7283366

2-amino-4-thiophen-2-ylthiophene-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2208
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 898663
SMILES C1=CSC(=C1)C2=CSC(=C2C(=O)N)N
Standardized SMILES NC(=O)c1c(N)scc1c2cccs2
Molecular weight 224.3026
ALogP 1.34
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.21
% growth inhibition (Hom. pool) 4.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 898663
Download HIP data (tab-delimited text)  (excel)
Gene:NAR1(YNL240C)|FD-Score:5.05|P-value:2.22E-7|Clearance:1.98||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:RHO1(YPR165W)|FD-Score:-3.13|P-value:8.70E-4|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:SPB4(YFL002C)|FD-Score:-3.17|P-value:7.53E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:NAR1(YNL240C)|FD-Score:5.05|P-value:2.22E-7|Clearance:1.98||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:RHO1(YPR165W)|FD-Score:-3.13|P-value:8.70E-4|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:SPB4(YFL002C)|FD-Score:-3.17|P-value:7.53E-4|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 898663
Download HOP data (tab-delimited text)  (excel)
Gene:ABP1(YCR088W)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:AIM11(YER093C-A)|FD-Score:4.8|P-value:7.80E-7||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX7(YMR256C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CSG2(YBR036C)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:EXG1(YLR300W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:EXG2(YDR261C)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:EXO1(YOR033C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FIS1(YIL065C)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FUS2(YMR232W)|FD-Score:5.27|P-value:6.81E-8||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GCN3(YKR026C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GIT1(YCR098C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GSH2(YOL049W)|FD-Score:4.82|P-value:7.00E-7||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:LCL3(YGL085W_p)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:MPA43(YNL249C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MPC54(YOR177C)|FD-Score:5.86|P-value:2.31E-9||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:NEW1(YPL226W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NOP16(YER002W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NUP100(YKL068W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Gene:PER1(YCR044C)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PFK1(YGR240C)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO90(YJL198W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:POS5(YPL188W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:RCE1(YMR274C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RNP1(YLL046C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL31A(YDL075W)|FD-Score:-3.81|P-value:7.04E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SOP4(YJL192C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SSD1(YDR293C)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SYH1(YPL105C)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TOM70(YNL121C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:VPS70(YJR126C)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YCK3(YER123W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR101C(YCR101C_p)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YKL133C(YKL133C_p)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YMR196W(YMR196W_p)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR209C(YMR209C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR244W(YMR244W_p)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Putative protein of unknown function Gene:YNL134C(YNL134C_p)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YNL195C(YNL195C_p)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ABP1(YCR088W)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:AIM11(YER093C-A)|FD-Score:4.8|P-value:7.80E-7||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:-3.42|P-value:3.15E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX7(YMR256C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CSG2(YBR036C)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:EXG1(YLR300W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:EXG2(YDR261C)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:EXO1(YOR033C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FIS1(YIL065C)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FUS2(YMR232W)|FD-Score:5.27|P-value:6.81E-8||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GCN3(YKR026C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GIT1(YCR098C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GSH2(YOL049W)|FD-Score:4.82|P-value:7.00E-7||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:LCL3(YGL085W_p)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:MPA43(YNL249C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MPC54(YOR177C)|FD-Score:5.86|P-value:2.31E-9||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:NEW1(YPL226W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NOP16(YER002W)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NUP100(YKL068W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Gene:PER1(YCR044C)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PFK1(YGR240C)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO90(YJL198W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:POS5(YPL188W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:RCE1(YMR274C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RNP1(YLL046C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL31A(YDL075W)|FD-Score:-3.81|P-value:7.04E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SOP4(YJL192C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SSD1(YDR293C)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SYH1(YPL105C)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TOM70(YNL121C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:VPS70(YJR126C)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:YCK3(YER123W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR101C(YCR101C_p)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YKL133C(YKL133C_p)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YMR196W(YMR196W_p)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR209C(YMR209C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YMR244W(YMR244W_p)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Putative protein of unknown function Gene:YNL134C(YNL134C_p)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YNL195C(YNL195C_p)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL240C5.052.22E-71.98NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YBR109C3.070.001080.09CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YML126C2.980.001460.03ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YBR070C2.940.001620.00ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YDL205C2.940.001640.01HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YOR116C2.930.001690.05RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YER159C2.880.001980.04BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YPL082C2.840.002270.04MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YLR215C2.800.002550.07CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
YER013W2.730.003190.01PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YNL062C2.720.003290.16GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YBR256C2.560.005259.14E-4RIB5Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
YLR397C2.560.005270.00AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
YDL139C2.550.005340.15SCM3Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP
YFR052W2.400.008120.03RPN12Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR177C5.862.31E-9MPC54Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate
YMR244W_p5.561.33E-8YMR244W_pPutative protein of unknown function
YMR232W5.276.81E-8FUS2Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
YOL049W4.827.00E-7GSH2Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
YER093C-A4.807.80E-7AIM11Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication
YNL206C4.721.18E-6RTT106Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition
YMR196W_p4.385.86E-6YMR196W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
YDR261C4.082.25E-5EXG2Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YCR101C_p4.042.63E-5YCR101C_pPutative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene
YCR098C3.973.66E-5GIT1Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability
YJR126C3.816.85E-5VPS70Protein of unknown function involved in vacuolar protein sorting
YBR036C3.768.59E-5CSG2Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
YPL188W3.671.20E-4POS5Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress
YER123W3.571.76E-4YCK3Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
YMR209C_p3.542.02E-4YMR209C_pPutative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene

