7173844

ethyl 2-(2,8-dimethyl-4-oxoquinolin-1-yl)acetate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2213
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 892728
SMILES CCOC(=O)CN1C(=CC(=O)C2=C1C(=CC=C2)C)C
Standardized SMILES CCOC(=O)CN1C(=CC(=O)c2cccc(C)c12)C
Molecular weight 259.3004
ALogP 2.62
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.01
% growth inhibition (Hom. pool) 9.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 892728
Download HIP data (tab-delimited text)  (excel)
Gene:RNR2(YJL026W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.25||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:TBF1(YPL128C)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.16||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:UTP22(YGR090W)|FD-Score:3.29|P-value:4.99E-4|Clearance:0.21||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:RNR2(YJL026W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.25||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:TBF1(YPL128C)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.16||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:UTP22(YGR090W)|FD-Score:3.29|P-value:4.99E-4|Clearance:0.21||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 892728
Download HOP data (tab-delimited text)  (excel)
Gene:AAH1(YNL141W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:ADR1(YDR216W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:API2(YDR525W_d)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ARO7(YPR060C)|FD-Score:5.3|P-value:5.80E-8||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG8(YBL078C)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BSC5(YNR069C)|FD-Score:4.4|P-value:5.47E-6||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BUD23(YCR047C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:DAL81(YIR023W)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCN1(YLR128W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DSF2(YBR007C_p)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERS1(YCR075C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FAA1(YOR317W)|FD-Score:3.75|P-value:8.83E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:GAC1(YOR178C)|FD-Score:4.3|P-value:8.65E-6||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GIN4(YDR507C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HFM1(YGL251C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HIS4(YCL030C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:IFA38(YBR159W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IRC6(YFR043C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:MKK1(YOR231W)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MND2(YIR025W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:NHP6A(YPR052C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NTH2(YBR001C)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAT1(YCR077C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PER1(YCR044C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PHO90(YJL198W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PIN4(YBL051C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RMD1(YDL001W)|FD-Score:4.3|P-value:8.47E-6||SGD DESC:Cytoplasmic protein required for sporulation Gene:RNH1(YMR234W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL35B(YDL136W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSC1(YGR056W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RUP1(YOR138C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SER1(YOR184W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SIS2(YKR072C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SYF2(YGR129W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRP1(YDR007W)|FD-Score:4.8|P-value:8.01E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.91|P-value:1.67E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.85|P-value:6.11E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL012C(YBL012C_d)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL083C(YBL083C_d)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCL042W(YCL042W_p)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR008C(YDR008C_d)|FD-Score:5.26|P-value:7.39E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL123W(YKL123W_d)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YLL059C(YLL059C_d)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YPL191C(YPL191C_p)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPT6(YLR262C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AAH1(YNL141W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:ADR1(YDR216W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:API2(YDR525W_d)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ARO7(YPR060C)|FD-Score:5.3|P-value:5.80E-8||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG8(YBL078C)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BSC5(YNR069C)|FD-Score:4.4|P-value:5.47E-6||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BUD23(YCR047C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:DAL81(YIR023W)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCN1(YLR128W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DSF2(YBR007C_p)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERS1(YCR075C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FAA1(YOR317W)|FD-Score:3.75|P-value:8.83E-5||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:GAC1(YOR178C)|FD-Score:4.3|P-value:8.65E-6||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GIN4(YDR507C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HFM1(YGL251C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HIS4(YCL030C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:IFA38(YBR159W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IRC6(YFR043C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:MKK1(YOR231W)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MND2(YIR025W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:NHP6A(YPR052C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NTH2(YBR001C)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAT1(YCR077C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PER1(YCR044C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PHO90(YJL198W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PIN4(YBL051C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RMD1(YDL001W)|FD-Score:4.3|P-value:8.47E-6||SGD DESC:Cytoplasmic protein required for sporulation Gene:RNH1(YMR234W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL35B(YDL136W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSC1(YGR056W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RUP1(YOR138C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SER1(YOR184W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SIS2(YKR072C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SYF2(YGR129W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRP1(YDR007W)|FD-Score:4.8|P-value:8.01E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.91|P-value:1.67E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.85|P-value:6.11E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL012C(YBL012C_d)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL083C(YBL083C_d)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCL042W(YCL042W_p)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR008C(YDR008C_d)|FD-Score:5.26|P-value:7.39E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL123W(YKL123W_d)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YLL059C(YLL059C_d)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YPL191C(YPL191C_p)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPT6(YLR262C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL026W3.701.07E-40.25RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YPL128C3.462.75E-40.16TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YGR090W3.294.99E-40.21UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YHR107C3.080.001040.08CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YDR081C3.000.001350.02PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YDL058W2.980.001430.00USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YKL078W2.980.001450.02DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YAL033W2.960.001550.03POP5Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR079W2.920.001740.03SEC14Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
YGR175C2.890.001940.01ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YFL039C2.880.002020.02ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YNL221C2.850.002160.03POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YPR144C2.820.002370.11NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YKL036C_d2.710.003320.15YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YLR166C2.570.005130.02SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER090W5.911.67E-9TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YPR060C5.305.80E-8ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YDR008C_d5.267.39E-8YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YIR023W4.983.18E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YKL211C4.856.11E-7TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR007W4.808.01E-7TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YBL078C4.711.25E-6ATG8Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
YNR069C4.405.47E-6BSC5Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro
YLL059C_d4.347.18E-6YLL059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDL001W4.308.47E-6RMD1Cytoplasmic protein required for sporulation
YOR178C4.308.65E-6GAC1Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock
YML012C-A_d4.289.26E-6YML012C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
YGR056W4.191.37E-5RSC1Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
YBL008W-A_p3.885.22E-5YBL008W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YBR001C3.768.64E-5NTH2Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2213
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2776.99E-104SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.136364
0.2712.07E-99SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0909091
0.2551.31E-87SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.171875
0.2531.63E-86SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0337079
0.2493.42E-84SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.104478
0.2422.30E-79SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.109091
0.2381.87E-76SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.0985916
0.2301.14E-71SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.126761
0.2298.57E-71SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.015873
0.2265.79E-69SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.121622
0.2218.22E-66SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.155844
0.2183.00E-64SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.0735294
0.2171.12E-63SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.152542
0.2115.01E-60SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0547945
0.2107.73E-60SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.215385

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2828799886171.43 μM0.3333332988188Chembridge (Drug-like library)325.358463.0404
SGTC_2874904390471.43 μM0.3333334463428Chembridge (Drug-like library)312.38460.9606
SGTC_23569061756188.97 μM0.32203420104043Chembridge (Fragment library)246.304921.46312
SGTC_1110083-003952.46 μM0.321429759313ChemDiv (Drug-like library)238.281163.55902
SGTC_1838st05536984.5 μM0.296296676036TimTec (Natural product derivative library)236.265283.62202
SGTC_1924st05845975.1 μM0.293103688683TimTec (Natural product derivative library)266.291263.60603
SGTC_800159-0065512.24 μM0.2857141548079ChemDiv (Drug-like library)390.431784.49814
SGTC_12220366-00741.18 μM0.277778286520ChemDiv (Drug-like library)245.230741.59204TSC3-RPN4
SGTC_2681menadione3.18 μM0.2765964055Miscellaneous172.180022.20402superoxide
SGTC_394menadione8.33 μM0.2765964055Miscellaneous172.180022.20402superoxide
SGTC_2113455-0679143.5 μM0.271186748649ChemDiv (Drug-like library)220.221282.87314