6600604

2-(2,6-dimethylphenyl)-3a,4,7,7a-tetrahydro-octahydro-1H-4,7-epoxyisoindole-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2200
Screen concentration 21.9 μM
Source Chembridge (Fragment library)
PubChem CID 2904777
SMILES CC1=C(C(=CC=C1)C)N2C(=O)C3C4C=CC(C3C2=O)O4
Standardized SMILES Cc1cccc(C)c1N2C(=O)C3C4OC(C=C4)C3C2=O
Molecular weight 269.2952
ALogP 1.82
H-bond donor count 0
H-bond acceptor count 3
Response signature tubulin folding & SWR complex

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.05
% growth inhibition (Hom. pool) 14.2


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2904777
Download HIP data (tab-delimited text)  (excel)
Gene:CCA1(YER168C)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.01||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC34(YDR054C)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.14||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:EFB1(YAL003W)|FD-Score:4.28|P-value:9.28E-6|Clearance:0.16||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:GPI10(YGL142C)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.09||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:HEM4(YOR278W)|FD-Score:4.71|P-value:1.26E-6|Clearance:0.43||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HSH49(YOR319W)|FD-Score:5.57|P-value:1.28E-8|Clearance:0.75||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IFH1(YLR223C)|FD-Score:4.82|P-value:7.30E-7|Clearance:0.11||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:LCB1(YMR296C)|FD-Score:4.12|P-value:1.91E-5|Clearance:0.08||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NIP1(YMR309C)|FD-Score:3.71|P-value:1.06E-4|Clearance:0.34||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:RPB8(YOR224C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:SGV1(YPR161C)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.01||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SPN1(YPR133C)|FD-Score:-3.22|P-value:6.46E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SUI3(YPL237W)|FD-Score:4.03|P-value:2.74E-5|Clearance:0.33||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TUB1(YML085C)|FD-Score:-4.89|P-value:4.96E-7|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDL221W(YDL221W_d)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:CCA1(YER168C)|FD-Score:3.14|P-value:8.52E-4|Clearance:0.01||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC34(YDR054C)|FD-Score:3.36|P-value:3.96E-4|Clearance:0.14||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:EFB1(YAL003W)|FD-Score:4.28|P-value:9.28E-6|Clearance:0.16||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:GPI10(YGL142C)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.09||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:HEM4(YOR278W)|FD-Score:4.71|P-value:1.26E-6|Clearance:0.43||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HSH49(YOR319W)|FD-Score:5.57|P-value:1.28E-8|Clearance:0.75||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IFH1(YLR223C)|FD-Score:4.82|P-value:7.30E-7|Clearance:0.11||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:LCB1(YMR296C)|FD-Score:4.12|P-value:1.91E-5|Clearance:0.08||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:NIP1(YMR309C)|FD-Score:3.71|P-value:1.06E-4|Clearance:0.34||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:RPB8(YOR224C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:SGV1(YPR161C)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.01||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SPN1(YPR133C)|FD-Score:-3.22|P-value:6.46E-4|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SUI3(YPL237W)|FD-Score:4.03|P-value:2.74E-5|Clearance:0.33||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TUB1(YML085C)|FD-Score:-4.89|P-value:4.96E-7|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDL221W(YDL221W_d)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2904777
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ALF1(YNL148C)|FD-Score:5.82|P-value:3.02E-9||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APL5(YPL195W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APL6(YGR261C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARC18(YLR370C)|FD-Score:9.77|P-value:7.76E-23||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARF1(YDL192W)|FD-Score:8.08|P-value:3.30E-16||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARP6(YLR085C)|FD-Score:9.66|P-value:2.13E-22||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BIK1(YCL029C)|FD-Score:6.06|P-value:6.88E-10||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BNA3(YJL060W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BST1(YFL025C)|FD-Score:6.93|P-value:2.08E-12||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHS7(YHR142W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CIN1(YOR349W)|FD-Score:11.9|P-value:3.97E-33||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:6.16|P-value:3.59E-10||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CKA2(YOR061W)|FD-Score:4.42|P-value:5.05E-6||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLA4(YNL298W)|FD-Score:6.35|P-value:1.08E-10||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLC1(YGR167W)|FD-Score:6.77|P-value:6.54E-12||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CYS3(YAL012W)|FD-Score:5.77|P-value:3.98E-9||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DGR2(YKL121W_p)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DOA4(YDR069C)|FD-Score:6.56|P-value:2.74E-11||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DPH1(YIL103W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:EAF7(YNL136W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ECM18(YDR125C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ECM25(YJL201W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