7238770

N-(2-bromophenyl)pyrazine-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2205
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 279867
SMILES C1=CC=C(C(=C1)NC(=O)C2=NC=CN=C2)Br
Standardized SMILES Brc1ccccc1NC(=O)c2cnccn2
Molecular weight 278.1047
ALogP 1.49
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.14
% growth inhibition (Hom. pool) 4.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 279867
Download HIP data (tab-delimited text)  (excel)
Gene:CIA1(YDR267C)|FD-Score:3.39|P-value:3.48E-4|Clearance:0.22||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:DHR2(YKL078W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DPB11(YJL090C)|FD-Score:3.09|P-value:9.87E-4|Clearance:0.01||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:NAR1(YNL240C)|FD-Score:4.11|P-value:1.99E-5|Clearance:0.67||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:RPC10(YHR143W-A)|FD-Score:3.17|P-value:7.66E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL5(YPL131W)|FD-Score:-3.96|P-value:3.69E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:TIF34(YMR146C)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.04||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:CIA1(YDR267C)|FD-Score:3.39|P-value:3.48E-4|Clearance:0.22||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:DHR2(YKL078W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DPB11(YJL090C)|FD-Score:3.09|P-value:9.87E-4|Clearance:0.01||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:NAR1(YNL240C)|FD-Score:4.11|P-value:1.99E-5|Clearance:0.67||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:RPC10(YHR143W-A)|FD-Score:3.17|P-value:7.66E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPL5(YPL131W)|FD-Score:-3.96|P-value:3.69E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:TIF34(YMR146C)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.04||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 279867
Download HOP data (tab-delimited text)  (excel)
Gene:AIM11(YER093C-A)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:AVT1(YJR001W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CBF1(YJR060W)|FD-Score:-3.18|P-value:7.46E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FRA1(YLL029W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GYP6(YJL044C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HSL1(YKL101W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:KAP104(YBR017C)|FD-Score:-3.88|P-value:5.30E-5||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:LAC1(YKL008C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:MGM101(YJR144W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MUB1(YMR100W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NDL1(YLR254C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:PET112(YBL080C)|FD-Score:4.76|P-value:9.74E-7||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PIB2(YGL023C)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PPM1(YDR435C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PUS7(YOR243C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:SAT4(YCR008W)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SFP1(YLR403W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SIS2(YKR072C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKN7(YHR206W)|FD-Score:5.73|P-value:4.90E-9||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SPS18(YNL204C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SWT1(YOR166C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:UIP5(YKR044W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates Gene:VID22(YLR373C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA7(YGR020C)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS61(YDR136C_d)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:AIM11(YER093C-A)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:AVT1(YJR001W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CBF1(YJR060W)|FD-Score:-3.18|P-value:7.46E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FRA1(YLL029W)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GYP6(YJL044C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HSL1(YKL101W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:KAP104(YBR017C)|FD-Score:-3.88|P-value:5.30E-5||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:LAC1(YKL008C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:MGM101(YJR144W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MUB1(YMR100W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NDL1(YLR254C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:PET112(YBL080C)|FD-Score:4.76|P-value:9.74E-7||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PIB2(YGL023C)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PPM1(YDR435C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PUS7(YOR243C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:SAT4(YCR008W)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SFP1(YLR403W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SIS2(YKR072C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKN7(YHR206W)|FD-Score:5.73|P-value:4.90E-9||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SPS18(YNL204C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SWT1(YOR166C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:UIP5(YKR044W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates Gene:VID22(YLR373C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA7(YGR020C)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS61(YDR136C_d)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL240C4.111.99E-50.67NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YMR146C3.442.96E-40.04TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YDR267C3.393.48E-40.22CIA1Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
YHR143W-A3.177.66E-40.07RPC10RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress
YJL090C3.099.87E-40.01DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YDL164C3.080.001020.13CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YPR107C2.950.001580.05YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YKL186C2.900.001850.08MTR2mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
YOR224C2.830.002350.02RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YJR013W2.810.002480.13GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YDL217C2.680.003660.07TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YPR136C_d2.610.004490.01YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YIL106W2.610.004580.00MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YDR449C2.600.004610.05UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YMR047C2.550.005350.00NUP116FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR206W5.734.90E-9SKN7Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
YGR020C4.943.89E-7VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YBL080C4.769.74E-7PET112Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
YLL029W4.072.36E-5FRA1Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation
YGL005C3.963.75E-5COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YOR243C3.904.89E-5PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YLR373C3.542.00E-4VID22Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles
YLR403W3.522.16E-4SFP1Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
YJR144W3.442.96E-4MGM101Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage
YDR136C_d3.423.15E-4VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YER093C-A3.403.40E-4AIM11Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication
YKL101W3.363.90E-4HSL1Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p
YKR072C3.236.18E-4SIS2Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
YOR166C3.216.53E-4SWT1RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
YML071C3.216.54E-4COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments

GO enrichment analysis for SGTC_2205
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1318.44E-24SGTC_290299-0004 13.0 μMChemDiv (Drug-like library)168710.117647
0.1241.23E-21SGTC_2681menadione 3.2 μMMiscellaneous40550.125superoxide
0.1233.86E-21SGTC_1078idebenone 36.1 μMMiscellaneous36860.0333333Golgi
0.1133.36E-18SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.125Golgi
0.1103.43E-17SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.140351Golgi
0.1087.73E-17SGTC_3660181-0206 4.3 μMChemDiv (Drug-like library)2748740.108696Golgi
0.1071.43E-16SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.171875Golgi
0.1001.55E-14SGTC_20885278280 127.4 μMChembridge (Fragment library)7915020.0625
0.0992.27E-14SGTC_2766oltipraz 83.9 μMMiscellaneous473180.196078heme requiring
0.0993.16E-14SGTC_30579095946 49.5 μMChembridge (Drug-like library)245400430.0869565Golgi
0.0985.99E-14SGTC_6841140-0066 328.0 μMChemDiv (Drug-like library)68135340.0574713Golgi
0.0944.64E-13SGTC_24355706476 10.6 μMMiscellaneous57253750.106061
0.0945.97E-13SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.135593Golgi
0.0905.02E-12SGTC_1530rhein 70.4 μMTimTec (Pure natural product library)101680.12069
0.0899.56E-12SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.145455superoxide

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21545570129200 μM0.642857673366Chembridge (Fragment library)227.261821.68413
SGTC_21175521686200 μM0.545455803312Chembridge (Fragment library)227.261821.71413
SGTC_21505556613200 μM0.511111743190Chembridge (Fragment library)213.235241.22813
SGTC_21876125751200 μM0.488889874498Chembridge (Fragment library)235.1895861.15215RSC complex & mRNA processing
SGTC_2800788793581.82 μM0.3454552961002Chembridge (Drug-like library)340.826624.24613RPP1 & pyrimidine depletion
SGTC_525k781-267526.7 μM0.343651118ChemDiv (Drug-like library)320.14141.89723redox potentiating
SGTC_9861493-048384 μM0.3440079ChemDiv (Drug-like library)256.256681.31914
SGTC_20275150920200 μM0.339623786715Chembridge (Fragment library)273.310342.37334
SGTC_20485246248200 μM0.326531677046Chembridge (Fragment library)216.2110631.66913
SGTC_3233913234049.47 μM0.32203417682950Chembridge (Drug-like library)329.803983.08314