5-(5-methyl-2-thenylidene)-Barbituric acid

5-[(5-methylthiophen-2-yl)methylidene]-1,3-diazinane-2,4,6-trione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2209
Screen concentration 122.8 μM
Source Chembridge (Fragment library)
PubChem CID 616764
SMILES CC1=CC=C(S1)C=C2C(=O)NC(=O)NC2=O
Standardized SMILES Cc1ccc(C=C2C(=O)NC(=O)NC2=O)s1
Molecular weight 236.2471
ALogP 0.96
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.87
% growth inhibition (Hom. pool) 12.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 616764
Download HIP data (tab-delimited text)  (excel)
Gene:ERG10(YPL028W)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.21||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:NAR1(YNL240C)|FD-Score:5.06|P-value:2.15E-7|Clearance:0.12||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NSA2(YER126C)|FD-Score:3.11|P-value:9.30E-4|Clearance:0.03||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:NSL1(YPL233W)|FD-Score:7.37|P-value:8.67E-14|Clearance:2.31||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:PFS2(YNL317W)|FD-Score:-3.27|P-value:5.39E-4|Clearance:0||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:RIB2(YOL066C)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.03||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPB10(YOR210W)|FD-Score:4.06|P-value:2.49E-5|Clearance:0.26||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL5(YPL131W)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP0(YLR340W)|FD-Score:4.14|P-value:1.73E-5|Clearance:0.04||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPS5(YJR123W)|FD-Score:-3.79|P-value:7.46E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC23(YPR181C)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SSL2(YIL143C)|FD-Score:4.93|P-value:4.02E-7|Clearance:0.79||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:TIF34(YMR146C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.33||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:UTP4(YDR324C)|FD-Score:3.8|P-value:7.37E-5|Clearance:0.08||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YBL077W(YBL077W_d)|FD-Score:4.07|P-value:2.39E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:ERG10(YPL028W)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.21||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:NAR1(YNL240C)|FD-Score:5.06|P-value:2.15E-7|Clearance:0.12||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NSA2(YER126C)|FD-Score:3.11|P-value:9.30E-4|Clearance:0.03||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:NSL1(YPL233W)|FD-Score:7.37|P-value:8.67E-14|Clearance:2.31||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:PFS2(YNL317W)|FD-Score:-3.27|P-value:5.39E-4|Clearance:0||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:RIB2(YOL066C)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.03||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPB10(YOR210W)|FD-Score:4.06|P-value:2.49E-5|Clearance:0.26||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL5(YPL131W)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP0(YLR340W)|FD-Score:4.14|P-value:1.73E-5|Clearance:0.04||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPS5(YJR123W)|FD-Score:-3.79|P-value:7.46E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC23(YPR181C)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SSL2(YIL143C)|FD-Score:4.93|P-value:4.02E-7|Clearance:0.79||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:TIF34(YMR146C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.33||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:UTP4(YDR324C)|FD-Score:3.8|P-value:7.37E-5|Clearance:0.08||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YBL077W(YBL077W_d)|FD-Score:4.07|P-value:2.39E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 616764
Download HOP data (tab-delimited text)  (excel)
Gene:ARC18(YLR370C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATC1(YDR184C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:CCS1(YMR038C)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC55(YGL190C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:COG6(YNL041C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSE1(YER124C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG3(YNL191W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ECM10(YEL030W)|FD-Score:3.43|P-value:2.96E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:ERS1(YCR075C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FMC1(YIL098C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:LAC1(YKL008C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:MRPL16(YBL038W)|FD-Score:-3.17|P-value:7.64E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL32(YCR003W)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:PPA2(YMR267W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PUF2(YPR042C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:PUS7(YOR243C)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD4(YER162C)|FD-Score:4.49|P-value:3.56E-6||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RKM3(YBR030W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RPN4(YDL020C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RXT3(YDL076C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAP155(YFR040W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SNT309(YPR101W)|FD-Score:4.96|P-value:3.53E-7||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOP4(YJL192C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRB5(YGR104C)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRN2(YLR119W)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSD1(YDR293C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWS2(YNL081C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TPN1(YGL186C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TUM1(YOR251C)|FD-Score:4.27|P-value:9.65E-6||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:VPS29(YHR012W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS72(YDR485C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL083C(YBL083C_d)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YGL117W(YGL117W_p)|FD-Score:-4.18|P-value:1.46E-5||SGD DESC:Putative protein of unknown function Gene:YGR242W(YGR242W_d)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YLR149C(YLR149C_p)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR007W(YMR007W_d)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR182W-A(YMR182W-A_p)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative protein of unknown function