:END3(YNL084C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:EOS1(YNL080C)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERG2(YMR202W)|FD-Score:7.64|P-value:1.06E-14||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:15.3|P-value:6.29E-53||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:GCV3(YAL044C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GIP1(YBR045C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GLE2(YER107C)|FD-Score:4.43|P-value:4.81E-6||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GRE3(YHR104W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GSH1(YJL101C)|FD-Score:5.86|P-value:2.33E-9||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HPF1(YOL155C)|FD-Score:4.33|P-value:7.32E-6||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HTZ1(YOL012C)|FD-Score:7.33|P-value:1.19E-13||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IRS4(YKR019C)|FD-Score:-4.88|P-value:5.43E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KRE6(YPR159W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LAG1(YHL003C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LEM3(YNL323W)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LTE1(YAL024C)|FD-Score:6.04|P-value:7.47E-10||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MIG3(YER028C)|FD-Score:-5.05|P-value:2.19E-7||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MMM1(YLL006W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MXR2(YCL033C)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NUC1(YJL208C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:OPI9(YLR338W_d)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAC2(YER007W)|FD-Score:16.7|P-value:1.07E-62||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PDH1(YPR002W)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PEP12(YOR036W)|FD-Score:6.19|P-value:2.98E-10||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET20(YPL159C)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PRE9(YGR135W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:RAD1(YPL022W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD51(YER095W)|FD-Score:4.32|P-value:7.68E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RBK1(YCR036W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Putative ribokinase Gene:REF2(YDR195W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RGP1(YDR137W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM1(YPL208W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RPN4(YDL020C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RVS161(YCR009C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC6(YDR129C)|FD-Score:16.2|P-value:5.54E-59||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SFT2(YBL102W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SHE4(YOR035C)|FD-Score:13|P-value:5.79E-39||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIF2(YBR103W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SLT2(YHR030C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF7(YLR025W)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:STP22(YCL008C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUL2(YLR092W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUR4(YLR372W)|FD-Score:6.49|P-value:4.43E-11||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC3(YAL011W)|FD-Score:8.08|P-value:3.19E-16||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWC5(YBR231C)|FD-Score:8.89|P-value:3.16E-19||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:13.3|P-value:2.21E-40||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TMA7(YLR262C-A)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:18.4|P-value:3.09E-76||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:14.5|P-value:3.83E-48||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:URA7(YBL039C)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:5.37|P-value:3.90E-8||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAM6(YDL077C)|FD-Score:4.6|P-value:2.11E-6||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VPS25(YJR102C)|FD-Score:5.65|P-value:7.92E-9||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS3(YDR495C)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS30(YPL120W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS36(YLR417W)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:7.34|P-value:1.05E-13||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS71(YML041C)|FD-Score:20|P-value:1.01E-89||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:11.7|P-value:9.14E-32||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS8(YAL002W)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YAF9(YNL107W)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YAP1(YML007W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL050C(YDL050C_d)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:-3.8|P-value:7.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR455C(YDR455C_d)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGR064W(YGR064W_d)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YLR280C(YLR280C_d)|FD-Score:-3.85|P-value:5.