 μM0.1637932787313TimTec (Natural product derivative library)350.410983.91323
SGTC_7420kpi-0133140 μM0.1603772728852ChemDiv (Drug-like library)297.434483.98912ERG2