7233102

2-oxo-N-prop-2-enylchromene-3-carboxamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2204
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 2172480
SMILES C=CCNC(=O)C1=CC2=CC=CC=C2OC1=O
Standardized SMILES C=CCNC(=O)C1=Cc2ccccc2OC1=O
Molecular weight 229.2313
ALogP 1.68
H-bond donor count 1
H-bond acceptor count 3
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.39
% growth inhibition (Hom. pool) 6.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2172480
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:3.86|P-value:5.78E-5|Clearance:0.38||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CLP1(YOR250C)|FD-Score:3.25|P-value:5.67E-4|Clearance:0.09||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DPS1(YLL018C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.01||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:EMG1(YLR186W)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:LSG1(YGL099W)|FD-Score:7.72|P-value:5.98E-15|Clearance:1.99||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NMD3(YHR170W)|FD-Score:5.73|P-value:5.12E-9|Clearance:1.38||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PAM16(YJL104W)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.05||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PGS1(YCL004W)|FD-Score:-3.2|P-value:6.83E-4|Clearance:0||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP5(YBR237W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.14||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:SEC9(YGR009C)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SSC1(YJR045C)|FD-Score:-4.18|P-value:1.45E-5|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TAP42(YMR028W)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.02||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:UTP14(YML093W)|FD-Score:-3.21|P-value:6.60E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL221W(YDL221W_d)|FD-Score:4.35|P-value:6.92E-6|Clearance:0.49||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:YLR458W(YLR458W_d)|FD-Score:3.14|P-value:8.51E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:ABF1(YKL112W)|FD-Score:3.86|P-value:5.78E-5|Clearance:0.38||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CLP1(YOR250C)|FD-Score:3.25|P-value:5.67E-4|Clearance:0.09||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DPS1(YLL018C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.01||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:EMG1(YLR186W)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:LSG1(YGL099W)|FD-Score:7.72|P-value:5.98E-15|Clearance:1.99||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NMD3(YHR170W)|FD-Score:5.73|P-value:5.12E-9|Clearance:1.38||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PAM16(YJL104W)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.05||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PGS1(YCL004W)|FD-Score:-3.2|P-value:6.83E-4|Clearance:0||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP5(YBR237W)|FD-Score:3.47|P-value:2.56E-4|Clearance:0.14||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:SEC9(YGR009C)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SSC1(YJR045C)|FD-Score:-4.18|P-value:1.45E-5|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TAP42(YMR028W)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.02||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:UTP14(YML093W)|FD-Score:-3.21|P-value:6.60E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL221W(YDL221W_d)|FD-Score:4.35|P-value:6.92E-6|Clearance:0.49||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Gene:YLR458W(YLR458W_d)|FD-Score:3.14|P-value:8.51E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2172480
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:3.77|P-value:8.33E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ATP17(YDR377W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATS1(YAL020C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:FIS1(YIL065C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FKS3(YMR306W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUM1(YPL262W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:FUS2(YMR232W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GIS4(YML006C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:HMO1(YDR174W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:LDB7(YBL006C)|FD-Score:-6.83|P-value:4.35E-12||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LPD1(YFL018C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MGM101(YJR144W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MRPL10(YNL284C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MTC3(YGL226W)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NEW1(YPL226W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:PGM3(YMR278W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:RAD51(YER095W)|FD-Score:5.27|P-value:6.79E-8||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RPS21B(YJL136C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RPS7B(YNL096C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SDP1(YIL113W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:TCB3(YML072C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:VPS5(YOR069W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YAL004W(YAL004W_d)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YDR015C(YDR015C_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR018C(YDR018C_p)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YEL043W(YEL043W)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YGR069W(YGR069W_d)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR112C(YHR112C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YIL096C(YIL096C_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YKL075C(YKL075C_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:ADE1(YAR015W)|FD-Score:3.77|P-value:8.33E-5||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:ATP17(YDR377W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATS1(YAL020C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:FIS1(YIL065C)|FD-Score:3.32|P-value:4.50E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FKS3(YMR306W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUM1(YPL262W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:FUS2(YMR232W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GIS4(YML006C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