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML037C(YML037C_p)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:6.38|P-value:8.88E-11||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPT53(YNL093W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ACO1(YLR304C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ALF1(YNL148C)|FD-Score:5.82|P-value:3.02E-9||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APL5(YPL195W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APL6(YGR261C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARC18(YLR370C)|FD-Score:9.77|P-value:7.76E-23||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARF1(YDL192W)|FD-Score:8.08|P-value:3.30E-16||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARP6(YLR085C)|FD-Score:9.66|P-value:2.13E-22||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BIK1(YCL029C)|FD-Score:6.06|P-value:6.88E-10||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BNA3(YJL060W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BST1(YFL025C)|FD-Score:6.93|P-value:2.08E-12||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHS7(YHR142W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CIN1(YOR349W)|FD-Score:11.9|P-value:3.97E-33||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:6.16|P-value:3.59E-10||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CKA2(YOR061W)|FD-Score:4.42|P-value:5.05E-6||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLA4(YNL298W)|FD-Score:6.35|P-value:1.08E-10||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLC1(YGR167W)|FD-Score:6.77|P-value:6.54E-12||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CYS3(YAL012W)|FD-Score:5.77|P-value:3.98E-9||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DGR2(YKL121W_p)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DOA4(YDR069C)|FD-Score:6.56|P-value:2.74E-11||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DPH1(YIL103W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:EAF7(YNL136W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ECM18(YDR125C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ECM25(YJL201W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:END3(YNL084C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:EOS1(YNL080C)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERG2(YMR202W)|FD-Score:7.64|P-value:1.06E-14||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:15.3|P-value:6.29E-53||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:4.27|P-value:9.97E-6||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:GCV3(YAL044C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GIP1(YBR045C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GLE2(YER107C)|FD-Score:4.43|P-value:4.81E-6||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GRE3(YHR104W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GSH1(YJL101C)|FD-Score:5.86|P-value:2.33E-9||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HPF1(YOL155C)|FD-Score:4.33|P-value:7.32E-6||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HTZ1(YOL012C)|FD-Score:7.33|P-value:1.19E-13||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IRS4(YKR019C)|FD-Score:-4.88|P-value:5.43E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KRE6(YPR159W)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LAG1(YHL003C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LEM3(YNL323W)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LTE1(YAL024C)|FD-Score:6.04|P-value:7.47E-10||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MIG3(YER028C)|FD-Score:-5.05|P-value:2.19E-7||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MMM1(YLL006W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MXR2(YCL033C)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NUC1(YJL208C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:OPI9(YLR338W_d)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAC2(YER007W)|FD-Score:16.7|P-value:1.07E-62||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PDH1(YPR002W)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PEP12(YOR036W)|FD-Score:6.19|P-value:2.98E-10||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET20(YPL159C)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PRE9(YGR135W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:RAD1(YPL022W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD51(YER095W)|FD-Score:4.32|P-value:7.68E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RBK1(YCR036W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Putative ribokinase Gene:REF2(YDR195W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RGP1(YDR137W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM1(YPL208W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RPN4(YDL020C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RVS161(YCR009C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAC6(YDR129C)|FD-Score:16.2|P-value:5.54E-59||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SFT2(YBL102W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SHE4(YOR035C)|FD-Score:13|P-value:5.79E-39||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIF2(YBR103W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SLT2(YHR030C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF7(YLR025W)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:STP22(YCL008C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUL2(YLR092W)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUR4(YLR372W)|FD-Score:6.49|P-value:4.43E-11||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC3(YAL011W)|FD-Score:8.08|P-value:3.19E-16||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWC5(YBR231C)|FD-Score:8.89|P-value:3.16E-19||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:13.3|P-value:2.21E-40||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TMA7(YLR262C-A)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:18.4|P-value:3.09E-76||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:14.5|P-value:3.83E-48||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:URA7(YBL039C)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:5.37|P-value:3.90E-8||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAM6(YDL077C)|FD-Score:4.6|P-value:2.11E-6||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VPS25(YJR102C)|FD-Score:5.65|P-value:7.92E-9||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS3(YDR495C)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS30(YPL120W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS36(YLR417W)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:7.34|P-value:1.05E-13||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS71(YML041C)|FD-Score:20|P-value:1.01E-89||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:11.7|P-value:9.14E-32||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS8(YAL002W)|FD-Score:3.15|P-value:8.28E-4||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YAF9(YNL107W)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YAP1(YML007W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL050C(YDL050C_d)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:-3.8|P-value:7.