7188065

4-chloro-6-methylquinazoline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2215
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 940217
SMILES CC1=CC2=C(C=C1)N=CN=C2Cl
Standardized SMILES Cc1ccc2ncnc(Cl)c2c1
Molecular weight 178.6183
ALogP 2.75
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 1.16


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 940217
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.45||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CDC31(YOR257W)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:RPF1(YHR088W)|FD-Score:4.39|P-value:5.60E-6|Clearance:0.71||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL15A(YLR029C)|FD-Score:4.43|P-value:4.65E-6|Clearance:0.71||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP0(YLR340W)|FD-Score:3.23|P-value:6.18E-4|Clearance:0.08||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RSC9(YML127W)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:YRA1(YDR381W)|FD-Score:3.15|P-value:8.17E-4|Clearance:0.17||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:AFG2(YLR397C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.45||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CDC31(YOR257W)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:RPF1(YHR088W)|FD-Score:4.39|P-value:5.60E-6|Clearance:0.71||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL15A(YLR029C)|FD-Score:4.43|P-value:4.65E-6|Clearance:0.71||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP0(YLR340W)|FD-Score:3.23|P-value:6.18E-4|Clearance:0.08||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RSC9(YML127W)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:YRA1(YDR381W)|FD-Score:3.15|P-value:8.17E-4|Clearance:0.17||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 940217
Download HOP data (tab-delimited text)  (excel)
Gene:EAP1(YKL204W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECM19(YLR390W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV8(YGR196C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:HAP4(YKL109W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HSV2(YGR223C)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HUB1(YNR032C-A)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:IRA2(YOL081W)|FD-Score:4.86|P-value:5.90E-7||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:JID1(YPR061C)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LDB7(YBL006C)|FD-Score:6.21|P-value:2.62E-10||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MRL1(YPR079W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress Gene:OSM1(YJR051W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PET20(YPL159C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:RPL34B(YIL052C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS26B(YER131W)|FD-Score:4.37|P-value:6.28E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RUD3(YOR216C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAC6(YDR129C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDP1(YIL113W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SEC28(YIL076W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SOH1(YGL127C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SWR1(YDR334W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TPM1(YNL079C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:VNX1(YNL321W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YMR187C(YMR187C_p)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YPL114W(YPL114W_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPL191C(YPL191C_p)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EAP1(YKL204W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECM19(YLR390W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV8(YGR196C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:HAP4(YKL109W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HSV2(YGR223C)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HUB1(YNR032C-A)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear Gene:IRA2(YOL081W)|FD-Score:4.86|P-value:5.90E-7||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:JID1(YPR061C)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LDB7(YBL006C)|FD-Score:6.21|P-value:2.62E-10||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MRL1(YPR079W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress Gene:OSM1(YJR051W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PET20(YPL159C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:RPL34B(YIL052C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS26B(YER131W)|FD-Score:4.37|P-value:6.28E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RUD3(YOR216C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAC6(YDR129C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDP1(YIL113W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SEC28(YIL076W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SOH1(YGL127C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SWR1(YDR334W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TPM1(YNL079C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:VNX1(YNL321W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YJR005C-A(YJR005C-A_p)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YMR187C(YMR187C_p)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YPL114W(YPL114W_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPL191C(YPL191C_p)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR029C4.434.65E-60.71RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YHR088W4.395.60E-60.71RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YLR397C3.681.15E-40.45AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
YLR340W3.236.18E-40.08RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YDR381W3.158.17E-40.17YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YIR011C2.980.001430.08STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YDR160W2.900.001850.09SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YHR083W2.810.002450.14SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YDR328C2.670.003760.02SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YBR004C2.650.003980.06GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YJL111W2.590.004800.03CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YOL022C2.560.005220.02TSR4Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
YMR270C2.540.005550.04RRN9Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
YNR026C2.500.006160.02SEC12Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
YDL132W2.480.006590.05CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL006C6.212.62E-10LDB7Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
YOL081W4.865.90E-7IRA2GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication
YER131W4.376.28E-6RPS26BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication
YPR061C4.032.73E-5JID1Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
YIL076W3.944.10E-5SEC28Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress
YMR187C_p3.924.50E-5YMR187C_pPutative protein of unknown function; YMR187C is not an essential gene
YGL127C3.681.15E-4SOH1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
YPL193W3.641.35E-4RSA1Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
YPL159C3.591.67E-4PET20Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome
YPL114W_d3.551.93E-4YPL114W_dDubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index
YLR390W3.482.53E-4ECM19Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YKL109W3.442.94E-4HAP4Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
YIL052C3.423.11E-4RPL34BRibosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
YIL113W3.423.15E-4SDP1Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock
YJR005C-A_p3.383.58E-4YJR005C-A_pPutative protein of unknown function; originally identified as a syntenic homolog of an Ashbya gossypii gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2215
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0977.75E-14SGTC_8630466-0191 129.0 μMChemDiv (Drug-like library)7651600.0714286cell wall
0.0937.49E-13SGTC_1104tacrolimus 29.8 μMNIH Clinical Collection235817960.0357143
0.0831.50E-10SGTC_1681tripelennamine 100.0 μMNIH Clinical Collection90660.12
0.0822.77E-10SGTC_21925915882 198.5 μMChembridge (Fragment library)28761490.0555556RNA processing & uracil transport
0.0822.97E-10SGTC_2412st077135 33.4 μMTimTec (Natural product derivative library)132589150.0632911
0.0815.79E-10SGTC_2628lathosterol 100.0 μMMicrosource (Natural product library)67106160.0434783
0.0781.80E-9SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.104478cell wall
0.0765.28E-9SGTC_2656pseudo-anisatin 100.0 μMMicrosource (Natural product library)67088090.0327869
0.0741.35E-8SGTC_22287354201 200.0 μMChembridge (Fragment library)7534510.0535714endomembrane recycling
0.0741.55E-8SGTC_30169080866 49.5 μMChembridge (Drug-like library)170662990.101449
0.0707.24E-8SGTC_7924534-1957 155.0 μMChemDiv (Drug-like library)17179040.101695
0.0681.69E-7SGTC_29237992654 58.1 μMChembridge (Drug-like library)29851260.0677966Golgi
0.0663.89E-7SGTC_20815214970 32.3 μMChembridge (Fragment library)20635120.0740741
0.0664.20E-7SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.0877193RNA processing & uracil transport
0.0631.24E-6SGTC_30799117512 49.5 μMChembridge (Drug-like library)71850440.112903

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3273913785949.47 μM0.3488372377234Chembridge (Drug-like library)266.360764.53803
SGTC_6074491-098666.8 μM0.347826698981ChemDiv (Drug-like library)296.793985.48512heme requiring
SGTC_20695228469200 μM0.3181822835210Chembridge (Fragment library)215.254421.64223
SGTC_14023909-767335.9 μM0.2888895328014ChemDiv (Drug-like library)269.728883.98313
SGTC_13151237-00193.69 μM0.27907825140ChemDiv (Drug-like library)246.26343.7303
SGTC_9923909-8734162 μM0.2765964288319ChemDiv (Drug-like library)275.75664.01114
SGTC_9933909-875762.7 μM0.2653064288324ChemDiv (Drug-like library)275.75664.01114
SGTC_9871497-1013404 μM0.2592591676435ChemDiv (Drug-like library)362.87526.58603
SGTC_8380109-020371.4 μM0.255814759422ChemDiv (Drug-like library)208.258483.75611
SGTC_730327-032516.23 μM0.2553298ChemDiv (Drug-like library)284.354445.38912DNA damage response