Gene:YNL010W(YNL010W_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOR114W(YOR114W_p)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL062W(YPL062W_d)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:ARC18(YLR370C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATC1(YDR184C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:CCS1(YMR038C)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC55(YGL190C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:COG6(YNL041C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSE1(YER124C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DUG3(YNL191W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ECM10(YEL030W)|FD-Score:3.43|P-value:2.96E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:ERS1(YCR075C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FMC1(YIL098C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:LAC1(YKL008C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:MRPL16(YBL038W)|FD-Score:-3.17|P-value:7.64E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL32(YCR003W)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:PPA2(YMR267W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PUF2(YPR042C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:PUS7(YOR243C)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD4(YER162C)|FD-Score:4.49|P-value:3.56E-6||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RKM3(YBR030W)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RPN4(YDL020C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RXT3(YDL076C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAP155(YFR040W)|FD-Score:3.96|P-value:3.78E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SNT309(YPR101W)|FD-Score:4.96|P-value:3.53E-7||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOP4(YJL192C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRB5(YGR104C)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRN2(YLR119W)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSD1(YDR293C)|FD-Score:-3.25|P-value:5.85E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWS2(YNL081C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TPN1(YGL186C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TUM1(YOR251C)|FD-Score:4.27|P-value:9.65E-6||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:VPS29(YHR012W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS72(YDR485C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL083C(YBL083C_d)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YGL117W(YGL117W_p)|FD-Score:-4.18|P-value:1.46E-5||SGD DESC:Putative protein of unknown function Gene:YGR242W(YGR242W_d)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YLR149C(YLR149C_p)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR007W(YMR007W_d)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR182W-A(YMR182W-A_p)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative protein of unknown function Gene:YNL010W(YNL010W_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOR114W(YOR114W_p)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPL062W(YPL062W_d)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL233W7.378.67E-142.31NSL1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YNL240C5.062.15E-70.12NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YIL143C4.934.02E-70.79SSL2Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
YLR340W4.141.73E-50.04RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YOL066C4.102.11E-50.03RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YBL077W_d4.072.39E-50.01YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YOR210W4.062.49E-50.26RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YDR324C3.807.37E-50.08UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YPL131W3.721.01E-40.06RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YMR146C3.651.29E-40.33TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YPL028W3.334.40E-40.21ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YER126C3.119.30E-40.03NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YGL103W3.090.001010.21RPL28Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance
YOR146W_d2.880.002010.01YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YFR037C2.870.002060.25RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR101W4.963.53E-7SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
YER162C4.493.56E-6RAD4Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
YOR243C4.464.04E-6PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YOR251C4.279.65E-6TUM1Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized
YDR129C4.211.29E-5SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YLR370C4.121.89E-5ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YGL186C4.052.59E-5TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YFR040W3.963.78E-5SAP155Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress
YER124C3.944.04E-5DSE1Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress
YMR182W-A_p3.924.37E-5YMR182W-A_pPutative protein of unknown function
YGR242W_d3.846.10E-5YGR242W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
YMR007W_d3.836.32E-5YMR007W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDL020C3.836.44E-5RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YNL191W3.711.02E-4DUG3Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
YHR012W3.701.10E-4VPS29Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval

GO enrichment analysis for SGTC_2209
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1424.53E-28SGTC_272doxorubicin 8.1 μMMiscellaneous317030.0638298anthracycline transcription coupled DNA repair
0.1262.41E-22SGTC_257daunorubicin 18.5 μMMiscellaneous303230.0659341anthracycline transcription coupled DNA repair
0.1132.64E-18SGTC_1085doxorubicin 7.7 μMNIH Clinical Collection4439390.0638298anthracycline transcription coupled DNA repair
0.1071.59E-16SGTC_246cycloheximide 667.0 μMICCB bioactive library167600400.105263
0.1073.26E-16SGTC_2662daunorubicin 22.4 μMMiscellaneous303230.0659341anthracycline transcription coupled DNA repair
0.1056.94E-16SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0566038
0.0985.73E-14SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0961538
0.0896.60E-12SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0784314
0.0832.21E-10SGTC_1110083-0039 52.5 μMChemDiv (Drug-like library)7593130.125
0.0815.35E-10SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0952381
0.0806.68E-10SGTC_1105epirubicin 18.1 μMNIH Clinical Collection653480.0638298anthracycline transcription coupled DNA repair
0.0782.24E-9SGTC_22087283366 200.0 μMChembridge (Fragment library)8986630.0943396
0.0758.06E-9SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.14tubulin folding & SWR complex
0.0741.65E-8SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.109375
0.0731.94E-8SGTC_23037774051 200.0 μMChembridge (Fragment library)29531840.116667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21365328288199.3 μM0.527778262395Chembridge (Fragment library)222.220540.81724
SGTC_2065522159634.26 μM0.390244788581Chembridge (Fragment library)238.286141.71724
SGTC_6033794-002345.9 μM0.3265313802260ChemDiv (Drug-like library)356.759823.09624
SGTC_8920929-007629 μM0.2727275751038ChemDiv (Drug-like library)219.259662.42813TRP & mitochondrial translation
SGTC_23067482397179.52 μM0.270833891448Chembridge (Fragment library)282.702882.18634RPP1 & pyrimidine depletion
SGTC_23276189656200 μM0.2708335349088Chembridge (Fragment library)282.702882.18634
SGTC_8063966-44555.86 μM0.254902714982ChemDiv (Drug-like library)316.3069633.17425
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.251241132ChemDiv (Drug-like library)227.32642.79414
SGTC_245553777129.75 μM0.252243908Miscellaneous219.259662.42813
SGTC_20405100729123 μM0.23809511840Chembridge (Fragment library)161.157381.15612