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR455C(YDR455C_d)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGR064W(YGR064W_d)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YLR280C(YLR280C_d)|FD-Score:-3.85|P-value:5.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML037C(YML037C_p)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:6.38|P-value:8.88E-11||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPT53(YNL093W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR319W5.571.28E-80.75HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YLR223C4.827.30E-70.11IFH1Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
YOR278W4.711.26E-60.42HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YAL003W4.289.28E-60.16EFB1Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
YMR296C4.121.91E-50.08LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YPL237W4.032.74E-50.33SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YMR309C3.711.06E-40.34NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YPR161C3.373.81E-40.01SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YDR054C3.363.96E-40.14CDC34Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
YDL221W_d3.226.49E-40.08YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YER168C3.148.52E-40.01CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YGL142C3.138.86E-40.09GPI10Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
YDL103C3.040.001200.05QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YOL078W2.980.001430.04AVO1Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth
YHR062C2.940.001620.08RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML041C20.001.01E-89VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YBR058C-A18.403.09E-76TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YER007W16.701.07E-62PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YDR129C16.205.54E-59SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YGL012W15.306.29E-53ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YML124C14.503.83E-48TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YDR334W13.302.21E-40SWR1Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YOR035C13.005.79E-39SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YOR349W11.903.97E-33CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YDR485C11.709.14E-32VPS72Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YLR370C9.777.76E-23ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YLR085C9.662.13E-22ARP6Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YBR231C8.893.16E-19SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YAL011W8.083.19E-16SWC3Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
YDL192W8.083.30E-16ARF1ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2200
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6270SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.301887tubulin folding & SWR complex
0.5150SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.164179
0.4990SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.109091
0.4720SGTC_23196033012 152.6 μMChembridge (Fragment library)8472460.0877193tubulin folding & SWR complex
0.4680SGTC_229benomyl 22.9 μMMiscellaneous287800.0895522tubulin folding & SWR complex
0.4528.39E-296SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.0925926
0.4481.67E-289SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.230769
0.4433.84E-282SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.12963TSC3-RPN4
0.4352.71E-271SGTC_21745790901 200.0 μMChembridge (Fragment library)7860200.142857tubulin folding & SWR complex
0.4341.48E-270SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.151515
0.4248.92E-256SGTC_14334060-0023 7.6 μMChemDiv (Drug-like library)38406270.114754tubulin folding & SWR complex
0.4205.24E-251SGTC_2123807-4682 38.8 μMChemDiv (Drug-like library)31255010.15873tubulin folding & SWR complex
0.3995.25E-225SGTC_286k060-0067 22.9 μMChemDiv (Drug-like library)59850510.0724638tubulin folding & SWR complex
0.3961.96E-221SGTC_13261431-2094 10.8 μMChemDiv (Drug-like library)6162780.137255tubulin folding & SWR complex
0.3774.58E-199SGTC_32949122953 26.3 μMChembridge (Drug-like library)164598260.164179tubulin folding & SWR complex

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21735763948167 μM0.6944442867177Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_215856571233.62 μM0.5789472858827Chembridge (Fragment library)269.29521.81603
SGTC_2103537813810 μM0.571429244142Chembridge (Fragment library)241.242040.84403
SGTC_14873970-07952.82 μM0.5253765103ChemDiv (Drug-like library)320.13811.59203DNA intercalators
SGTC_216156625048.51 μM0.5128212859334Chembridge (Fragment library)275.68711.50803tubulin folding & SWR complex
SGTC_14863970-07934.53 μM0.52844330ChemDiv (Drug-like library)320.13811.59203
SGTC_1903970-07903.72 μM0.4523812832352ChemDiv (Drug-like library)286.23960.73805
SGTC_9963970-07991.8 μM0.4444446615648ChemDiv (Drug-like library)299.321181.704
SGTC_20985376439882.5 nM0.3265312844116Chembridge (Fragment library)255.268621.04903
SGTC_23569061756188.97 μM0.30769220104043Chembridge (Fragment library)246.304921.46312