GO enrichment analysis for SGTC_2208
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0904.15E-12SGTC_22117117706 200.0 μMChembridge (Fragment library)8227710.14
0.0782.24E-9SGTC_22095-(5-methyl-2-thenylidene)-Barbituric acid 122.8 μMChembridge (Fragment library)6167640.0943396
0.0673.20E-7SGTC_28739040596 71.4 μMChembridge (Drug-like library)64638630.114286
0.0664.36E-7SGTC_22047233102 200.0 μMChembridge (Fragment library)21724800.11666760S ribosome export
0.0649.66E-7SGTC_32269131256 49.5 μMChembridge (Drug-like library)171780730.0945946
0.0622.25E-6SGTC_1833914-0051 30.6 μMChemDiv (Drug-like library)15962320.071428660S ribosome export
0.0613.16E-6SGTC_2252omeprazole 400.0 μMMiscellaneous45940.0540541
0.0604.58E-6SGTC_2581avocatin a 64.0 μMMicrosource (Natural product library)67107280.0491803sphingolipid biosynthesis & PDR1
0.0561.88E-5SGTC_1871st057448 67.3 μMTimTec (Natural product derivative library)6917470.0967742
0.0491.61E-4SGTC_2593nobiletin 86.2 μMMicrosource (Natural product library)723440.0634921
0.0482.03E-4SGTC_22737815444 169.1 μMChembridge (Fragment library)28246160.116667
0.0472.84E-4SGTC_274sirtinol 40.1 μMMiscellaneous68364420.115942
0.0473.15E-4SGTC_659k018-0002 107.0 μMChemDiv (Drug-like library)67978790.133333
0.0473.25E-4SGTC_33159136395 68.5 μMChembridge (Drug-like library)168768440.0789474
0.0454.88E-4SGTC_28349001755 58.4 μMChembridge (Drug-like library)29895750.15873

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22647948757110.76 μM0.3191492971411Chembridge (Fragment library)252.312721.35624
SGTC_21205538279151.97 μM0.3043482058197Chembridge (Fragment library)221.199551.88216
SGTC_2159565861173.75 μM0.288462793844Chembridge (Fragment library)289.35281.9234
SGTC_14954469-0029237 μM0.277778793680ChemDiv (Drug-like library)337.440342.98227
SGTC_2274794709446.08 μM0.2708332971028Chembridge (Fragment library)246.2851.40323
SGTC_6721441-001853.7 μM0.266667251141ChemDiv (Drug-like library)212.270323.19403
SGTC_21465545642200 μM0.265306825326Chembridge (Fragment library)202.232441.21713
SGTC_1982st07246771.9 μM0.2641513163533TimTec (Natural product derivative library)278.32513.99803
SGTC_1780st03844824.54 μM0.259259728868TimTec (Natural product derivative library)278.710883.21614iron homeostasis
SGTC_2061524588480.95 μM0.25573863Chembridge (Fragment library)210.276041.56514tubulin folding & SWR complex