nsc-310342

[3-(4-chlorophenyl)-1,2,4-oxadiazol-5-yl]methyl carbamimidothioate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_222
Screen concentration 10.7 μM
Source Miscellaneous
PubChem CID 432556
SMILES C1=CC(=CC=C1C2=NOC(=N2)CSC(=N)N)Cl
Standardized SMILES NC(=N)SCc1onc(n1)c2ccc(Cl)cc2
Molecular weight 268.7227
ALogP 2.26
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.56
% growth inhibition (Hom. pool) 1.3


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 432556
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:-3.15|P-value:8.06E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:RBA50(YDR527W)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.31||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:SRB7(YDR308C)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:CCT7(YJL111W)|FD-Score:-3.15|P-value:8.06E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:RBA50(YDR527W)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.31||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:SRB7(YDR308C)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 432556
Download HOP data (tab-delimited text)  (excel)
Gene:BNI4(YNL233W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:CTR1(YPR124W)|FD-Score:-5.4|P-value:3.40E-8||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYT2(YKL087C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DAL81(YIR023W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EMC5(YIL027C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FMP40(YPL222W_p)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA2(YGL220W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GAD1(YMR250W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:MAC1(YMR021C)|FD-Score:-4.82|P-value:7.29E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDH1(YKL085W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:PSY2(YNL201C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:ROX3(YBL093C)|FD-Score:4|P-value:3.17E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPN4(YDL020C)|FD-Score:5.7|P-value:5.90E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR444W(YDR444W_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YLR271W(YLR271W_p)|FD-Score:8.2|P-value:1.25E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YNL295W(YNL295W_p)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Putative protein of unknown function Gene:YNR064C(YNR064C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YPL113C(YPL113C_p)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:-3.81|P-value:6.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YRA2(YKL214C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:BNI4(YNL233W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:CTR1(YPR124W)|FD-Score:-5.4|P-value:3.40E-8||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYT2(YKL087C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DAL81(YIR023W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EMC5(YIL027C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FMP40(YPL222W_p)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA2(YGL220W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GAD1(YMR250W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:MAC1(YMR021C)|FD-Score:-4.82|P-value:7.29E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDH1(YKL085W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:PSY2(YNL201C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:ROX3(YBL093C)|FD-Score:4|P-value:3.17E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPN4(YDL020C)|FD-Score:5.7|P-value:5.90E-9||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR444W(YDR444W_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YLR271W(YLR271W_p)|FD-Score:8.2|P-value:1.25E-16||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YNL295W(YNL295W_p)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Putative protein of unknown function Gene:YNR064C(YNR064C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YPL113C(YPL113C_p)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:-3.81|P-value:6.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YRA2(YKL214C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR527W3.265.51E-40.31RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YHR062C2.950.001580.04RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR267C2.910.001820.08FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YPR034W2.820.002370.08ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YIL104C2.740.003040.13SHQ1Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
YBR055C2.610.004510.10PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YBR089W_d2.510.006080.04YBR089W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
YLR212C2.470.006730.00TUB4Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
YDL193W2.470.006770.09NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YBR196C2.380.008740.00PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YPL122C2.370.008860.04TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YJL009W_d2.330.009900.00YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YBR202W2.330.009960.03MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YER006W2.300.010800.00NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YHR023W2.290.010900.02MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR271W_p8.201.25E-16YLR271W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YDL020C5.705.90E-9RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YBL093C4.003.17E-5ROX3Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
YGL220W3.571.81E-4FRA2Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YNL233W3.432.98E-4BNI4Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p
YKL087C3.383.67E-4CYT2Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
YKL085W3.363.87E-4MDH1Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
YIR023W3.324.45E-4DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YKL214C3.295.06E-4YRA2Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus
YDR444W_p3.285.15E-4YDR444W_pPutative hydrolase acting on ester bonds
YIL027C3.275.45E-4EMC5Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT
YNL201C3.206.91E-4PSY2Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
YNL295W_p3.177.70E-4YNL295W_pPutative protein of unknown function
YNR064C3.148.39E-4YNR064CEpoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides
YBR185C3.080.00104MBA1Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane

GO enrichment analysis for SGTC_222
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2113.55E-60SGTC_223busulfan 5.0 mMMiscellaneous24780.02
0.2062.22E-57SGTC_220paclitaxel 373.4 μMMiscellaneous46660.0423729
0.2011.41E-54SGTC_225nsc-122657 1.0 μMMiscellaneous2756850.0606061
0.1962.02E-52SGTC_224benfluorex 43.9 μMMiscellaneous23180.0649351
0.1857.19E-47SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0655738
0.1484.12E-30SGTC_1069diphenylcyclopropenone 8.5 μMNIH Clinical Collection650570.0851064
0.1476.88E-30SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0862069
0.1418.81E-28SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0757576
0.1411.02E-27SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.0675676
0.1402.19E-27SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0943396
0.1382.10E-26SGTC_12460422-0085 105.0 μMChemDiv (Drug-like library)2642950.0535714
0.1271.20E-22SGTC_2115tiliquinol 12.8 μMChembridge (Fragment library)712080.0701754
0.1263.60E-22SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0655738
0.1223.80E-21SGTC_1791273-0063 6.8 μMChemDiv (Drug-like library)31081390.0434783
0.1211.19E-20SGTC_31699102970 49.5 μMChembridge (Drug-like library)170278390.125RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3012908043371.43 μM0.4528317688859Chembridge (Drug-like library)321.80193.26604
SGTC_2028515389036.43 μM0.3695652830968Chembridge (Fragment library)271.594463.71832cell wall signaling
SGTC_21275253358187.91 μM0.35714320612Chembridge (Fragment library)221.646321.77825
SGTC_13611608-00415.61 μM0.346154X1361ChemDiv (Drug-like library)294.778242.29552RPP1 & pyrimidine depletion
SGTC_20194016004108 μM0.29166745595336Chembridge (Fragment library)295.743161.06545
SGTC_7814335-280694.4 μM0.288136845254ChemDiv (Drug-like library)281.30922.80205
SGTC_7441594-0068283 μM0.25668392ChemDiv (Drug-like library)211.671342.14614amide catabolism
SGTC_2997906707571.43 μM0.24590227209819Chembridge (Drug-like library)297.2837832.62315unfolded protein response
SGTC_1983454-287316.19 μM0.245614710957ChemDiv (Drug-like library)297.782044.7461360S ribosome export
SGTC_3015908084549.47 μM0.2424245296973Chembridge (Drug-like library)345.35472.14415