:HMO1(YDR174W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:LDB7(YBL006C)|FD-Score:-6.83|P-value:4.35E-12||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LPD1(YFL018C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MGM101(YJR144W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MRPL10(YNL284C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MTC3(YGL226W)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NEW1(YPL226W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:PGM3(YMR278W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:RAD51(YER095W)|FD-Score:5.27|P-value:6.79E-8||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RPS21B(YJL136C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RPS7B(YNL096C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SDP1(YIL113W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:TCB3(YML072C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:VPS5(YOR069W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:YAL004W(YAL004W_d)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YDR015C(YDR015C_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR018C(YDR018C_p)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YEL043W(YEL043W)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YGR069W(YGR069W_d)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR112C(YHR112C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YIL096C(YIL096C_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YKL075C(YKL075C_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W7.725.98E-151.99LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W5.735.12E-91.38NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDL221W_d4.356.92E-60.49YDL221W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
YKL112W3.865.78E-50.38ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YBR237W3.472.56E-40.14PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YER112W3.334.33E-40.01LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YJL104W3.324.52E-40.05PAM16Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
YLL018C3.275.42E-40.01DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YOR250C3.255.67E-40.09CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YMR028W3.167.86E-40.02TAP42Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
YLR458W_d3.148.51E-40.07YLR458W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis
YER126C3.070.001060.29NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YOL021C2.790.002670.03DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YJL011C2.750.002960.07RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YNL306W2.680.003680.04MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER095W5.276.79E-8RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YMR232W4.102.05E-5FUS2Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
YDR377W3.865.75E-5ATP17Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YAR015W3.778.33E-5ADE1N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
YFL018C3.661.28E-4LPD1Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes
YMR278W3.581.73E-4PGM3Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
YJL136C3.551.91E-4RPS21BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
YML006C3.532.09E-4GIS4CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway
YPL226W3.433.05E-4NEW1ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
YDR015C_d3.413.24E-4YDR015C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A
YHR112C3.393.54E-4YHR112CProtein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
YJR144W3.383.67E-4MGM101Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage
YML072C3.363.90E-4TCB3Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
YPL262W3.363.94E-4FUM1Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
YAL004W_d3.344.25E-4YAL004W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C

GO enrichment analysis for SGTC_2204
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2594.53E-91SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.15853760S ribosome export
0.2163.31E-63SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.086956560S ribosome export
0.2146.18E-62SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.098591660S ribosome export
0.2103.87E-60SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.14492860S ribosome export
0.1904.40E-49SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.1298760S ribosome export
0.1783.97E-43SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.32727360S ribosome export
0.1771.19E-42SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.077922160S ribosome export
0.1761.94E-42SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.17741960S ribosome export
0.1716.80E-40SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.0937560S ribosome export
0.1645.30E-37SGTC_1350933-0004 26.3 μMChemDiv (Drug-like library)212306170.11764760S ribosome export
0.1554.46E-33SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.49056660S ribosome export
0.1547.65E-33SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.064102660S ribosome export
0.1491.24E-30SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.067567660S ribosome export
0.1482.29E-30SGTC_9761348-1067 47.6 μMChemDiv (Drug-like library)53106710.072463860S ribosome export
0.1452.78E-29SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.11267660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1628st00425277.7 μM0.541667692968TimTec (Natural product derivative library)257.28452.4713
SGTC_1971st06942963.5 μM0.4905661246522TimTec (Natural product derivative library)280.278082.5191460S ribosome export
SGTC_8810833-0569170 μM0.4807695418700ChemDiv (Drug-like library)306.315362.68414
SGTC_1910st06235260.4 μM0.4655172245730TimTec (Natural product derivative library)287.310481.94514
SGTC_1964st07691461 μM0.43859624208661TimTec (Natural product derivative library)327.761663.82714RPP1 & pyrimidine depletion
SGTC_1821st05346565.6 μM0.411765675086TimTec (Natural product derivative library)282.090121.81613
SGTC_1701st02863840.4 μM0.3802823490878TimTec (Natural product derivative library)494.546123.04638
SGTC_1914st06236772.6 μM0.375978205TimTec (Natural product derivative library)275.299781.98214
SGTC_9041141-0088396 μM0.3606561378119ChemDiv (Drug-like library)371.385384.14614
SGTC_2898905627058.44 μM0.35937519154699Chembridge (Drug-like library)352.38381.80215